Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,176,823 G→T D516Y (GAC→TAC)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,176,8230GT100.0% 618.9 / NA 185D516Y (GAC→TAC) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (99/86);  total (99/86)

Only 100 of 185 total aligned reads displayed.

CTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTA  >  CP009273/4176677‑4176969
                                                                                                                                                  |                                                                                                                                                  
cTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGGTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACt                                                                                                                                                 <  2:90183/150‑1 (MQ=255)
 tGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACtg                                                                                                                                                >  1:3036360/1‑150 (MQ=255)
   ttCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACtgtg                                                                                                                                              <  1:2393649/150‑1 (MQ=255)
   ttCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACtgtg                                                                                                                                              <  1:2393156/150‑1 (MQ=255)
   ttCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACtgtg                                                                                                                                              <  1:487303/150‑1 (MQ=255)
    tCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTgtgt                                                                                                                                             >  2:2230983/1‑150 (MQ=255)
        gTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAAc                                                                                                                                         <  2:2457108/150‑1 (MQ=255)
        gTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAAc                                                                                                                                         <  2:1950805/150‑1 (MQ=255)
        gTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAAc                                                                                                                                         >  2:1014904/1‑150 (MQ=255)
         tACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACg                                                                                                                                        <  2:321894/150‑1 (MQ=255)
         tACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACg                                                                                                                                        >  1:499176/1‑150 (MQ=255)
            cGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCa                                                                                                                                     <  1:1332254/150‑1 (MQ=255)
              cTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCaaa                                                                                                                                   >  2:206976/1‑150 (MQ=255)
              cTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCaaa                                                                                                                                   >  2:3195118/1‑150 (MQ=255)
               tGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAg                                                                                                                                  >  1:1973386/1‑150 (MQ=255)
                gACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAgg                                                                                                                                 <  1:379386/150‑1 (MQ=255)
                    cTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGaa                                                                                                                             <  2:2596125/150‑1 (MQ=255)
                     tGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAg                                                                                                                            >  2:408573/1‑150 (MQ=255)
                     tGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAg                                                                                                                            <  2:796356/150‑1 (MQ=255)
                      ggAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAgg                                                                                                                           <  1:49637/150‑1 (MQ=255)
                       gAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGc                                                                                                                          >  1:2369667/1‑150 (MQ=255)
                        aaGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCa                                                                                                                         >  1:1000650/1‑150 (MQ=255)
                         aGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCAt                                                                                                                        <  2:649956/150‑1 (MQ=255)
                          gCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATg                                                                                                                       <  2:2707390/150‑1 (MQ=255)
                           cccAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCTGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATgg                                                                                                                      <  2:1141871/150‑1 (MQ=255)
                             cAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTg                                                                                                                    <  1:816737/150‑1 (MQ=255)
                              aGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGc                                                                                                                   <  1:1295884/150‑1 (MQ=255)
                                cTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTg                                                                                                                 <  2:3649755/150‑1 (MQ=255)
                                  ggAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGAc                                                                                                               <  2:2688928/150‑1 (MQ=255)
                                  ggAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGAc                                                                                                               <  2:784369/150‑1 (MQ=255)
                                   gAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACt                                                                                                              <  2:3356807/150‑1 (MQ=255)
                                   gAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACt                                                                                                              <  2:2832402/150‑1 (MQ=255)
                                    aaGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTg                                                                                                             <  2:2974969/150‑1 (MQ=255)
                                     aGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAAGCATGGTGCTGACTgg                                                                                                            >  1:2166892/1‑150 (MQ=255)
                                          gTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGa                                                                                                       <  2:818889/150‑1 (MQ=255)
                                           tGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGaa                                                                                                      >  2:541987/1‑150 (MQ=255)
                                            gcgcTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGaaa                                                                                                     <  2:1328781/150‑1 (MQ=255)
                                             cgcTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaag                                                                                                    >  2:110749/1‑150 (MQ=255)
                                                tgatgaTGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaagaag                                                                                                 >  1:157091/1‑150 (MQ=255)
                                                tgatgaTGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaagaag                                                                                                 <  1:2788892/150‑1 (MQ=255)
                                                 gatgatGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCAGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGc                                                                                                <  2:3772420/150‑1 (MQ=255)
                                                      tgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAc                                                                                           >  1:3559835/1‑150 (MQ=255)
                                                      tgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAc                                                                                           >  1:975626/1‑150 (MQ=255)
                                                      tgTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAAc                                                                                           <  1:3089600/150‑1 (MQ=255)
                                                          tACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCt                                                                                       >  2:3210921/1‑150 (MQ=255)
                                                          tACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCt                                                                                       <  2:3004149/150‑1 (MQ=255)
                                                          tACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCt                                                                                       >  1:3297632/1‑150 (MQ=255)
                                                             caacaaCATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTa                                                                                    >  2:2576162/1‑150 (MQ=255)
                                                                 aacaTCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGc                                                                                >  1:2965310/1‑150 (MQ=255)
                                                                    aTCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGctct                                                                             <  1:345236/150‑1 (MQ=255)
                                                                        tGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCtctggtc                                                                         >  1:581540/1‑150 (MQ=255)
                                                                        tGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCtctggtc                                                                         >  2:3278695/1‑150 (MQ=255)
                                                                           ccccGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCtctggtctgg                                                                      >  1:2073562/1‑150 (MQ=255)
                                                                             ccGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTTTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCt                                                                    >  1:787442/1‑150 (MQ=255)
                                                                             ccGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCt                                                                    <  1:3455290/150‑1 (MQ=255)
                                                                             ccGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCt                                                                    <  1:3482899/150‑1 (MQ=255)
                                                                             ccGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCt                                                                    <  1:2244173/150‑1 (MQ=255)
                                                                              cggcgaacggcgaaCCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCtt                                                                   >  1:1906948/1‑150 (MQ=255)
                                                                                  gaacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTctct                                                               <  2:473347/150‑1 (MQ=255)
                                                                                     cggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCa                                                            >  1:2010300/1‑150 (MQ=255)
                                                                                     cggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCa                                                            >  1:3542367/1‑150 (MQ=255)
                                                                                        cgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATgc                                                         >  1:2253173/1‑150 (MQ=255)
                                                                                        cgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATgc                                                         >  1:2466663/1‑150 (MQ=255)
                                                                                         gaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATgcg                                                        <  1:2503242/150‑1 (MQ=255)
                                                                                         gaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATgcg                                                        >  1:1939532/1‑150 (MQ=255)
                                                                                            ccAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATgcgcgc                                                     >  1:224065/1‑150 (MQ=255)
                                                                                               atcatcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGtt                                                  <  1:704167/150‑1 (MQ=255)
                                                                                                 catcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTaa                                                >  2:571542/1‑150 (MQ=255)
                                                                                                 catcGTTCCGTCTCAGGACGTTGTAATGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTaa                                                >  2:3551792/1‑150 (MQ=255)
                                                                                                   tcGGTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAg                                              <  2:1236040/150‑1 (MQ=255)
                                                                                                    cGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGt                                             <  1:1241195/150‑1 (MQ=255)
                                                                                                     gTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTg                                            >  1:1033902/1‑150 (MQ=255)
                                                                                                     gTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTg                                            <  1:117911/150‑1 (MQ=255)
                                                                                                     gTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTg                                            <  2:2375598/150‑1 (MQ=255)
                                                                                                     gTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTg                                            <  1:1735255/150‑1 (MQ=255)
                                                                                                     gTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTg                                            >  1:2642125/1‑150 (MQ=255)
                                                                                                      ttCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGc                                           <  1:2790184/150‑1 (MQ=255)
                                                                                                        ccGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGt                                         >  2:1482924/1‑150 (MQ=255)
                                                                                                            ctcAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCa                                     <  1:541987/150‑1 (MQ=255)
                                                                                                            ctcAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCa                                     >  2:1532510/1‑150 (MQ=255)
                                                                                                              cAGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGTCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCAcc                                   >  2:2307282/1‑150 (MQ=255)
                                                                                                               aGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCg                                  >  2:2917062/1‑150 (MQ=255)
                                                                                                               aGGACGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCg                                  >  2:1705438/1‑150 (MQ=255)
                                                                                                                  aCGTTGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGt                               <  1:2987290/150‑1 (MQ=255)
                                                                                                                      tGTACTGGGTCTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGa                           >  2:343328/1‑150 (MQ=255)
                                                                                                                                cTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCt                 <  1:206976/150‑1 (MQ=255)
                                                                                                                                cTGTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCt                 >  2:2700450/1‑150 (MQ=255)
                                                                                                                                  gTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTaa               >  2:2362946/1‑150 (MQ=255)
                                                                                                                                  gTACTACATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTaa               >  2:2747581/1‑150 (MQ=255)
                                                                                                                                    actacATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACg             >  2:682471/1‑150 (MQ=255)
                                                                                                                                    actacATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACg             <  1:937424/150‑1 (MQ=255)
                                                                                                                                    actacATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACg             >  2:1757139/1‑150 (MQ=255)
                                                                                                                                     ctacATGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACgg            >  2:967125/1‑150 (MQ=255)
                                                                                                                                         aTGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGaa        <  1:2710868/150‑1 (MQ=255)
                                                                                                                                          tGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAAt       >  1:891004/1‑150 (MQ=255)
                                                                                                                                          tGACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCGTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAAt       <  1:426674/150‑1 (MQ=255)
                                                                                                                                           gACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAAtt      <  1:1501968/150‑1 (MQ=255)
                                                                                                                                           gACCCGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAAtt      <  2:2377258/150‑1 (MQ=255)
                                                                                                                                              ccGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATtagt   >  2:2585866/1‑150 (MQ=255)
                                                                                                                                               cGTTACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATtagta  <  2:2005670/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCAGAACGTCTGTATCGCTCTGGTCTGGCTTCTCTGCATGCGCGCGTTAAAGTGCGTATCACCGAGTATGAAAAAGATGCTAACGGTGAATTAGTA  >  CP009273/4176677‑4176969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑