Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,836,554 G→T 100% I84I (ATC→ATA)  hycH ← hydrogenase 3 maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,836,5540GT99.0% 996.2 / ‑0.0 305I84I (ATC→ATA) hycHhydrogenase 3 maturation protein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (175/127);  total (177/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 306 total aligned reads displayed.

TCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGGTGGTGACCAAT  >  CP009273/2836407‑2836703
                                                                                                                                                   |                                                                                                                                                     
 ctcctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATg                                                                                                                                                    <  1:3266828/150‑1 (MQ=255)
 ctcctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATg                                                                                                                                                    >  2:555429/1‑150 (MQ=255)
 ctcctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATg                                                                                                                                                    <  2:3926644/150‑1 (MQ=255)
   cctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGAc                                                                                                                                                  <  1:2002576/150‑1 (MQ=255)
      cACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGAt                                                                                                                                               >  2:1039832/1‑150 (MQ=255)
       aCCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGAtc                                                                                                                                              >  1:3021993/1‑150 (MQ=255)
       aCCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGAtc                                                                                                                                              <  2:1396516/150‑1 (MQ=255)
       aCCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGAtc                                                                                                                                              <  2:411003/150‑1 (MQ=255)
        ccATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGAtct                                                                                                                                             >  1:1804561/1‑150 (MQ=255)
        ccATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGAtct                                                                                                                                             >  1:1916675/1‑150 (MQ=255)
        ccATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGAtct                                                                                                                                             <  2:4432071/150‑1 (MQ=255)
         cATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGAtctc                                                                                                                                            >  2:4931246/1‑150 (MQ=255)
          aTCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCa                                                                                                                                           <  2:2977102/150‑1 (MQ=255)
          aTCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCa                                                                                                                                           >  2:4589225/1‑150 (MQ=255)
            cAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCAcc                                                                                                                                         <  1:2135725/150‑1 (MQ=255)
             aaaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCa                                                                                                                                        <  2:1364930/150‑1 (MQ=255)
             aaaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCa                                                                                                                                        <  1:3591804/150‑1 (MQ=255)
              aaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCat                                                                                                                                       >  2:609013/1‑150 (MQ=255)
               aTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCata                                                                                                                                      <  1:3105573/150‑1 (MQ=255)
                tAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCatat                                                                                                                                     >  1:1001854/1‑150 (MQ=255)
                 aGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATAtt                                                                                                                                    >  1:5329142/1‑150 (MQ=255)
                   aTGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTAGCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTc                                                                                                                                  <  2:225665/150‑1 (MQ=255)
                           ttCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGcc                                                                                                                          <  1:3973027/150‑1 (MQ=255)
                             ccTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCtt                                                                                                                        >  1:5185401/1‑150 (MQ=255)
                              cTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTc                                                                                                                       >  2:3057081/1‑150 (MQ=255)
                              cTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTc                                                                                                                       >  1:4106004/1‑150 (MQ=255)
                               tgatgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTcc                                                                                                                      >  2:4861153/1‑150 (MQ=255)
                               tgatgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTcc                                                                                                                      >  2:854295/1‑150 (MQ=255)
                               tgatgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTcc                                                                                                                      <  1:1123387/150‑1 (MQ=255)
                                   gaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAttt                                                                                                                  >  1:550700/1‑150 (MQ=255)
                                   gaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAttt                                                                                                                  >  2:3735394/1‑150 (MQ=255)
                                   gaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAttt                                                                                                                  >  1:4854901/1‑150 (MQ=255)
                                    aaTATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAtttt                                                                                                                 >  1:2209543/1‑150 (MQ=255)
                                     atatCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTg                                                                                                                <  2:1483250/150‑1 (MQ=255)
                                       atCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgcg                                                                                                              >  2:4221685/1‑150 (MQ=255)
                                       atCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgcg                                                                                                              <  1:1690278/150‑1 (MQ=255)
                                       atCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTCCCATTTTgcg                                                                                                              >  1:4192776/1‑150 (MQ=255)
                                                caTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTc                                                                                                     >  1:3347376/1‑150 (MQ=255)
                                                caTACTTAACAGCATTGTACTCCATTCCTGATGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTACCATTTTGCGGCGGGCTTc                                                                                                     >  2:4725814/1‑150 (MQ=255)
                                                  tACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGATTCAc                                                                                                   <  1:1335910/150‑1 (MQ=255)
                                                   aCTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCAct                                                                                                  <  1:4695508/150‑1 (MQ=255)
                                                   aCTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCAct                                                                                                  <  1:5215832/150‑1 (MQ=255)
                                                    cTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActg                                                                                                 <  2:4985855/150‑1 (MQ=255)
                                                       aaCAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActgcct                                                                                              >  2:1160989/1‑150 (MQ=255)
                                                        aCAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActgcctg                                                                                             <  1:4404541/150‑1 (MQ=255)
                                                                 tACTCCATTCCTGCTGCTGCGTTGTTTGCGCTGCCCGGGCTGTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCACTTTGCGGCGGGCTTCACTGCCTGCATCCagag                                                                                    <  2:892630/150‑1 (MQ=255)
                                                                 tACTCCAGTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCagag                                                                                    <  2:1889723/150‑1 (MQ=255)
                                                                   cTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTg                                                                                  >  2:22829/1‑150 (MQ=255)
                                                                   cTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTg                                                                                  >  2:1453311/1‑150 (MQ=255)
                                                                      cATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTATGCGGCGGGCTTCACTGCCTGCCTCCAGAGAGGCa                                                                               >  2:5108941/1‑150 (MQ=255)
                                                                       aTTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCaa                                                                              >  1:711567/1‑150 (MQ=255)
                                                                       aTTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCaa                                                                              <  1:212302/150‑1 (MQ=255)
                                                                          ccTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTCATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATcc                                                                           >  1:2528437/1‑150 (MQ=255)
                                                                            tgctgctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCAt                                                                         <  1:1524680/150‑1 (MQ=255)
                                                                             gctgctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATg                                                                        <  1:2041328/150‑1 (MQ=255)
                                                                             gctgctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATg                                                                        >  2:412473/1‑150 (MQ=255)
                                                                               tgctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGcc                                                                      <  1:4532796/150‑1 (MQ=255)
                                                                               tgctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGcc                                                                      >  2:763287/1‑150 (MQ=255)
                                                                               tgctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGcc                                                                      >  2:3170743/1‑150 (MQ=255)
                                                                                   gcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGat                                                                  <  1:3861742/150‑1 (MQ=255)
                                                                                     gAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGatat                                                                >  2:3593333/1‑150 (MQ=255)
                                                                                      aaGTTTGCGCTGCCCGGGCTTTATCGACTGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATAtt                                                               <  1:1904063/150‑1 (MQ=255)
                                                                                      aaGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATAtt                                                               >  2:4291303/1‑150 (MQ=255)
                                                                                       aGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTc                                                              <  2:1709188/150‑1 (MQ=255)
                                                                                       aGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTc                                                              <  2:2210727/150‑1 (MQ=255)
                                                                                         tttGCGCTGCCCGGGCTTTATCGACTGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCAt                                                            <  2:3321426/150‑1 (MQ=255)
                                                                                              gcTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCAc                                                       <  1:1298310/150‑1 (MQ=255)
                                                                                              gcTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCAc                                                       <  1:4049548/150‑1 (MQ=255)
                                                                                              gcTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCAc                                                       >  1:2869846/1‑150 (MQ=255)
                                                                                                 gCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACggg                                                    <  2:1305498/150‑1 (MQ=255)
                                                                                                    cGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAg                                                 >  2:4368160/1‑150 (MQ=255)
                                                                                                     gggCTTTATCGAATGCGTTGGCCAGCATCTGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTACATTTTGCGGCGGGCTTAACTGCCTGCCTCCAGAGTGGCAATACATGCCAGATATTCATCCCACGGGCAgg                                                >  1:4906417/1‑150 (MQ=255)
                                                                                                      ggCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGt                                               >  2:3328357/1‑150 (MQ=255)
                                                                                                            aTCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAgcgc                                         >  1:2486622/1‑150 (MQ=255)
                                                                                                            aTCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAgcgc                                         >  1:5056563/1‑150 (MQ=255)
                                                                                                            aTCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAgcgc                                         <  2:203546/150‑1 (MQ=255)
                                                                                                              cGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGcgccg                                       >  1:3041557/1‑150 (MQ=255)
                                                                                                                 aTGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCtt                                    >  2:2887765/1‑150 (MQ=255)
                                                                                                                  tGCGTTGGCCAGCATCGGCACCTGGTTTATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCTTTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTc                                   <  2:2434806/150‑1 (MQ=255)
                                                                                                                   gCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTcc                                  <  1:3700132/150‑1 (MQ=255)
                                                                                                                    cGTTGGCCAGCATCGGCACATGGTGAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCCTGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCa                                 <  2:3805811/150‑1 (MQ=255)
                                                                                                                     gTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAg                                >  1:2744725/1‑150 (MQ=255)
                                                                                                                           cAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATc                          <  2:2063125/150‑1 (MQ=255)
                                                                                                                              cATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGAt                       >  1:273624/1‑150 (MQ=255)
                                                                                                                               aTCGTCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATa                      >  1:3456123/1‑150 (MQ=255)
                                                                                                                               aTCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCCATCGATa                      >  2:1107641/1‑150 (MQ=255)
                                                                                                                                 cGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAAc                    <  1:1423537/150‑1 (MQ=255)
                                                                                                                                  ggCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAAcc                   <  2:2100437/150‑1 (MQ=255)
                                                                                                                                    cacaTGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAAcccc                 >  2:3004635/1‑150 (MQ=255)
                                                                                                                                    cacaTGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAAcccc                 >  2:4680476/1‑150 (MQ=255)
                                                                                                                                      caTGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCaa               >  1:2660239/1‑150 (MQ=255)
                                                                                                                                       aTGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAg              >  2:1548611/1‑150 (MQ=255)
                                                                                                                                        tGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAgg             >  1:4636820/1‑150 (MQ=255)
                                                                                                                                        tGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAgg             >  1:779171/1‑150 (MQ=255)
                                                                                                                                          gTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAggtg           >  2:3518964/1‑150 (MQ=255)
                                                                                                                                          gTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAggtg           <  2:3958857/150‑1 (MQ=255)
                                                                                                                                           ttAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAggtgg          <  2:2702766/150‑1 (MQ=255)
                                                                                                                                                gTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGGTGGTGAcc     <  1:2344270/150‑1 (MQ=255)
                                                                                                                                                gTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGGTGGTGAcc     >  1:4432348/1‑150 (MQ=255)
                                                                                                                                                gTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGGTGGTGAcc     <  1:854295/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGGTGGTGACCAAT  >  CP009273/2836407‑2836703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑