Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,303,614 T→A *150R (TGA→AGA)  rpiB → ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,303,6140TA100.0% 750.6 / NA 224*150R (TGA→AGA) rpiBribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (109/115);  total (109/115)

Only 100 of 225 total aligned reads displayed.

ACACCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATTGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCG  >  CP009273/4303468‑4303760
                                                                                                                                                  |                                                                                                                                                  
 cacCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAgaga                                                                                                                                                <  2:594201/150‑1 (MQ=255)
  acCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGAt                                                                                                                                               <  1:425389/150‑1 (MQ=255)
   ccAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGAtt                                                                                                                                              >  1:1128149/1‑150 (MQ=255)
   ccAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGAtt                                                                                                                                              >  1:986817/1‑150 (MQ=255)
    cAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTc                                                                                                                                             <  1:3294566/150‑1 (MQ=255)
    cAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTc                                                                                                                                             >  2:1395942/1‑150 (MQ=255)
      aCGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCAt                                                                                                                                           <  2:2913073/150‑1 (MQ=255)
        gTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATcc                                                                                                                                         <  1:830704/150‑1 (MQ=255)
        gTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATcc                                                                                                                                         <  1:2988387/150‑1 (MQ=255)
         tGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCa                                                                                                                                        <  2:1584586/150‑1 (MQ=255)
          gCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCac                                                                                                                                       <  1:3367648/150‑1 (MQ=255)
            tGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGAAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCacta                                                                                                                                     <  2:804743/150‑1 (MQ=255)
              gCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCactact                                                                                                                                   <  2:2290879/150‑1 (MQ=255)
               cTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACtt                                                                                                                                  >  2:3349494/1‑150 (MQ=255)
                  ttGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCa                                                                                                                               >  1:3802159/1‑150 (MQ=255)
                  ttGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCa                                                                                                                               >  1:2914301/1‑150 (MQ=255)
                      ttCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGa                                                                                                                           <  1:2032135/150‑1 (MQ=255)
                        cACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATg                                                                                                                         >  1:548873/1‑150 (MQ=255)
                         aCGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGa                                                                                                                        <  1:2547653/150‑1 (MQ=255)
                          cGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAg                                                                                                                       <  1:2725863/150‑1 (MQ=255)
                           gAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGt                                                                                                                      <  2:2806977/150‑1 (MQ=255)
                           gAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGt                                                                                                                      <  2:1816388/150‑1 (MQ=255)
                            aGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTa                                                                                                                     >  1:2641115/1‑150 (MQ=255)
                            aGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTa                                                                                                                     <  1:787660/150‑1 (MQ=255)
                             gTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTaa                                                                                                                    <  2:1269070/150‑1 (MQ=255)
                               ggTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATg                                                                                                                  <  2:1883741/150‑1 (MQ=255)
                                 ttGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGAt                                                                                                                <  2:2428664/150‑1 (MQ=255)
                                    gCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTaa                                                                                                             <  1:1685088/150‑1 (MQ=255)
                                     ccTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAAt                                                                                                            >  1:2131635/1‑150 (MQ=255)
                                      cTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAAtg                                                                                                           >  1:1062727/1‑150 (MQ=255)
                                      cTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAAtg                                                                                                           <  1:615321/150‑1 (MQ=255)
                                      cTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAAtg                                                                                                           <  2:842823/150‑1 (MQ=255)
                                          aaCTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGa                                                                                                       <  1:1429341/150‑1 (MQ=255)
                                            cTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATa                                                                                                     >  2:1614421/1‑150 (MQ=255)
                                             tGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATag                                                                                                    >  2:2556828/1‑150 (MQ=255)
                                              ggCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATaga                                                                                                   >  2:118071/1‑150 (MQ=255)
                                                     aTGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCtt                                                                                            <  1:3579641/150‑1 (MQ=255)
                                                        aTTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCttttt                                                                                         >  2:1844235/1‑150 (MQ=255)
                                                          tgtgGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGa                                                                                       <  2:3549575/150‑1 (MQ=255)
                                                          tgtgGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGa                                                                                       <  2:1064476/150‑1 (MQ=255)
                                                          tgtgGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGa                                                                                       >  1:1500484/1‑150 (MQ=255)
                                                               aTGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTg                                                                                  >  1:2266454/1‑150 (MQ=255)
                                                               aTGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTg                                                                                  >  2:3720006/1‑150 (MQ=255)
                                                                tGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTgg                                                                                 <  1:2730229/150‑1 (MQ=255)
                                                                  cGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCt                                                                               >  1:1510368/1‑150 (MQ=255)
                                                                     ggCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAAt                                                                            <  1:3179754/150‑1 (MQ=255)
                                                                     ggCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAAt                                                                            <  1:575912/150‑1 (MQ=255)
                                                                       cTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGa                                                                          >  2:73442/1‑150 (MQ=255)
                                                                       cTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAAGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGAGGAGTAATGATTAATGTGGATCGAGTTTCGTTTTGAGGTTGGCTCATGa                                                                          >  2:2326471/1‑150 (MQ=255)
                                                                        tGGGCGCACAGTACGAAGGCGGTCGTCATCAACCACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGaa                                                                         <  1:1334325/150‑1 (MQ=255)
                                                                        tGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGaa                                                                         <  1:1937859/150‑1 (MQ=255)
                                                                         gggCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGaaa                                                                        >  2:855635/1‑150 (MQ=255)
                                                                         gggCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGaaa                                                                        <  1:2411302/150‑1 (MQ=255)
                                                                             gcACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCt                                                                    <  1:706928/150‑1 (MQ=255)
                                                                              cacaGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCtt                                                                   <  1:1524638/150‑1 (MQ=255)
                                                                                 aGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTcc                                                                >  2:2614197/1‑150 (MQ=255)
                                                                                 aGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTcc                                                                <  2:963906/150‑1 (MQ=255)
                                                                                 aGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTcc                                                                <  2:1220476/150‑1 (MQ=255)
                                                                                  gTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCg                                                               <  2:3724267/150‑1 (MQ=255)
                                                                                   tACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGc                                                              >  2:1011422/1‑150 (MQ=255)
                                                                                    aCGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCt                                                             <  2:3802159/150‑1 (MQ=255)
                                                                                     cGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTa                                                            <  2:1146674/150‑1 (MQ=255)
                                                                                     cGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTa                                                            >  2:1934265/1‑150 (MQ=255)
                                                                                      gAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAt                                                           >  1:346773/1‑150 (MQ=255)
                                                                                      gAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAt                                                           >  1:3725851/1‑150 (MQ=255)
                                                                                        aGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAttt                                                         >  1:2004851/1‑150 (MQ=255)
                                                                                              tcgtcATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTa                                                   <  2:2622561/150‑1 (MQ=255)
                                                                                              tcgtcATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTa                                                   <  2:480480/150‑1 (MQ=255)
                                                                                                gtcATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTAtt                                                 <  1:1882995/150‑1 (MQ=255)
                                                                                                  catcaACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCa                                               >  1:3699675/1‑150 (MQ=255)
                                                                                                   atcaACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCAc                                              >  2:3454527/1‑150 (MQ=255)
                                                                                                   atcaACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCAc                                              >  1:1618795/1‑150 (MQ=255)
                                                                                                      aacaacGCGTGGCGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCt                                           <  2:3174855/150‑1 (MQ=255)
                                                                                                      aacaacGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCt                                           >  2:28838/1‑150 (MQ=255)
                                                                                                      aacaacGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCt                                           >  2:1329972/1‑150 (MQ=255)
                                                                                                       acaacGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTg                                          <  2:1231606/150‑1 (MQ=255)
                                                                                                         aacGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCt                                        >  1:2384113/1‑150 (MQ=255)
                                                                                                         aacGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCt                                        >  1:739265/1‑150 (MQ=255)
                                                                                                            gcgTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCag                                     <  2:3681237/150‑2 (MQ=255)
                                                                                                             cgTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACg                                    >  2:2268613/1‑150 (MQ=255)
                                                                                                               tGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCt                                  >  1:1229746/1‑150 (MQ=255)
                                                                                                               tGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCt                                  >  1:3624974/1‑150 (MQ=255)
                                                                                                               tGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCt                                  <  2:1543458/150‑1 (MQ=255)
                                                                                                                   ggCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTcc                              <  2:2034001/150‑1 (MQ=255)
                                                                                                                    gCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCa                             <  1:926344/150‑1 (MQ=255)
                                                                                                                    gCGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCa                             <  1:2239650/150‑1 (MQ=255)
                                                                                                                     cGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCAc                            >  1:3035824/1‑150 (MQ=255)
                                                                                                                     cGATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCAc                            >  1:2349135/1‑150 (MQ=255)
                                                                                                                      gATTACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCAcc                           >  1:898457/1‑150 (MQ=255)
                                                                                                                         tACGGCAATAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGtt                        <  1:2614197/150‑1 (MQ=255)
                                                                                                                               aaTAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGcc                  <  2:2914301/150‑1 (MQ=255)
                                                                                                                               aaTAGAGCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGcc                  >  2:893697/1‑150 (MQ=255)
                                                                                                                                  agagCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAg               >  2:1130579/1‑150 (MQ=255)
                                                                                                                                   gagCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAgg              >  1:727732/1‑150 (MQ=255)
                                                                                                                                   gagCAGCGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAgg              <  1:2089077/150‑1 (MQ=255)
                                                                                                                                    agcagcGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGc             >  2:136803/1‑150 (MQ=255)
                                                                                                                                      cagcGGAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCg           <  2:2716096/150‑1 (MQ=255)
                                                                                                                                          ggAGAAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGatat       >  1:1006990/1‑150 (MQ=255)
                                                                                                                                            agaAATAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGAtatta     >  1:1983922/1‑150 (MQ=255)
                                                                                                                                              aaaTAGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATc   >  1:3489663/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACACCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATTGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCG  >  CP009273/4303468‑4303760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑