Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,178,969 G→T R1231L (CGT→CTT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,178,9690GT100.0% 655.1 / NA 199R1231L (CGT→CTT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (95/104);  total (95/104)

Only 100 of 204 total aligned reads displayed.

GTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGG  >  CP009273/4178822‑4179115
                                                                                                                                                   |                                                                                                                                                  
gTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCttt                                                                                                                                                  <  2:3326220/150‑1 (MQ=255)
gTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCttt                                                                                                                                                  <  2:1464595/150‑1 (MQ=255)
 tAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTa                                                                                                                                                 <  1:1688865/150‑1 (MQ=255)
   gCGATCCGGACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACa                                                                                                                                               <  1:668461/150‑1 (MQ=255)
    cGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACAt                                                                                                                                              >  1:20790/1‑150 (MQ=255)
         cGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTa                                                                                                                                         <  1:187045/150‑1 (MQ=255)
           taagAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGAAATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAAc                                                                                                                                       <  1:2206245/147‑1 (MQ=255)
           tACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAAc                                                                                                                                       >  2:1955010/1‑150 (MQ=255)
            aCGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACg                                                                                                                                      >  2:762348/1‑150 (MQ=255)
              gAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGaa                                                                                                                                    >  2:3576391/1‑150 (MQ=255)
              gAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGaa                                                                                                                                    <  2:2653698/150‑1 (MQ=255)
               aaGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAg                                                                                                                                   <  2:1740423/150‑1 (MQ=255)
               aaGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAg                                                                                                                                   <  1:2328992/150‑1 (MQ=255)
                  agaTGATTCCGAAATGGCGTCAGCTCATCGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTAc                                                                                                                                <  1:3241246/150‑1 (MQ=255)
                   gatgatTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACa                                                                                                                               >  2:1853621/1‑150 (MQ=255)
                    atgatTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAg                                                                                                                              <  1:3363513/150‑1 (MQ=255)
                      gatTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGa                                                                                                                            >  1:1889612/1‑150 (MQ=255)
                       atTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGAc                                                                                                                           >  2:2921028/1‑150 (MQ=255)
                        ttCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACg                                                                                                                          >  2:1261627/1‑150 (MQ=255)
                         tCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGt                                                                                                                         >  1:1005143/1‑150 (MQ=255)
                         tCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGt                                                                                                                         <  2:2256074/150‑1 (MQ=255)
                           cGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGtat                                                                                                                       >  1:1810131/1‑150 (MQ=255)
                              aaTGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATAcc                                                                                                                    <  2:3526626/150‑1 (MQ=255)
                              aaTGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATAcc                                                                                                                    >  1:2733991/1‑150 (MQ=255)
                              aaTGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATAcc                                                                                                                    <  2:1674060/150‑1 (MQ=255)
                                tGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGt                                                                                                                  >  2:3681764/1‑150 (MQ=255)
                                tGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGt                                                                                                                  >  2:1786349/1‑150 (MQ=255)
                                      cAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAg                                                                                                            >  1:477674/1‑150 (MQ=255)
                                       aGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAgg                                                                                                           <  1:2159899/150‑1 (MQ=255)
                                       aGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAgg                                                                                                           <  2:3263110/150‑1 (MQ=255)
                                        gCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAggg                                                                                                          >  2:3141606/1‑150 (MQ=255)
                                         cTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGc                                                                                                         >  2:3761681/1‑150 (MQ=255)
                                         cTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGc                                                                                                         >  1:1521691/1‑150 (MQ=255)
                                          tCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCg                                                                                                        <  1:2478433/150‑1 (MQ=255)
                                          tCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCg                                                                                                        >  1:3275431/1‑150 (MQ=255)
                                             aCGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTa                                                                                                     <  1:841135/150‑1 (MQ=255)
                                              cGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTaa                                                                                                    <  1:790692/150‑1 (MQ=255)
                                              cGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTaa                                                                                                    <  2:2432069/150‑1 (MQ=255)
                                              cGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTaa                                                                                                    <  2:2433017/150‑1 (MQ=255)
                                               gtgtTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAg                                                                                                   >  2:2472295/1‑150 (MQ=255)
                                                tgtTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGa                                                                                                  <  2:2861220/150‑1 (MQ=255)
                                                 gtTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGAt                                                                                                 >  2:411980/1‑150 (MQ=255)
                                                   tCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTa                                                                                               <  2:741167/150‑1 (MQ=255)
                                                    cGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTaa                                                                                              >  2:3079086/1‑150 (MQ=255)
                                                      aaGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACg                                                                                            >  2:1881734/1‑150 (MQ=255)
                                                         gTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATa                                                                                         <  2:518036/150‑1 (MQ=255)
                                                           gAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATaaa                                                                                       >  2:5747/1‑150 (MQ=255)
                                                            aaCGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAac                                                                                      <  2:774128/150‑1 (MQ=255)
                                                                  tAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCg                                                                                >  2:430248/1‑150 (MQ=255)
                                                                  tAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCg                                                                                <  2:1968665/150‑1 (MQ=255)
                                                                   aGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGa                                                                               >  2:1085604/1‑150 (MQ=255)
                                                                      aCGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGt                                                                            <  2:1203857/150‑1 (MQ=255)
                                                                       cGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGtt                                                                           <  2:1003917/150‑1 (MQ=255)
                                                                       cGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGtt                                                                           <  1:3722334/150‑1 (MQ=255)
                                                                          ggtgACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTAtc                                                                        >  1:2885078/1‑150 (MQ=255)
                                                                           gtgACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTAtcg                                                                       >  1:1395331/1‑150 (MQ=255)
                                                                           gtgACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTAtcg                                                                       <  1:550265/150‑1 (MQ=255)
                                                                                   aTTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAg                                                               >  1:1080160/1‑150 (MQ=255)
                                                                                   aTTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAg                                                               >  1:2981836/1‑150 (MQ=255)
                                                                                    tttCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGa                                                              <  1:3491051/150‑1 (MQ=255)
                                                                                       ccGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGAtgc                                                           <  1:2951572/150‑1 (MQ=255)
                                                                                        cGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGAtgct                                                          <  2:2392429/150‑1 (MQ=255)
                                                                                            ggTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGt                                                      >  2:353950/1‑150 (MQ=255)
                                                                                            ggTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGt                                                      >  1:313949/1‑150 (MQ=255)
                                                                                             gTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTa                                                     <  1:148506/150‑1 (MQ=255)
                                                                                             gTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTa                                                     <  1:2059754/150‑1 (MQ=255)
                                                                                               ccGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTaaa                                                   <  2:3459480/150‑1 (MQ=255)
                                                                                                 ggAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTTAAGc                                                 <  1:1034620/150‑1 (MQ=255)
                                                                                                   aaGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTa                                               <  2:2729685/150‑1 (MQ=255)
                                                                                                         cgcACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCg                                         <  1:1955010/150‑1 (MQ=255)
                                                                                                         cgcACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCg                                         >  2:1211597/1‑150 (MQ=255)
                                                                                                           cACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGtt                                       >  2:3722186/1‑150 (MQ=255)
                                                                                                           cACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGtt                                       >  2:932796/1‑150 (MQ=255)
                                                                                                              gACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAc                                    >  2:1483515/1‑150 (MQ=255)
                                                                                                               aCATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAcg                                   >  2:1662240/1‑150 (MQ=255)
                                                                                                               aCATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAcg                                   <  2:2855129/150‑1 (MQ=255)
                                                                                                                taTTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAcgc                                  >  2:524098/2‑150 (MQ=255)
                                                                                                                   tctgcgtctgcgTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCggg                               <  1:1853621/150‑1 (MQ=255)
                                                                                                                    ctgcgtctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGt                              >  1:125459/1‑150 (MQ=255)
                                                                                                                     tgcgtctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTTCGTAAAGCTACCATCGTTAACGCGGGTa                             >  1:3213735/1‑150 (MQ=255)
                                                                                                                      gcgtctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAg                            <  1:3696601/150‑1 (MQ=255)
                                                                                                                      gcgtctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAg                            <  1:1904324/150‑1 (MQ=255)
                                                                                                                       cgtctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGc                           >  1:630557/1‑150 (MQ=255)
                                                                                                                        gtctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCt                          <  1:1796683/150‑1 (MQ=255)
                                                                                                                          ctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTcc                        >  2:3569195/1‑150 (MQ=255)
                                                                                                                             cgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGAc                     >  1:2443914/1‑150 (MQ=255)
                                                                                                                             cgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGAc                     <  1:3287514/150‑1 (MQ=255)
                                                                                                                             cgtGGTGTTCATGCTGTTACTATTTACATCGTTAACGAATTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCTTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGAc                     <  1:1044798/150‑1 (MQ=255)
                                                                                                                                ggtgTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTc                  >  1:387940/1‑150 (MQ=255)
                                                                                                                                  tgtTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCt                <  1:3681764/150‑1 (MQ=255)
                                                                                                                                    ttCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTgg              <  1:1662240/150‑1 (MQ=255)
                                                                                                                                      cATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGaa            >  2:1387106/1‑150 (MQ=255)
                                                                                                                                        tGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATAGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAgg          <  1:1434697/150‑1 (MQ=255)
                                                                                                                                         gCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGc         <  2:1521691/150‑1 (MQ=255)
                                                                                                                                          cTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCg        >  2:765243/1‑150 (MQ=255)
                                                                                                                                           tGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGa       >  1:1920240/1‑150 (MQ=255)
                                                                                                                                           tGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGa       <  1:960427/150‑1 (MQ=255)
                                                                                                                                                ctctTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAgg  <  1:353529/150‑1 (MQ=255)
                                                                                                                                                ctctTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAgg  <  1:353950/150‑1 (MQ=255)
                                                                                                                                                ctctTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAgg  >  1:1751081/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGG  >  CP009273/4178822‑4179115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑