Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,869,780 G→A 100% intergenic (‑92/‑160) cysD ← / → iap sulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,869,7800GA98.9% 1205.4 / 1.1 369intergenic (‑92/‑160)cysD/iapsulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base A (199/166);  total (202/167)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 369 total aligned reads displayed.

ATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTAT  >  CP009273/2869633‑2869926
                                                                                                                                                   |                                                                                                                                                  
atGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAag                                                                                                                                                  <  1:1925327/150‑1 (MQ=255)
 tgtgGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAagt                                                                                                                                                 <  1:4424274/150‑1 (MQ=255)
     gATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTACGTCGTATTTGATCCATCACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAAGAAGTAGtt                                                                                                                                             >  2:1051855/1‑150 (MQ=255)
       tGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTcc                                                                                                                                           <  2:954024/150‑1 (MQ=255)
         cTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCtt                                                                                                                                         >  2:213177/1‑150 (MQ=255)
            tCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAAc                                                                                                                                      >  1:4073699/1‑150 (MQ=255)
            tCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAAc                                                                                                                                      <  1:3657006/150‑1 (MQ=255)
                 cTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAAt                                                                                                                                 >  2:4956375/1‑150 (MQ=255)
                 cTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAAt                                                                                                                                 >  2:3590733/1‑150 (MQ=255)
                  tCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCATTTGCAATACCGCTATTTTCTTTCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATa                                                                                                                                <  2:4514288/150‑1 (MQ=255)
                  tCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGGTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATa                                                                                                                                <  2:4346357/150‑1 (MQ=255)
                   ccAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATaa                                                                                                                               >  2:3082905/1‑150 (MQ=255)
                     aGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACg                                                                                                                             >  2:4736598/1‑150 (MQ=255)
                         gCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGAttt                                                                                                                         >  1:1392930/1‑150 (MQ=255)
                           cGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTgg                                                                                                                       >  2:120684/1‑150 (MQ=255)
                            gCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGc                                                                                                                      <  1:732176/150‑1 (MQ=255)
                              aGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCaa                                                                                                                    >  1:4604039/1‑150 (MQ=255)
                                gTGAGTAAGTCGTATTTGATCCATAACCGGTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAg                                                                                                                  <  1:101322/150‑1 (MQ=255)
                                   aGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTa                                                                                                               >  1:2500013/1‑150 (MQ=255)
                                   aGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTa                                                                                                               >  1:674565/1‑150 (MQ=255)
                                    gTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTaa                                                                                                              <  2:3558679/150‑1 (MQ=255)
                                      aaGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAata                                                                                                            >  2:527389/1‑150 (MQ=255)
                                       aGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAatat                                                                                                           <  2:1688004/150‑1 (MQ=255)
                                        gtagTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATc                                                                                                          <  1:3560052/147‑1 (MQ=255)
                                           gTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCaaa                                                                                                       >  1:5219925/1‑150 (MQ=255)
                                           gTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCaaa                                                                                                       >  1:4099862/1‑150 (MQ=255)
                                            tATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCaaaa                                                                                                      >  2:2654038/1‑150 (MQ=255)
                                                  aTCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCt                                                                                                >  2:4618477/1‑150 (MQ=255)
                                                   tCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCtt                                                                                               <  2:2809533/150‑1 (MQ=255)
                                                    ccATAACCGTTCCTTTGCAATACCGCAATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATC‑AAAAGTGCTTaa                                                                                             >  1:2635586/1‑150 (MQ=255)
                                                     cATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTaa                                                                                             >  2:3821813/1‑150 (MQ=255)
                                                      aTAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAg                                                                                            >  2:1728006/1‑150 (MQ=255)
                                                      aTAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAg                                                                                            >  1:2850829/1‑150 (MQ=255)
                                                      aTAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAg                                                                                            >  1:2524293/1‑150 (MQ=255)
                                                      aTAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAg                                                                                            >  1:2092844/1‑150 (MQ=255)
                                                       tAACCGTTCCTTTGCAAAACCGCTATAATCTTTCCATCAGAAGTTACGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGAGCATAAgg                                                                                           >  1:1882480/1‑150 (MQ=255)
                                                         aCCGTTCCTTTGCAATACCGCTATGTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGACTGAAATTACCAATTAGAATAAGTAGTTCGTTATCGGAATAACGATTTGGCAAAGCTAATTTCAAAAAGTGCTTAAGGCa                                                                                         <  2:4787967/150‑1 (MQ=255)
                                                          ccGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCAc                                                                                        >  2:4223687/1‑150 (MQ=255)
                                                           cGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCAcc                                                                                       >  1:5282657/1‑150 (MQ=255)
                                                            gTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCg                                                                                      >  2:3617051/1‑150 (MQ=255)
                                                            gTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCg                                                                                      >  1:3283063/1‑150 (MQ=255)
                                                              tCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACGAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGa                                                                                    >  2:753112/1‑150 (MQ=255)
                                                              tCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGa                                                                                    <  2:814642/150‑1 (MQ=255)
                                                               ccTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGAt                                                                                   <  1:3349686/150‑1 (MQ=255)
                                                                     cAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCggg                                                                             >  2:2726583/1‑150 (MQ=255)
                                                                       aTACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGTAATAACGATTTGGCAAAGCTAAGATCAAAAAGTGCTTAAGGCACCGGATTTCgggag                                                                           >  2:2913707/1‑148 (MQ=255)
                                                                          ccGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGttt                                                                        <  2:4043676/150‑1 (MQ=255)
                                                                                 tttCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGa                                                                 >  1:3717946/1‑150 (MQ=255)
                                                                                  ttCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTGTAGGAAGAt                                                                >  2:4939413/1‑150 (MQ=255)
                                                                                    cTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGAttt                                                              <  1:4896320/150‑1 (MQ=255)
                                                                                       gCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGaa                                                           >  2:4185022/1‑150 (MQ=255)
                                                                                         cATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAt                                                         <  2:3418292/150‑1 (MQ=255)
                                                                                          aTCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAtt                                                        >  1:300806/1‑150 (MQ=255)
                                                                                          aTCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAtt                                                        <  1:1113159/150‑1 (MQ=255)
                                                                                          aTCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAtt                                                        >  2:1458883/1‑150 (MQ=255)
                                                                                             aGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGtt                                                     >  2:2610330/1‑150 (MQ=255)
                                                                                              gATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGttt                                                    <  1:3060313/150‑1 (MQ=255)
                                                                                              gATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGttt                                                    <  2:1868551/150‑1 (MQ=255)
                                                                                              gATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGttt                                                    >  1:4286775/1‑150 (MQ=255)
                                                                                                 gTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAg                                                 <  1:213177/150‑1 (MQ=255)
                                                                                                 gTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAg                                                 <  2:3762102/150‑1 (MQ=255)
                                                                                                 gTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAg                                                 <  2:5166883/150‑1 (MQ=255)
                                                                                                      gACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAg                                            >  1:2032982/1‑150 (MQ=255)
                                                                                                        cTATAGGGAGCGTAAGAGAACGAATGAAATTACGAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCg                                          <  1:753112/150‑1 (MQ=255)
                                                                                                         tataGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCgg                                         >  2:2227814/1‑150 (MQ=255)
                                                                                                         tataGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCgg                                         >  1:3819151/1‑150 (MQ=255)
                                                                                                               gagaGTAAGAGAACGAATTAAATTACCAATTAGAATAAGTAGTTCCTTAACGTAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCTGCAGttt                                   <  1:2357011/150‑1 (MQ=255)
                                                                                                               gAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGttt                                   >  1:3998868/1‑150 (MQ=255)
                                                                                                                aGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTc                                  >  1:5082676/1‑150 (MQ=255)
                                                                                                                 gCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCa                                 <  2:2276527/150‑1 (MQ=255)
                                                                                                                   gTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATa                               >  1:1236802/1‑150 (MQ=255)
                                                                                                                    tAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATAc                              <  1:2594786/150‑1 (MQ=255)
                                                                                                                    tAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATAc                              <  1:1742034/150‑1 (MQ=255)
                                                                                                                    tAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATAc                              <  1:5129869/150‑1 (MQ=255)
                                                                                                                     aaGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACt                             >  2:5104540/1‑150 (MQ=255)
                                                                                                                        agaACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATg                          >  2:1542872/1‑150 (MQ=255)
                                                                                                                         gaACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATgg                         >  1:2283743/1‑150 (MQ=255)
                                                                                                                         gaACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATgg                         >  1:3189044/1‑150 (MQ=255)
                                                                                                                             gAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGt                     >  1:1702870/1‑150 (MQ=255)
                                                                                                                             gAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGt                     >  2:1525265/1‑150 (MQ=255)
                                                                                                                              aaTGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTa                    >  1:4343503/1‑150 (MQ=255)
                                                                                                                               aTGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTaa                   >  1:2656031/1‑150 (MQ=255)
                                                                                                                               aTGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTaa                   <  1:1996537/150‑1 (MQ=255)
                                                                                                                               aTGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTaa                   >  2:2752707/1‑150 (MQ=255)
                                                                                                                                  aaaTTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTaaaaa                >  1:3206068/1‑150 (MQ=255)
                                                                                                                                   aaTTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTaaaaaa               >  1:1511728/1‑150 (MQ=255)
                                                                                                                                   aaTTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACAATGGCGGTaaaaaa               >  1:1446683/1‑150 (MQ=255)
                                                                                                                                    aTTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTaaaaaaa              >  2:879912/1‑150 (MQ=255)
                                                                                                                                    aTTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTaaaaaaa              >  2:3910189/1‑150 (MQ=255)
                                                                                                                                      tACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAAtt            <  1:4190418/150‑1 (MQ=255)
                                                                                                                                       aCCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAAttt           <  1:85424/150‑1 (MQ=255)
                                                                                                                                       aCCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAAttt           <  1:5193000/150‑1 (MQ=255)
                                                                                                                                        ccAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTg          <  2:2961175/150‑1 (MQ=255)
                                                                                                                                           aTTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCAt       <  1:3590733/150‑1 (MQ=255)
                                                                                                                                           aTTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCAt       >  1:4758850/1‑150 (MQ=255)
                                                                                                                                            ttAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATg      <  2:674565/150‑1 (MQ=255)
                                                                                                                                             tAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATgg     <  2:3142688/150‑1 (MQ=255)
                                                                                                                                              aGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTACGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGAGGTAAAAAAATTTGCATGGt    >  1:5026680/1‑150 (MQ=255)
                                                                                                                                               gAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTa   >  1:349089/1‑150 (MQ=255)
                                                                                                                                               gAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTa   <  1:291468/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTAT  >  CP009273/2869633‑2869926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑