Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,870,278 C→A 100% I113I (ATC→ATA)  iap → aminopeptidase in alkaline phosphatase isozyme conversion

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,870,2780CA99.7% 924.7 / ‑4.5 283I113I (ATC→ATA) iapaminopeptidase in alkaline phosphatase isozyme conversion
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (172/110);  total (173/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 293 total aligned reads displayed.

GTTTCAGCAAATGGGTTATCGCAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCC  >  CP009273/2870131‑2870424
                                                                                                                                                   |                                                                                                                                                  
gTTTCAGCAAATGGGTTATCGCAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATcataat                                                                                                                                                  <  1:2991119/150‑1 (MQ=255)
gTTTCAGCAAATGGGTTATCGCAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATcataat                                                                                                                                                  <  2:38894/150‑1 (MQ=255)
   tCAGCAAATGGGTTATCGCAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTTCGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTAt                                                                                                                                               <  1:3146829/150‑1 (MQ=255)
     atcAAATGGGTTATCGCAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATgg                                                                                                                                             >  2:971243/3‑150 (MQ=255)
        aaaTGGGTTATCGCAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGgcgc                                                                                                                                          >  1:5328344/1‑150 (MQ=255)
         aaTGGGTTATCGCATTTATATTCGTACATTTAATATTCGTTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAACGCGCCGCAGCAGATCATAATTATGGCGCa                                                                                                                                         <  1:1427324/150‑1 (MQ=255)
            gggTTATCGCAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCt                                                                                                                                      >  1:494798/1‑150 (MQ=255)
              gTTATCGCAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTgg                                                                                                                                    >  2:2140201/1‑150 (MQ=255)
                tATCGCAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGAt                                                                                                                                  >  2:1233355/1‑150 (MQ=255)
                tATCGCAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGAt                                                                                                                                  >  2:1811828/1‑150 (MQ=255)
                tATCGCAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGAt                                                                                                                                  >  1:4517450/1‑150 (MQ=255)
                tATCGCAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGAt                                                                                                                                  >  2:4090009/1‑150 (MQ=255)
                    gCAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACtt                                                                                                                              <  1:3465366/150‑1 (MQ=255)
                     cAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTa                                                                                                                             >  1:1961262/1‑150 (MQ=255)
                     cAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTa                                                                                                                             >  1:2084608/1‑150 (MQ=255)
                          atatTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGccc                                                                                                                        >  2:2517000/1‑150 (MQ=255)
                                gtaaaTTTAATAGTCGGTATATTTATACCGCCCGCGATATTCGTAAGAGCTGGCATACCGTGACGGGAATTACGGTGATTGCCGATCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGa                                                                                                                  <  1:1374687/146‑1 (MQ=255)
                                 gACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAg                                                                                                                 <  1:1737544/150‑1 (MQ=255)
                                  aCATTTAATAGTCTGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACTTGACGGTAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGc                                                                                                                >  2:799525/1‑150 (MQ=255)
                                   cATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCg                                                                                                               >  1:4437235/1‑150 (MQ=255)
                                    aTTTAATATTCGGTATATTTATACCGACCGCGATAATCGTAAGAGCAGGCATAACGTGACGGGAAGTACGGTGATTGACGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATAATTACGCCCCGCTCAGCGa                                                                                                              >  1:336697/1‑150 (MQ=255)
                                    aTTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGa                                                                                                              >  1:4281876/1‑150 (MQ=255)
                                    aTTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGa                                                                                                              >  1:1622191/1‑150 (MQ=255)
                                       tAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGc                                                                                                           >  1:3050617/1‑150 (MQ=255)
                                       tAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGc                                                                                                           >  2:5170556/1‑150 (MQ=255)
                                         aTAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTg                                                                                                         <  1:2524178/150‑1 (MQ=255)
                                         aTAGTCGGGATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTg                                                                                                         >  1:522186/1‑150 (MQ=255)
                                           aGTCGGTATATTTATACCGCCCGCGATAATCGTAAGTGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCGGAGCGATGCTGAc                                                                                                       >  2:2917180/1‑150 (MQ=255)
                                           aGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGAc                                                                                                       <  1:3282369/150‑1 (MQ=255)
                                           aGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGAc                                                                                                       <  1:4819689/150‑1 (MQ=255)
                                           aGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGAc                                                                                                       >  1:1258101/1‑150 (MQ=255)
                                           aGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGAc                                                                                                       >  1:1075829/1‑150 (MQ=255)
                                            gTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCCTCTGGATACTTACGCCCCGCTGAGCGATGCTGACg                                                                                                      >  2:2241488/1‑150 (MQ=255)
                                            gTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACg                                                                                                      >  2:3810118/1‑150 (MQ=255)
                                               ggTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCg                                                                                                   >  2:2417533/1‑150 (MQ=255)
                                                gTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGa                                                                                                  >  1:4975363/1‑150 (MQ=255)
                                                 tataTTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGAt                                                                                                 <  1:3582038/150‑1 (MQ=255)
                                                  atatTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATg                                                                                                <  2:495168/150‑1 (MQ=255)
                                                  atatTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATg                                                                                                >  2:1166146/1‑150 (MQ=255)
                                                  atatTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATg                                                                                                <  2:3514024/150‑1 (MQ=255)
                                                     tttATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCa                                                                                             >  1:1213084/1‑150 (MQ=255)
                                                     tttATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCa                                                                                             >  2:4686699/1‑150 (MQ=255)
                                                         taCCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAAtct                                                                                         >  1:2177264/1‑150 (MQ=255)
                                                         taCCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAAtct                                                                                         <  2:1584043/150‑1 (MQ=255)
                                                             gcccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTcggc                                                                                     >  2:2542859/1‑150 (MQ=255)
                                                               ccgcGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTcggcgg                                                                                   <  2:4465480/150‑1 (MQ=255)
                                                                  cgATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCt                                                                                <  2:2966452/150‑1 (MQ=255)
                                                                   gATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg                                                                               >  2:352192/1‑150 (MQ=255)
                                                                   gATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTg                                                                               >  1:830315/1‑150 (MQ=255)
                                                                    aTAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGa                                                                              >  2:688142/1‑150 (MQ=255)
                                                                    aTAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGa                                                                              >  1:4074252/1‑150 (MQ=255)
                                                                     tAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGAc                                                                             <  2:3601261/150‑1 (MQ=255)
                                                                       aTCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGt                                                                           >  2:1009176/1‑150 (MQ=255)
                                                                       aTCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGt                                                                           >  2:5311370/1‑150 (MQ=255)
                                                                         cGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCACTCTCGGCGGGCTGACGTTa                                                                         >  2:456199/1‑150 (MQ=255)
                                                                            aaGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACaa                                                                      >  2:170193/1‑150 (MQ=255)
                                                                             agagCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAg                                                                     <  1:1153121/150‑1 (MQ=255)
                                                                             agagCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAg                                                                     <  2:2832045/150‑1 (MQ=255)
                                                                             agagCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAg                                                                     >  1:2454525/1‑150 (MQ=255)
                                                                              gagCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAgg                                                                    >  1:651498/1‑150 (MQ=255)
                                                                               agCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGa                                                                   >  1:5301952/1‑150 (MQ=255)
                                                                                gCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGaa                                                                  <  2:3163047/150‑1 (MQ=255)
                                                                                 cTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAAt                                                                 <  1:4560124/150‑1 (MQ=255)
                                                                                 cTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAAt                                                                 >  1:4714761/1‑150 (MQ=255)
                                                                                     cATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgat                                                             >  1:5111270/1‑150 (MQ=255)
                                                                                      aTAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGACTGgatg                                                            >  2:5329473/1‑150 (MQ=255)
                                                                                          cGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATaa                                                        >  2:1293839/1‑150 (MQ=255)
                                                                                            tGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcg                                                      <  1:2170397/150‑1 (MQ=255)
                                                                                             gACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAAGGGATGATAAcgc                                                     >  2:2375912/1‑150 (MQ=255)
                                                                                              aCGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgcc                                                    <  2:4093913/150‑1 (MQ=255)
                                                                                                gggAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccgc                                                  <  1:3235367/150‑1 (MQ=255)
                                                                                                 ggAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCa                                                 <  1:2232815/150‑1 (MQ=255)
                                                                                                    aGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGt                                              >  1:5025629/1‑150 (MQ=255)
                                                                                                     gTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGtt                                             >  2:58371/1‑150 (MQ=255)
                                                                                                      tACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGAAGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGttt                                            >  2:1566382/1‑150 (MQ=255)
                                                                                                       aCGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTa                                           <  1:158871/150‑1 (MQ=255)
                                                                                                         ggTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAgg                                         >  1:4846860/1‑150 (MQ=255)
                                                                                                           tGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGgtg                                       <  1:3546948/150‑1 (MQ=255)
                                                                                                           tGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGgtg                                       <  2:1513259/150‑1 (MQ=255)
                                                                                                            gATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGgtgt                                      <  2:2366671/150‑1 (MQ=255)
                                                                                                                gCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATg                                  <  1:2594051/150‑1 (MQ=255)
                                                                                                                       aTGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGACTGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAAt                           >  2:2296715/1‑150 (MQ=255)
                                                                                                                        tGAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAAtt                          <  1:1097018/150‑1 (MQ=255)
                                                                                                                         gAAGGCAAAGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTg                         <  1:2922481/150‑1 (MQ=255)
                                                                                                                               aaaGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGaa                   >  2:3052097/1‑150 (MQ=255)
                                                                                                                               aaaGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGaa                   >  2:5000877/1‑150 (MQ=255)
                                                                                                                                aaGCGCCGCAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAAc                  >  2:3977227/1‑150 (MQ=255)
                                                                                                                                   cgccgcAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGcc               >  1:2356532/1‑150 (MQ=255)
                                                                                                                                    gccgcAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCt              >  1:1781387/1‑150 (MQ=255)
                                                                                                                                    gccgcAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCt              <  1:4090029/150‑1 (MQ=255)
                                                                                                                                     ccgcAGCAGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTg             <  1:4380082/150‑1 (MQ=255)
                                                                                                                                       gcagcaGATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGaa           >  2:3042745/1‑150 (MQ=255)
                                                                                                                                         agcagATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGaaaa         >  2:1882831/1‑150 (MQ=255)
                                                                                                                                         agcagATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGaaaa         >  2:2624615/1‑150 (MQ=255)
                                                                                                                                            agATCATAATTATGGCGCATCTGTATACTTACGCCCCTCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATa      <  1:1905770/150‑1 (MQ=255)
                                                                                                                                             gATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATAc     >  1:671157/1‑150 (MQ=255)
                                                                                                                                             gATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATAc     >  2:4612391/1‑150 (MQ=255)
                                                                                                                                             gATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATAc     <  1:3795780/150‑1 (MQ=255)
                                                                                                                                             gATCATAATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATAc     >  2:3381848/1‑150 (MQ=255)
                                                                                                                                                cataatTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGcc  >  2:1985655/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTTCAGCAAATGGGTTATCGCAGTGATATTCGGACATTTAATAGTCGGTATATTTATACCGCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCC  >  CP009273/2870131‑2870424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑