Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,935,100	G→T	intergenic (‑413/‑68)	yieP ← / → rrsC	putative transcriptional regulator/16S ribosomal RNA of rrnC operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,935,100	0	G	T	100.0%	191.9 / NA	80	intergenic (‑413/‑68)	yieP/rrsC	putative transcriptional regulator/16S ribosomal RNA of rrnC operon
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (48/32);  total (48/32)

AAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAAC  >  CP009273/3934953‑3935163                                                                                                                                                    |                                                                aaatGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTaa                                                               >  1:3311044/1‑150 (MQ=21)  aatGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAAt                                                              >  1:2459592/1‑150 (MQ=21)   atGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATa                                                             <  1:2265137/150‑1 (MQ=21)   atGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATa                                                             >  1:2764084/1‑150 (MQ=21)    tGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATAc                                                            >  1:255016/1‑150 (MQ=21)    tGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATAc                                                            >  1:254824/1‑150 (MQ=21)    tGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATAc                                                            >  2:1626573/1‑150 (MQ=21)     gCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATAcc                                                           >  1:2699246/1‑150 (MQ=21)      cTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGATAAATTAATACCa                                                          >  1:1654997/1‑150 (MQ=21)      cTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCa                                                          >  2:15180/1‑150 (MQ=21)      cTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCa                                                          >  1:2072366/1‑150 (MQ=21)      cTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCa                                                          >  1:2072064/1‑150 (MQ=21)      cTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCa                                                          <  1:2731891/150‑1 (MQ=21)      cTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCa                                                          >  1:1023143/1‑150 (MQ=21)       ttGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCaa                                                         >  1:125780/1‑150 (MQ=21)        tGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAg                                                        >  2:815494/1‑150 (MQ=21)          aCTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGtc                                                      >  1:3644446/1‑150 (MQ=21)          aCTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGtc                                                      <  2:1797882/150‑1 (MQ=21)           ctctGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGtct                                                     <  1:1625203/150‑1 (MQ=21)            tctGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGtctc                                                    >  2:3664234/1‑150 (MQ=21)              tGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCaa                                                  <  1:3016363/150‑1 (MQ=21)              tGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCaa                                                  >  2:2316391/1‑150 (MQ=21)                 aGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAagag                                               <  2:1271714/150‑1 (MQ=21)                 aGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAagag                                               >  2:1179315/1‑150 (MQ=21)                 aGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAagag                                               >  2:740729/1‑150 (MQ=21)                 aGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAagag                                               >  2:517600/1‑150 (MQ=21)                 aGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAagag                                               <  2:163577/150‑1 (MQ=21)                 aGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAagag                                               >  2:1725703/1‑150 (MQ=21)                  gCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGt                                              <  2:1539969/150‑1 (MQ=21)                  gCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGt                                              <  2:3210366/150‑1 (MQ=21)                    gggAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGa                                            >  1:1563519/1‑150 (MQ=21)                    gggAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGa                                            >  1:1563507/1‑150 (MQ=21)                    gggAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGa                                            >  1:1563504/1‑150 (MQ=21)                     ggAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGaa                                           <  1:1135189/150‑1 (MQ=21)                      gAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGGACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAac                                          <  2:1989458/150‑1 (MQ=21)                       aaaGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAaca                                         >  2:923441/1‑150 (MQ=21)                       aaaGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAaca                                         >  1:2575978/1‑150 (MQ=21)                       aaaGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAaca                                         <  1:2806607/150‑1 (MQ=21)                         aGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACg                                       <  2:47929/150‑1 (MQ=17)                            gTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTaa                                    >  2:2227790/1‑150 (MQ=17)                            gTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTaa                                    >  2:2227830/1‑150 (MQ=17)                              attatGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAAtt                                  <  1:2449405/150‑1 (MQ=17)                              attatGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAAtt                                  <  2:2567169/150‑1 (MQ=17)                                tatGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCa                                >  1:2644665/1‑150 (MQ=17)                                tatGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCa                                >  1:738484/1‑150 (MQ=17)                                 atGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCAt                               >  1:3650397/1‑150 (MQ=17)                                    cacaCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTAc                            >  2:3336174/1‑150 (MQ=17)                                     acacCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACg                           <  2:661084/150‑1 (MQ=17)                                     acacCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACg                           <  1:1759072/150‑1 (MQ=17)                                     acacCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACg                           >  1:1326983/1‑150 (MQ=17)                                     acacCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTGATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACg                           <  2:1207462/150‑1 (MQ=17)                                        ccccGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAg                        <  1:1358178/150‑1 (MQ=17)                                         cccGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGt                       <  1:866062/150‑1 (MQ=17)                                          ccGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGtt                      <  2:26444/150‑1 (MQ=17)                                           cgcgCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGTGTGAACACGTAATTCATTACGAAGttt                     >  2:1099607/1‑150 (MQ=17)                                           cgcgCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGttt                     <  2:982858/150‑1 (MQ=17)                                           cgcgCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGttt                     >  2:3282375/1‑150 (MQ=17)                                            gcgcCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTa                    >  1:925893/1‑150 (MQ=17)                                              gccgcTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAAt                  >  1:3519004/1‑150 (MQ=17)                                               ccgcTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAAtt                 <  2:1689367/150‑1 (MQ=17)                                               ccgcTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAAtt                 <  2:1689210/150‑1 (MQ=17)                                               ccgcTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAAtt                 <  2:2025015/150‑1 (MQ=17)                                                cgcTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGTGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTc                <  2:511341/150‑1 (MQ=17)                                                 gcTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCt               >  2:1967590/1‑150 (MQ=17)                                                 gcTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCt               >  2:55328/1‑150 (MQ=17)                                                  cTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCtt              >  2:3406781/1‑150 (MQ=17)                                                  cTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCtt              <  1:3249495/150‑1 (MQ=17)                                                    gagaAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTg            >  2:663340/1‑150 (MQ=17)                                                     agaAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGa           >  2:2941687/1‑150 (MQ=17)                                                     agaAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGa           <  1:3280699/150‑1 (MQ=17)                                                      gaAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAg          <  2:2569983/150‑1 (MQ=17)                                                       aaaaaGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGc         >  1:1528461/1‑150 (MQ=17)                                                        aaaaGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCa        >  1:3200482/1‑150 (MQ=17)                                                         aaagcaaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCAt       <  1:730881/150‑1 (MQ=17)                                                          aagcaaagcGGCACTGCTCGTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATc      >  2:2802036/1‑150 (MQ=17)                                                           agcatagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCa     <  1:3450308/150‑1 (MQ=14)                                                           agcaaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCa     >  1:2362511/1‑150 (MQ=17)                                                            gcaaagcGGCACTGCTCTTTAACAATTAATCAGACAATCTGTGTGGGCACACGAAGAAACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCACAAGAGTAAACACGTAATTCATTACTAAGTTTAATTCTTTGAGCATCaa    >  1:731725/1‑150 (MQ=11)                                                             caaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCaaa   >  1:1382996/1‑150 (MQ=14)                                                              aaagcGGCACTGCTCTATAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATTAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAAc  <  2:2336630/150‑1 (MQ=11)                                                                                                                                                    |                                                                AAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAAC  >  CP009273/3934953‑3935163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑