Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,972,296 G→T E112D (GAG→GAT)  wzyE → putative ECA polysaccharide chain elongation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,972,2960GT100.0% 821.8 / NA 255E112D (GAG→GAT) wzyEputative ECA polysaccharide chain elongation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (110/145);  total (110/145)

Only 100 of 255 total aligned reads displayed.

GGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGAGACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACG  >  CP009273/3972150‑3972439
                                                                                                                                                  |                                                                                                                                               
ggTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATAcc                                                                                                                                              <  2:626935/150‑1 (MQ=255)
ggTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATAcc                                                                                                                                              <  2:3381331/150‑1 (MQ=255)
  tgtCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCaa                                                                                                                                            >  1:714927/1‑150 (MQ=255)
     cgcgCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCt                                                                                                                                         <  2:3530302/150‑1 (MQ=255)
     cgcgCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCt                                                                                                                                         <  2:1635066/150‑1 (MQ=255)
      gcgcCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCtt                                                                                                                                        <  2:759167/150‑1 (MQ=255)
      gcgcCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCtt                                                                                                                                        >  1:1474702/1‑150 (MQ=255)
      gcgcCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCtt                                                                                                                                        <  2:738176/150‑1 (MQ=255)
      gcgcCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCtt                                                                                                                                        <  1:509140/150‑1 (MQ=255)
       cgccgcCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTa                                                                                                                                       >  1:495317/1‑150 (MQ=255)
       cgccgcCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTa                                                                                                                                       <  2:1296628/150‑1 (MQ=255)
       cgccgcCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTa                                                                                                                                       >  2:2010087/1‑150 (MQ=255)
       cgccgcCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTa                                                                                                                                       >  2:3141866/1‑150 (MQ=255)
        gccgccAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTAc                                                                                                                                      <  1:839488/150‑1 (MQ=255)
          cgccAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGt                                                                                                                                    >  2:3057788/1‑150 (MQ=255)
           gccAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTg                                                                                                                                   <  2:2635691/150‑1 (MQ=255)
             cAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTggg                                                                                                                                 >  2:1416503/1‑150 (MQ=255)
             cAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTggg                                                                                                                                 >  1:2651221/1‑150 (MQ=255)
              aGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGt                                                                                                                                >  1:3379060/1‑150 (MQ=255)
                aaaTCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGa                                                                                                                              >  1:3777014/1‑150 (MQ=255)
                 aaTCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGAt                                                                                                                             >  1:2096435/1‑150 (MQ=255)
                    cTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCt                                                                                                                          <  1:3700013/150‑1 (MQ=255)
                    cTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCt                                                                                                                          <  2:1884396/150‑1 (MQ=255)
                    cTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCt                                                                                                                          <  2:3602873/150‑1 (MQ=255)
                      tgttgCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGa                                                                                                                        >  1:653640/1‑150 (MQ=255)
                        ttgCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATg                                                                                                                      >  2:68972/1‑150 (MQ=255)
                         tgCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATgg                                                                                                                     >  1:3605057/1‑150 (MQ=255)
                          gCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATggg                                                                                                                    >  1:1505468/1‑150 (MQ=255)
                           cAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGt                                                                                                                   >  2:658964/1‑150 (MQ=255)
                           cAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGt                                                                                                                   >  2:2723034/1‑150 (MQ=255)
                           cAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGt                                                                                                                   <  2:827805/150‑1 (MQ=255)
                            aGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTa                                                                                                                  >  1:2889243/1‑150 (MQ=255)
                             ggCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTAt                                                                                                                 <  2:1122854/150‑1 (MQ=255)
                               cGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATcg                                                                                                               <  2:2992476/150‑1 (MQ=255)
                                gTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATcgc                                                                                                              >  2:2948000/1‑150 (MQ=255)
                                 ttGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATcgcg                                                                                                             >  2:2281618/1‑150 (MQ=255)
                                    cTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTg                                                                                                          <  1:2129092/150‑1 (MQ=255)
                                      ttCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGt                                                                                                        <  1:1769236/150‑1 (MQ=255)
                                      ttCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGt                                                                                                        <  1:2186541/150‑1 (MQ=255)
                                           cGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCg                                                                                                   >  1:3420249/1‑150 (MQ=255)
                                           cGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCg                                                                                                   <  1:1359022/150‑1 (MQ=255)
                                             ggCTGCTTCTACGCGGTTTACTATGTCACCTACAACACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTc                                                                                                 <  2:1961699/150‑1 (MQ=255)
                                             ggCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTc                                                                                                 <  1:924774/150‑1 (MQ=255)
                                             ggCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTc                                                                                                 <  1:2829095/150‑1 (MQ=255)
                                                tgctTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGc                                                                                              <  2:2402602/150‑1 (MQ=255)
                                                  ctTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCAt                                                                                            <  2:3790494/150‑1 (MQ=255)
                                                       aCGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCAtcttct                                                                                       >  2:86205/1‑150 (MQ=255)
                                                         gcgGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCttcttc                                                                                     <  2:638561/150‑1 (MQ=255)
                                                          cgGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCa                                                                                    <  2:2029634/150‑1 (MQ=255)
                                                             tttACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGc                                                                                 >  2:1755443/1‑150 (MQ=255)
                                                               tACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGcac                                                                               >  1:3444405/1‑150 (MQ=255)
                                                               tACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGcac                                                                               >  1:2459635/1‑150 (MQ=255)
                                                                     gTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGc                                                                         >  1:3156772/1‑150 (MQ=255)
                                                                     gTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGc                                                                         >  1:2514462/1‑150 (MQ=255)
                                                                      tCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCt                                                                        <  1:3454592/150‑1 (MQ=255)
                                                                         ccTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCtttt                                                                     <  2:448389/150‑1 (MQ=255)
                                                                          cTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCttttt                                                                    >  1:3542542/1‑150 (MQ=255)
                                                                             cAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCt                                                                 <  1:2941104/150‑1 (MQ=255)
                                                                               aaaCCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGt                                                               <  1:2974836/150‑1 (MQ=255)
                                                                                   ccGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCg                                                           <  2:578100/150‑1 (MQ=255)
                                                                                   ccGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCg                                                           <  2:2091267/150‑1 (MQ=255)
                                                                                     gCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGc                                                         >  1:1322838/1‑150 (MQ=255)
                                                                                       cTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCtt                                                       >  1:1003285/1‑150 (MQ=255)
                                                                                       cTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCtt                                                       >  1:207776/1‑150 (MQ=255)
                                                                                       cTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCtt                                                       <  1:1291660/150‑1 (MQ=255)
                                                                                          cGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAAc                                                    <  2:3237778/150‑1 (MQ=255)
                                                                                            cAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTc                                                  >  2:436129/1‑150 (MQ=255)
                                                                                             aaaCGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTcc                                                 <  1:2619275/150‑1 (MQ=255)
                                                                                             aaaCGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATCGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTcc                                                 >  2:2476932/1‑150 (MQ=255)
                                                                                                cgcgTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTAc                                              <  1:1020621/150‑1 (MQ=255)
                                                                                                 gcgTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACa                                             <  2:1474702/150‑1 (MQ=255)
                                                                                                   gTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGt                                           >  1:1934125/1‑150 (MQ=255)
                                                                                                    ttGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTc                                          <  2:2501092/150‑1 (MQ=255)
                                                                                                     tGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCa                                         <  1:2399158/150‑1 (MQ=255)
                                                                                                      gCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAg                                        >  1:2904567/1‑150 (MQ=255)
                                                                                                       cTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGa                                       >  2:3585635/1‑150 (MQ=255)
                                                                                                         gATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATc                                     <  2:1375167/150‑1 (MQ=255)
                                                                                                         gATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGCATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATc                                     >  2:347353/1‑150 (MQ=255)
                                                                                                               ccGCGCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTcc                               <  1:3353221/150‑1 (MQ=255)
                                                                                                                cgcgCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCa                              <  1:1169624/150‑1 (MQ=255)
                                                                                                                cgcgCCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCa                              >  2:3709442/1‑150 (MQ=255)
                                                                                                                  cgcCGTCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGt                            <  1:2074996/150‑1 (MQ=255)
                                                                                                                       tCCGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGt                       >  1:2704832/1‑150 (MQ=255)
                                                                                                                         cGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGtct                     <  1:575657/150‑1 (MQ=255)
                                                                                                                         cGCTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGtct                     >  2:1536105/1‑150 (MQ=255)
                                                                                                                           cTGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTcc                   <  1:107906/150‑1 (MQ=255)
                                                                                                                            tGTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCg                  <  2:2283311/150‑1 (MQ=255)
                                                                                                                             gTTTACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCgg                 >  1:730693/1‑150 (MQ=255)
                                                                                                                              tttACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCggc                >  2:1714888/1‑150 (MQ=255)
                                                                                                                               ttACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCggcg               >  1:334354/1‑150 (MQ=255)
                                                                                                                                tACCATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCggcgt              >  2:2864856/1‑150 (MQ=255)
                                                                                                                                   cATGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCggcgtggc           <  1:3515056/150‑1 (MQ=255)
                                                                                                                                     tGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTTTCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCggcgtggcgt         <  2:3508149/150‑1 (MQ=255)
                                                                                                                                     tGAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCggcgtggcgt         <  2:1611976/150‑1 (MQ=255)
                                                                                                                                      gAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGtt        >  1:1122152/1‑150 (MQ=255)
                                                                                                                                      gAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGtt        >  2:2621145/1‑150 (MQ=255)
                                                                                                                                      gAACCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGtt        >  1:3014780/1‑150 (MQ=255)
                                                                                                                                       aaCCGCGTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTa       <  2:1344231/150‑1 (MQ=255)
                                                                                                                                            cgTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACg  <  1:2319467/150‑1 (MQ=255)
                                                                                                                                            cgTGGATACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACg  <  2:936059/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGCTTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGAGACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACG  >  CP009273/3972150‑3972439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑