Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 31,710 C→A I298I (ATC→ATA)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927331,7100CA97.7% 659.5 / NA 213I298I (ATC→ATA) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (110/98);  minor base T (0/5);  total (110/103)
Fisher's exact test for biased strand distribution p-value = 2.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

TGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTC  >  CP009273/31563‑31856
                                                                                                                                                   |                                                                                                                                                  
tGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGa                                                                                                                                                  >  1:723433/1‑150 (MQ=255)
tGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGa                                                                                                                                                  >  1:524183/1‑150 (MQ=255)
 gTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGaa                                                                                                                                                 <  2:1947463/150‑1 (MQ=255)
   cgcgCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAAt                                                                                                                                               >  1:3805559/1‑150 (MQ=255)
    gcgcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATg                                                                                                                                              <  2:1987167/150‑1 (MQ=255)
        cAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAAcc                                                                                                                                          <  2:3749548/150‑1 (MQ=255)
        cAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAAcc                                                                                                                                          >  2:2725217/1‑150 (MQ=255)
        cAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAAcc                                                                                                                                          >  2:1221561/1‑150 (MQ=255)
          gCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCa                                                                                                                                        >  1:3539155/1‑150 (MQ=255)
           cccAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAc                                                                                                                                       <  2:958304/150‑1 (MQ=255)
            ccAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcg                                                                                                                                      >  1:149364/1‑150 (MQ=255)
             cAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcgc                                                                                                                                     >  2:1662445/1‑150 (MQ=255)
             cAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcgc                                                                                                                                     >  2:446668/1‑150 (MQ=255)
              aaaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCActcg                                                                                                                                    >  1:3363550/1‑150 (MQ=255)
                 cGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCgtgt                                                                                                                                 >  2:1146715/1‑150 (MQ=255)
                 cGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCgtgt                                                                                                                                 >  2:3363231/1‑150 (MQ=255)
                 cGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCgtgt                                                                                                                                 <  1:3757108/150‑1 (MQ=255)
                     gACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCg                                                                                                                             >  2:2632650/1‑150 (MQ=255)
                       ccGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGtt                                                                                                                           <  2:3477278/150‑1 (MQ=255)
                        cGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACGCACGCGTGTCCCGttc                                                                                                                          >  2:3592591/1‑150 (MQ=255)
                         gACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttct                                                                                                                         >  2:3098410/1‑150 (MQ=255)
                         gACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttct                                                                                                                         >  2:1363898/1‑150 (MQ=255)
                         gACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGAGTGTTATAGAAATGAACCCACGCGTGTCCCGttct                                                                                                                         >  2:1728101/1‑150 (MQ=255)
                          aCAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttctt                                                                                                                        >  1:3402674/1‑150 (MQ=255)
                            aaaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCg                                                                                                                      >  2:1629231/1‑150 (MQ=255)
                            aaaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCg                                                                                                                      <  1:3128981/150‑1 (MQ=255)
                            aaaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCg                                                                                                                      <  1:2276190/150‑1 (MQ=255)
                             aaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCgg                                                                                                                     >  1:3679365/1‑150 (MQ=255)
                              aGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGgc                                                                                                                    >  2:793694/1‑150 (MQ=255)
                                aaTATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGgcgc                                                                                                                  >  1:945441/1‑150 (MQ=255)
                                 atatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCt                                                                                                                 >  1:1390758/1‑150 (MQ=255)
                                  tatCACCTCATGCGTAACGCCTCGAGGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGACAAACGGTCGTCTGATTGTTATAGAAATGACCCCACGCGTGTCCCGTTCTTCGGCGCTg                                                                                                                <  2:3697688/150‑1 (MQ=255)
                                    tCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGc                                                                                                              <  1:2426959/150‑1 (MQ=255)
                                    tCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGc                                                                                                              <  1:2040645/150‑1 (MQ=255)
                                       aaTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTc                                                                                                           <  2:2836278/150‑1 (MQ=255)
                                         tCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGa                                                                                                         <  1:447898/150‑1 (MQ=255)
                                          cATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaa                                                                                                        >  1:1893186/1‑150 (MQ=255)
                                           aTGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaaa                                                                                                       >  1:2560243/1‑150 (MQ=255)
                                            tGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAg                                                                                                      <  1:2230369/150‑1 (MQ=255)
                                              cGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCg                                                                                                    <  1:838686/150‑1 (MQ=255)
                                              cGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCg                                                                                                    >  2:251304/1‑150 (MQ=255)
                                              cGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCg                                                                                                    >  2:2804307/1‑150 (MQ=255)
                                                tAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAc                                                                                                  >  2:1290011/1‑150 (MQ=255)
                                                 aaCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                                                 <  2:3684016/150‑1 (MQ=255)
                                                 aaCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                                                 >  1:3239691/1‑150 (MQ=255)
                                                    gCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGt                                                                                              >  2:2939939/1‑150 (MQ=255)
                                                    gCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGt                                                                                              >  2:2741664/1‑150 (MQ=255)
                                                      cTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGttt                                                                                            >  2:3181357/1‑150 (MQ=255)
                                                         gATGGGGGTGCTGTGTGAAATCGGTGTTTAAACCGGTGGTTCCAACGTTCTGTTGGCGGTGAACCCTAAAAACGGTTGTTTGTTTGTTATATAAATGAACCCCCGGGTGTCCCGGTCTTGGGGGCTGGCGTCGAAAGCGACCGGTTTccc                                                                                         <  1:3574137/150‑1 (MQ=255)
                                                          aTGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCg                                                                                        >  1:623089/1‑150 (MQ=255)
                                                          aTGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCg                                                                                        >  2:1498092/1‑150 (MQ=255)
                                                            ggCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGAt                                                                                      >  2:2850082/1‑150 (MQ=255)
                                                             gCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGAtt                                                                                     <  2:3021190/150‑1 (MQ=255)
                                                                     gcGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTTAACCCTAAAAACGGTCGTCTGATTGTTATATAAATTAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGt                                                                             <  1:1244802/150‑1 (MQ=255)
                                                                       gttaaaTCGGTGTTTAAACCGGTGTTTCAAATGTTCTGTTTGTGGTGAACCCTAATAATGGTCGTTTGTTTGTTATTTAATTGAACCCACGCGTGTCCCGTTCTTCGGCGGTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTgg                                                                           <  1:534425/147‑1 (MQ=255)
                                                                          aaaTCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTggcgg                                                                        >  2:3213338/1‑150 (MQ=255)
                                                                          aaaTCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTggcgg                                                                        >  2:2480533/1‑150 (MQ=255)
                                                                                gtgTTTAAACGGGGGGTTCCAATGTTCTGTTTGCGGTTAACCCTAAAAACGGTCGTTTGATTGTTATATAAATTAACCCACGCGGGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAAc                                                                  <  1:2116420/148‑1 (MQ=255)
                                                                                 cGTTGAACCCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACt                                                                 <  2:2325915/150‑1 (MQ=255)
                                                                                 cGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACt                                                                 >  2:1135620/1‑150 (MQ=255)
                                                                                  gTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTg                                                                >  2:1470598/1‑150 (MQ=255)
                                                                                     gNAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCg                                                             >  1:3150369/1‑150 (MQ=255)
                                                                                     gAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCg                                                             >  2:1504156/1‑150 (MQ=255)
                                                                                       aaCCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGt                                                           >  2:3687125/1‑150 (MQ=255)
                                                                                        actggtggtTCCAATGTTCTGTTTGCGGTTAACCCTAAAAATGGTTGTCTGTTTGTTATATAATTGAACCCCCGCGTGTCCGGTTCTTCGGCGCTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTg                                                          <  1:3272895/147‑1 (MQ=255)
                                                                                         ccGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTgg                                                         <  2:2794864/150‑1 (MQ=255)
                                                                                         ccGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTgg                                                         <  2:760180/150‑1 (MQ=255)
                                                                                          cGGTGGTTCCAACGTTCTGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGACCCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTggg                                                        <  1:3077222/150‑1 (MQ=255)
                                                                                          cGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTggg                                                        >  1:822922/1‑150 (MQ=255)
                                                                                          cGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTggg                                                        >  1:1314310/1‑150 (MQ=255)
                                                                                           ggtggtTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGt                                                       >  1:3628433/1‑150 (MQ=255)
                                                                                            gtggtTCCAACTTTCAGTTTGCGGTTAACCCTAAAAACGGTCGTTTTATTGTTATAGAATTTAACCCACGCGGGTCCCGTTTTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGtt                                                      <  1:1683349/150‑1 (MQ=255)
                                                                                             tggtTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTa                                                     >  2:250785/1‑150 (MQ=255)
                                                                                              ggtTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTac                                                    >  2:1246790/1‑150 (MQ=255)
                                                                                              ggtTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTac                                                    >  1:2702645/1‑150 (MQ=255)
                                                                                               gtTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTaca                                                   <  1:2804264/150‑1 (MQ=255)
                                                                                                  ccAATGTTCTGTTTGCGGTGAACCCGAAAAACGGTCGTCTTTTTGTTATAGAAATTAACCCACGCGGGTCCCGTTTTTCGGCGCTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACAccc                                                <  1:716306/150‑1 (MQ=255)
                                                                                                  ccAACGTACAGTTTGCGGTTAACCCTAAAAACGGTCGTCTGATTGTTATATAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACAccc                                                <  1:2502962/150‑1 (MQ=255)
                                                                                                   cAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGGGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCt                                               <  2:3539187/150‑1 (MQ=255)
                                                                                                       gTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcgac                                           >  1:2207593/1‑150 (MQ=255)
                                                                                                          ctgTTTGCGGTGAACCTGAAAATGGGTTGTTTGTTTTTTATTTAATTTAACCCACGGGGGGCCCGTTTTTCGGCGTGGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGaa                                        <  1:1015523/148‑1 (MQ=255)
                                                                                                              ttGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGACCtga                                    >  1:2970528/1‑150 (MQ=255)
                                                                                                               tGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtgat                                   <  2:3162577/150‑1 (MQ=255)
                                                                                                                  ggTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGaa                                >  1:1344797/1‑150 (MQ=255)
                                                                                                                   gTGAACCCGAAAAACGGTCGTCTGATTGTTATATAAATGAACCCACGTGTGTCCCGTTCTTCGGCGCTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAAc                               <  1:2000489/150‑1 (MQ=255)
                                                                                                                   gTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAAc                               >  1:3451250/1‑150 (MQ=255)
                                                                                                                     gAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGa                             >  1:2666641/1‑150 (MQ=255)
                                                                                                                           gAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCAc                       >  1:2737097/1‑150 (MQ=255)
                                                                                                                           gAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTGTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCAc                       <  2:1718648/150‑1 (MQ=255)
                                                                                                                             aaaaCGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTg                     >  2:508237/1‑150 (MQ=255)
                                                                                                                               aaCGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGc                   >  2:845712/1‑150 (MQ=255)
                                                                                                                                aCGGTCGTCTGATTGTTATAGAAATGAACCCACGCGGGTCCCGTTTTTCGGCGCTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCg                  <  1:2201303/150‑1 (MQ=255)
                                                                                                                                  ggTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGa                <  2:1379579/150‑1 (MQ=255)
                                                                                                                                  ggTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGa                >  1:2437608/1‑150 (MQ=255)
                                                                                                                                       tcTGATTGTTATAGAAATGAACCCACGCGGGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTAc           <  1:2831481/150‑1 (MQ=255)
                                                                                                                                          gATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTcc        >  2:2082238/1‑150 (MQ=255)
                                                                                                                                           aTTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCg       >  2:2312470/1‑150 (MQ=255)
                                                                                                                                            ttGTTATAGAAATGAACCCACGCGGGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCgg      <  2:385331/150‑1 (MQ=255)
                                                                                                                                              gTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGcc    <  2:3261111/150‑1 (MQ=255)
                                                                                                                                               ttATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGcct   >  2:2779270/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTC  >  CP009273/31563‑31856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑