Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,846,112 C→A 100% I206I (ATC→ATA)  hypD → hydrogenase maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,846,1120CA99.7% 1044.5 / ‑4.5 325I206I (ATC→ATA) hypDhydrogenase maturation protein
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (163/160);  total (163/162)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 328 total aligned reads displayed.

CTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTATCG  >  CP009273/2845966‑2846258
                                                                                                                                                  |                                                                                                                                                  
   cAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCAcc                                                                                                                                              <  2:5159814/150‑1 (MQ=255)
   cAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCAcc                                                                                                                                              >  2:3287621/1‑150 (MQ=255)
     aCAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGa                                                                                                                                            >  1:46333/1‑150 (MQ=255)
           gAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCta                                                                                                                                      >  2:132299/1‑150 (MQ=255)
            aaaGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCtat                                                                                                                                     <  2:4233132/150‑1 (MQ=255)
            aaaGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCtat                                                                                                                                     <  2:1571713/150‑1 (MQ=255)
             aaGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCtata                                                                                                                                    >  2:912476/1‑150 (MQ=255)
                  cgTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAtttt                                                                                                                               >  1:2255428/1‑150 (MQ=255)
                   gTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTa                                                                                                                              >  1:1624751/1‑150 (MQ=255)
                      aTGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCg                                                                                                                           <  2:1280261/150‑1 (MQ=255)
                         tgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCa                                                                                                                        >  2:4151276/1‑150 (MQ=255)
                         tgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCa                                                                                                                        >  1:1731305/1‑150 (MQ=255)
                             gAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGa                                                                                                                    >  1:1881593/1‑150 (MQ=255)
                               aTTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAtt                                                                                                                  >  1:865070/1‑150 (MQ=255)
                               aTTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAtt                                                                                                                  <  2:1824778/150‑1 (MQ=255)
                                 ttttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAtttt                                                                                                                >  2:23930/1‑150 (MQ=255)
                                 ttttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAtttt                                                                                                                <  2:3931764/150‑1 (MQ=255)
                                 ttttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAtttt                                                                                                                <  2:4071856/150‑1 (MQ=255)
                                 ttttACTTCTTCTGACAGCACATTACGCTTATCCCGTCTTTGCGCAGTTTGCTGGAACAGCCGGATATCGGTTTCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAtttt                                                                                                                <  1:5190663/150‑1 (MQ=255)
                                    tACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                             >  1:3221534/1‑150 (MQ=255)
                                    tACTTCTTCTGCCAGCACAATACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGACGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                             >  1:5330324/1‑150 (MQ=255)
                                      cttcttCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGTGATTTTCATCg                                                                                                           <  2:2769118/150‑1 (MQ=255)
                                      cttcttCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCg                                                                                                           <  1:903496/150‑1 (MQ=255)
                                        tcttctGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTc                                                                                                         <  2:2110064/150‑1 (MQ=255)
                                         cttctGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTcc                                                                                                        <  2:1171230/150‑1 (MQ=255)
                                          ttctGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTcca                                                                                                       <  2:4355905/150‑2 (MQ=255)
                                          ttctGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCg                                                                                                       <  1:2815126/150‑1 (MQ=255)
                                            ctGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCt                                                                                                     <  2:151101/150‑1 (MQ=255)
                                               ccAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTCTAATTTTATCGCCAGCGATTTTCATCGTCCGCtggt                                                                                                  >  2:4109766/1‑150 (MQ=255)
                                               ccAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGCTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggt                                                                                                  >  2:2236537/1‑150 (MQ=255)
                                                cAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggtg                                                                                                 >  2:1051910/1‑150 (MQ=255)
                                                 aGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGTCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggtgg                                                                                                >  1:1807795/1‑150 (MQ=255)
                                                  gCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTggtggt                                                                                               <  2:528766/150‑1 (MQ=255)
                                                  gCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTggtggt                                                                                               <  2:3498756/150‑1 (MQ=255)
                                                   cacaTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGtt                                                                                              <  1:4003212/150‑1 (MQ=255)
                                                     caTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGc                                                                                            <  1:826888/150‑1 (MQ=255)
                                                        tACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTgg                                                                                         >  1:3048794/1‑150 (MQ=255)
                                                         aCGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGa                                                                                        >  1:4888003/1‑150 (MQ=255)
                                                          cGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGAt                                                                                       <  1:2583817/150‑1 (MQ=255)
                                                            cTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTc                                                                                     >  1:2398972/1‑150 (MQ=255)
                                                             ttATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCg                                                                                    <  1:5161849/150‑1 (MQ=255)
                                                              tATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGa                                                                                   <  1:2101040/150‑1 (MQ=255)
                                                                tCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAAc                                                                                 >  1:1113602/1‑150 (MQ=255)
                                                                 cccGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAAcc                                                                                >  2:815577/1‑150 (MQ=255)
                                                                 cccGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAAcc                                                                                >  2:4443138/1‑150 (MQ=255)
                                                                  ccGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAAccc                                                                               <  2:2960728/150‑1 (MQ=255)
                                                                   cGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAAcccc                                                                              >  1:738600/1‑150 (MQ=255)
                                                                    gACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCt                                                                             <  2:62817/150‑1 (MQ=255)
                                                                    gACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCt                                                                             >  1:2514188/1‑150 (MQ=255)
                                                                     aCGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCtt                                                                            <  1:1314428/150‑1 (MQ=255)
                                                                     aCGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCtt                                                                            <  2:4856296/150‑1 (MQ=255)
                                                                       gTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGa                                                                          >  2:1957356/1‑150 (MQ=255)
                                                                        ttGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGAt                                                                         <  1:1378258/150‑1 (MQ=255)
                                                                            gcAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctac                                                                     <  2:3626702/150‑1 (MQ=255)
                                                                             cAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctact                                                                    >  1:3692150/1‑150 (MQ=255)
                                                                             cAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctact                                                                    <  1:2605349/150‑1 (MQ=255)
                                                                              aGTTTGCTGGAACAGCCGGATAACTGTAACGATGCGTTCCTCGAGCCGGGTCACGTCAGAATGGAAATAGGCACCGACGCCTATAAAAATATCGCCAGCGAAATTCAACGACCGCTCGTGGTTGCTGGATACGAACCCCTTGATctaatc                                                                   >  1:3392533/1‑147 (MQ=255)
                                                                              aGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctacta                                                                   >  2:2480189/1‑150 (MQ=255)
                                                                               gTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCtactac                                                                  <  1:1444566/150‑1 (MQ=255)
                                                                                 ttGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACaa                                                                >  1:1957164/1‑150 (MQ=255)
                                                                                  tGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAg                                                               >  2:3730151/1‑150 (MQ=255)
                                                                                  tGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAg                                                               <  1:2645151/150‑1 (MQ=255)
                                                                                   gCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAgg                                                              <  2:1126575/150‑1 (MQ=255)
                                                                                       gAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTg                                                          <  1:4292508/150‑1 (MQ=255)
                                                                                        aaCAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTgg                                                         >  1:2440317/1‑150 (MQ=255)
                                                                                          cAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTc                                                       >  2:4187572/1‑150 (MQ=255)
                                                                                           aGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCa                                                      <  1:1229033/150‑1 (MQ=255)
                                                                                            gCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCAt                                                     >  2:2580048/1‑150 (MQ=255)
                                                                                             ccGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATg                                                    >  2:2146098/1‑150 (MQ=255)
                                                                                               ggATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCt                                                  >  1:102858/1‑150 (MQ=255)
                                                                                               ggATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCt                                                  >  1:4430147/1‑150 (MQ=255)
                                                                                                   atcGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTg                                              <  2:5330324/148‑1 (MQ=255)
                                                                                                   aaCGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTg                                              <  1:2034646/150‑1 (MQ=255)
                                                                                                     cGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTgca                                            <  1:4473214/150‑1 (MQ=255)
                                                                                                       gTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTgcagc                                          >  1:4995906/1‑150 (MQ=255)
                                                                                                            gATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGaaa                                     >  1:709086/1‑150 (MQ=255)
                                                                                                            gATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGaaa                                     >  1:3473851/1‑150 (MQ=255)
                                                                                                               gCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATa                                  <  2:3145452/150‑1 (MQ=255)
                                                                                                               gCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATa                                  <  2:3802669/150‑1 (MQ=255)
                                                                                                                 ggtCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGACCCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGc                                <  2:2677142/148‑1 (MQ=255)
                                                                                                                 attCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGc                                >  2:285574/2‑150 (MQ=255)
                                                                                                                      tCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGccc                           <  2:1301416/150‑1 (MQ=255)
                                                                                                                       cgcgCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCca                          <  1:2946847/150‑1 (MQ=255)
                                                                                                                        gcgcCGGGTCACGTCAGTTTGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCcac                         <  1:3863767/150‑1 (MQ=255)
                                                                                                                         cgcCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCcaca                        <  2:5179289/150‑1 (MQ=255)
                                                                                                                          gcCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCTGAAAATAGCGGCCCACAg                       <  2:1881593/150‑1 (MQ=255)
                                                                                                                          gcCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAg                       >  2:2441509/1‑150 (MQ=255)
                                                                                                                            cGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCa                     <  1:3972700/150‑1 (MQ=255)
                                                                                                                            cGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCa                     >  2:4715855/1‑150 (MQ=255)
                                                                                                                               gTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCTATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAgg                  <  1:1582522/150‑1 (MQ=255)
                                                                                                                               gTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATAGTCCTCAGGTGGTAGCTGGATTCGAACCCCTTGATCTACAACAATTCGTGGTCAAGCTGGTGCAGCAGAAAATAGCGGCCCACAGCaacg                  >  2:560554/1‑148 (MQ=255)
                                                                                                                                tCACGTCTGTATGGTTATAGGCACCGACGCATATAATTTTATCGCCAGCGATTTTCTTCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGGGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGt                 <  1:3287621/150‑1 (MQ=255)
                                                                                                                                 cACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTa                >  1:4157830/1‑150 (MQ=255)
                                                                                                                                   cGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTaga              >  1:4807906/1‑150 (MQ=255)
                                                                                                                                    gTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTagag             <  2:46333/150‑1 (MQ=255)
                                                                                                                                     tCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAACATAGCGGCCCACAGCAAGGTAgaga            <  2:5075800/150‑1 (MQ=255)
                                                                                                                                        gTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCTATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATATACTACAAGGCGTGGTCATGCTGGTGCAGCATAAAATAGCGGCCCACAGCAAGGTAGAGAATc         <  1:2733040/150‑1 (MQ=255)
                                                                                                                                          aTGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAg       <  2:3250145/150‑1 (MQ=255)
                                                                                                                                            ggTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACACGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTa     >  2:774063/1‑150 (MQ=255)
                                                                                                                                              ttATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTAtc   >  2:1430699/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTATCG  >  CP009273/2845966‑2846258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑