Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,855,673 C→A 14.0% I295I (ATC→ATA)  ygbJ → putative dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,855,6730CA14.0% 1121.8 / 100.7 357I295I (ATC→ATA) ygbJputative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (158/149);  new base A (29/21);  total (187/170)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 364 total aligned reads displayed.

AAGGATCTTGGTCTGGTTGCCGATACAGCCAAAGCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATC  >  CP009273/2855524‑2855822
                                                                                                                                                     |                                                                                                                                                     
   gATCTTGGTCTGGTTGCCGATACAGCCAAAGCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTACCGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCAct                                                                                                                                                    >  1:4253789/1‑150 (MQ=255)
     tCTTGGTCTGGTTGCCGATACAGCCAAAGCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCActct                                                                                                                                                  <  1:2060215/150‑1 (MQ=255)
     tCTTGGTCTGGTAGCCGATACAGCCAAAGCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATTTGTTCACCAGCGCCAGTATCGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCActct                                                                                                                                                  <  2:3494292/150‑1 (MQ=255)
           tCTGGTTGCCGATACAGCCAAAGCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATAACTCTACCggg                                                                                                                                            >  1:5200748/1‑150 (MQ=255)
              ggTTGCCGATACAGCCAAAGCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGc                                                                                                                                         >  1:1461336/1‑150 (MQ=255)
                    cGATACAGCCAAAGCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATc                                                                                                                                   <  2:1735760/150‑1 (MQ=255)
                     gATACAGCCAAAGCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCa                                                                                                                                  <  2:2205323/150‑1 (MQ=255)
                     gATACAGCCAAAGCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCa                                                                                                                                  <  2:1443780/150‑1 (MQ=255)
                     gATACAGCCAAAGCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCa                                                                                                                                  >  1:67483/1‑150 (MQ=255)
                      aTACAGCCAAAGCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCAt                                                                                                                                 >  2:4054940/1‑150 (MQ=255)
                          aGCCAAAGCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATc                                                                                                                             >  1:4923512/1‑150 (MQ=255)
                              aaaGCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGa                                                                                                                         >  2:458285/1‑150 (MQ=255)
                               aaGCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGAt                                                                                                                        >  1:2347549/1‑150 (MQ=255)
                                 gCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTg                                                                                                                      <  2:5149117/150‑1 (MQ=255)
                                  cccTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTgg                                                                                                                     >  1:137754/1‑150 (MQ=255)
                                       cACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGACAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATAACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTa                                                                                                                >  2:5210894/1‑150 (MQ=255)
                                       cACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTa                                                                                                                <  2:4359594/150‑1 (MQ=255)
                                       cACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTa                                                                                                                <  2:3388/150‑1 (MQ=255)
                                        aCTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATAACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTAt                                                                                                               >  2:539517/1‑150 (MQ=255)
                                         cTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATTTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATc                                                                                                              <  2:566058/150‑1 (MQ=255)
                                                cTGCCATTGGCCTCACCAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCgatg                                                                                                       <  2:2369467/150‑1 (MQ=255)
                                                cTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCgatg                                                                                                       <  1:2309097/150‑1 (MQ=255)
                                                cTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCgatg                                                                                                       >  1:3546279/1‑150 (MQ=255)
                                                 tGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCgatga                                                                                                      >  1:3627064/1‑150 (MQ=255)
                                                  gCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCgatgat                                                                                                     <  1:4339050/150‑1 (MQ=255)
                                                   ccATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGAtt                                                                                                    >  2:49970/1‑150 (MQ=255)
                                                     aTTGGCCTCTACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCTGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGAtttt                                                                                                  <  2:1393216/150‑1 (MQ=255)
                                                     aTTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGAtttt                                                                                                  >  1:2972329/1‑150 (MQ=255)
                                                     aTTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGAtttt                                                                                                  <  2:2544800/150‑1 (MQ=255)
                                                       tGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGAGTTATCGCCGATGATTTTAc                                                                                                >  1:1041669/1‑150 (MQ=255)
                                                          ccTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCgg                                                                                             >  2:1289777/1‑150 (MQ=255)
                                                           cTCAACAGCATTGAATATGCTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATAACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGgc                                                                                            >  1:789670/1‑150 (MQ=255)
                                                             cAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGgcgc                                                                                          >  2:2938979/1‑150 (MQ=255)
                                                              aaCAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGcgcg                                                                                         <  2:2768187/150‑1 (MQ=255)
                                                                 aGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATAACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACg                                                                                      >  2:3625338/1‑150 (MQ=255)
                                                                  gCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACgg                                                                                     >  1:1067107/1‑150 (MQ=255)
                                                                    aTTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATAACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGat                                                                                   <  1:28670/150‑1 (MQ=255)
                                                                     ttGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGata                                                                                  <  1:922634/150‑1 (MQ=255)
                                                                        aaTATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCg                                                                               >  1:1265351/1‑150 (MQ=255)
                                                                         atatGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGc                                                                              >  2:2682117/1‑150 (MQ=255)
                                                                         atatGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGc                                                                              >  1:966276/1‑150 (MQ=255)
                                                                         atatGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGc                                                                              >  2:3224174/1‑150 (MQ=255)
                                                                           atGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCa                                                                            >  2:1924598/1‑150 (MQ=255)
                                                                            tGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAg                                                                           <  2:2280518/150‑1 (MQ=255)
                                                                            tGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAg                                                                           >  1:3361255/1‑150 (MQ=255)
                                                                            tGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATAACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAg                                                                           <  2:4931931/150‑1 (MQ=255)
                                                                             gTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGt                                                                          >  2:1641875/1‑150 (MQ=255)
                                                                                cACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGtttt                                                                       <  1:1542840/150‑1 (MQ=255)
                                                                                cACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGtttt                                                                       >  2:2518859/1‑150 (MQ=255)
                                                                                cACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGtttt                                                                       >  2:4680621/1‑150 (MQ=255)
                                                                                cACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTAACGCCGATGATTTTACCGGCGCGACGGATATCGCCAGtttt                                                                       >  2:3544125/1‑150 (MQ=255)
                                                                                   cAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTAACGGCGCGACGGATATCGCCAGTTTTCtg                                                                    >  1:4015212/1‑150 (MQ=255)
                                                                                   cAGCGCCAGTAACGCGGGTGACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCtg                                                                    <  2:3117672/150‑1 (MQ=255)
                                                                                     gcgcCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCtggt                                                                  <  1:1717433/150‑1 (MQ=255)
                                                                                      cgcCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCtggtg                                                                 <  1:1210705/150‑1 (MQ=255)
                                                                                          aGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGaaa                                                             <  2:1214236/150‑1 (MQ=255)
                                                                                            tAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAAc                                                           <  2:4897444/150‑1 (MQ=255)
                                                                                            tAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATAACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAAc                                                           >  1:2105892/1‑150 (MQ=255)
                                                                                             aaCGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATAACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACg                                                          >  2:4152784/1‑150 (MQ=255)
                                                                                             aaCGCGGGTTACGGGAAAGAAGAAGATAGCGCATTTATCAAGATTGTCTCTGGCATAACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGAAGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACg                                                          >  2:659784/1‑150 (MQ=255)
                                                                                              aCGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACgg                                                         >  2:3981056/1‑150 (MQ=255)
                                                                                              aCGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACgg                                                         <  2:830080/150‑1 (MQ=255)
                                                                                                  gggTTACGTGAAAGAAGAAGATAGCGCAGTTATCAAGATTTTCTCTGGAATCACTCGACCGGGAGCGAAATCATTATCAATATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTa                                                     <  1:1829812/150‑1 (MQ=255)
                                                                                                  gggTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTa                                                     <  1:1919812/150‑1 (MQ=255)
                                                                                                       aCGGGAAAGAAGACTATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGTATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAAc                                                <  1:4870972/150‑1 (MQ=255)
                                                                                                        cGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACg                                               <  1:4136614/150‑1 (MQ=255)
                                                                                                         gggAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACgg                                              >  2:2802095/1‑150 (MQ=255)
                                                                                                           gAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTa                                            <  1:1877756/150‑1 (MQ=255)
                                                                                                            aaaGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATAACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTAc                                           <  1:3443309/150‑1 (MQ=255)
                                                                                                                aagaCGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAAt                                       >  1:5092366/1‑150 (MQ=255)
                                                                                                                aagaCGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAAt                                       >  2:1518686/1‑150 (MQ=255)
                                                                                                                 agaCGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGAGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAAtt                                      >  2:2002568/1‑150 (MQ=255)
                                                                                                                  gaCGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTa                                     >  1:3729727/1‑150 (MQ=255)
                                                                                                                    cGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAAc                                   >  1:3253128/1‑150 (MQ=255)
                                                                                                                    cGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAAc                                   <  1:534682/150‑1 (MQ=255)
                                                                                                                         gcgcAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGgtgt                              <  1:2596918/150‑1 (MQ=255)
                                                                                                                          cgcAGTTATCAAGATTTTCTCTGGCTTCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGtt                             <  2:1800759/150‑1 (MQ=255)
                                                                                                                           gcAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTc                            <  1:458285/150‑1 (MQ=255)
                                                                                                                            cAGTTATCAAGATTTTCTCTGGCATAACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTcc                           >  1:2954164/1‑150 (MQ=255)
                                                                                                                            cAGTTATCAAGATTTTCTCTGGCATAACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTcc                           >  1:4633311/1‑150 (MQ=255)
                                                                                                                             aGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCa                          <  2:1116763/150‑1 (MQ=255)
                                                                                                                              gTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCaa                         >  2:5041990/1‑150 (MQ=255)
                                                                                                                              gTTATCAAGATTTTCTCTGGCATAACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCaa                         >  2:3437375/1‑150 (MQ=255)
                                                                                                                              gTTATCAAGATTTTCTCTGGCATAACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCaa                         >  2:762121/1‑150 (MQ=255)
                                                                                                                               ttATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAAc                        >  1:3599719/1‑150 (MQ=255)
                                                                                                                               ttATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATAGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTACAAc                        >  1:3506357/1‑150 (MQ=255)
                                                                                                                                 aTCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAg                      <  2:1040527/150‑1 (MQ=255)
                                                                                                                                 aTCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAg                      <  2:240525/150‑1 (MQ=255)
                                                                                                                                 aTCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAg                      >  1:2548130/1‑150 (MQ=255)
                                                                                                                                   cAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAGGt                    >  2:3270532/1‑150 (MQ=255)
                                                                                                                                   cAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAGGt                    <  2:886927/150‑1 (MQ=255)
                                                                                                                                    aaGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAGGTa                   <  1:4414352/150‑1 (MQ=255)
                                                                                                                                    aaGATTTTCTCTGGCATAACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAGGTa                   <  2:3819975/150‑1 (MQ=255)
                                                                                                                                     aGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAAGTAACGGTGTTCCAACAGGTaa                  >  2:3165309/1‑150 (MQ=255)
                                                                                                                                         tttCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATg              <  1:524530/150‑1 (MQ=255)
                                                                                                                                         tttCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATg              <  1:3108319/150‑1 (MQ=255)
                                                                                                                                                ggCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAg       <  1:4979608/150‑1 (MQ=255)
                                                                                                                                                 gCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGc      <  1:594175/150‑1 (MQ=255)
                                                                                                                                                  cATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCa     >  1:2440270/1‑150 (MQ=255)
                                                                                                                                                     cACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATc  >  1:3814992/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGGATCTTGGTCTGGTTGCCGATACAGCCAAAGCCCTGCACTTCCCGCTGCCATTGGCCTCAACAGCATTGAATATGTTCACCAGCGCCAGTAACGCGGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATC  >  CP009273/2855524‑2855822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑