Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 236,234 A→T 100% V148E (GTG→GAG)  yafV ← putative NAD(P)‑binding C‑N hydrolase family amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273236,2340AT98.3% 948.5 / 3.1 291V148E (GTG→GAG) yafVputative NAD(P)‑binding C‑N hydrolase family amidase
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base T (126/160);  total (129/162)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 293 total aligned reads displayed.

CGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCG  >  CP009273/236088‑236380
                                                                                                                                                  |                                                                                                                                                  
cGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAg                                                                                                                                                 <  2:2144954/150‑1 (MQ=255)
 gCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAgg                                                                                                                                                <  2:3070125/150‑1 (MQ=255)
 gCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACACAgg                                                                                                                                                >  2:3326089/1‑150 (MQ=255)
      cGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAAc                                                                                                                                           >  1:4614575/1‑150 (MQ=255)
       gACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACg                                                                                                                                          <  2:2993699/150‑1 (MQ=255)
           cgATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAg                                                                                                                                      <  2:854548/150‑1 (MQ=255)
            gATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGt                                                                                                                                     <  2:3287411/150‑1 (MQ=255)
            gATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGt                                                                                                                                     >  1:2876321/1‑150 (MQ=255)
             aTTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTc                                                                                                                                    <  2:701902/150‑1 (MQ=255)
             aTTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTc                                                                                                                                    >  2:4745156/1‑150 (MQ=255)
              ttGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCg                                                                                                                                   <  2:4193214/150‑1 (MQ=255)
              ttGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCg                                                                                                                                   <  1:3084532/150‑1 (MQ=255)
                 cATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAg                                                                                                                                <  1:3511329/150‑1 (MQ=255)
                  aTCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGc                                                                                                                               >  1:3434955/1‑150 (MQ=255)
                   tCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGca                                                                                                                              <  1:2426297/150‑1 (MQ=255)
                    cccGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGcac                                                                                                                             <  2:967998/150‑1 (MQ=255)
                    cccGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGcac                                                                                                                             <  1:2688257/150‑1 (MQ=255)
                        ccACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCa                                                                                                                         <  2:3970777/150‑1 (MQ=255)
                          acaTACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGc                                                                                                                       >  2:1016746/1‑150 (MQ=255)
                               cGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCaa                                                                                                                  >  1:4027896/1‑150 (MQ=255)
                                  cTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAAt                                                                                                               <  2:579918/150‑1 (MQ=255)
                                  cTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAAt                                                                                                               >  2:1248218/1‑150 (MQ=255)
                                   tGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATa                                                                                                              <  2:5120391/150‑1 (MQ=255)
                                       tctcAATCGCGCGGGCCGTCAGCAATGCCTGCCTGTGCAGAGAGCGCGGAGCAGGCGAGTTGGCGACGTACAGGTCGATGTCATATTCGTTGAGATTTCGCGACCACTCAGGAAAACGTAAGTCGTAGCACTCCAGCGGCAATATACGcc                                                                                                          <  2:2565737/150‑1 (MQ=255)
                                       tctcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGcc                                                                                                          <  2:3559616/150‑1 (MQ=255)
                                        ctcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCa                                                                                                         >  2:3827637/1‑150 (MQ=255)
                                        ctcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCa                                                                                                         >  1:2338386/1‑150 (MQ=255)
                                         tcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAg                                                                                                        <  2:4291494/150‑1 (MQ=255)
                                         tcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAg                                                                                                        <  2:2269703/150‑1 (MQ=255)
                                           aaTCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGcc                                                                                                      <  2:1918867/150‑1 (MQ=255)
                                           aaTCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGcc                                                                                                      <  1:794381/150‑1 (MQ=255)
                                              cgcgcgGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCcgcg                                                                                                   <  1:4593771/150‑1 (MQ=255)
                                               gcgcgGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCgcgc                                                                                                  >  1:690492/1‑150 (MQ=255)
                                                 gcgGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCa                                                                                                >  2:4665462/1‑150 (MQ=255)
                                                 gcgGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCa                                                                                                >  2:4348942/1‑150 (MQ=255)
                                                 gcgGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCa                                                                                                <  1:4507714/150‑1 (MQ=255)
                                                  cgGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCAt                                                                                               >  1:2137969/1‑150 (MQ=255)
                                                      ccGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCca                                                                                           <  1:1343475/150‑1 (MQ=255)
                                                            gCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCa                                                                                     >  2:4861590/1‑150 (MQ=255)
                                                             cAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCAc                                                                                    >  2:2727261/1‑150 (MQ=255)
                                                             cAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCAc                                                                                    >  1:576690/1‑150 (MQ=255)
                                                              aaTGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACt                                                                                   >  1:91923/1‑150 (MQ=255)
                                                              aaTGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACt                                                                                   <  2:3567571/150‑1 (MQ=255)
                                                               aTGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTc                                                                                  <  2:4687743/150‑1 (MQ=255)
                                                                tgcctgccAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTcg                                                                                 >  1:5126807/1‑150 (MQ=255)
                                                                tgcctgccAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTcg                                                                                 >  1:2016317/1‑150 (MQ=255)
                                                                    tgccAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCa                                                                             <  1:4074573/150‑1 (MQ=255)
                                                                          tGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGccc                                                                       <  1:1599228/150‑1 (MQ=255)
                                                                             agagagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCt                                                                    >  1:4377054/1‑150 (MQ=255)
                                                                             agagagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCt                                                                    <  1:162190/150‑1 (MQ=255)
                                                                             agagagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCt                                                                    >  1:1001018/1‑150 (MQ=255)
                                                                               agagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCttt                                                                  <  2:4791151/150‑1 (MQ=255)
                                                                                  gcgcGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTtata                                                               <  2:4278317/150‑1 (MQ=255)
                                                                                  gcgcGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTtata                                                               <  2:248045/150‑1 (MQ=255)
                                                                                     cgGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATg                                                            >  2:3103608/1‑150 (MQ=255)
                                                                                         gCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGa                                                        <  2:2766537/150‑1 (MQ=255)
                                                                                          cAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCTCGCTTTATAATGTAGAt                                                       <  1:3052081/150‑1 (MQ=255)
                                                                                          cAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGAt                                                       >  2:1212574/1‑150 (MQ=255)
                                                                                          cAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGAt                                                       <  1:2699944/150‑1 (MQ=255)
                                                                                           aGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATg                                                      <  2:1376489/150‑1 (MQ=255)
                                                                                            ggCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGc                                                     <  2:2876321/150‑1 (MQ=255)
                                                                                              ccAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTc                                                   <  2:4052971/150‑1 (MQ=255)
                                                                                              ccAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTc                                                   >  1:1031315/1‑150 (MQ=255)
                                                                                                atttGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCAt                                                 <  2:2050236/148‑1 (MQ=255)
                                                                                                aGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCAt                                                 <  2:2236295/150‑1 (MQ=255)
                                                                                                  ttGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCt                                               <  2:5277983/150‑1 (MQ=255)
                                                                                                      cGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCa                                           >  2:3669044/1‑150 (MQ=255)
                                                                                                      cGACGTACAGGGAGAGGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCa                                           <  2:4770371/150‑1 (MQ=255)
                                                                                                        aCGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATg                                         >  2:2288288/1‑150 (MQ=255)
                                                                                                         cGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGc                                        >  2:1153568/1‑150 (MQ=255)
                                                                                                          gTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCg                                       >  2:1845616/1‑150 (MQ=255)
                                                                                                          gTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCg                                       >  2:3644719/1‑150 (MQ=255)
                                                                                                            aCAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGa                                     <  1:1777523/150‑1 (MQ=255)
                                                                                                                  cGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGAt                               <  1:1479731/150‑1 (MQ=255)
                                                                                                                  cGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGAt                               <  1:3240262/150‑1 (MQ=255)
                                                                                                                  cGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGAt                               <  1:4836040/150‑1 (MQ=255)
                                                                                                                  cGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGAt                               <  2:2270018/150‑1 (MQ=255)
                                                                                                                    aGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGa                             >  2:1430785/1‑150 (MQ=255)
                                                                                                                    aGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGa                             >  1:4692227/1‑150 (MQ=255)
                                                                                                                    aGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGa                             >  1:3692292/1‑150 (MQ=255)
                                                                                                                     ggTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGAc                            >  1:3137381/1‑150 (MQ=255)
                                                                                                                      gTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACg                           >  2:4530970/1‑150 (MQ=255)
                                                                                                                       tCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGc                          >  2:1533536/1‑150 (MQ=255)
                                                                                                                           aaTCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTAt                      >  1:938861/1‑150 (MQ=255)
                                                                                                                           aaTCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTAt                      <  1:3015763/150‑1 (MQ=255)
                                                                                                                             tCGTTGAGAGTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCa                    <  2:1763697/150‑1 (MQ=255)
                                                                                                                              cGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCAt                   >  1:1421437/1‑150 (MQ=255)
                                                                                                                                ttGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATaa                 >  1:5258729/1‑150 (MQ=255)
                                                                                                                                 tGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATaaa                <  1:4227985/150‑1 (MQ=255)
                                                                                                                                  gagaTTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATaaaa               >  2:4125904/1‑150 (MQ=255)
                                                                                                                                    gaTTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATACTGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAAt             <  1:257345/150‑1 (MQ=255)
                                                                                                                                     aTTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATg            >  2:786192/1‑150 (MQ=255)
                                                                                                                                       tGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTa          >  2:5215670/1‑150 (MQ=255)
                                                                                                                                        gcgcGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTAc         <  1:3629327/150‑1 (MQ=255)
                                                                                                                                        gcgcGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTAc         <  2:4979537/150‑1 (MQ=255)
                                                                                                                                        gcgcGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTAc         <  2:3956576/150‑1 (MQ=255)
                                                                                                                                         cgcgACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTAcc        <  1:3858264/150‑1 (MQ=255)
                                                                                                                                            gACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTg     >  1:4086315/1‑150 (MQ=255)
                                                                                                                                             aCCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTgc    >  2:2913450/1‑150 (MQ=255)
                                                                                                                                             aCCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTgc    >  1:1129614/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCG  >  CP009273/236088‑236380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑