Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,808,907 C→A 100% E298* (GAA→TAA)  gshA ← glutamate‑cysteine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,808,9070CA99.1% 1125.5 / ‑0.2 345E298* (GAA→TAA) gshAglutamate‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (173/168);  total (175/170)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 350 total aligned reads displayed.

TGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAA  >  CP009273/2808762‑2809053
                                                                                                                                                 |                                                                                                                                                  
  ctgTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTc                                                                                                                                              >  2:1979189/1‑150 (MQ=255)
      tCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATc                                                                                                                                          >  1:4330491/1‑150 (MQ=255)
      tCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATc                                                                                                                                          >  1:1855131/1‑150 (MQ=255)
      tCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATc                                                                                                                                          <  1:5030936/150‑1 (MQ=255)
       cATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCt                                                                                                                                         <  1:4928944/150‑1 (MQ=255)
       cATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCt                                                                                                                                         <  2:4030355/150‑1 (MQ=255)
        aTCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTg                                                                                                                                        >  2:3913766/1‑150 (MQ=255)
             cacCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAaca                                                                                                                                   >  2:3162096/1‑150 (MQ=255)
              acCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAacac                                                                                                                                  <  2:4783136/150‑1 (MQ=255)
               ccAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACg                                                                                                                                 >  1:1668051/1‑150 (MQ=255)
                 aaTCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGtt                                                                                                                               >  2:2869418/1‑150 (MQ=255)
                  aTCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTg                                                                                                                              >  1:872294/1‑150 (MQ=255)
                  aTCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTg                                                                                                                              <  1:4682336/150‑1 (MQ=255)
                  aTCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTg                                                                                                                              <  1:2931265/150‑1 (MQ=255)
                       cGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGtt                                                                                                                         >  2:248640/1‑150 (MQ=255)
                       cGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGtt                                                                                                                         <  1:4290125/150‑1 (MQ=255)
                         agaACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGa                                                                                                                       >  2:4150331/1‑150 (MQ=255)
                          gaACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGAt                                                                                                                      >  2:3478034/1‑150 (MQ=255)
                             cGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTg                                                                                                                   <  2:2978296/150‑1 (MQ=255)
                              gggTTGATGTCCAGCGAACGCAGTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGc                                                                                                                  >  1:3745604/1‑150 (MQ=255)
                                     tGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCctct                                                                                                           <  1:3513933/150‑1 (MQ=255)
                                       tCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTa                                                                                                         <  1:4021149/150‑1 (MQ=255)
                                        ccAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTAc                                                                                                        <  2:277587/150‑1 (MQ=255)
                                        ccAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTAc                                                                                                        >  2:4655257/1‑150 (MQ=255)
                                          aGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCg                                                                                                      <  1:1419881/150‑1 (MQ=255)
                                             gAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCt                                                                                                   <  2:640718/150‑1 (MQ=255)
                                              aaCGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCtt                                                                                                  >  1:4813368/1‑150 (MQ=255)
                                              aaCGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCtt                                                                                                  >  1:2326332/1‑150 (MQ=255)
                                               acccACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCttt                                                                                                 >  2:3619561/4‑150 (MQ=255)
                                                cGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTtc                                                                                                >  1:788488/1‑150 (MQ=255)
                                                  cACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTtctc                                                                                              >  2:4557400/1‑150 (MQ=255)
                                                   aCTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCa                                                                                             <  1:242569/150‑1 (MQ=255)
                                                   aCTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCa                                                                                             >  1:2061153/1‑150 (MQ=255)
                                                       cAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATAc                                                                                         >  1:3196169/1‑150 (MQ=255)
                                                        aaTATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCATTCTGCACCACGTTGCTGTTGATTTGCTGCCTCTTACCGTCTTTCTCAATAcc                                                                                        >  2:3465572/1‑150 (MQ=255)
                                                         atatatTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCa                                                                                       >  2:5220897/1‑150 (MQ=255)
                                                           atatTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAAt                                                                                     >  2:3771685/1‑150 (MQ=255)
                                                             atTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCt                                                                                   >  2:2658095/1‑150 (MQ=255)
                                                              ttCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCtt                                                                                  >  2:2626178/1‑150 (MQ=255)
                                                                cAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTcg                                                                                >  2:3726047/1‑150 (MQ=255)
                                                                      cgccACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCTGGTAACGCGTTTTGGACGAATCGGCGCGTACATTTATTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCACTAACCGTCTTTCTCAAAACCAATCATCGCGTAct                                                                          >  1:2183026/1‑150 (MQ=255)
                                                                      cgccACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTAct                                                                          >  1:3830297/1‑150 (MQ=255)
                                                                      cgccACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTAct                                                                          >  1:4150725/1‑150 (MQ=255)
                                                                       gccACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTActc                                                                         >  1:735693/1‑150 (MQ=255)
                                                                        ccACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTActct                                                                        >  1:4070306/1‑150 (MQ=255)
                                                                         cACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCtt                                                                       <  1:3550633/150‑1 (MQ=255)
                                                                         cACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCtt                                                                       <  1:2007338/150‑1 (MQ=255)
                                                                         cACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCtt                                                                       >  1:1517411/1‑150 (MQ=255)
                                                                           cGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTCCAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTcc                                                                     >  2:4374161/1‑150 (MQ=255)
                                                                           cGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTcc                                                                     <  1:3946057/150‑1 (MQ=255)
                                                                           cGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTcc                                                                     <  1:4965194/150‑1 (MQ=255)
                                                                           cGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTcc                                                                     <  2:78486/150‑1 (MQ=255)
                                                                               aCAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATg                                                                 <  1:3633754/150‑1 (MQ=255)
                                                                               aCAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATg                                                                 >  2:5116290/1‑150 (MQ=255)
                                                                                 aGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTAGCGTACTCTACCGATGGc                                                               >  1:4993733/1‑150 (MQ=255)
                                                                                  gcgcATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCg                                                              <  1:289092/150‑1 (MQ=255)
                                                                                         aGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTTATTTGAAGACTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGTCGTTTTGAt                                                       >  1:2306082/1‑150 (MQ=255)
                                                                                          gAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGAtt                                                      <  1:248640/150‑1 (MQ=255)
                                                                                          gAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGAtt                                                      <  1:1915120/150‑1 (MQ=255)
                                                                                          gAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGAtt                                                      <  1:164152/150‑1 (MQ=255)
                                                                                             ggCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGcc                                                   <  2:4256093/150‑1 (MQ=255)
                                                                                                  cTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGttt                                              <  2:575685/150‑1 (MQ=255)
                                                                                                     gccgcTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAg                                           <  2:1070399/150‑1 (MQ=255)
                                                                                                      ccgcTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAgg                                          >  1:366373/1‑150 (MQ=255)
                                                                                                       cgcTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGc                                         <  1:1085509/150‑1 (MQ=255)
                                                                                                       cgcTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGc                                         >  2:1240411/1‑150 (MQ=255)
                                                                                                        gcTGCGGGGAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGcc                                        <  1:5197791/150‑1 (MQ=255)
                                                                                                         cTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGccc                                       <  1:3260847/150‑1 (MQ=255)
                                                                                                         cTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGccc                                       <  2:2314817/150‑1 (MQ=255)
                                                                                                            cGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCt                                    <  2:1774677/150‑1 (MQ=255)
                                                                                                                  aCGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTAc                              <  1:5006302/150‑1 (MQ=255)
                                                                                                                   cgcgTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACt                             >  2:2127128/1‑150 (MQ=255)
                                                                                                                   cgcgTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACt                             <  1:3816221/150‑1 (MQ=255)
                                                                                                                    gcgTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTc                            <  1:447814/150‑1 (MQ=255)
                                                                                                                    gcgTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTc                            <  2:1196021/150‑1 (MQ=255)
                                                                                                                     cgTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCg                           >  2:434699/1‑150 (MQ=255)
                                                                                                                     cgTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCg                           <  1:953468/150‑1 (MQ=255)
                                                                                                                      gTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGt                          >  1:2612777/1‑150 (MQ=255)
                                                                                                                         ttGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTaaa                       >  2:1612134/1‑150 (MQ=255)
                                                                                                                         ttGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTaaa                       >  2:4734022/1‑150 (MQ=255)
                                                                                                                          tGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAg                      <  1:1061923/150‑1 (MQ=255)
                                                                                                                           ggACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGa                     >  2:398651/1‑150 (MQ=255)
                                                                                                                            gACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGAt                    <  2:4941491/150‑1 (MQ=255)
                                                                                                                            gACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGAt                    <  2:2349898/150‑1 (MQ=255)
                                                                                                                             aCGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATc                   <  2:2444606/150‑1 (MQ=255)
                                                                                                                                aaTCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGtt                >  1:281401/1‑150 (MQ=255)
                                                                                                                                aaTCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGtt                <  2:1855131/150‑1 (MQ=255)
                                                                                                                                  tCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGa              >  1:284353/1‑150 (MQ=255)
                                                                                                                                    ggCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAg            >  1:3890594/1‑150 (MQ=255)
                                                                                                                                       gcgTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTa         <  2:2467781/150‑1 (MQ=255)
                                                                                                                                        cgTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTaa        >  2:3057919/1‑150 (MQ=255)
                                                                                                                                         gTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAAt       >  2:4976339/1‑150 (MQ=255)
                                                                                                                                         gTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAAt       >  2:901939/1‑150 (MQ=255)
                                                                                                                                         gTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAAt       <  2:4290207/150‑1 (MQ=255)
                                                                                                                                         gTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAAt       <  1:5323050/150‑1 (MQ=255)
                                                                                                                                         gTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAAt       >  2:2230336/1‑150 (MQ=255)
                                                                                                                                          tACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATa      <  2:4226838/150‑1 (MQ=255)
                                                                                                                                            cAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATAcc    <  1:3081855/150‑1 (MQ=255)
                                                                                                                                             agttagttTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCa   <  1:3284787/150‑1 (MQ=255)
                                                                                                                                             agttagttTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCa   >  1:4595733/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAA  >  CP009273/2808762‑2809053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑