Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,689,267 C→A 100% I36I (ATC→ATA)  mltF → membrane‑bound lytic transglycosylase F, murein hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,689,2670CA97.6% 905.5 / 10.6 292I36I (ATC→ATA) mltFmembrane‑bound lytic transglycosylase F, murein hydrolase
Reads supporting (aligned to +/- strand):  ref base C (6/1);  new base A (152/131);  total (158/134)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 297 total aligned reads displayed.

TTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGACCG  >  CP009273/2689121‑2689415
                                                                                                                                                  |                                                                                                                                                    
  tCAAGCTGGGACGCGCACGACACAGAGTATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATagccgc                                                                                                                                                 <  2:2911815/150‑1 (MQ=255)
  tCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATagccgc                                                                                                                                                 <  2:2195163/150‑1 (MQ=255)
     aGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCAt                                                                                                                                              >  1:4974403/1‑150 (MQ=255)
      gCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTTAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCAtt                                                                                                                                             <  1:3135544/150‑1 (MQ=255)
      gCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCCTCCATTCCCTGGTTTGGTAAAGCCGACAACCCTATAGCCGCCaat                                                                                                                                             >  1:1385942/1‑148 (MQ=255)
       cTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTc                                                                                                                                            >  2:5160920/1‑150 (MQ=255)
       cTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTc                                                                                                                                            >  1:2095854/1‑150 (MQ=255)
        tGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGNTAAAGCCGACAACCGTATAGCCGCCATTCa                                                                                                                                           >  2:4248971/1‑150 (MQ=255)
              gcgcACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGcgcg                                                                                                                                     >  2:4114687/1‑150 (MQ=255)
                  aagACACAGATAATTAACTAATTGAAATAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGga                                                                                                                                 <  2:1683833/148‑1 (MQ=255)
                  aCGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGga                                                                                                                                 <  1:3255426/150‑1 (MQ=255)
                   cGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGgag                                                                                                                                <  1:1869828/150‑1 (MQ=255)
                    gACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGgaga                                                                                                                               <  1:603081/150‑1 (MQ=255)
                      cacaGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGt                                                                                                                             >  1:3936003/1‑150 (MQ=255)
                      cacaGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGt                                                                                                                             >  1:355189/1‑150 (MQ=255)
                       acaGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGtt                                                                                                                            <  2:1017187/150‑1 (MQ=255)
                          gagaATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCg                                                                                                                         <  2:1740717/150‑1 (MQ=255)
                           agaATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCgt                                                                                                                        >  2:3361538/1‑150 (MQ=255)
                           agaATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCgt                                                                                                                        >  2:2337382/1‑150 (MQ=255)
                              aTTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGtgtg                                                                                                                     >  1:4847310/1‑150 (MQ=255)
                               ttAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGa                                                                                                                    <  1:3422720/150‑1 (MQ=255)
                                tAACTAATTGAAAAAATTAAATATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGTGAGAGTTGCGTGTGAg                                                                                                                   <  2:3107924/150‑1 (MQ=255)
                                 aaCTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGc                                                                                                                  >  1:2606561/1‑150 (MQ=255)
                                 aaCTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCACTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGc                                                                                                                  <  1:961821/150‑1 (MQ=255)
                                    tAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCAcc                                                                                                               <  2:1818079/150‑1 (MQ=255)
                                     aaTTGAAAAAATTTAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCTCGGGGAGAGTTGCGTGTGAGCACCa                                                                                                              <  2:1332653/150‑1 (MQ=255)
                                     aaTTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCa                                                                                                              >  1:5112342/1‑150 (MQ=255)
                                          aaaaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCAt                                                                                                         >  2:1356807/1‑150 (MQ=255)
                                            aaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATAc                                                                                                       <  2:2767019/150‑1 (MQ=255)
                                             aaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACt                                                                                                      <  2:2733613/150‑1 (MQ=255)
                                               aTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcc                                                                                                    >  1:1080531/1‑150 (MQ=255)
                                                ttAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTccc                                                                                                   <  1:1681030/150‑1 (MQ=255)
                                                 tAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcccc                                                                                                  >  2:506607/1‑150 (MQ=255)
                                                     gATTAATTATCTGTTCATCGGCATTCTGGCTCTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGAc                                                                                              <  1:539371/150‑1 (MQ=255)
                                                      attaattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACt                                                                                             <  1:447837/150‑1 (MQ=255)
                                                      attaattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACt                                                                                             <  1:4913237/150‑1 (MQ=255)
                                                       ttaattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACtt                                                                                            <  2:3271636/150‑1 (MQ=255)
                                                        taattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTta                                                                                           >  2:3056667/1‑150 (MQ=255)
                                                        taattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTta                                                                                           >  2:2516442/1‑150 (MQ=255)
                                                        taattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACACCCCTGACTta                                                                                           >  2:479939/1‑150 (MQ=255)
                                                         aattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTtat                                                                                          >  2:771221/1‑150 (MQ=255)
                                                         aattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTtat                                                                                          <  2:1115722/150‑1 (MQ=255)
                                                          attaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTtata                                                                                         <  1:3661107/150‑1 (MQ=255)
                                                          attaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTtata                                                                                         >  2:2433467/1‑150 (MQ=255)
                                                          attaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTtata                                                                                         >  2:4598371/1‑150 (MQ=255)
                                                          attaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTtata                                                                                         >  2:2370520/1‑150 (MQ=255)
                                                           ttaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATaa                                                                                        >  2:1286227/1‑150 (MQ=255)
                                                            taTCTGTTCATCGGCATTCTGGCTCTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAAc                                                                                       <  2:4357813/150‑1 (MQ=255)
                                                            taTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAAc                                                                                       >  2:955556/1‑150 (MQ=255)
                                                            taTCTGTTCATCGGAATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAAc                                                                                       <  1:1506856/150‑1 (MQ=255)
                                                            taTCTGTTCATCGGAATTCTGGCACTGATGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAATGCCGACAACCGTATAGCCGCCATTCAATCGCGGGGAGATTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAAc                                                                                       <  2:3946974/150‑1 (MQ=255)
                                                             aTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTCTAACg                                                                                      >  2:2051780/1‑150 (MQ=255)
                                                             aTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACg                                                                                      <  2:3806787/150‑1 (MQ=255)
                                                             aTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACg                                                                                      >  2:4026607/1‑150 (MQ=255)
                                                             aTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACg                                                                                      >  2:4403724/1‑150 (MQ=255)
                                                             aTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCAGACTTATAACg                                                                                      >  1:4276913/1‑150 (MQ=255)
                                                                tGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGaaa                                                                                   >  2:1505335/1‑150 (MQ=255)
                                                                 gTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAAt                                                                                  <  1:2413988/150‑1 (MQ=255)
                                                                 gTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGGATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAAt                                                                                  >  1:4156446/1‑150 (MQ=255)
                                                                  ttCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACACCCCTGACTTATAACGAAATc                                                                                 >  2:1915010/1‑150 (MQ=255)
                                                                    cATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCaa                                                                               >  2:3508043/1‑150 (MQ=255)
                                                                    cATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCAGGCCATACATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCAATCAAGCGCGGGGAGAGTTGCGTGTGAGCACCAGTCATACACCCCTGACATATAACGAAAACaa                                                                               >  1:1315686/1‑150 (MQ=255)
                                                                     aTCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAAc                                                                              >  2:1480744/1‑150 (MQ=255)
                                                                     aTCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGACATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAAc                                                                              <  2:3393454/150‑1 (MQ=255)
                                                                      tCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACg                                                                             <  1:55588/150‑1 (MQ=255)
                                                                        ggCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACggg                                                                           >  2:2657087/1‑150 (MQ=255)
                                                                          cATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGac                                                                         >  2:4921555/1‑149 (MQ=255)
                                                                           aTTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTTGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGGTGCGTGTGAGCACCAGTCATACTCCCCTGACTTATAACGAAATAAACGGGaca                                                                        >  1:1089712/1‑148 (MQ=255)
                                                                             tCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAAcc                                                                      <  1:1663648/150‑1 (MQ=255)
                                                                                ggCACTGCTGCTCGCGGTCGCTCTCTGGCCATACATTCCCTGGTTTGGTAAAGCCGACAACCGTAAAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACATATAACGAAATCAACGGGAAACCttt                                                                   >  1:2936679/1‑150 (MQ=255)
                                                                                 gCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCtttt                                                                  <  1:874322/150‑1 (MQ=255)
                                                                                           tCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGAtt                                                        <  1:1226293/150‑1 (MQ=255)
                                                                                             gcgGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTAc                                                      <  1:4123143/150‑1 (MQ=255)
                                                                                                 tCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAAc                                                  >  1:790675/1‑150 (MQ=255)
                                                                                                  cGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACt                                                 >  2:1370805/1‑150 (MQ=255)
                                                                                                  cGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACt                                                 <  1:2376369/150‑1 (MQ=255)
                                                                                                  cGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACt                                                 >  2:635702/1‑150 (MQ=255)
                                                                                                   gCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTg                                                >  2:3849800/1‑150 (MQ=255)
                                                                                                    ctctctGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTgg                                               <  2:529084/150‑1 (MQ=255)
                                                                                                         tGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGaaa                                          >  1:4690051/1‑150 (MQ=255)
                                                                                                         tGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGaaa                                          <  1:2358527/150‑1 (MQ=255)
                                                                                                          ggCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAAc                                         >  1:4854869/1‑150 (MQ=255)
                                                                                                           gCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACa                                        >  2:1414937/1‑150 (MQ=255)
                                                                                                                ccatTCCCTGGTTTGGTAAAGCCGACAACCGTATAGACGACATTCATTCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTg                                   <  2:2012259/150‑1 (MQ=255)
                                                                                                                   ttCCCTTGTTTGGTAAATCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCg                                <  1:2159666/150‑1 (MQ=255)
                                                                                                                    tCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGa                               >  1:4663865/1‑150 (MQ=255)
                                                                                                                     cccTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGAt                              <  2:2865829/150‑1 (MQ=255)
                                                                                                                      ccTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGAtt                             >  1:4972602/1‑150 (MQ=255)
                                                                                                                           tttGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTc                        <  2:4847340/150‑1 (MQ=255)
                                                                                                                            ttGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCg                       <  1:1383620/150‑1 (MQ=255)
                                                                                                                                   aGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTaaa                >  1:2974948/1‑150 (MQ=255)
                                                                                                                                     ccGACAACCGTATAGCCGCCATTCAAGCGCGGTGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAAc              <  2:4498335/150‑1 (MQ=255)
                                                                                                                                       gACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTg            <  1:1984238/150‑1 (MQ=255)
                                                                                                                                          aaCCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGaaa         >  2:3539799/1‑150 (MQ=255)
                                                                                                                                          aaCCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGaaa         >  2:866566/1‑150 (MQ=255)
                                                                                                                                           aCCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAg        <  2:1723625/150‑1 (MQ=255)
                                                                                                                                             cGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTg      <  1:4336623/150‑1 (MQ=255)
                                                                                                                                             cGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTg      <  1:3796532/150‑1 (MQ=255)
                                                                                                                                              gTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGa     <  1:2887613/150‑1 (MQ=255)
                                                                                                                                               tataGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGAc    >  1:3501690/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGACCG  >  CP009273/2689121‑2689415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑