BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,211,803	G→A	P205S (CCT→TCT)	arpA ←	ankyrin repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,211,803	0	G	A	99.6%	799.0 / NA	240	P205S (CCT→TCT)	arpA	ankyrin repeat protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (117/122); minor base T (0/1); total (117/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

TCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGGACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTC  >  CP009273/4211656‑4211949

tCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAAc                                                                                                                                                  <  2:1818762/150‑1 (MQ=255)

tCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAAc                                                                                                                                                  <  2:453204/150‑1 (MQ=255)

  tttATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACgg                                                                                                                                                <  2:3602753/150‑1 (MQ=255)

    tATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGTCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCt                                                                                                                                              >  2:2004014/1‑150 (MQ=255)

     aTTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTg                                                                                                                                             >  1:3764126/1‑150 (MQ=255)

           aGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAAcc                                                                                                                                       >  2:1373544/1‑150 (MQ=255)

              aggACCGTCAAATCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAAc                                                                                                                                    <  2:1638384/149‑1 (MQ=255)

               ggACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACg                                                                                                                                   <  2:1542661/150‑1 (MQ=255)

               ggACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACg                                                                                                                                   <  1:788316/150‑1 (MQ=255)

               ggACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACg                                                                                                                                   >  2:2420871/1‑150 (MQ=255)

                  ccGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCa                                                                                                                                <  1:1775813/150‑1 (MQ=255)

                     tCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGACACCAACGCCAAAg                                                                                                                             >  2:2042507/1‑150 (MQ=255)

                       aaaGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTc                                                                                                                           >  1:1056153/1‑150 (MQ=255)

                       aaaGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTc                                                                                                                           >  2:186106/1‑150 (MQ=255)

                        aaGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGACGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCt                                                                                                                          <  2:2404566/150‑1 (MQ=255)

                           cATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGt                                                                                                                       <  2:3304793/150‑1 (MQ=255)

                             tGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGttt                                                                                                                     <  2:3073013/150‑1 (MQ=255)

                             tGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGttt                                                                                                                     <  1:102394/150‑1 (MQ=255)

                             tGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGttt                                                                                                                     >  2:1728908/1‑150 (MQ=255)

                                ttATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTg                                                                                                                  <  2:271610/150‑1 (MQ=255)

                                      accaGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCAtttt                                                                                                            <  1:2452167/150‑1 (MQ=255)

                                       ccaGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCAttttt                                                                                                           <  2:1418223/150‑1 (MQ=255)

                                       ccaGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCAttttt                                                                                                           <  2:3468990/150‑1 (MQ=255)

                                        caGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCAtttttt                                                                                                          <  2:196464/150‑1 (MQ=255)

                                        caGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCAtttttt                                                                                                          >  2:2955653/1‑150 (MQ=255)

                                         aGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTttg                                                                                                         >  2:3350136/1‑150 (MQ=255)

                                          gTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTttgt                                                                                                        <  1:1102679/150‑1 (MQ=255)

                                          gTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTttgt                                                                                                        <  1:1692717/150‑1 (MQ=255)

                                            aaTATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTttgttg                                                                                                      >  2:3040734/1‑150 (MQ=255)

                                                   cTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCg                                                                                               <  1:3785117/150‑1 (MQ=255)

                                                    tGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGt                                                                                              <  2:2020262/150‑1 (MQ=255)

                                                          aaCACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGaaa                                                                                        >  1:3072268/1‑150 (MQ=255)

                                                          aaCACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGaaa                                                                                        >  1:129116/1‑150 (MQ=255)

                                                           acacTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAAt                                                                                       >  2:2452214/1‑150 (MQ=255)

                                                              cTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAAtttt                                                                                    <  1:1857847/150‑1 (MQ=255)

                                                               tGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAAttttt                                                                                   >  1:205249/1‑150 (MQ=255)

                                                               tGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAAttttt                                                                                   >  1:555944/1‑150 (MQ=255)

                                                                gATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGGGTGAGAAATTTTTc                                                                                  >  2:268497/1‑150 (MQ=255)

                                                                gATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTc                                                                                  >  2:2581613/1‑150 (MQ=255)

                                                                 aTGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCa                                                                                 <  1:921190/150‑1 (MQ=255)

                                                                  tGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAg                                                                                >  1:114209/1‑150 (MQ=255)

                                                                   gCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGc                                                                               <  1:3498245/150‑1 (MQ=255)

                                                                     cATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTg                                                                             <  2:427349/150‑1 (MQ=255)

                                                                      aTTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGa                                                                            >  2:1226333/1‑150 (MQ=255)

                                                                        ttGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAag                                                                          >  1:3138556/1‑150 (MQ=255)

                                                                          gATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAagag                                                                        <  2:1682433/150‑1 (MQ=255)

                                                                                aTCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAatca                                                                  >  1:446787/1‑150 (MQ=255)

                                                                                aTCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAatca                                                                  >  1:2066949/1‑150 (MQ=255)

                                                                                  cTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAatcatc                                                                <  1:2592864/150‑1 (MQ=255)

                                                                                  cTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAatcatc                                                                >  2:2318009/1‑150 (MQ=255)

                                                                                  cTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAatcatc                                                                >  1:2190628/1‑150 (MQ=255)

                                                                                   ttGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCt                                                               <  1:2710589/150‑1 (MQ=255)

                                                                                     gTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTg                                                             <  2:638426/150‑1 (MQ=255)

                                                                                       cAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGAt                                                           <  1:2386083/150‑1 (MQ=255)

                                                                                         aaaTATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATgg                                                         >  1:2435025/1‑150 (MQ=255)

                                                                                           atatGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATgggg                                                       <  2:2332003/150‑1 (MQ=255)

                                                                                           atatGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATgggg                                                       >  1:1355194/1‑150 (MQ=255)

                                                                                           atatGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATgggg                                                       <  2:1667712/150‑1 (MQ=255)

                                                                                           atatGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATgggg                                                       <  1:1231235/150‑1 (MQ=255)

                                                                                              tGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGttt                                                    <  1:1536562/150‑1 (MQ=255)

                                                                                               gCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTg                                                   <  1:3088113/150‑1 (MQ=255)

                                                                                                 tttCATAACTAGCCCGAAATCACCGCAGGCATCAACGCAACGCCCCTCATATCGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTa                                                 <  1:2001075/150‑1 (MQ=255)

                                                                                                 tttCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTa                                                 >  2:1466368/1‑150 (MQ=255)

                                                                                                   tCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAAt                                               <  2:2105740/150‑1 (MQ=255)

                                                                                                     aTAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCa                                             >  2:1315468/1‑150 (MQ=255)

                                                                                                      tAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCaa                                            >  1:2784988/1‑150 (MQ=255)

                                                                                                       aaCTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAAc                                           >  1:1913443/1‑150 (MQ=255)

                                                                                                        aCTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACa                                          <  2:690477/150‑1 (MQ=255)

                                                                                                        aCTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACa                                          <  1:3350136/150‑1 (MQ=255)

                                                                                                        aCTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACa                                          >  1:48879/1‑150 (MQ=255)

                                                                                                         cTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACAt                                         >  1:3205162/1‑150 (MQ=255)

                                                                                                         cTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACAt                                         >  1:622351/1‑150 (MQ=255)

                                                                                                          tAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACAtt                                        <  1:1373544/150‑1 (MQ=255)

                                                                                                           aGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTc                                       >  1:1470281/1‑150 (MQ=255)

                                                                                                            gCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCt                                      <  1:3784672/150‑1 (MQ=255)

                                                                                                             cccGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTg                                     >  2:510573/1‑150 (MQ=255)

                                                                                                               cGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGAt                                   <  1:1412477/150‑1 (MQ=255)

                                                                                                                gAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATa                                  >  2:1788036/1‑150 (MQ=255)

                                                                                                                gAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATa                                  >  2:2505108/1‑150 (MQ=255)

                                                                                                                 aaaTCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATaa                                 >  1:991466/1‑150 (MQ=255)

                                                                                                                 aaaTCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATaa                                 >  1:2616533/1‑150 (MQ=255)

                                                                                                                  aaTCACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATaaa                                >  1:2254761/1‑150 (MQ=255)

                                                                                                                     cACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAatat                             >  2:3789805/1‑150 (MQ=255)

                                                                                                                     cACCGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAatat                             <  1:2912688/150‑1 (MQ=255)

                                                                                                                        cGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCAt                          >  2:3372558/1‑150 (MQ=255)

                                                                                                                        cGCAGGCAGCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCAt                          <  1:1180667/150‑1 (MQ=255)

                                                                                                                           aggcagCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTaa                       <  2:3764126/150‑1 (MQ=255)

                                                                                                                           aggcagCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTaa                       <  2:247295/150‑1 (MQ=255)

                                                                                                                            ggcagCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAat                      <  2:2130060/150‑1 (MQ=255)

                                                                                                                            ggcagCAACGCAACGCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAat                      <  2:1056153/150‑1 (MQ=255)

                                                                                                                                 caacgcaacgCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATAtt                 <  1:1693547/150‑1 (MQ=255)

                                                                                                                                 caacgcaacgCCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATAtt                 >  1:1557805/1‑150 (MQ=255)

                                                                                                                                  aacgcaacgcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATtg                <  2:404861/150‑1 (MQ=255)

                                                                                                                                      caacgcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTgca            <  1:1826053/150‑1 (MQ=255)

                                                                                                                                       aacgcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTgcag           >  2:2212353/1‑150 (MQ=255)

                                                                                                                                         cgcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTgcagca         <  1:3576123/150‑1 (MQ=255)

                                                                                                                                          gcCCCTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCaa        >  2:3081261/1‑150 (MQ=255)

                                                                                                                                            cccTCCGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAAtg      <  1:2420871/150‑1 (MQ=255)

                                                                                                                                             ccTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAAtgt     >  2:1145840/1‑150 (MQ=255)

                                                                                                                                              cTCAGAACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTgcag           >  1:680843/1‑143 (MQ=255)

TCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGGACGGCTGGAAACCAACGCCAAAGTCTGGTTTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTC  >  CP009273/4211656‑4211949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`