Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,255,009 A→T V256V (GTA→GTT)  dnaB → replicative DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,255,0090AT100.0% 711.1 / NA 210V256V (GTA→GTT) dnaBreplicative DNA helicase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (105/105);  total (105/105)

Only 100 of 210 total aligned reads displayed.

AACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTACTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTATTTTGCT  >  CP009273/4254863‑4255155
                                                                                                                                                  |                                                                                                                                                  
aaCACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCtt                                                                                                                                                 <  1:1375754/150‑1 (MQ=255)
 acacCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTa                                                                                                                                                >  1:2085204/1‑150 (MQ=255)
 acacCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTa                                                                                                                                                >  2:1258814/1‑150 (MQ=255)
  cacCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAt                                                                                                                                               >  1:522163/1‑150 (MQ=255)
     cGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtctt                                                                                                                                            <  2:445243/150‑1 (MQ=255)
        ttATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTtctc                                                                                                                                         <  2:2472044/150‑1 (MQ=255)
             aCGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTgg                                                                                                                                    <  1:2192910/150‑1 (MQ=255)
              cGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGga                                                                                                                                   >  1:3135023/1‑150 (MQ=255)
               gATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGgag                                                                                                                                  >  1:2304624/1‑150 (MQ=255)
                  ctcAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATg                                                                                                                               <  1:1096835/150‑1 (MQ=255)
                      acaaAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCAt                                                                                                                           <  2:2819226/150‑1 (MQ=255)
                      acaaAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCAt                                                                                                                           >  2:3796614/1‑150 (MQ=255)
                         aaaaaaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAg                                                                                                                        >  2:3329885/1‑150 (MQ=255)
                         aaaaaaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAg                                                                                                                        >  1:2743995/1‑150 (MQ=255)
                          aaaaaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGa                                                                                                                       <  2:257770/150‑1 (MQ=255)
                            aaaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAAc                                                                                                                     >  1:2784186/1‑150 (MQ=255)
                            aaaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAAc                                                                                                                     >  1:1206583/1‑150 (MQ=255)
                             aaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACa                                                                                                                    >  2:1950794/1‑150 (MQ=255)
                             aaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACa                                                                                                                    <  1:447126/150‑1 (MQ=255)
                                    ggCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatg                                                                                                             >  1:3379014/1‑150 (MQ=255)
                                     gCTTGCAGCCGTCGGAGTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatga                                                                                                            >  2:2272872/1‑150 (MQ=255)
                                      cTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatgat                                                                                                           <  2:2667898/150‑1 (MQ=255)
                                      cTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatgat                                                                                                           <  2:1689987/150‑1 (MQ=255)
                                      cTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGAAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatgat                                                                                                           <  2:1750717/150‑1 (MQ=255)
                                       ttGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatgatg                                                                                                          <  1:3172587/150‑1 (MQ=255)
                                         gCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCg                                                                                                        >  1:2223482/1‑150 (MQ=255)
                                          cAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGt                                                                                                       <  1:1623655/150‑1 (MQ=255)
                                             ccGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtct                                                                                                    <  1:2333549/150‑1 (MQ=255)
                                             ccGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtct                                                                                                    <  1:3444961/150‑1 (MQ=255)
                                             ccGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtct                                                                                                    <  1:2333347/150‑1 (MQ=255)
                                              cGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtctc                                                                                                   >  1:1676202/1‑150 (MQ=255)
                                                    aTTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGATCCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGt                                                                                             <  1:1278528/150‑1 (MQ=255)
                                                     tttGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTc                                                                                            <  1:2619539/150‑1 (MQ=255)
                                                        gATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCt                                                                                         <  2:471733/150‑1 (MQ=255)
                                                          tcatcGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGt                                                                                       <  2:1154054/150‑1 (MQ=255)
                                                           catcGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTc                                                                                      <  2:924781/150‑1 (MQ=255)
                                                           catcGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTCCTCTGGCGTCGCTGTc                                                                                      <  1:1759100/150‑1 (MQ=255)
                                                            atcGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTcg                                                                                     <  2:3249513/150‑1 (MQ=255)
                                                             tcgtcgCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTcgc                                                                                    >  1:701312/1‑150 (MQ=255)
                                                              cgtcgCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTcgcg                                                                                   >  2:159444/1‑150 (MQ=255)
                                                                  gccgcGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGtt                                                                               <  2:947682/150‑1 (MQ=255)
                                                                    cgcgcgTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATCAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGACCAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGa                                                                             >  1:3527605/1‑150 (MQ=255)
                                                                    cgcgcgTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGa                                                                             >  1:3120412/1‑150 (MQ=255)
                                                                      cgcgTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGAcc                                                                           <  2:2924154/150‑1 (MQ=255)
                                                                      cgcgTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGAcc                                                                           >  1:1471026/1‑150 (MQ=255)
                                                                        cgtccgtcGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAg                                                                         <  2:2223482/150‑1 (MQ=255)
                                                                           ccgtcGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACt                                                                      <  2:2676755/150‑1 (MQ=255)
                                                                             gtcGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTaa                                                                    <  1:774204/150‑1 (MQ=255)
                                                                               cGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATCAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTaaaa                                                                  >  2:2556628/1‑150 (MQ=255)
                                                                               cGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTaaaa                                                                  <  1:595552/150‑1 (MQ=255)
                                                                               cGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTaaaa                                                                  >  2:1799659/1‑150 (MQ=255)
                                                                                   gggTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCg                                                              >  2:1355318/1‑150 (MQ=255)
                                                                                   gggTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCg                                                              <  2:2035575/150‑1 (MQ=255)
                                                                                    ggTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGt                                                             <  2:2994516/150‑1 (MQ=255)
                                                                                      tAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTAc                                                           >  2:787614/1‑150 (MQ=255)
                                                                                       aaaaCAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTAcc                                                          >  2:1098424/1‑150 (MQ=255)
                                                                                       aaaaCAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTAcc                                                          >  2:140970/1‑150 (MQ=255)
                                                                                        aaaCAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCg                                                         >  2:2549762/1‑150 (MQ=255)
                                                                                        aaaCAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCg                                                         >  2:2145198/1‑150 (MQ=255)
                                                                                         aacaacATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCgg                                                        >  2:24005/1‑150 (MQ=255)
                                                                                          acaacaTTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCggg                                                       >  2:2944868/1‑150 (MQ=255)
                                                                                          acaacaTTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCggg                                                       <  2:2053006/150‑1 (MQ=255)
                                                                                              caTTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGc                                                   >  2:704564/1‑150 (MQ=255)
                                                                                                tttGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTc                                                 <  1:3260910/150‑1 (MQ=255)
                                                                                                tttGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTc                                                 <  1:3261535/150‑1 (MQ=255)
                                                                                                tttGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTc                                                 <  1:3405950/150‑1 (MQ=255)
                                                                                                tttGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTc                                                 <  1:1509810/150‑1 (MQ=255)
                                                                                                     gATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGa                                            <  2:2768328/150‑1 (MQ=255)
                                                                                                      aTGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGAc                                           >  2:3342859/1‑150 (MQ=255)
                                                                                                       tGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACg                                          >  2:3263187/1‑150 (MQ=255)
                                                                                                        gAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGa                                         >  2:1177027/1‑150 (MQ=255)
                                                                                                         aaCCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGaa                                        >  1:3267320/1‑150 (MQ=255)
                                                                                                         aaCCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGaa                                        >  1:2961918/1‑150 (MQ=255)
                                                                                                          aCCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAg                                       <  1:2905175/150‑1 (MQ=255)
                                                                                                          aCCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAg                                       >  1:3506170/1‑150 (MQ=255)
                                                                                                          aCCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAg                                       >  2:1464492/1‑150 (MQ=255)
                                                                                                          aCCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAg                                       >  2:1705976/1‑150 (MQ=255)
                                                                                                            cTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGAc                                     <  2:522163/150‑1 (MQ=255)
                                                                                                            cTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGAc                                     >  1:1801291/1‑150 (MQ=255)
                                                                                                               gtcgAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTgg                                  >  1:1645973/1‑150 (MQ=255)
                                                                                                                  gAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGgcg                               >  2:1594017/1‑150 (MQ=255)
                                                                                                                    aaaCGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTAGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGgcgcg                             >  1:3631595/1‑150 (MQ=255)
                                                                                                                     aaCGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGgcgcgc                            >  2:1077080/1‑150 (MQ=255)
                                                                                                                        gcggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAtt                         <  2:1039071/150‑1 (MQ=255)
                                                                                                                          ggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTc                       <  2:3050391/150‑1 (MQ=255)
                                                                                                                          ggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTc                       >  2:3559088/1‑150 (MQ=255)
                                                                                                                           gcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTcc                      <  1:941467/150‑1 (MQ=255)
                                                                                                                            tgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCg                     >  2:2031895/2‑150 (MQ=255)
                                                                                                                            cgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCg                     <  1:1950794/150‑1 (MQ=255)
                                                                                                                                gTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCAc                 <  1:24005/150‑1 (MQ=255)
                                                                                                                                  tGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCa               <  1:3022135/150‑1 (MQ=255)
                                                                                                                                      ggATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTACGGCTCCATggg           <  2:2625807/150‑1 (MQ=255)
                                                                                                                                       gATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGt          <  2:3767274/150‑1 (MQ=255)
                                                                                                                                        aTAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTa         >  2:2903779/1‑150 (MQ=255)
                                                                                                                                         tAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAt        <  2:3135020/150‑1 (MQ=255)
                                                                                                                                          aaaCCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAtt       <  2:2304624/150‑1 (MQ=255)
                                                                                                                                           aaCCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAttt      >  1:3151560/1‑150 (MQ=255)
                                                                                                                                            aCCGGTTCTTATCGTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAtttt     <  2:63133/150‑1 (MQ=255)
                                                                                                                                             ccGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTATTTTg    <  2:3383785/150‑1 (MQ=255)
                                                                                                                                              cGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTATTTTGc   >  1:214945/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTACTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTATTTTGCT  >  CP009273/4254863‑4255155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑