Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,036,012 G→T D80Y (GAT→TAT)  srkA → stress response kinase A; MazF antagonist; Thr/Ser kinase involved in Cpx stress response

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,036,0120GT99.5% 717.7 / ‑5.2 218D80Y (GAT→TAT) srkAstress response kinase A; MazF antagonist; Thr/Ser kinase involved in Cpx stress response
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (116/101);  minor base C (1/0);  total (117/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 224 total aligned reads displayed.

CTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATGATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTAT  >  CP009273/4035865‑4036158
                                                                                                                                                   |                                                                                                                                                  
cTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAattat                                                                                                                                                  <  1:517399/150‑1 (MQ=255)
      ccGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGtt                                                                                                                                            >  1:3015278/1‑150 (MQ=255)
       cGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTc                                                                                                                                           >  2:1755070/1‑150 (MQ=255)
        gCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGCCGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTcc                                                                                                                                          >  2:171414/1‑150 (MQ=255)
        gCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTcc                                                                                                                                          <  2:673015/150‑1 (MQ=255)
         cTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCg                                                                                                                                         <  1:1606304/150‑1 (MQ=255)
          ttAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCgg                                                                                                                                        <  1:2673383/150‑1 (MQ=255)
           tAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGt                                                                                                                                       >  1:1416556/1‑150 (MQ=255)
            aaCAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTc                                                                                                                                      <  1:1914491/150‑1 (MQ=255)
             aCAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCg                                                                                                                                     <  1:3658720/150‑1 (MQ=255)
               aGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTCCATTATGAAGTTCCGGTCgca                                                                                                                                   >  2:3308507/1‑150 (MQ=255)
                  tATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCgcagca                                                                                                                                >  2:262325/1‑150 (MQ=255)
                   aTGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGAACAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCAc                                                                                                                               >  1:948106/1‑150 (MQ=255)
                     gAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCt                                                                                                                             >  2:870513/1‑150 (MQ=255)
                     gAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCt                                                                                                                             >  1:68787/1‑150 (MQ=255)
                        aaCCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCtgtg                                                                                                                          >  2:1444565/1‑150 (MQ=255)
                              gtCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCttt                                                                                                                    <  1:3604687/150‑1 (MQ=255)
                              gtCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCttt                                                                                                                    <  2:539202/150‑1 (MQ=255)
                               tCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTa                                                                                                                   >  2:3282265/1‑150 (MQ=255)
                                    cAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGt                                                                                                              >  1:1055999/1‑150 (MQ=255)
                                    cAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGt                                                                                                              >  1:132134/1‑150 (MQ=255)
                                    cAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGt                                                                                                              >  1:2064993/1‑150 (MQ=255)
                                     aaTTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCGCCTGTGGCCTTTAACGGTc                                                                                                             >  2:134103/1‑150 (MQ=255)
                                       tttCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAg                                                                                                           <  1:3532603/150‑1 (MQ=255)
                                       tttCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAg                                                                                                           >  1:3377616/1‑150 (MQ=255)
                                         tCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGAc                                                                                                         >  1:923087/1‑150 (MQ=255)
                                          cAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACt                                                                                                        <  2:252808/150‑1 (MQ=255)
                                            ggACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACttt                                                                                                      <  2:1285999/150‑1 (MQ=255)
                                            ggACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACttt                                                                                                      <  2:368324/150‑1 (MQ=255)
                                            ggACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACttt                                                                                                      <  1:334463/150‑1 (MQ=255)
                                             gACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTa                                                                                                     >  2:135407/1‑150 (MQ=255)
                                              aCGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTAt                                                                                                    >  1:2394285/1‑150 (MQ=255)
                                                gAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTg                                                                                                  >  1:181977/1‑150 (MQ=255)
                                                  aGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGaa                                                                                                >  2:3172518/1‑150 (MQ=255)
                                                  aGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGaa                                                                                                >  1:1214803/1‑150 (MQ=255)
                                                   gATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAat                                                                                               >  2:639237/1‑150 (MQ=255)
                                                   gATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAat                                                                                               <  1:2127472/150‑1 (MQ=255)
                                                       gtcgACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAatcatc                                                                                           >  2:243050/1‑150 (MQ=255)
                                                       gtcgACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAatcatc                                                                                           >  2:3094795/1‑150 (MQ=255)
                                                        tcgACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAAtcatca                                                                                          <  2:2645087/150‑1 (MQ=255)
                                                         cgACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAg                                                                                         >  2:713647/1‑150 (MQ=255)
                                                             gTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGAt                                                                                     >  1:138389/1‑150 (MQ=255)
                                                              ttttGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGAtt                                                                                    <  2:810249/150‑1 (MQ=255)
                                                               tttGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGAttt                                                                                   <  1:427272/150‑1 (MQ=255)
                                                               tttGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGAttt                                                                                   <  2:480755/150‑1 (MQ=255)
                                                                    cgtcAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCACTTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTAttt                                                                              <  2:2781997/150‑1 (MQ=255)
                                                                      tcAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCg                                                                            <  1:135407/150‑1 (MQ=255)
                                                                       cAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGc                                                                           >  2:885934/1‑150 (MQ=255)
                                                                       cAAATTTTATCGCCCAGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGc                                                                           >  1:2545105/1‑150 (MQ=255)
                                                                        aaaTTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCt                                                                          >  2:2397384/1‑150 (MQ=255)
                                                                          aTTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGt                                                                        <  1:2291314/150‑1 (MQ=255)
                                                                             ttATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGtttt                                                                     >  1:3716164/1‑150 (MQ=255)
                                                                              tATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGttttt                                                                    <  2:1227640/150‑1 (MQ=255)
                                                                              tATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGttttt                                                                    >  1:2161209/1‑150 (MQ=255)
                                                                               aTCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTc                                                                   >  2:1484914/1‑150 (MQ=255)
                                                                               aTCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTc                                                                   >  2:1735538/1‑150 (MQ=255)
                                                                                 cGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCa                                                                 >  1:2506345/1‑150 (MQ=255)
                                                                                  gCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCaa                                                                >  2:98204/1‑150 (MQ=255)
                                                                                  gCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCaa                                                                >  1:510174/1‑150 (MQ=255)
                                                                                     cTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCg                                                             >  2:1895197/1‑150 (MQ=255)
                                                                                       gAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTc                                                           <  1:2194108/150‑1 (MQ=255)
                                                                                       gAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTc                                                           <  1:2858974/150‑1 (MQ=255)
                                                                                        aaCGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCg                                                          <  2:1010290/150‑1 (MQ=255)
                                                                                        aaCGTTGGACAGCAGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCg                                                          >  1:3470974/1‑150 (MQ=255)
                                                                                         aCGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCgg                                                         <  2:1393622/150‑1 (MQ=255)
                                                                                              ggACAGCCGATCAAATCCTCGAAGAACATCAATTGGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTc                                                    <  1:2046829/150‑1 (MQ=255)
                                                                                                aCAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGc                                                  >  2:938823/1‑150 (MQ=255)
                                                                                                  aGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCa                                                <  2:1566230/150‑1 (MQ=255)
                                                                                                  aGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCa                                                >  1:1547809/1‑150 (MQ=255)
                                                                                                     cGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGtt                                             <  1:559330/150‑1 (MQ=255)
                                                                                                      gATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGATGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGTTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAATCGTCGGTGGTCGCCAGTTc                                            >  1:85011/1‑150 (MQ=255)
                                                                                                      gATCAAATCCTCGAAGAACATCAATGTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTc                                            <  2:2947223/150‑1 (MQ=255)
                                                                                                        tCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGa                                          >  2:267388/1‑150 (MQ=255)
                                                                                                        tCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGa                                          <  1:1753478/150‑1 (MQ=255)
                                                                                                           aaTCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGc                                       >  2:1526758/1‑150 (MQ=255)
                                                                                                           aaTCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGc                                       <  2:2238364/150‑1 (MQ=255)
                                                                                                            aTCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCt                                      >  1:3046836/1‑150 (MQ=255)
                                                                                                                tCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGata                                  >  1:1097920/1‑150 (MQ=255)
                                                                                                                tCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGata                                  >  1:3660326/1‑150 (MQ=255)
                                                                                                                  gaagaaCATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGataat                                >  1:2060129/1‑150 (MQ=255)
                                                                                                                   aagaaCATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGataata                               >  1:2598815/1‑150 (MQ=255)
                                                                                                                      aaCATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCg                            >  2:2427822/1‑150 (MQ=255)
                                                                                                                      aaCATCAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCg                            <  2:2874500/150‑1 (MQ=255)
                                                                                                                           cAATTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAg                       <  1:1958828/150‑1 (MQ=255)
                                                                                                                             aTTTGCGTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGAt                     >  2:751140/1‑150 (MQ=255)
                                                                                                                                   gTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGc               <  2:3711458/150‑1 (MQ=255)
                                                                                                                                   gTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGc               >  1:2474919/1‑150 (MQ=255)
                                                                                                                                   gTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGc               <  2:2064993/150‑1 (MQ=255)
                                                                                                                                   gTTGCAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGACAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGc               <  2:2424320/150‑1 (MQ=255)
                                                                                                                                       cAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGtt           >  2:444665/1‑150 (MQ=255)
                                                                                                                                       cAGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGtt           <  1:3111334/150‑1 (MQ=255)
                                                                                                                                        aGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTg          <  2:138389/150‑1 (MQ=255)
                                                                                                                                        aGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTg          <  2:1131110/150‑1 (MQ=255)
                                                                                                                                        aGCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTg          <  2:2938295/150‑1 (MQ=255)
                                                                                                                                         gCTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTgg         <  2:1644692/150‑1 (MQ=255)
                                                                                                                                          cTGGTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTggg        >  2:2357749/1‑150 (MQ=255)
                                                                                                                                             gTAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGt     >  2:3385338/1‑150 (MQ=255)
                                                                                                                                              tAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGtt    >  2:3254940/1‑150 (MQ=255)
                                                                                                                                              tAAATTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGtt    >  2:3255012/1‑150 (MQ=255)
                                                                                                                                                aaTTATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTAt  >  2:3605791/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATGATGAAGTTCCGGTCGCAGCACCTGTGGCCTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCCAAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTAT  >  CP009273/4035865‑4036158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑