BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,297,485	G→C	G44G (GGC→GGG)	alsK ←	D‑allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,297,485	0	G	C	99.5%	756.0 / NA	192	G44G (GGC→GGG)	alsK	D‑allose kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (86/105); minor base A (1/0); total (87/105)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

CCGCTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTC  >  CP009273/4297340‑4297629

ccgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCgg                                                                                                                                              >  1:3385516/1‑150 (MQ=255)

 cgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCggg                                                                                                                                             <  1:1707479/150‑1 (MQ=255)

   cTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAg                                                                                                                                           <  2:2828608/150‑1 (MQ=255)

   cTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAg                                                                                                                                           <  1:2761207/150‑1 (MQ=255)

    tGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTTAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGc                                                                                                                                          <  2:1437593/150‑1 (MQ=255)

    tGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTTAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGc                                                                                                                                          <  2:3715448/150‑1 (MQ=255)

       tAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAAt                                                                                                                                       <  1:3378535/150‑1 (MQ=255)

          cGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCTGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGAc                                                                                                                                    <  2:3462703/150‑1 (MQ=255)

          cGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGAc                                                                                                                                    <  1:933107/150‑1 (MQ=255)

          cGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGAc                                                                                                                                    >  1:732193/1‑150 (MQ=255)

          cGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGAc                                                                                                                                    >  2:3061033/1‑150 (MQ=255)

          cGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGAc                                                                                                                                    <  1:283404/150‑1 (MQ=255)

          cGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGAc                                                                                                                                    <  1:2301872/150‑1 (MQ=255)

           ggCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACt                                                                                                                                   >  2:969819/1‑150 (MQ=255)

           ggCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACt                                                                                                                                   <  1:2026439/150‑1 (MQ=255)

               ggTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTg                                                                                                                               <  1:3473305/150‑1 (MQ=255)

                  tAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCgg                                                                                                                            >  2:1933001/1‑150 (MQ=255)

                  tAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCgg                                                                                                                            >  2:383050/1‑150 (MQ=255)

                  tAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCgg                                                                                                                            >  1:258731/1‑150 (MQ=255)

                   aGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGt                                                                                                                           <  1:3539163/150‑1 (MQ=255)

                   aGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGt                                                                                                                           >  2:2108069/1‑150 (MQ=255)

                    ggCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTc                                                                                                                          <  2:2727302/150‑1 (MQ=255)

                       gTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGc                                                                                                                       >  1:3697368/1‑150 (MQ=255)

                       gTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGc                                                                                                                       >  2:449130/1‑150 (MQ=255)

                          gAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCttt                                                                                                                    <  2:225171/150‑1 (MQ=255)

                           aaaTAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCtttt                                                                                                                   <  1:481034/150‑1 (MQ=255)

                            aataatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCttttt                                                                                                                  <  2:158803/150‑1 (MQ=255)

                            aataatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCttttt                                                                                                                  <  2:1189285/150‑1 (MQ=255)

                             ataatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCtttttt                                                                                                                 >  2:2665348/1‑150 (MQ=255)

                               aatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTc                                                                                                               <  1:620389/150‑1 (MQ=255)

                                 tGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGc                                                                                                             >  1:2270093/1‑150 (MQ=255)

                                     gCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAgtg                                                                                                         <  1:1123109/150‑1 (MQ=255)

                                      cGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAgtgt                                                                                                        <  1:3551251/150‑1 (MQ=255)

                                       ttttATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTa                                                                                                       >  1:156119/2‑150 (MQ=255)

                                        tttATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAg                                                                                                      <  2:963550/150‑1 (MQ=255)

                                           aTCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGt                                                                                                   >  1:1861910/1‑150 (MQ=255)

                                               ttACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCa                                                                                               >  2:3196764/1‑150 (MQ=255)

                                                tACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCAc                                                                                              >  2:763346/1‑150 (MQ=255)

                                                  cTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCt                                                                                            >  1:1885718/1‑150 (MQ=255)

                                                   tGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCtt                                                                                           <  2:675449/150‑1 (MQ=255)

                                                     aCCAGCGCCGGATATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTct                                                                                         <  2:2351248/150‑1 (MQ=255)

                                                     aCCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTct                                                                                         >  2:910772/1‑150 (MQ=255)

                                                     aCCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTct                                                                                         <  2:3180870/150‑1 (MQ=255)

                                                     aCCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTct                                                                                         <  2:3012801/150‑1 (MQ=255)

                                                      ccAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTctg                                                                                        <  2:1769974/150‑1 (MQ=255)

                                                       cAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTctgc                                                                                       >  1:3068806/1‑150 (MQ=255)

                                                       cAGCGCCGGAAATCCAATCACCAGACCATGAAAGCGAGCGTTAAATAGCCTGAGATGCTCGTCAATCATTTCTCCGATACCCGACACCAACCCGGGAGCAATGACATCTGCGGTCCGCTTTTTATCGCAGTGTAGCGTTTCACCTTcaac                                                                                       >  1:3068197/1‑147 (MQ=255)

                                                        aGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTctgct                                                                                      <  1:1385278/150‑1 (MQ=255)

                                                         gcgcCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTctgctg                                                                                     <  2:2498395/150‑1 (MQ=255)

                                                            cctgAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGTGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTcc                                                                                  <  2:3378023/147‑1 (MQ=255)

                                                            ccGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTcc                                                                                  <  1:1145490/150‑1 (MQ=255)

                                                            ccGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTcc                                                                                  <  1:288568/150‑1 (MQ=255)

                                                             cGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCg                                                                                 >  2:3312955/1‑150 (MQ=255)

                                                              ggAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGc                                                                                >  2:3696709/1‑150 (MQ=255)

                                                              ggAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGc                                                                                >  2:459612/1‑150 (MQ=255)

                                                                     ccATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACaaa                                                                         <  1:1919992/150‑1 (MQ=255)

                                                                       aTCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAg                                                                       >  2:1555685/1‑150 (MQ=255)

                                                                        tCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGc                                                                      <  1:2845761/150‑1 (MQ=255)

                                                                         cACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCACTGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCg                                                                     >  2:1307271/1‑150 (MQ=255)

                                                                          aCCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCgg                                                                    >  1:2316927/1‑150 (MQ=255)

                                                                           ccAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGa                                                                   <  1:3060105/150‑1 (MQ=255)

                                                                            cAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGat                                                                  >  1:2306623/1‑150 (MQ=255)

                                                                                   tGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGtt                                                           >  2:207777/1‑150 (MQ=255)

                                                                                    gACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTg                                                          <  1:1045430/150‑1 (MQ=255)

                                                                                        gcgagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGcccc                                                      <  2:1218264/150‑1 (MQ=255)

                                                                                         cgagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGccccc                                                     <  1:459612/150‑1 (MQ=255)

                                                                                          gagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCa                                                    >  2:1914622/1‑150 (MQ=255)

                                                                                             cgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCatat                                                 >  1:1445226/1‑150 (MQ=255)

                                                                                              gTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATc                                                >  2:624631/1‑150 (MQ=255)

                                                                                                  aaGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACg                                            >  2:1846856/1‑150 (MQ=255)

                                                                                                  aaGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACg                                            <  2:1811821/150‑1 (MQ=255)

                                                                                                  aaGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACg                                            >  2:3643554/1‑150 (MQ=255)

                                                                                                  aaGCGCCTGAGGTGCTCGTCAATCATTTCGCCGAGACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACg                                            <  1:3376349/150‑1 (MQ=255)

                                                                                                     cgcCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGccc                                         <  1:1028080/150‑1 (MQ=255)

                                                                                                     cgcCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGccc                                         <  2:1885718/150‑1 (MQ=255)

                                                                                                      gcCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCg                                        >  1:1978472/1‑150 (MQ=255)

                                                                                                      gcCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCg                                        >  1:68053/1‑150 (MQ=255)

                                                                                                        cTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCt                                      <  1:884061/150‑1 (MQ=255)

                                                                                                         tGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTa                                     >  2:2793181/1‑150 (MQ=255)

                                                                                                         tGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTa                                     >  2:3651117/1‑150 (MQ=255)

                                                                                                             ttGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTacgac                                 >  1:38793/1‑150 (MQ=255)

                                                                                                              tGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTacgacg                                >  1:2971599/1‑150 (MQ=255)

                                                                                                                cTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGtt                              <  1:969819/150‑1 (MQ=255)

                                                                                                                   gTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATg                           >  1:2727199/1‑150 (MQ=255)

                                                                                                                     cAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCt                         <  2:2438979/150‑1 (MQ=255)

                                                                                                                     cAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCt                         <  2:125010/150‑1 (MQ=255)

                                                                                                                       aTCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGt                       <  1:3196764/150‑1 (MQ=255)

                                                                                                                       aTCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGt                       <  1:3730835/150‑1 (MQ=255)

                                                                                                                         cATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGttt                     <  1:2184657/150‑1 (MQ=255)

                                                                                                                          aTTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGtttt                    <  1:2158483/150‑1 (MQ=255)

                                                                                                                             tCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGc                 >  2:1306085/1‑150 (MQ=255)

                                                                                                                               gCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCAt               <  1:2108059/150‑1 (MQ=255)

                                                                                                                                ccGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATg              >  2:2032564/1‑150 (MQ=255)

                                                                                                                                  gATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAg            <  1:3619187/150‑1 (MQ=255)

                                                                                                                                   aTACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAgg           <  2:3507864/150‑1 (MQ=255)

                                                                                                                                    tACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGc          <  1:805113/150‑1 (MQ=255)

                                                                                                                                     aCCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCt         <  1:910772/150‑1 (MQ=255)

                                                                                                                                     aCCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCt         >  1:1513004/1‑150 (MQ=255)

                                                                                                                                        cGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTa      >  1:1894816/1‑150 (MQ=255)

                                                                                                                                          acacCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTAcc    >  2:3398565/1‑150 (MQ=255)

CCGCTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTC  >  CP009273/4297340‑4297629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`