Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,832,308 G→C S234T (AGT→ACT)  yicL → EamA family inner membrane putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,832,3080GC98.1% 796.7 / NA 209S234T (AGT→ACT) yicLEamA family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (104/101);  minor base A (4/0);  total (108/101)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

TTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGA  >  CP009273/3832163‑3832451
                                                                                                                                                 |                                                                                                                                               
  aCCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTa                                                                                                                                           >  2:3172468/1‑150 (MQ=255)
    cAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTAcc                                                                                                                                         <  1:306646/150‑1 (MQ=255)
      gtcgtcGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTg                                                                                                                                       <  2:3629423/150‑1 (MQ=255)
          tcgGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAg                                                                                                                                   <  2:905963/150‑1 (MQ=255)
          tcgGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAg                                                                                                                                   <  2:3274337/150‑1 (MQ=255)
           cgacTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAgg                                                                                                                                  >  2:1868870/4‑150 (MQ=255)
             gCTGGAGTATGCTGATTGGCGTTATGTTTATGTTGCCTTTTTATGCCAGACATGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTTTTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGTAg                                                                                                                                <  2:1552290/150‑1 (MQ=255)
             gCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAg                                                                                                                                <  2:1164098/150‑1 (MQ=255)
              cTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCAGACATTTACTCTGTACCTGAAAAGAGc                                                                                                                               >  1:1637786/1‑150 (MQ=255)
               tGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACATTTACTCTGTACCTGAAATGAGca                                                                                                                              >  1:2861562/1‑150 (MQ=255)
               tGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGACGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTACCTGACATTTAATCTGTACCTGAAATGAGca                                                                                                                              >  1:2861809/1‑150 (MQ=255)
                ggAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGcac                                                                                                                             <  2:746013/150‑1 (MQ=255)
                  aGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACaa                                                                                                                           >  2:203171/1‑150 (MQ=255)
                   gTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACaat                                                                                                                          >  2:687671/1‑150 (MQ=255)
                    tATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACaatt                                                                                                                         >  1:445517/1‑150 (MQ=255)
                    tATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACaatt                                                                                                                         <  2:2463956/150‑1 (MQ=255)
                     aTGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACaatta                                                                                                                        <  1:1484952/150‑1 (MQ=255)
                     aTGCTGATTGGCGGTCTGATTCTGTTGCATTTTTATTCCAGACAAGGAAAAAAATTTGTCGTTAACGGCAGTTTTATTCTGGCGTTTTTTTATTTGGTTGTCATTGGTAAGTACCTGACATTTACTCTGTAACTGAAATGAGCACaatta                                                                                                                        >  2:1002808/1‑150 (MQ=255)
                        cTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACaattaatt                                                                                                                     <  2:1818881/150‑1 (MQ=255)
                         tGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTg                                                                                                                    <  2:3553510/150‑1 (MQ=255)
                           aTTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGc                                                                                                                  <  1:2712770/150‑1 (MQ=255)
                              ggCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTTGCGGt                                                                                                               >  1:3041509/1‑150 (MQ=255)
                                 ggTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCa                                                                                                            >  2:2566907/1‑150 (MQ=255)
                                 ggTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCa                                                                                                            <  1:2622720/150‑1 (MQ=255)
                                 ggTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCa                                                                                                            >  2:3400185/1‑150 (MQ=255)
                                 ggTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAAGAGCACAATTAATTTGCGGTCCa                                                                                                            >  1:1595156/1‑150 (MQ=255)
                                  gTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAAATAATTTGCGGTCCaa                                                                                                           >  1:3233723/1‑150 (MQ=255)
                                  gTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAAAGGCAGTTTGATTCTGGCGTTTTTTTATTTTGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCaa                                                                                                           >  2:2397649/1‑150 (MQ=255)
                                       aTTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGcc                                                                                                      <  2:572387/150‑1 (MQ=255)
                                       aTTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGcc                                                                                                      <  2:2173594/150‑1 (MQ=255)
                                         tCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAg                                                                                                    >  2:908069/1‑150 (MQ=255)
                                                 cTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGa                                                                                            >  2:2856232/1‑150 (MQ=255)
                                                  tttttATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCAGACAATTACACTGTACCAGAAAGGAGCACAATTAATTGTCGGTCCAAAATCCAGCATTTTGAg                                                                                           >  1:35773/1‑150 (MQ=255)
                                                   ttttATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGc                                                                                          >  2:729369/1‑150 (MQ=255)
                                                   ttttATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGc                                                                                          <  1:1409818/150‑1 (MQ=255)
                                                       aTGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACCTTTACTCAGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCtgtg                                                                                      >  1:2071206/1‑150 (MQ=255)
                                                         gCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCa                                                                                    <  2:1190461/150‑1 (MQ=255)
                                                          ccAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAg                                                                                   >  2:1995112/1‑150 (MQ=255)
                                                          ccAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAg                                                                                   >  2:84421/1‑150 (MQ=255)
                                                          ccAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAg                                                                                   <  1:797539/150‑1 (MQ=255)
                                                           cAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGa                                                                                  <  1:1025684/150‑1 (MQ=255)
                                                           cAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGa                                                                                  >  1:2174487/1‑150 (MQ=255)
                                                           cAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGa                                                                                  <  1:693374/150‑1 (MQ=255)
                                                            aGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGaa                                                                                 >  2:499859/1‑150 (MQ=255)
                                                              aCAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAAcc                                                                               <  1:2258858/150‑1 (MQ=255)
                                                              aCAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAAcc                                                                               >  2:3379846/1‑150 (MQ=255)
                                                              aCAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAAcc                                                                               <  1:3643692/150‑1 (MQ=255)
                                                              aCAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAAcc                                                                               <  1:3549966/150‑1 (MQ=255)
                                                                aaGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCAt                                                                             >  1:2128348/1‑150 (MQ=255)
                                                                aaGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCAt                                                                             >  1:1820149/1‑150 (MQ=255)
                                                                 aGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCAtt                                                                            >  2:2246399/1‑150 (MQ=255)
                                                                  ggAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTa                                                                           <  1:2744514/150‑1 (MQ=255)
                                                                    aaCAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAg                                                                         <  2:1071576/150‑1 (MQ=255)
                                                                    aaCAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAg                                                                         >  2:1191022/1‑150 (MQ=255)
                                                                      cAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTa                                                                       <  2:1436202/150‑1 (MQ=255)
                                                                      cAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTa                                                                       <  2:1626990/150‑1 (MQ=255)
                                                                       aaaCTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAg                                                                      <  2:1258040/150‑1 (MQ=255)
                                                                       aaaCTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAg                                                                      >  2:3251026/1‑150 (MQ=255)
                                                                       aaaCTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCAGAAAGGAGCACAAATAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAg                                                                      >  1:839315/1‑150 (MQ=255)
                                                                        aaCTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAgc                                                                     <  1:937613/150‑1 (MQ=255)
                                                                          cTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAACCAGCATTTTGAGCTGTGCAGAACCATTAAGTAtcgc                                                                   >  1:2487944/1‑150 (MQ=255)
                                                                               tCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTAc                                                              >  2:2258238/1‑150 (MQ=255)
                                                                                cGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTAct                                                             <  1:1233489/150‑1 (MQ=255)
                                                                                cGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACAATTACTCTGTACCTGAAAGGAGCACAATTAATTTGCGGTCCAAAATCCAGCATTTTGAGCTGTGCAGAACCATTAAGTATCGCGCTAct                                                             >  1:1316772/1‑150 (MQ=255)
                                                                                   tAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTActctc                                                          >  2:777979/1‑150 (MQ=255)
                                                                                    aaCGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTTCTCTGTTCCTGATAGGAGCTCTATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTActctct                                                         <  1:1729585/150‑1 (MQ=255)
                                                                                    aaCGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTActctct                                                         <  2:2618653/150‑1 (MQ=255)
                                                                                      cGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCttt                                                       <  1:1601434/150‑1 (MQ=255)
                                                                                      cGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCttt                                                       <  1:2858460/150‑1 (MQ=255)
                                                                                         cAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgct                                                    <  1:3063656/150‑1 (MQ=255)
                                                                                         cAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgct                                                    <  2:3522470/150‑1 (MQ=255)
                                                                                         cAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgct                                                    >  2:221502/1‑150 (MQ=255)
                                                                                              tGATTCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACAATTAAACTGTACCATAAAAGAGCCCAAATAATTTTCGGTCCAAAAACCAACAATTTTATCTGTGCAAAACCAATAAATAACGCGCTACACTCTttgctgttgc                                               >  1:2239280/1‑150 (MQ=255)
                                                                                               gATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgatgttgat                                              >  2:1957154/1‑148 (MQ=255)
                                                                                                 ttCTGGCGTTTTTTTATTTGGTTGTCATTGGTACGTCCCTGACCTTTACTCTGTACCTGAAAGGAGCACAATTAATTTGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAATAAGAGCGCTACTCTCATTGCTGTTGATgg                                            >  2:1929217/1‑150 (MQ=255)
                                                                                                 ttCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACATTTACTCTGTACCTGAAAAGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTTCATAACCATTAATTAGCGCGCTACTCTCTTTTCTGTTGCTgg                                            >  1:2313024/1‑150 (MQ=255)
                                                                                                   cTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTgggg                                          >  1:3095799/1‑150 (MQ=255)
                                                                                                    tGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGa                                         <  1:687620/150‑1 (MQ=255)
                                                                                                       cGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCa                                      >  1:144543/1‑150 (MQ=255)
                                                                                                          ttttttATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCTCGCTACTCTCTTTGATGTTGCTGGGGATCACGt                                   >  2:1438922/1‑150 (MQ=255)
                                                                                                           tttttATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGtt                                  >  2:2626395/1‑150 (MQ=255)
                                                                                                            ttttATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTc                                 >  1:1286720/1‑150 (MQ=255)
                                                                                                             tttATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTca                                <  1:186157/150‑1 (MQ=255)
                                                                                                              ttATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTcac                               <  2:2950283/150‑1 (MQ=255)
                                                                                                                 tttGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACAtt                            <  1:1759576/150‑1 (MQ=255)
                                                                                                                   tggtggTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTAc                          >  2:1504145/1‑150 (MQ=255)
                                                                                                                         tCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGAct                    <  1:1846315/150‑1 (MQ=255)
                                                                                                                           aTTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActgg                  >  2:2955592/1‑150 (MQ=255)
                                                                                                                           aTTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActgg                  >  1:3725203/1‑150 (MQ=255)
                                                                                                                            ttGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActggc                 >  1:1296131/1‑150 (MQ=255)
                                                                                                                                tACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTggg             <  1:3400185/150‑1 (MQ=255)
                                                                                                                                  cGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGaa           >  1:1271804/1‑150 (MQ=255)
                                                                                                                                   gTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAAc          >  2:2113220/1‑150 (MQ=255)
                                                                                                                                   gTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAAc          >  2:713711/1‑150 (MQ=255)
                                                                                                                                   gTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAAc          >  1:2872101/1‑150 (MQ=255)
                                                                                                                                     cccTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgc        <  2:445517/150‑1 (MQ=255)
                                                                                                                                      ccTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgct       <  2:1490246/150‑1 (MQ=255)
                                                                                                                                      ccTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgct       >  1:2005992/1‑150 (MQ=255)
                                                                                                                                       cTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctg      >  1:348264/1‑150 (MQ=255)
                                                                                                                                       cTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctg      >  1:2687156/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGA  >  CP009273/3832163‑3832451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑