Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,931,021 C→A A123E (GCG→GAG)  rbsK → ribokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,931,0210CA99.2% 822.2 / ‑2.7 245A123E (GCG→GAG) rbsKribokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (132/111);  minor base G (1/1);  total (133/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

GCCAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCG  >  CP009273/3930874‑3931167
                                                                                                                                                   |                                                                                                                                                  
gCCAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGgagc                                                                                                                                                  <  1:2465665/150‑1 (MQ=255)
gCCAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGgagc                                                                                                                                                  >  2:718652/1‑150 (MQ=255)
gCCAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGgagc                                                                                                                                                  >  2:3144815/1‑150 (MQ=255)
 ccAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCt                                                                                                                                                 <  2:953093/150‑1 (MQ=255)
 ccAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCt                                                                                                                                                 <  1:753275/150‑1 (MQ=255)
  cagcagCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCTTGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCtg                                                                                                                                                <  1:2221696/150‑1 (MQ=255)
   agcagcTCGACACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGTATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCAGGAGCtgg                                                                                                                                               <  1:231270/150‑1 (MQ=255)
    gcagcTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCtggt                                                                                                                                              <  1:2778889/150‑1 (MQ=255)
     cagcTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCtggtg                                                                                                                                             <  2:395056/150‑1 (MQ=255)
      agcTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCtggtgg                                                                                                                                            <  2:222928/150‑1 (MQ=255)
            ccACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAgcgc                                                                                                                                      >  2:1046065/1‑150 (MQ=255)
            ccACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAgcgc                                                                                                                                      >  1:3772438/1‑150 (MQ=255)
            ccACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAgcgc                                                                                                                                      >  2:914324/1‑150 (MQ=255)
            ccACTGATAACATTGATAATACACCGGTCAGCGAGATCAAAGGCGAATCAACAGGAGAGGCGATGAATTTAGTTAATGGCGAAGGAGAGAAAGTCATAGGTATACAAGCCGGAGCTAATTCTGCACATACCCCGAAGCTGGAGCAAgcgc                                                                                                                                      >  2:2219998/1‑150 (MQ=255)
             cACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCa                                                                                                                                     >  2:3230300/1‑150 (MQ=255)
             cACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCa                                                                                                                                     <  2:301577/150‑1 (MQ=255)
             cACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCa                                                                                                                                     <  2:301567/150‑1 (MQ=255)
                 gATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGt                                                                                                                                 >  2:1991552/1‑150 (MQ=255)
                 gATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGt                                                                                                                                 >  2:3286240/1‑150 (MQ=255)
                 gATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGt                                                                                                                                 >  2:2677001/1‑150 (MQ=255)
                   tAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGa                                                                                                                               >  2:1356410/1‑150 (MQ=255)
                         tGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTAt                                                                                                                         >  1:2855584/1‑150 (MQ=255)
                          gATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTAtt                                                                                                                        <  2:1441893/150‑1 (MQ=255)
                           atatTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTg                                                                                                                       >  2:2807967/1‑150 (MQ=255)
                           atatTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTg                                                                                                                       >  1:3217384/1‑150 (MQ=255)
                              ttACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCa                                                                                                                    <  1:1634956/150‑1 (MQ=255)
                               tACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCaa                                                                                                                   <  1:1531881/150‑1 (MQ=255)
                                aCTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAAc                                                                                                                  <  1:2565650/150‑1 (MQ=255)
                                     ggTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTc                                                                                                             >  1:3410112/1‑150 (MQ=255)
                                       tCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAg                                                                                                           >  1:2885813/1‑150 (MQ=255)
                                        cAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGc                                                                                                          <  1:2522626/150‑1 (MQ=255)
                                         aGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCa                                                                                                         >  1:1594845/1‑150 (MQ=255)
                                            gTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCatta                                                                                                      >  1:1163259/1‑150 (MQ=255)
                                             tGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCattat                                                                                                     <  1:3415861/150‑1 (MQ=255)
                                              gATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAATGCGTCAGCattatt                                                                                                    <  1:3290981/150‑1 (MQ=255)
                                               aTCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCAttatta                                                                                                   <  2:2219665/150‑1 (MQ=255)
                                                  aaaGGCGAATCAACAGGTGTGGCGCTGATTTGTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATg                                                                                                <  2:3636790/150‑1 (MQ=255)
                                                    aGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGTAGGTGAGAATGTCATCGGTATTCATGTCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGTGCGTATTGCCAACGCGTCAGCATTATTAATGCa                                                                                              <  1:2441450/150‑1 (MQ=255)
                                                    aGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCa                                                                                              <  1:360192/150‑1 (MQ=255)
                                                        gAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTg                                                                                          >  2:1068373/1‑150 (MQ=255)
                                                         aaTCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTgg                                                                                         <  1:1545516/150‑1 (MQ=255)
                                                         aaTCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTgg                                                                                         <  2:3708425/150‑1 (MQ=255)
                                                            cAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAAt                                                                                      >  1:1761689/1‑150 (MQ=255)
                                                            cAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAAt                                                                                      >  1:1761484/1‑150 (MQ=255)
                                                             aaCAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATc                                                                                     <  2:2700104/150‑1 (MQ=255)
                                                             aaCAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATc                                                                                     >  1:1908084/1‑150 (MQ=255)
                                                              aCAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATca                                                                                    <  1:2290235/150‑1 (MQ=255)
                                                                aGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATcacc                                                                                  <  1:3230300/150‑1 (MQ=255)
                                                                   tgtgGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACt                                                                               <  1:1991552/150‑1 (MQ=255)
                                                                   tgtgGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACt                                                                               <  1:2677001/150‑1 (MQ=255)
                                                                         gcTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAg                                                                         >  2:2453678/1‑150 (MQ=255)
                                                                          cTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAgt                                                                        <  1:1028994/150‑1 (MQ=255)
                                                                           tGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAgtg                                                                       <  2:1823942/150‑1 (MQ=255)
                                                                             aTTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGtgtg                                                                     >  1:3438129/1‑150 (MQ=255)
                                                                                tttGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATg                                                                  >  2:1571430/1‑150 (MQ=255)
                                                                                   gTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCa                                                               <  1:384591/150‑1 (MQ=255)
                                                                                     tAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAgc                                                             >  2:3749150/1‑150 (MQ=255)
                                                                                      aaTGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAgcg                                                            >  2:1199678/1‑150 (MQ=255)
                                                                                        tGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAgcggc                                                          >  2:2796043/1‑150 (MQ=255)
                                                                                            gAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGaaa                                                      >  2:166918/1‑150 (MQ=255)
                                                                                            gAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGaaa                                                      >  1:2401969/1‑150 (MQ=255)
                                                                                            gAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGaaa                                                      >  2:73279/1‑150 (MQ=255)
                                                                                              cggTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCGTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAAt                                                    <  2:2884667/149‑1 (MQ=255)
                                                                                                  gagaATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGcc                                                >  2:1684413/1‑150 (MQ=255)
                                                                                                  gagaATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGcc                                                >  1:1366012/1‑150 (MQ=255)
                                                                                                   agaATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGccc                                               >  2:213879/1‑150 (MQ=255)
                                                                                                   agaATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGcca                                               <  1:1677523/150‑2 (MQ=255)
                                                                                                    gaATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCa                                              >  1:689249/1‑150 (MQ=255)
                                                                                                          cATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCAGTAGCTGGTGGAAGCGCAACGTGAGCTTATTGCCAACGAGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCATCGGCGAAAATCGCCCATCaaaa                                        >  2:618402/1‑150 (MQ=255)
                                                                                                           aTCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAAt                                       >  2:1433121/1‑150 (MQ=255)
                                                                                                           aTCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAAt                                       >  1:2114630/1‑150 (MQ=255)
                                                                                                            tCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATa                                      >  1:3486121/1‑150 (MQ=255)
                                                                                                                tATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGAc                                  <  1:2220098/150‑1 (MQ=255)
                                                                                                                 aTTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACt                                 <  2:3566039/150‑1 (MQ=255)
                                                                                                                 aTTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACt                                 <  1:3647600/150‑1 (MQ=255)
                                                                                                                 aTTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACt                                 >  1:2163223/1‑150 (MQ=255)
                                                                                                                   tCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTAt                               >  1:871183/1‑150 (MQ=255)
                                                                                                                    cATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATc                              <  2:2445051/150‑1 (MQ=255)
                                                                                                                     aTGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCg                             >  1:3111919/1‑150 (MQ=255)
                                                                                                                        ccGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTg                          >  2:3424975/1‑150 (MQ=255)
                                                                                                                        ccGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTg                          >  2:1019484/1‑150 (MQ=255)
                                                                                                                          ggCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTgcg                        <  1:2453678/150‑1 (MQ=255)
                                                                                                                          ggCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTgcg                        <  1:2814984/150‑1 (MQ=255)
                                                                                                                            cgcTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCt                      >  1:1109602/1‑150 (MQ=255)
                                                                                                                            cgcTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCt                      >  2:2124114/1‑150 (MQ=255)
                                                                                                                              cTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTa                    >  2:2345616/1‑150 (MQ=255)
                                                                                                                              cTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTa                    <  1:674646/150‑1 (MQ=255)
                                                                                                                               tAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTaa                   <  2:3486121/150‑1 (MQ=255)
                                                                                                                                 aTGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAAcc                 <  2:3323106/150‑1 (MQ=255)
                                                                                                                                 aTGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAAcc                 >  1:1138223/1‑150 (MQ=255)
                                                                                                                                  tgctgcCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAAccc                >  2:1562711/1‑150 (MQ=255)
                                                                                                                                    ctgcCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccgg              <  1:272450/150‑1 (MQ=255)
                                                                                                                                     tgcCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccggc             >  2:1323931/1‑150 (MQ=255)
                                                                                                                                     tgcCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccggc             >  1:3125867/1‑150 (MQ=255)
                                                                                                                                         ctgtCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccggctccg         <  2:2300860/147‑1 (MQ=255)
                                                                                                                                             ccccGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCcggctccggctc     <  2:3410112/150‑1 (MQ=255)
                                                                                                                                              cccGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTcg    >  1:3303296/1‑150 (MQ=255)
                                                                                                                                              cccGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTcg    <  2:2838310/150‑1 (MQ=255)
                                                                                                                                                cGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTcgcg  >  2:3601161/1‑150 (MQ=255)
                                                                                                                                                cGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTcgcg  <  2:2246205/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCG  >  CP009273/3930874‑3931167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑