Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,266,318 G→T R307L (CGT→CTT)  yjcC → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,266,3180GT100.0% 766.7 / NA 230R307L (CGT→CTT) yjcCputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (108/122);  total (108/122)

Only 100 of 232 total aligned reads displayed.

TATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGAT  >  CP009273/4266171‑4266463
                                                                                                                                                   |                                                                                                                                                 
 aTTACTCTGGCTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTg                                                                                                                                                >  2:3542426/1‑150 (MQ=255)
    aCTCTGGCTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCTATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGcc                                                                                                                                             <  1:3765457/150‑1 (MQ=255)
    aCTCTGGCTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGcc                                                                                                                                             <  2:3446471/150‑1 (MQ=255)
     ctctGGCTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCt                                                                                                                                            <  2:2820489/150‑1 (MQ=255)
        tGGCTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGt                                                                                                                                         >  1:3557877/1‑150 (MQ=255)
         ggCTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTg                                                                                                                                        <  1:943838/150‑1 (MQ=255)
           cTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCTACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAg                                                                                                                                      <  1:1470875/150‑1 (MQ=255)
           cTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAg                                                                                                                                      <  1:1471749/150‑1 (MQ=255)
             aCGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCa                                                                                                                                    <  2:1028988/150‑1 (MQ=255)
                  ttCGACAACACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGc                                                                                                                               <  2:2220465/150‑1 (MQ=255)
                      aCAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAaat                                                                                                                           >  1:728069/1‑150 (MQ=255)
                       cAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAaata                                                                                                                          >  1:2813786/1‑150 (MQ=255)
                          aaCTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATg                                                                                                                       >  2:1273036/1‑150 (MQ=255)
                                   tcacccAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCa                                                                                                              <  1:841242/147‑1 (MQ=255)
                                    cacccAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCag                                                                                                             >  1:3462908/3‑150 (MQ=255)
                                        cAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGtt                                                                                                         >  1:971591/1‑150 (MQ=255)
                                          aaCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTa                                                                                                       >  2:3237164/1‑150 (MQ=255)
                                           aCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTAt                                                                                                      <  2:721608/150‑1 (MQ=255)
                                            cGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTAtt                                                                                                     <  2:2938338/150‑1 (MQ=255)
                                            cGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTAtt                                                                                                     <  2:1401904/150‑1 (MQ=255)
                                              tAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTcc                                                                                                   <  2:751831/150‑1 (MQ=255)
                                              tAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTcc                                                                                                   >  1:1971325/1‑150 (MQ=255)
                                               aaaTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCg                                                                                                  >  1:2241119/1‑150 (MQ=255)
                                               aaaTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCg                                                                                                  >  1:1436961/1‑150 (MQ=255)
                                               aaaTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCg                                                                                                  <  2:2620321/150‑1 (MQ=255)
                                                aaTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGc                                                                                                 >  1:1749865/1‑150 (MQ=255)
                                                aaTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGc                                                                                                 >  1:2882714/1‑150 (MQ=255)
                                                 aTTGCAACGCGCCCTCGAAACACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCt                                                                                                <  2:3057580/150‑1 (MQ=255)
                                                  ttGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTg                                                                                               >  2:629751/1‑150 (MQ=255)
                                                   tGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTgg                                                                                              >  2:3732602/1‑150 (MQ=255)
                                                   tGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTgg                                                                                              <  2:3579817/150‑1 (MQ=255)
                                                     cAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCa                                                                                            >  1:1992056/1‑150 (MQ=255)
                                                      aaCGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAg                                                                                           >  2:150302/1‑150 (MQ=255)
                                                      aaCGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAg                                                                                           >  2:336882/1‑150 (MQ=255)
                                                           gcCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAag                                                                                      >  2:2080284/1‑150 (MQ=255)
                                                             ccTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAagga                                                                                    <  1:188462/150‑1 (MQ=255)
                                                                cGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAggg                                                                                 >  1:2684701/1‑150 (MQ=255)
                                                                cGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAggg                                                                                 >  1:3171526/1‑150 (MQ=255)
                                                                 gAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAgggg                                                                                >  2:3209227/1‑150 (MQ=255)
                                                                  aaaaaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGga                                                                               >  2:2459187/1‑150 (MQ=255)
                                                                   aaaaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgat                                                                              <  1:3405143/150‑1 (MQ=255)
                                                                    aaaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatg                                                                             >  2:538025/1‑150 (MQ=255)
                                                                      aCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatgat                                                                           <  1:1643861/150‑1 (MQ=255)
                                                                       cATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATa                                                                          <  1:2331612/150‑1 (MQ=255)
                                                                       cATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATa                                                                          <  1:1690780/150‑1 (MQ=255)
                                                                            aCTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACa                                                                     <  1:2826286/150‑1 (MQ=255)
                                                                            aCTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACa                                                                     >  1:500294/1‑150 (MQ=255)
                                                                             cTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAg                                                                    <  2:383228/150‑1 (MQ=255)
                                                                              tttGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGa                                                                   <  1:1207689/150‑1 (MQ=255)
                                                                               ttGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGAt                                                                  <  2:1189842/150‑1 (MQ=255)
                                                                                tGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATa                                                                 <  2:426932/150‑1 (MQ=255)
                                                                                  tCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAAc                                                               <  1:121453/150‑1 (MQ=255)
                                                                                   cTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACt                                                              >  2:3055313/1‑150 (MQ=255)
                                                                                    tttATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTg                                                             >  1:3598973/1‑150 (MQ=255)
                                                                                     ttattaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGa                                                            <  1:3503939/150‑1 (MQ=255)
                                                                                      tattaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAt                                                           >  1:3153292/1‑150 (MQ=255)
                                                                                       attaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAtt                                                          >  1:1128694/1‑150 (MQ=255)
                                                                                       attaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAtt                                                          >  2:1211306/1‑150 (MQ=255)
                                                                                         taCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTAt                                                        >  2:773890/1‑150 (MQ=255)
                                                                                         taCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTAt                                                        >  1:2131008/1‑150 (MQ=255)
                                                                                          aCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATg                                                       >  2:1479585/1‑150 (MQ=255)
                                                                                           ccAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGt                                                      <  1:2562688/150‑1 (MQ=255)
                                                                                           ccAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGt                                                      >  2:3012205/1‑150 (MQ=255)
                                                                                           ccAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTCGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGt                                                      <  1:2563043/150‑1 (MQ=255)
                                                                                              gCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTAt                                                   <  1:1617009/150‑1 (MQ=255)
                                                                                              gCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTAt                                                   <  1:2204170/150‑1 (MQ=255)
                                                                                               ccAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTAtt                                                  <  1:3734283/150‑1 (MQ=255)
                                                                                               ccAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTAtt                                                  >  2:1183664/1‑150 (MQ=255)
                                                                                                cAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTg                                                 <  1:376166/150‑1 (MQ=255)
                                                                                                cAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTg                                                 >  2:1667823/1‑150 (MQ=255)
                                                                                                  ataatCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGAt                                               <  1:3237164/150‑1 (MQ=255)
                                                                                                    tatCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATaa                                             >  1:1834256/2‑150 (MQ=255)
                                                                                                     atCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAAt                                            <  1:1274211/150‑1 (MQ=255)
                                                                                                     atCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAAt                                            >  2:1633611/1‑150 (MQ=255)
                                                                                                      tCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATg                                           <  1:162889/150‑1 (MQ=255)
                                                                                                       cGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGt                                          >  2:3802296/1‑150 (MQ=255)
                                                                                                       cGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGt                                          <  2:1749865/150‑1 (MQ=255)
                                                                                                        gATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTc                                         >  2:568807/1‑150 (MQ=255)
                                                                                                         atatCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCt                                        <  1:3683979/150‑1 (MQ=255)
                                                                                                         atatCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCt                                        >  2:2413540/1‑150 (MQ=255)
                                                                                                          tatCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCtt                                       <  2:1870696/150‑1 (MQ=255)
                                                                                                             cAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCcg                                    >  2:3245145/1‑150 (MQ=255)
                                                                                                             cAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCcg                                    <  1:2515518/150‑1 (MQ=255)
                                                                                                                     aaaaaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTgg                            >  2:931305/1‑150 (MQ=255)
                                                                                                                      aaaaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTggg                           <  1:150302/150‑1 (MQ=255)
                                                                                                                       aaaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGc                          >  1:2976399/1‑150 (MQ=255)
                                                                                                                        aaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCg                         <  2:3415467/150‑1 (MQ=255)
                                                                                                                        aaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCg                         <  1:2933/150‑1 (MQ=255)
                                                                                                                        aaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCg                         >  2:2605122/1‑150 (MQ=255)
                                                                                                                         aTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGa                        <  2:727816/150‑1 (MQ=255)
                                                                                                                           gTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGAtt                      >  2:2045578/1‑150 (MQ=255)
                                                                                                                            tATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGTGAAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGTATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTa                     <  2:228504/150‑1 (MQ=255)
                                                                                                                               cGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCt                  <  1:3120619/150‑1 (MQ=255)
                                                                                                                               cGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCt                  <  1:1008604/150‑1 (MQ=255)
                                                                                                                                  cgcTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGc               <  2:2384433/150‑1 (MQ=255)
                                                                                                                                    cTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCaa             >  1:1432813/1‑150 (MQ=255)
                                                                                                                                       aaGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAacac          <  2:684361/150‑1 (MQ=255)
                                                                                                                                         gCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACAt        <  1:2061383/150‑1 (MQ=255)
                                                                                                                                         gCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACAt        <  2:1678523/150‑1 (MQ=255)
                                                                                                                                           gttgttACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGc      >  1:2395140/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGAT  >  CP009273/4266171‑4266463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑