Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,743,438 C→A 100% intergenic (+19/+36) yfjD → / ← grpE UPF0053 family inner membrane protein/heat shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,743,4380CA99.0% 938.5 / 0.3 288intergenic (+19/+36)yfjD/grpEUPF0053 family inner membrane protein/heat shock protein
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (121/164);  total (123/165)
Fisher's exact test for biased strand distribution p-value = 5.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 288 total aligned reads displayed.

ATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGA  >  CP009273/2743291‑2743584
                                                                                                                                                   |                                                                                                                                                  
aTCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGc                                                                                                                                                  >  1:1258508/1‑150 (MQ=255)
 tCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCa                                                                                                                                                 >  2:4382177/1‑150 (MQ=255)
  cccTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCAt                                                                                                                                                <  2:4294806/150‑1 (MQ=255)
   ccggTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTCCCTGTGAAACCGCTGCGCGAGAGTGTGGCGTAGTAACGAAAACGGCCCGGCATTAGCATg                                                                                                                                               <  2:2430188/147‑1 (MQ=255)
    cTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGc                                                                                                                                              >  1:3880664/1‑150 (MQ=255)
      gTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAg                                                                                                                                            >  1:582951/1‑150 (MQ=255)
          cAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGcc                                                                                                                                        >  1:539706/1‑150 (MQ=255)
          cAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGcc                                                                                                                                        <  2:3981417/150‑1 (MQ=255)
           aGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCg                                                                                                                                       >  2:2259493/1‑150 (MQ=255)
           aGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCg                                                                                                                                       >  1:1643174/1‑150 (MQ=255)
              cACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGa                                                                                                                                    >  1:1060935/1‑150 (MQ=255)
              cACCCGCGTGCGTATGGGCGAGAACGATATCGATATTCTCGACGTACAGGACATTTTGATAAAGCTGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGGGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGa                                                                                                                                    <  1:2021168/150‑1 (MQ=255)
                 ccGCGTGCGTCTTGGCGAGTACGATATCGATATTCTCGCCGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTTTGAAACCGCTGCGCGTGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAatt                                                                                                                                 <  2:4595954/150‑1 (MQ=255)
                   gcgtgcgtAGTGGCGAGTACGAGATCGATATTCCCGACGGACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAattat                                                                                                                               <  2:3540116/150‑1 (MQ=255)
                     gtgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAAttatta                                                                                                                             >  1:2655637/1‑150 (MQ=255)
                     gtgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAAttatta                                                                                                                             >  1:3480383/1‑150 (MQ=255)
                      tgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTAc                                                                                                                            <  2:4372840/150‑1 (MQ=255)
                      tgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTAc                                                                                                                            <  2:4448254/150‑1 (MQ=255)
                          ttttGGCGAGTCCTAAATCGATATTCTCGACTTACAGGAAAATTTTATTACGCTGTTAAATGTTTTTCCTGTTAAACCGCTGCGCGAGTGTGTGGCGGAGAAACGAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGaaa                                                                                                                        <  1:2326142/148‑1 (MQ=255)
                           tttGGCTAGTACGTTATCGTTTTTCTCGTCGTACAGGAAAATATTTTTATGCTGGTAAAAGTTTTTCGTGTTAAACCGCTGCGCGAGAGTGTGGCGTTGTAACTAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAg                                                                                                                       <  1:134253/149‑1 (MQ=255)
                             tGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGAGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCa                                                                                                                     <  2:2680702/150‑1 (MQ=255)
                             tGGCGAGTACGATATCGATAGACTCGACGTACAGGACACTATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCa                                                                                                                     <  2:3053038/150‑1 (MQ=255)
                               gCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTTAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGa                                                                                                                   <  1:1314262/150‑1 (MQ=255)
                                  aGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAAt                                                                                                                >  2:625494/1‑150 (MQ=255)
                                      cgatatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAg                                                                                                            >  1:1832012/1‑150 (MQ=255)
                                       gatatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGc                                                                                                           >  2:872156/1‑150 (MQ=255)
                                           tcgttatTCTCGACGTACAGGACAATATGATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCtttt                                                                                                       <  2:1759936/150‑1 (MQ=255)
                                           tcgatatTCTCGACGTACTTTAAAATATTATTAAGCTGTTAAAATTTTTTCCTGTGAAACCTCTGCGCGAGTGTGTGGCGTATTAACTAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCtttt                                                                                                       <  1:3722864/150‑1 (MQ=255)
                                            cgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTg                                                                                                      >  1:1633368/1‑150 (MQ=255)
                                               tatTCTCGACGTACAGGACAATATGATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCtt                                                                                                   <  1:2543476/150‑1 (MQ=255)
                                                atTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCttt                                                                                                  <  1:2066174/150‑1 (MQ=255)
                                                 ttCTCTACGTACAGGACAATATGATTAAGCAGGTAAAATTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTc                                                                                                 <  1:2840395/150‑1 (MQ=255)
                                                  tctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCg                                                                                                <  2:4730263/150‑1 (MQ=255)
                                                    tcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCt                                                                                              >  1:779609/1‑150 (MQ=255)
                                                     cGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTa                                                                                             >  2:3696992/1‑150 (MQ=255)
                                                      gACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTAc                                                                                            <  2:2995523/150‑1 (MQ=255)
                                                       aCGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACa                                                                                           <  1:4204698/150‑1 (MQ=255)
                                                            cAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAc                                                                                      >  2:1557449/1‑150 (MQ=255)
                                                             aGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAccc                                                                                     >  2:5202834/1‑150 (MQ=255)
                                                             aGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAcc                                                                                     <  2:2055669/150‑1 (MQ=255)
                                                             aGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAcc                                                                                     <  2:2198368/150‑1 (MQ=255)
                                                                aCAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATc                                                                                  <  1:3956255/150‑1 (MQ=255)
                                                                 cAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcg                                                                                 >  1:2287484/1‑150 (MQ=255)
                                                                 aaaTATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGTGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcg                                                                                 <  2:1418746/149‑1 (MQ=255)
                                                                    tatGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgccg                                                                              >  1:2982560/1‑150 (MQ=255)
                                                                         ttAAGCAGGTAAAAGTTTTTCCGGTGAACCCGCTGCGCGAGAGTGTGGCGGAGAAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGa                                                                         <  1:1645087/150‑1 (MQ=255)
                                                                          tAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGaa                                                                        >  1:3144002/1‑150 (MQ=255)
                                                                             gCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCg                                                                     <  1:3410110/150‑1 (MQ=255)
                                                                               agttAAAATTTTTTCCTGTGAAACCGCTGCGCGTGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTa                                                                   <  1:2855594/147‑1 (MQ=255)
                                                                                 gTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACg                                                                 >  2:4973088/1‑150 (MQ=255)
                                                                                  tAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGGATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGa                                                                >  1:5232136/1‑150 (MQ=255)
                                                                                  tAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGa                                                                >  2:4007524/1‑150 (MQ=255)
                                                                                   aaaaGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGAACCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATGATTCCGAAAGCAGAAATAAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAc                                                               <  2:2296767/150‑1 (MQ=255)
                                                                                     aaGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCa                                                             >  2:2208841/1‑150 (MQ=255)
                                                                                          tttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAg                                                        >  2:120830/1‑150 (MQ=255)
                                                                                           ttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGc                                                       >  2:1640968/1‑150 (MQ=255)
                                                                                             ccTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTTCTTTAGCTACAGTAACCATCGCCGCACGAATCGTACGACCCATCAGCGt                                                     >  1:4033586/1‑150 (MQ=255)
                                                                                               tgtgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGtat                                                   >  1:3529126/1‑150 (MQ=255)
                                                                                                gtgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACTAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGtata                                                  <  1:1913382/150‑1 (MQ=255)
                                                                                                gtgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGtata                                                  >  1:1041113/1‑150 (MQ=255)
                                                                                                gtgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGtata                                                  >  2:4559849/1‑150 (MQ=255)
                                                                                                 tgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATGAGCATGCAGGGCCGTGAAGTATTACGAAAGCAGAAAGTAAGCGTTTGCTTTCGCTCCAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATaa                                                 >  2:261980/1‑150 (MQ=255)
                                                                                                 tgAAACCGCGGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATaa                                                 <  1:2410129/150‑1 (MQ=255)
                                                                                                  gAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTCCAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAc                                                >  2:3402458/1‑150 (MQ=255)
                                                                                                  caaaCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAATCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAACATAGCCGCACGAATCGTACGAACATTCAGCGTATAAc                                                >  2:528974/2‑150 (MQ=255)
                                                                                                    aaCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAccc                                              <  2:844816/150‑1 (MQ=255)
                                                                                                    aaCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAccc                                              >  2:4801038/1‑150 (MQ=255)
                                                                                                    aaCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAccc                                              >  2:1314608/1‑150 (MQ=255)
                                                                                                    aaCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAccc                                              <  2:2763192/150‑1 (MQ=255)
                                                                                                     aCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCt                                             >  2:2471192/1‑150 (MQ=255)
                                                                                                      ccGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCtt                                            <  1:4705303/150‑1 (MQ=255)
                                                                                                        gCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCt                                          <  1:1755789/150‑1 (MQ=255)
                                                                                                              cgAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATaa                                    >  2:4166068/1‑150 (MQ=255)
                                                                                                               gagaGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAAt                                   >  1:4268179/1‑150 (MQ=255)
                                                                                                                agagTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATg                                  <  2:4196730/150‑1 (MQ=255)
                                                                                                                   gtgtGGCGGAGTAACGAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGccc                               <  1:3211550/150‑1 (MQ=255)
                                                                                                                   gtgtGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGccc                               <  1:5051349/150‑1 (MQ=255)
                                                                                                                   gtgtGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGccc                               >  2:2820238/1‑150 (MQ=255)
                                                                                                                    tgtgGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCa                              <  1:939297/150‑1 (MQ=255)
                                                                                                                     gtgGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAg                             <  1:3563908/150‑1 (MQ=255)
                                                                                                                      tgGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGt                            >  2:4445188/1‑150 (MQ=255)
                                                                                                                         cGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACg                         <  2:2029382/150‑1 (MQ=255)
                                                                                                                         cGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACg                         >  2:3323318/1‑150 (MQ=255)
                                                                                                                           gAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGtt                       <  1:4403594/150‑1 (MQ=255)
                                                                                                                             gTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAc                     >  1:4445551/1‑150 (MQ=255)
                                                                                                                              tAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAcc                    >  2:1817499/1‑150 (MQ=255)
                                                                                                                              tAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAcc                    >  2:589544/1‑150 (MQ=255)
                                                                                                                               aaCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCt                   <  1:1506685/150‑1 (MQ=255)
                                                                                                                               aaCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCt                   >  2:1437682/1‑150 (MQ=255)
                                                                                                                               aaCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCt                   <  1:3300853/150‑1 (MQ=255)
                                                                                                                               aaCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCt                   <  1:2595751/150‑1 (MQ=255)
                                                                                                                                   aaaaCGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGgcg               >  2:761897/1‑150 (MQ=255)
                                                                                                                                   aaaaCGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGgcg               >  1:45548/1‑150 (MQ=255)
                                                                                                                                        ggCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACg          >  2:1045573/1‑150 (MQ=255)
                                                                                                                                          cccGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtc        >  1:4069386/1‑150 (MQ=255)
                                                                                                                                           ccGGCATTAGCATGCAGGGCCGTGAATTCTTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtca       <  1:2177295/150‑1 (MQ=255)
                                                                                                                                              gCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGACATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatca    >  2:4981112/1‑150 (MQ=255)
                                                                                                                                                aTTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCCTTCAGCGTATAACCCTTCTGCAAAATGCCCAGTACGTTACCTGGCGCAACGTCCTCAGa  >  1:4111760/1‑150 (MQ=255)
                                                                                                                                                aTTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGa  >  1:1166725/1‑150 (MQ=255)
                                                                                                                                                aTTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGa  >  1:4984517/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGA  >  CP009273/2743291‑2743584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑