BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,904,553	T→A	P111P (CCA→CCT)	pstS ←	periplasmic phosphate binding protein, high‑affinity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,904,553	0	T	A	100.0%	704.0 / NA	211	P111P (CCA→CCT)	pstS	periplasmic phosphate binding protein, high‑affinity
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (99/112); total (99/112)

Only 100 of 211 total aligned reads displayed.

GCGGCGTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCTGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCACCGGAAGA  >  CP009273/3904406‑3904699

gcggcgTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAgg                                                                                                                                                  >  2:1053384/1‑150 (MQ=255)

 cggcgTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGa                                                                                                                                                 >  1:1359075/1‑150 (MQ=255)

  ggcgTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGaa                                                                                                                                                >  1:2939363/1‑150 (MQ=255)

    cgTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATg                                                                                                                                              >  1:152461/1‑150 (MQ=255)

      tactacAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGtt                                                                                                                                            <  2:2430772/150‑1 (MQ=255)

      tactacAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGtt                                                                                                                                            <  2:447371/150‑1 (MQ=255)

        ctacAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTaa                                                                                                                                          >  1:1166312/1‑150 (MQ=255)

         tacAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAAc                                                                                                                                         <  2:258493/150‑1 (MQ=255)

          acAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAAcc                                                                                                                                        <  1:166651/150‑1 (MQ=255)

           cAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCg                                                                                                                                       >  1:2092355/1‑150 (MQ=255)

            aGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGc                                                                                                                                      <  2:1334491/150‑1 (MQ=255)

            aGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGCTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGc                                                                                                                                      >  2:505659/1‑150 (MQ=255)

              cAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCa                                                                                                                                    <  2:303692/150‑1 (MQ=255)

               aaTGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAg                                                                                                                                   >  1:819138/1‑150 (MQ=255)

               aaTGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGACAGACTTCAGCCCAGGAATGTTAACCGCCAg                                                                                                                                   <  1:2237913/150‑1 (MQ=255)

                  gTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGcac                                                                                                                                <  1:2081711/150‑1 (MQ=255)

                    tttGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGcacca                                                                                                                              <  1:3012349/150‑1 (MQ=255)

                      tgtgAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCAcg                                                                                                                            >  1:440801/1‑150 (MQ=255)

                       gtgAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCAcgc                                                                                                                           >  1:3443265/1‑150 (MQ=255)

                       gtgAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCAcgc                                                                                                                           >  1:2533241/1‑150 (MQ=255)

                        tgAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCAcgcc                                                                                                                          <  2:655039/150‑1 (MQ=255)

                         gAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCAcgccg                                                                                                                         <  2:2706692/150‑1 (MQ=255)

                          aaGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCAcgccgc                                                                                                                        >  2:760274/1‑150 (MQ=255)

                              cAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAAt                                                                                                                    <  1:2030857/150‑1 (MQ=255)

                               aGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATc                                                                                                                   >  1:1436264/1‑150 (MQ=255)

                                 tttCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCAc                                                                                                                 >  2:2144558/1‑150 (MQ=255)

                                  ttCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACg                                                                                                                <  2:785756/150‑1 (MQ=255)

                                   tCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGATTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACgg                                                                                                               <  1:1464520/150‑1 (MQ=255)

                                     aGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTc                                                                                                             <  2:1977120/150‑1 (MQ=255)

                                        cccGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCggg                                                                                                          <  2:2977343/150‑1 (MQ=255)

                                        cccGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCggg                                                                                                          >  1:1819555/1‑150 (MQ=255)

                                         ccGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGa                                                                                                         <  2:3155269/150‑1 (MQ=255)

                                           ggATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAAc                                                                                                       <  2:1359075/150‑1 (MQ=255)

                                            gATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACt                                                                                                      <  1:91429/150‑1 (MQ=255)

                                            gATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACt                                                                                                      <  1:1853619/150‑1 (MQ=255)

                                             aTTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTg                                                                                                     <  2:3108261/150‑1 (MQ=255)

                                              ttCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTgg                                                                                                    >  2:1386612/1‑150 (MQ=255)

                                                cAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGaa                                                                                                  >  1:315486/1‑150 (MQ=255)

                                                cAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGaa                                                                                                  <  2:1113105/150‑1 (MQ=255)

                                                 aGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAAc                                                                                                 >  2:337232/1‑150 (MQ=255)

                                                   tttGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAg                                                                                               <  2:802772/150‑1 (MQ=255)

                                                     tGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGAc                                                                                             >  2:1656827/1‑150 (MQ=255)

                                                            ggCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTg                                                                                      >  1:279378/1‑150 (MQ=255)

                                                            ggCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTg                                                                                      <  2:3717842/150‑1 (MQ=255)

                                                            ggCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTg                                                                                      <  1:609147/150‑1 (MQ=255)

                                                             gCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGa                                                                                     >  1:3735676/1‑150 (MQ=255)

                                                              cTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAg                                                                                    >  2:3129821/1‑150 (MQ=255)

                                                               ttCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGc                                                                                   >  2:1233750/1‑150 (MQ=255)

                                                               ttCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGc                                                                                   <  1:2634630/150‑1 (MQ=255)

                                                                 catcatCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCa                                                                                 <  2:1555703/150‑1 (MQ=255)

                                                                   tcatcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGt                                                                               >  1:1835870/1‑150 (MQ=255)

                                                                     atcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGttt                                                                             >  2:805290/1‑150 (MQ=255)

                                                                     atcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGttt                                                                             >  1:52362/1‑150 (MQ=255)

                                                                      tcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGtttt                                                                            >  1:808456/1‑150 (MQ=255)

                                                                      tcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGtttt                                                                            >  1:2420294/1‑150 (MQ=255)

                                                                           cttcttGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTc                                                                       >  2:217858/1‑150 (MQ=255)

                                                                           cttcttGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTc                                                                       <  1:98778/150‑1 (MQ=255)

                                                                           cttcttGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTc                                                                       >  1:1047085/1‑150 (MQ=255)

                                                                                 gATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAg                                                                 >  2:1277984/1‑150 (MQ=255)

                                                                                 gATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAg                                                                 >  1:1757392/1‑150 (MQ=255)

                                                                                       gCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGgcgc                                                           <  1:337232/150‑1 (MQ=255)

                                                                                        cccAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGcgcg                                                          >  1:3692048/1‑150 (MQ=255)

                                                                                          cAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTc                                                        <  1:1014134/150‑1 (MQ=255)

                                                                                          cAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTc                                                        <  1:1617993/150‑1 (MQ=255)

                                                                                          cAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTc                                                        <  2:2261015/150‑1 (MQ=255)

                                                                                            ggTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCag                                                      >  1:2377905/1‑150 (MQ=255)

                                                                                             gTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCATTCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCaga                                                     <  1:178794/150‑1 (MQ=255)

                                                                                               aGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAgg                                                   <  2:2293305/150‑1 (MQ=255)

                                                                                                 aTGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCa                                                 >  1:713429/1‑150 (MQ=255)

                                                                                                    tCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCa                                              <  2:1969201/150‑1 (MQ=255)

                                                                                                    tCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCa                                              <  1:3028220/150‑1 (MQ=255)

                                                                                                      gCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCaaa                                            <  2:1216199/150‑1 (MQ=255)

                                                                                                      gCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCaaa                                            <  2:1721252/150‑1 (MQ=255)

                                                                                                         gAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGACCTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATc                                         >  1:191625/1‑150 (MQ=255)

                                                                                                          aGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCa                                        >  2:1565093/1‑150 (MQ=255)

                                                                                                               tttACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGt                                   <  2:751231/150‑1 (MQ=255)

                                                                                                               tttACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGt                                   >  1:2514154/1‑150 (MQ=255)

                                                                                                               tttACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGt                                   <  2:553297/150‑1 (MQ=255)

                                                                                                                 tACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTAt                                 <  2:152461/150‑1 (MQ=255)

                                                                                                                 tACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTAt                                 >  1:1642775/1‑150 (MQ=255)

                                                                                                                   ccATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTa                               <  1:2728207/150‑1 (MQ=255)

                                                                                                                   ccATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTa                               <  2:1789234/150‑1 (MQ=255)

                                                                                                                   ccATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTa                               <  2:3589294/150‑1 (MQ=255)

                                                                                                                          gCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATaa                        >  1:1572196/1‑150 (MQ=255)

                                                                                                                              cAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTg                    <  2:2576624/150‑1 (MQ=255)

                                                                                                                                 ttCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGttt                 <  2:3294765/150‑1 (MQ=255)

                                                                                                                                 ttCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGttt                 <  1:221340/150‑1 (MQ=255)

                                                                                                                                  tCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGtttt                <  1:2144558/150‑1 (MQ=255)

                                                                                                                                  tCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGtttt                <  2:2674223/150‑1 (MQ=255)

                                                                                                                                   cGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTa               >  1:2897042/1‑150 (MQ=255)

                                                                                                                                    gCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTAc              <  2:3203525/150‑1 (MQ=255)

                                                                                                                                      cAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGc            >  1:1708373/1‑150 (MQ=255)

                                                                                                                                        gACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCa          >  2:2825318/1‑150 (MQ=255)

                                                                                                                                         aCTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCAc         <  2:2044702/150‑1 (MQ=255)

                                                                                                                                         aCTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCAc         >  1:2323867/1‑150 (MQ=255)

                                                                                                                                          cTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCAcc        <  1:3782281/150‑1 (MQ=255)

                                                                                                                                          cTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCAcc        <  1:1446544/150‑1 (MQ=255)

                                                                                                                                            tCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCACCgg      >  2:1611145/1‑150 (MQ=255)

                                                                                                                                               gCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCACCGgaag   <  2:2372795/150‑1 (MQ=255)

                                                                                                                                                cccAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCACCGgaaga  <  2:3735676/150‑1 (MQ=255)

GCGGCGTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCTGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCACCGGAAGA  >  CP009273/3904406‑3904699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`