Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,983,298 G→T R277S (CGC→AGC)  hemC ← hydroxymethylbilane synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,983,2980GT99.5% 677.5 / ‑6.3 202R277S (CGC→AGC) hemChydroxymethylbilane synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (99/102);  minor base C (1/0);  total (100/102)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 205 total aligned reads displayed.

CTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATGGCGCGTTCTGCGGTAA  >  CP009273/3983152‑3983444
                                                                                                                                                  |                                                                                                                                                  
ctcCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCgctgcg                                                                                                                                                 >  1:143139/1‑150 (MQ=255)
   cAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTc                                                                                                                                              >  1:1028468/1‑150 (MQ=255)
   cAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTc                                                                                                                                              >  1:941645/1‑150 (MQ=255)
   cAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTc                                                                                                                                              >  1:484914/1‑150 (MQ=255)
   cAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTc                                                                                                                                              >  1:483721/1‑150 (MQ=255)
   cAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTc                                                                                                                                              >  1:2931443/1‑150 (MQ=255)
   cAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTc                                                                                                                                              <  2:2856189/150‑1 (MQ=255)
   cAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCCGCGTTc                                                                                                                                              >  2:1557217/1‑150 (MQ=255)
      cGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCAcc                                                                                                                                           <  2:3710596/150‑1 (MQ=255)
       gggAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACcg                                                                                                                                          >  2:2597821/1‑150 (MQ=255)
        ggAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACcgc                                                                                                                                         >  1:3175552/1‑150 (MQ=255)
        ggAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACcgc                                                                                                                                         >  1:62020/1‑150 (MQ=255)
         gagaCGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACcgcg                                                                                                                                        >  1:2081535/1‑150 (MQ=255)
          agaCGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGa                                                                                                                                       >  1:2983597/1‑150 (MQ=255)
            aCGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGaat                                                                                                                                     >  2:3695602/1‑150 (MQ=255)
            aCGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGaat                                                                                                                                     >  2:1128084/1‑150 (MQ=255)
              gggcgggTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGaataa                                                                                                                                   >  2:1494960/1‑150 (MQ=255)
                  gggTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTg                                                                                                                               >  2:3726367/1‑150 (MQ=255)
                  gggTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTg                                                                                                                               >  2:1833550/1‑150 (MQ=255)
                       aCAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAAc                                                                                                                          <  2:3648381/150‑1 (MQ=255)
                            gATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTcc                                                                                                                     >  2:1705525/1‑150 (MQ=255)
                                cTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGgcg                                                                                                                 >  2:2137235/1‑150 (MQ=255)
                                 tCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGgcgc                                                                                                                >  1:2388734/1‑150 (MQ=255)
                                  cATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGgcgcg                                                                                                               <  1:476123/150‑1 (MQ=255)
                                              tctcCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgca                                                                                                   <  2:3355884/150‑1 (MQ=255)
                                               ctcCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcac                                                                                                  <  2:2925726/150‑1 (MQ=255)
                                                 ccGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgc                                                                                                <  2:3417287/150‑1 (MQ=255)
                                                   gTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAg                                                                                              <  2:624774/150‑1 (MQ=255)
                                                   gTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAg                                                                                              >  2:1618573/1‑150 (MQ=255)
                                                    ttATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGc                                                                                             <  2:2070933/150‑1 (MQ=255)
                                                     tataGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGcc                                                                                            <  1:215598/150‑1 (MQ=255)
                                                     tataGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGcc                                                                                            >  2:3120345/1‑150 (MQ=255)
                                                     tataGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGcc                                                                                            <  2:3654609/150‑1 (MQ=255)
                                                      ataGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCa                                                                                           >  1:2297985/1‑150 (MQ=255)
                                                      ataGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCa                                                                                           >  1:1351646/1‑150 (MQ=255)
                                                      ataGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCa                                                                                           >  1:1351688/1‑150 (MQ=255)
                                                          aCTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAtt                                                                                       <  2:1990819/150‑1 (MQ=255)
                                                            ttCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTc                                                                                     >  2:2229070/1‑150 (MQ=255)
                                                            ttCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTc                                                                                     >  2:321486/1‑150 (MQ=255)
                                                               aGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGcc                                                                                  >  2:2245659/1‑150 (MQ=255)
                                                               aGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGcc                                                                                  >  1:1242116/1‑150 (MQ=255)
                                                                gCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCa                                                                                 >  1:1872265/1‑150 (MQ=255)
                                                                 cGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCAt                                                                                >  1:3629806/1‑150 (MQ=255)
                                                                  gAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATc                                                                               >  2:1239776/1‑150 (MQ=255)
                                                                  gAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATc                                                                               >  2:462970/1‑150 (MQ=255)
                                                                  gAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATc                                                                               >  2:738091/1‑150 (MQ=255)
                                                                  gAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATc                                                                               <  1:1117303/150‑1 (MQ=255)
                                                                  gAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATc                                                                               <  1:1117396/150‑1 (MQ=255)
                                                                     gATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAt                                                                            <  1:688642/150‑1 (MQ=255)
                                                                       tctcGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATaa                                                                          >  1:2771809/1‑150 (MQ=255)
                                                                        ctcGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAg                                                                         >  2:2116274/1‑150 (MQ=255)
                                                                         tcGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGc                                                                        >  2:565110/1‑150 (MQ=255)
                                                                              cgcgcCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGc                                                                   <  1:797704/150‑1 (MQ=255)
                                                                                 gcCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTa                                                                <  2:257690/150‑1 (MQ=255)
                                                                                 gcCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTa                                                                >  1:2188825/1‑150 (MQ=255)
                                                                                 gcCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTa                                                                <  1:1782591/150‑1 (MQ=255)
                                                                                    gTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCt                                                             >  1:1540543/1‑150 (MQ=255)
                                                                                      tATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAc                                                           <  2:1662431/150‑1 (MQ=255)
                                                                                      tATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAc                                                           <  2:3556703/150‑1 (MQ=255)
                                                                                        ttCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCa                                                         <  2:2055561/150‑1 (MQ=255)
                                                                                           aGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAtt                                                      >  1:2506836/1‑150 (MQ=255)
                                                                                            gTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTg                                                     <  1:1128084/150‑1 (MQ=255)
                                                                                              aGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGc                                                   >  2:1069157/1‑150 (MQ=255)
                                                                                              aGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGc                                                   <  2:3290792/150‑1 (MQ=255)
                                                                                                  cttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCt                                               >  2:151642/1‑150 (MQ=255)
                                                                                                  cttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCt                                               >  2:1814150/1‑150 (MQ=255)
                                                                                                  cttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCt                                               <  1:1405270/150‑1 (MQ=255)
                                                                                                   ttctGCCAGCGAAATCCCGATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTg                                              >  2:2662033/1‑150 (MQ=255)
                                                                                                   ttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTg                                              <  2:2492085/150‑1 (MQ=255)
                                                                                                      tGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACa                                           <  2:143139/150‑1 (MQ=255)
                                                                                                      tGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACa                                           >  1:3516367/1‑150 (MQ=255)
                                                                                                       gCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACAt                                          <  2:1721078/150‑1 (MQ=255)
                                                                                                       gCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACAt                                          >  1:3247628/1‑150 (MQ=255)
                                                                                                         cAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATcc                                        >  1:1547520/1‑150 (MQ=255)
                                                                                                         cAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATcc                                        <  1:1494960/150‑1 (MQ=255)
                                                                                                         cAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATcc                                        <  1:881134/150‑1 (MQ=255)
                                                                                                          aNCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCg                                       >  1:1164456/1‑150 (MQ=255)
                                                                                                          aGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCg                                       <  2:262260/150‑1 (MQ=255)
                                                                                                          aGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCg                                       <  1:2657009/150‑1 (MQ=255)
                                                                                                          aGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCg                                       >  2:1521145/1‑150 (MQ=255)
                                                                                                             gAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCt                                    <  2:3072305/150‑1 (MQ=255)
                                                                                                              aaaTCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGATCCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCtt                                   <  1:1833550/150‑1 (MQ=255)
                                                                                                              aaaTCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCtt                                   <  2:1388652/150‑1 (MQ=255)
                                                                                                              aaaTCCCCATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCtt                                   <  1:3661844/150‑1 (MQ=255)
                                                                                                                    ccATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAc                             <  2:1214249/150‑1 (MQ=255)
                                                                                                                    ccATTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAc                             <  2:62020/150‑1 (MQ=255)
                                                                                                                      aTTTGTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTCCCAATTGGCACCTGACCTCCGCCTTCGAGACgg                           >  1:1601526/1‑150 (MQ=255)
                                                                                                                          gTTCGGCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTAt                       <  1:242261/150‑1 (MQ=255)
                                                                                                                              ggCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCAt                   >  1:1566285/1‑150 (MQ=255)
                                                                                                                               gCATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATg                  <  2:126794/150‑1 (MQ=255)
                                                                                                                                cATCTTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATgg                 <  1:3587068/150‑1 (MQ=255)
                                                                                                                                 aTCGTGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGTCCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATGgc                <  1:2614812/150‑1 (MQ=255)
                                                                                                                                     tGCGGCGCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATGGCGCGt            >  1:2444191/1‑150 (MQ=255)
                                                                                                                                       tggcgCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATGGCGCGTTc          >  1:3739903/2‑150 (MQ=255)
                                                                                                                                       cggcgCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATGGCGCGTTc          <  2:2338445/150‑1 (MQ=255)
                                                                                                                                       cggcgCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATGGCGCGTTc          <  2:1895575/150‑1 (MQ=255)
                                                                                                                                        ggcgCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATGGCGCGTTCt         >  2:379085/1‑150 (MQ=255)
                                                                                                                                        ggcgCACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATGGCGCGTTCt         >  1:2555798/1‑150 (MQ=255)
                                                                                                                                           gcACCGCTGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATGGCGCGTTCTGCg      >  1:1277371/1‑150 (MQ=255)
                                                                                                                                               cgctGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATGGCGCGTTCTGCGGTaa  >  1:3682465/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATGGCGCGTTCTGCGGTAA  >  CP009273/3983152‑3983444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑