BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,880,731	G→T	D182Y (GAT→TAT)	mnmE →	tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,880,731	0	G	T	100.0%	771.5 / NA	229	D182Y (GAT→TAT)	mnmE	tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (127/102); total (127/102)

Only 100 of 229 total aligned reads displayed.

ATCGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCGATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAATCG  >  CP009273/3880584‑3880877

  cGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATGTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTAttt                                                                                                                                                <  2:3255729/150‑1 (MQ=255)

    aCGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTcc                                                                                                                                              <  2:111336/150‑1 (MQ=255)

     cGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCct                                                                                                                                             <  1:952328/150‑1 (MQ=255)

       ccAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCctct                                                                                                                                           <  1:123000/150‑1 (MQ=255)

        cAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCtctc                                                                                                                                          <  2:1479599/150‑1 (MQ=255)

         aGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTcc                                                                                                                                         >  2:1548241/1‑150 (MQ=255)

             cGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACg                                                                                                                                     >  2:2364021/1‑150 (MQ=255)

             cGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACg                                                                                                                                     <  2:3414798/150‑1 (MQ=255)

             cGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACg                                                                                                                                     >  1:505855/1‑150 (MQ=255)

             cGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACg                                                                                                                                     >  1:3788146/1‑150 (MQ=255)

                aaCAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGaa                                                                                                                                  >  2:1101774/1‑150 (MQ=255)

                   aGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGaaaaa                                                                                                                               >  2:3352383/1‑150 (MQ=255)

                   aGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATGTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGaaaaa                                                                                                                               <  2:357962/150‑1 (MQ=255)

                     gcggcCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATGTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAAtt                                                                                                                             <  2:3016957/150‑1 (MQ=255)

                       ggcCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGa                                                                                                                           <  1:1051100/150‑1 (MQ=255)

                         cccGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAg                                                                                                                         <  2:1383216/150‑1 (MQ=255)

                         cccGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAg                                                                                                                         <  1:3527690/150‑1 (MQ=255)

                          ccGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGc                                                                                                                        <  2:2401745/150‑1 (MQ=255)

                              tCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCaa                                                                                                                    <  2:1457220/150‑2 (MQ=255)

                              tCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAg                                                                                                                    <  1:1950312/150‑1 (MQ=255)

                                  cACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCa                                                                                                                >  2:804426/1‑150 (MQ=255)

                                  cACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCa                                                                                                                >  2:2708605/1‑150 (MQ=255)

                                   aCTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCaa                                                                                                               >  2:758237/1‑150 (MQ=255)

                                    cTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAAt                                                                                                              >  1:2475997/1‑150 (MQ=255)

                                       aaCTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGAc                                                                                                           >  1:1808081/1‑150 (MQ=255)

                                       aaCTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGAc                                                                                                           <  1:3656442/150‑1 (MQ=255)

                                       aaCTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGAc                                                                                                           <  2:3547142/150‑1 (MQ=255)

                                       aaCTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGAc                                                                                                           <  1:1884307/150‑1 (MQ=255)

                                        aCTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACg                                                                                                          <  1:3449457/150‑1 (MQ=255)

                                         cTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGt                                                                                                         >  1:1689707/1‑150 (MQ=255)

                                          tCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGtt                                                                                                        <  1:2285590/150‑1 (MQ=255)

                                           cGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTa                                                                                                       <  2:2332660/150‑1 (MQ=255)

                                             cTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTAtt                                                                                                     >  2:2916812/1‑150 (MQ=255)

                                             cTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTAtt                                                                                                     >  2:2489597/1‑150 (MQ=255)

                                             cTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTAtt                                                                                                     <  2:3210927/150‑1 (MQ=255)

                                             cTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTAtt                                                                                                     >  1:740015/1‑150 (MQ=255)

                                             cNGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTAtt                                                                                                     >  1:1376761/1‑150 (MQ=255)

                                              tGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTg                                                                                                    >  1:941806/1‑150 (MQ=255)

                                                cAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGcc                                                                                                  >  1:3697129/1‑150 (MQ=255)

                                                  aGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGa                                                                                                <  2:1601233/150‑1 (MQ=255)

                                                      gcATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCtt                                                                                            >  1:1729308/1‑150 (MQ=255)

                                                      gcATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCtt                                                                                            <  1:2061713/150‑1 (MQ=255)

                                                           ctcCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGc                                                                                       >  1:585469/1‑150 (MQ=255)

                                                                cACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAgtgc                                                                                  >  1:1210498/1‑150 (MQ=255)

                                                                  cGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAgtgcgt                                                                                <  2:2633295/150‑1 (MQ=255)

                                                                     gTTAATCATCTGGTAGAAGCCCTCACCCACGTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCt                                                                             <  2:206396/150‑1 (MQ=255)

                                                                       tAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGa                                                                           >  1:1875752/1‑150 (MQ=255)

                                                                        aaTCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGaa                                                                          <  2:1240550/150‑1 (MQ=255)

                                                                         atcatcTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAg                                                                         <  2:3107045/150‑1 (MQ=255)

                                                                          tcatcTGGTAGAAGCCCTCACCCACTTGCGCATGTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGc                                                                        <  2:425552/150‑1 (MQ=255)

                                                                            atcTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCAc                                                                      >  1:45208/1‑150 (MQ=255)

                                                                               tGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTc                                                                   >  1:2305068/1‑150 (MQ=255)

                                                                                 gTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAg                                                                 <  1:2639838/150‑1 (MQ=255)

                                                                                  tAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAgg                                                                >  1:3048310/1‑150 (MQ=255)

                                                                                     aaGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTa                                                             >  1:1343653/1‑150 (MQ=255)

                                                                                         ccTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGttt                                                         >  1:2134346/1‑150 (MQ=255)

                                                                                          cTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTttg                                                        <  2:525288/150‑1 (MQ=255)

                                                                                          cTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTttg                                                        <  1:2236747/150‑1 (MQ=255)

                                                                                            cACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTttgtt                                                      <  2:588933/150‑1 (MQ=255)

                                                                                             aCCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTttgttg                                                     <  1:3352383/150‑1 (MQ=255)

                                                                                             aCCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTttgttg                                                     >  1:3486517/1‑150 (MQ=255)

                                                                                              cccACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTgc                                                    >  1:1289174/1‑150 (MQ=255)

                                                                                                  cTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGa                                                >  1:630423/1‑150 (MQ=255)

                                                                                                  cTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGa                                                >  2:1945772/1‑150 (MQ=255)

                                                                                                    tGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAg                                              >  1:1870125/1‑150 (MQ=255)

                                                                                                     gcgcATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAgg                                             <  1:840116/150‑1 (MQ=255)

                                                                                                     gcgcATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAgg                                             <  1:2767611/150‑1 (MQ=255)

                                                                                                       gcATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGa                                           <  1:331300/150‑1 (MQ=255)

                                                                                                       gcATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGa                                           <  2:740015/150‑1 (MQ=255)

                                                                                                        cATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGAt                                          <  1:1589349/150‑1 (MQ=255)

                                                                                                        cATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGAt                                          <  1:900342/150‑1 (MQ=255)

                                                                                                        cATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGAt                                          <  1:3095691/150‑1 (MQ=255)

                                                                                                        cATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGAt                                          <  1:2936304/150‑1 (MQ=255)

                                                                                                         atgtACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATg                                         <  1:3201849/147‑1 (MQ=255)

                                                                                                         aTTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATg                                         <  2:1566529/150‑1 (MQ=255)

                                                                                                         aTTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATg                                         <  2:2265415/150‑1 (MQ=255)

                                                                                                         aTTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATg                                         >  1:2536792/1‑150 (MQ=255)

                                                                                                         aTTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATg                                         >  1:1448037/1‑150 (MQ=255)

                                                                                                         aTTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATg                                         >  1:1774038/1‑150 (MQ=255)

                                                                                                         aTTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATg                                         >  2:2831976/1‑150 (MQ=255)

                                                                                                            tACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGaaa                                      >  2:2147742/1‑150 (MQ=255)

                                                                                                               gTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAgtg                                   >  2:1292374/1‑150 (MQ=255)

                                                                                                                   aaGCGGCAATTGATTTCCCCGATNAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACNTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGa                               >  2:2454487/1‑150 (MQ=255)

                                                                                                                   aaGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGa                               >  2:1870112/1‑150 (MQ=255)

                                                                                                                   aaGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGa                               >  2:3763122/1‑150 (MQ=255)

                                                                                                                      cGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTg                            <  2:645145/150‑1 (MQ=255)

                                                                                                                      cGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTg                            <  2:1327300/150‑1 (MQ=255)

                                                                                                                           aTTGATTTCACCGATGAAGAGATCTATTTCCTCTCCGACGGACAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTCGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGa                       >  2:3554521/1‑150 (MQ=255)

                                                                                                                               aTTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTc                   >  1:203888/1‑150 (MQ=255)

                                                                                                                               aTTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTc                   <  1:1535145/150‑1 (MQ=255)

                                                                                                                               aTTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTc                   <  2:1808081/150‑1 (MQ=255)

                                                                                                                                 ttCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCt                 <  2:2153685/150‑1 (MQ=255)

                                                                                                                                 ttCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCt                 >  2:2412576/1‑150 (MQ=255)

                                                                                                                                 ttCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCt                 >  1:1492651/1‑150 (MQ=255)

                                                                                                                                      cGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGc            >  2:2529152/1‑150 (MQ=255)

                                                                                                                                       gATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGcc           >  1:793065/1‑150 (MQ=255)

                                                                                                                                            agagATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTaa      >  1:2835424/1‑150 (MQ=255)

                                                                                                                                             gagaTCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTaaa     >  2:42618/1‑150 (MQ=255)

                                                                                                                                               gaTCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTTATTGCCGGACGTCCTAACGCCGGTAAATc   <  2:3788146/150‑1 (MQ=255)

                                                                                                                                                aTCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAATCg  >  1:468553/1‑150 (MQ=255)

ATCGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCGATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAATCG  >  CP009273/3880584‑3880877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`