Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,694,843 G→T 100% I82I (ATC→ATA)  pdxJ ← pyridoxine 5'‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,694,8430GT97.3% 933.3 / 13.0 298I82I (ATC→ATA) pdxJpyridoxine 5'‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (5/3);  new base T (171/119);  total (176/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 299 total aligned reads displayed.

AGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGC  >  CP009273/2694694‑2694989
                                                                                                                                                     |                                                                                                                                                  
   gaAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATc                                                                                                                                                 <  1:2158478/150‑1 (MQ=255)
   gaAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATc                                                                                                                                                 >  2:4207936/1‑150 (MQ=255)
    aaaCCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCg                                                                                                                                                <  1:2848613/150‑1 (MQ=255)
      aCCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGcc                                                                                                                                              >  1:3922206/1‑150 (MQ=255)
        cTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTCCCAGGCCGCAAAATTGTGGCTTCGTCTCAGCGGCTCTCGCCAg                                                                                                                                            >  2:5135174/1‑150 (MQ=255)
           aaTCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAt                                                                                                                                         >  2:2997319/1‑150 (MQ=255)
                 ggCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTc                                                                                                                                   <  1:65185/150‑1 (MQ=255)
                  gCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCg                                                                                                                                  <  2:2618679/150‑1 (MQ=255)
                   cATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCgg                                                                                                                                 >  1:1150331/1‑150 (MQ=255)
                   cATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCgg                                                                                                                                 <  1:691701/150‑1 (MQ=255)
                    aTCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGt                                                                                                                                >  2:3291310/1‑150 (MQ=255)
                    aTCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGt                                                                                                                                >  1:1796670/1‑150 (MQ=255)
                    aTCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGt                                                                                                                                >  2:2274818/1‑150 (MQ=255)
                    aTCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGt                                                                                                                                >  1:4679951/1‑150 (MQ=255)
                     tCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTc                                                                                                                               <  2:1097559/150‑1 (MQ=255)
                        gCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCAcc                                                                                                                            <  2:3585504/150‑1 (MQ=255)
                              cGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCAtc                                                                                                                      <  2:4478657/150‑1 (MQ=255)
                                tttGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCAtctc                                                                                                                    >  1:4623085/1‑150 (MQ=255)
                                 ttGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTcc                                                                                                                   >  1:1468157/1‑150 (MQ=255)
                                      ggCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGAtt                                                                                                              <  1:1357111/150‑1 (MQ=255)
                                      ggCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGAtt                                                                                                              >  2:1516619/1‑150 (MQ=255)
                                      ggCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGAtt                                                                                                              <  2:1572679/150‑1 (MQ=255)
                                         aTCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCAt                                                                                                           >  2:2801240/1‑150 (MQ=255)
                                             cgcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCCTCTCCAGATTCATGCgg                                                                                                       >  2:451703/1‑150 (MQ=255)
                                             cgcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCgg                                                                                                       >  2:956691/1‑150 (MQ=255)
                                             cgcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCgg                                                                                                       <  1:1134872/150‑1 (MQ=255)
                                              gcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCggg                                                                                                      >  2:1664492/1‑150 (MQ=255)
                                              gcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCggg                                                                                                      >  2:864719/1‑150 (MQ=255)
                                               cATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGt                                                                                                     <  1:1541802/150‑1 (MQ=255)
                                               cATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGt                                                                                                     >  1:4262655/1‑150 (MQ=255)
                                                 ttttGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTAt                                                                                                   <  1:1307800/150‑1 (MQ=255)
                                                         cGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTc                                                                                           >  2:336924/1‑150 (MQ=255)
                                                          gCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCt                                                                                          >  2:4391130/1‑150 (MQ=255)
                                                          gCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCt                                                                                          <  2:3645549/150‑1 (MQ=255)
                                                             gCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGAc                                                                                       >  2:1950660/1‑150 (MQ=255)
                                                              cccTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACg                                                                                      >  1:3308722/1‑150 (MQ=255)
                                                                 tGCGACATCCAGGCCGCCTTCGGTTGTTTCTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAg                                                                                   <  1:3781185/150‑1 (MQ=255)
                                                                  gCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAgg                                                                                  <  2:4928558/150‑1 (MQ=255)
                                                                    gACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGAt                                                                                <  2:1545549/150‑1 (MQ=255)
                                                                       aTCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATgcg                                                                             >  2:3989950/1‑150 (MQ=255)
                                                                       aTCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATgcg                                                                             <  2:4102827/150‑1 (MQ=255)
                                                                       aTCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATgcg                                                                             >  2:3413483/1‑150 (MQ=255)
                                                                        tCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATgcgc                                                                            >  2:3353419/1‑150 (MQ=255)
                                                                           aGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCAACGCCATCTCCAGATTCATTCGGGTATCCAGCGTCTGACGCAGGATGCGCACg                                                                         >  2:1618597/1‑150 (MQ=255)
                                                                               cgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGACAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                                                     >  1:413289/1‑150 (MQ=255)
                                                                                gccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgcg                                                                    >  1:5308500/1‑150 (MQ=255)
                                                                                  cTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGt                                                                  >  2:3801571/1‑150 (MQ=255)
                                                                                  cTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGt                                                                  >  1:2504581/1‑150 (MQ=255)
                                                                                    tCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCa                                                                <  1:4397683/150‑1 (MQ=255)
                                                                                     cGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAg                                                               >  1:2122812/1‑150 (MQ=255)
                                                                                     cGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAg                                                               >  2:2483459/1‑150 (MQ=255)
                                                                                      ggTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGt                                                              >  1:382362/1‑150 (MQ=255)
                                                                                      ggTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGt                                                              <  2:2235408/150‑1 (MQ=255)
                                                                                      ggTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGt                                                              <  2:2122825/150‑1 (MQ=255)
                                                                                         tgttACTTCCTGACGCTTATCCGGTACCAGGCAGCAAAAATGTGGCATCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAt                                                           >  1:1995939/1‑150 (MQ=255)
                                                                                          gttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTCTCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAtg                                                          >  2:3683573/1‑150 (MQ=255)
                                                                                           ttACTTCCTGACGCTTAACCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGGCGCGGGCCGTAAtgt                                                         >  2:494479/1‑150 (MQ=255)
                                                                                               ttCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACg                                                     >  2:4581006/1‑150 (MQ=255)
                                                                                               ttCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACg                                                     <  1:1009545/150‑1 (MQ=255)
                                                                                                tCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACgg                                                    <  2:1287015/150‑1 (MQ=255)
                                                                                                 ccTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGc                                                   >  2:1154777/1‑150 (MQ=255)
                                                                                                  cagACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCATCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCCGTAATGTGACGGCg                                                  >  2:1891185/3‑150 (MQ=255)
                                                                                                  cTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCg                                                  <  2:4326254/150‑1 (MQ=255)
                                                                                                   tGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGa                                                 >  1:4975875/1‑150 (MQ=255)
                                                                                                    gACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGAt                                                >  2:218782/1‑150 (MQ=255)
                                                                                                      cGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCt                                              <  1:932990/150‑1 (MQ=255)
                                                                                                       gCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCtt                                             <  2:1219484/150‑1 (MQ=255)
                                                                                                        cTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTc                                            >  1:1775485/1‑150 (MQ=255)
                                                                                                        cTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTc                                            >  1:3162994/1‑150 (MQ=255)
                                                                                                        cTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTc                                            <  2:1790361/150‑1 (MQ=255)
                                                                                                         ttttCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGANGGCGATCTTCa                                           <  1:2767292/150‑1 (MQ=255)
                                                                                                         ttttCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCa                                           <  2:2588017/150‑1 (MQ=255)
                                                                                                              cGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTaa                                      >  2:629199/1‑150 (MQ=255)
                                                                                                                 tACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATg                                   >  1:105525/1‑150 (MQ=255)
                                                                                                                 tACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATg                                   <  2:2870796/150‑1 (MQ=255)
                                                                                                                  aCCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGc                                  <  1:3620367/150‑1 (MQ=255)
                                                                                                                   ccAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCa                                 <  2:3157144/150‑1 (MQ=255)
                                                                                                                    cAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCAc                                <  2:695379/150‑1 (MQ=255)
                                                                                                                    cAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCAc                                >  2:3952413/1‑150 (MQ=255)
                                                                                                                    cAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCAc                                >  2:807637/1‑150 (MQ=255)
                                                                                                                    cAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCAc                                >  1:3433196/1‑150 (MQ=255)
                                                                                                                     cggCAGCAAACAGGTGGCCTCGTCTCCACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCCTGCGGGTATCCAGCGTCTGACGCAGGAAGCGCACGTCGCGGTCAGTACTGTGACGGCGATCTTCACGTAAATGCACg                               <  2:4825315/149‑1 (MQ=255)
                                                                                                                     aGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACg                               >  1:2540730/1‑150 (MQ=255)
                                                                                                                     aGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACg                               >  2:4748630/1‑150 (MQ=255)
                                                                                                                     aGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACg                               >  2:1873747/1‑150 (MQ=255)
                                                                                                                         agcaAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTaa                           >  1:223944/1‑150 (MQ=255)
                                                                                                                          gcaAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAAt                          <  1:782926/150‑1 (MQ=255)
                                                                                                                            aaaaaTGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGc                        >  1:738156/1‑150 (MQ=255)
                                                                                                                              aaaTGTCGCTTCGTCTCAACGGCTATCGCCAGCATCTCTACGGTCACCGCCATCTCCAGATTCATGCGGGTAACCATCGTCTGACGCATGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCg                      >  2:532370/1‑150 (MQ=255)
                                                                                                                               aaTGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGt                     <  1:306010/150‑1 (MQ=255)
                                                                                                                               aaTGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGt                     >  1:5045542/1‑150 (MQ=255)
                                                                                                                               aaTGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGt                     >  1:3755239/1‑150 (MQ=255)
                                                                                                                                 tgtgGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTcc                   <  1:619324/150‑1 (MQ=255)
                                                                                                                                 tgtgGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGAGTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTcc                   <  1:1443273/150‑1 (MQ=255)
                                                                                                                                 tgtgGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTcc                   <  1:4190443/150‑1 (MQ=255)
                                                                                                                                      cTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTcc              <  1:2997319/150‑1 (MQ=255)
                                                                                                                                       ttCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTccc             >  1:1212225/1‑150 (MQ=255)
                                                                                                                                         cGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGc           >  2:1426391/1‑150 (MQ=255)
                                                                                                                                            ctcAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTg        >  2:3211832/1‑150 (MQ=255)
                                                                                                                                               aaCGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTc     >  2:3978951/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGC  >  CP009273/2694694‑2694989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑