BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,193,578	G→T	D111Y (GAT→TAT)	zraR →	fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,193,578	0	G	T	100.0%	850.9 / NA	257	D111Y (GAT→TAT)	zraR	fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (137/120); total (137/120)

Only 100 of 257 total aligned reads displayed.

GACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCGCG  >  CP009273/4193434‑4193724

    gCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTAc                                                                                                                                           >  2:1130728/1‑150 (MQ=255)

    gCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTAc                                                                                                                                           <  2:2942951/150‑1 (MQ=255)

    gCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTAc                                                                                                                                           >  2:2414447/1‑150 (MQ=255)

      aTCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAg                                                                                                                                         >  2:635276/1‑150 (MQ=255)

      aTCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAg                                                                                                                                         >  2:1363239/1‑150 (MQ=255)

        tgCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGc                                                                                                                                       >  2:938547/2‑150 (MQ=255)

        tgCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGc                                                                                                                                       >  2:2903669/2‑150 (MQ=255)

        cGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGc                                                                                                                                       >  1:407222/1‑150 (MQ=255)

                tGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTgg                                                                                                                               >  1:2256571/1‑150 (MQ=255)

                  aaaGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGaa                                                                                                                             >  1:2700858/1‑150 (MQ=255)

                     gagaTCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGaaaaa                                                                                                                          >  1:1026854/1‑150 (MQ=255)

                     gagaTCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGaaaaa                                                                                                                          <  1:2292810/150‑1 (MQ=255)

                            aaGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTcg                                                                                                                   >  2:1592215/1‑150 (MQ=255)

                             aGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTcgc                                                                                                                  <  1:2215059/150‑1 (MQ=255)

                                gTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCa                                                                                                               >  2:1506940/1‑150 (MQ=255)

                                 ttAAACCCGGCAAGTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCAt                                                                                                              <  1:46572/150‑1 (MQ=255)

                                  tAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATa                                                                                                             >  2:2226178/1‑150 (MQ=255)

                                  tAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATa                                                                                                             <  1:775993/150‑1 (MQ=255)

                                   aaaCCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATAc                                                                                                            >  1:752179/1‑150 (MQ=255)

                                    aaCCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACg                                                                                                           <  2:2095781/150‑1 (MQ=255)

                                    aaCCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACg                                                                                                           >  2:2110115/1‑150 (MQ=255)

                                       ccGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGcac                                                                                                        <  2:2385173/150‑1 (MQ=255)

                                       ccGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGcac                                                                                                        >  2:3225515/1‑150 (MQ=255)

                                        cGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGcaca                                                                                                       >  2:711627/1‑150 (MQ=255)

                                            aaTTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTAt                                                                                                   <  2:1588605/150‑1 (MQ=255)

                                            aaTTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTAt                                                                                                   <  2:3442802/150‑1 (MQ=255)

                                             aTTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTAtt                                                                                                  >  1:325947/1‑150 (MQ=255)

                                               tCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGa                                                                                                >  2:1907651/1‑150 (MQ=255)

                                                ccGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGAt                                                                                               >  1:3701804/1‑150 (MQ=255)

                                                  ggTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGc                                                                                             <  2:547364/150‑1 (MQ=255)

                                                   gTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGGTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCt                                                                                            <  2:891231/150‑1 (MQ=255)

                                                   gTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCt                                                                                            >  2:1490621/1‑150 (MQ=255)

                                                    tGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTg                                                                                           <  1:2559008/150‑1 (MQ=255)

                                                      cTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGaa                                                                                         >  1:832849/1‑150 (MQ=255)

                                                      cTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGaa                                                                                         >  2:177966/1‑150 (MQ=255)

                                                      cTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGaa                                                                                         >  1:127461/1‑150 (MQ=255)

                                                          ttATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAacac                                                                                     <  1:1314599/150‑1 (MQ=255)

                                                           tATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACAcc                                                                                    >  1:45743/1‑150 (MQ=255)

                                                            aTGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCt                                                                                   >  1:2302359/1‑150 (MQ=255)

                                                              gACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCCCGCGCATACGCACAGTATTGATGCTGAAACACCTGc                                                                                 <  1:3059498/150‑1 (MQ=255)

                                                                   cGTACTCCGGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGa                                                                            <  1:3491635/150‑1 (MQ=255)

                                                                   cGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGa                                                                            >  2:3340287/1‑150 (MQ=255)

                                                                     tACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACt                                                                          <  1:3575765/150‑1 (MQ=255)

                                                                       cTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTgc                                                                        >  1:2822089/1‑150 (MQ=255)

                                                                           aGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTgccagc                                                                    >  2:3084729/1‑150 (MQ=255)

                                                                              gTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGccagccag                                                                 <  2:3453295/150‑1 (MQ=255)

                                                                              gTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGccagccag                                                                 >  2:3131946/1‑150 (MQ=255)

                                                                                cGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGtt                                                               >  2:3063465/1‑150 (MQ=255)

                                                                                 gagaCGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTc                                                              >  1:1958602/1‑150 (MQ=255)

                                                                                 gagaCGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTc                                                              >  2:3773111/1‑150 (MQ=255)

                                                                                 gagaCGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTc                                                              <  1:1291489/150‑1 (MQ=255)

                                                                                 gagaCGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTc                                                              >  1:3715157/1‑150 (MQ=255)

                                                                                   gaCGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCAGCGGTGACTGCCAGCCAGTTCgg                                                            <  2:2584493/150‑1 (MQ=255)

                                                                                    aCGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGt                                                           <  1:2268358/150‑1 (MQ=255)

                                                                                    aCGGCGGTAGAGGCACCGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGt                                                           <  1:1100545/150‑1 (MQ=255)

                                                                                        cggTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGGGACTGCCAGCCAGGTCGGGATgg                                                       >  2:1002564/1‑150 (MQ=255)

                                                                                           tAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCg                                                    <  1:1588060/150‑1 (MQ=255)

                                                                                           tAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCg                                                    <  1:635276/150‑1 (MQ=255)

                                                                                            agagGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCgg                                                   >  2:1683487/1‑150 (MQ=255)

                                                                                             gagGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGt                                                  <  2:2803617/150‑1 (MQ=255)

                                                                                             gagGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGt                                                  >  1:355872/1‑150 (MQ=255)

                                                                                             gagGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGt                                                  >  2:2081780/1‑150 (MQ=255)

                                                                                             gagGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCAACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGAATTGATGCTGAAACACCTGCGGTTACTGCCAGCCAGATCGGTATGGTCTGt                                                  >  1:289903/1‑150 (MQ=255)

                                                                                              agGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTa                                                 >  2:79143/1‑150 (MQ=255)

                                                                                              agGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTa                                                 >  1:1201196/1‑150 (MQ=255)

                                                                                              agGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTa                                                 <  1:353611/150‑1 (MQ=255)

                                                                                                 cACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTaaaa                                              <  1:1065811/150‑1 (MQ=255)

                                                                                                   cTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGc                                            <  1:159523/150‑1 (MQ=255)

                                                                                                    tGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGcc                                           <  1:3696264/150‑1 (MQ=255)

                                                                                                    tGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGcc                                           >  2:2951603/1‑150 (MQ=255)

                                                                                                     gAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGccc                                          <  1:937319/150‑1 (MQ=255)

                                                                                                     gAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGccc                                          <  2:1209842/150‑1 (MQ=255)

                                                                                                         aCTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTACTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCg                                      >  2:2720111/1‑150 (MQ=255)

                                                                                                           tGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGAt                                    >  1:2692861/1‑150 (MQ=255)

                                                                                                            ggggCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATg                                   <  2:2504397/150‑1 (MQ=255)

                                                                                                            ggggCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATg                                   <  2:3804768/150‑1 (MQ=255)

                                                                                                             gggCGCTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGc                                  <  1:1524416/150‑1 (MQ=255)

                                                                                                                  cTGGATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGCATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAacac                             >  2:3379067/1‑150 (MQ=255)

                                                                                                                     gATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTg                          >  2:3046112/1‑150 (MQ=255)

                                                                                                                     gATTATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTg                          >  1:2481954/1‑150 (MQ=255)

                                                                                                                        tATCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTc                       <  1:3457021/150‑1 (MQ=255)

                                                                                                                         aTCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCa                      <  1:1804787/150‑1 (MQ=255)

                                                                                                                         aTCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCa                      >  2:498586/1‑150 (MQ=255)

                                                                                                                         aTCTCATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCa                      >  2:2263622/1‑150 (MQ=255)

                                                                                                                          tctcATCAAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAg                     >  2:2541644/1‑150 (MQ=255)

                                                                                                                            tcatcaAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTg                   <  2:1309250/150‑1 (MQ=255)

                                                                                                                            tcatcaAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTg                   >  1:720681/1‑150 (MQ=255)

                                                                                                                               tcaAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGaaa                <  1:2414447/150‑1 (MQ=255)

                                                                                                                               tcaAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGaaa                <  1:1130728/150‑1 (MQ=255)

                                                                                                                                caAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAAt               >  1:1439278/1‑150 (MQ=255)

                                                                                                                                caAGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAAt               <  1:1491402/150‑1 (MQ=255)

                                                                                                                                 aaGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATc              <  1:2003418/150‑1 (MQ=255)

                                                                                                                                 aaGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATc              <  1:2923025/150‑1 (MQ=255)

                                                                                                                                 aaGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATc              <  1:385966/150‑1 (MQ=255)

                                                                                                                                  aGCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCg             >  2:2062881/1‑150 (MQ=255)

                                                                                                                                   gCCGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGc            <  1:376530/150‑1 (MQ=255)

                                                                                                                                     cGCTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGccc          >  2:3801520/1‑150 (MQ=255)

                                                                                                                                       cTGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCtc        <  2:127461/150‑1 (MQ=255)

                                                                                                                                        tGGATTTCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCtcg       <  1:711627/150‑1 (MQ=255)

                                                                                                                                             ttCTATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTcgcg  <  1:2110115/150‑1 (MQ=255)

GACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCCGCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAAGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGGTGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCGCG  >  CP009273/4193434‑4193724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`