Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,337,551 C→A V337V (GTG→GTT)  dcuB ← C4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,337,5510CA99.6% 762.8 / NA 231V337V (GTG→GTT) dcuBC4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (128/102);  total (128/103)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

CAAGCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGATTGGTTT  >  CP009273/4337405‑4337696
                                                                                                                                                  |                                                                                                                                                 
cAAGCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAAcc                                                                                                                                                <  2:1599622/150‑1 (MQ=255)
  aNCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCAt                                                                                                                                              >  1:3077554/1‑150 (MQ=255)
  aGCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACACTGGCATAGGCCCACGGATACTCTTTAACCAt                                                                                                                                              >  2:2154273/1‑150 (MQ=255)
  aGCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCAt                                                                                                                                              >  2:3412885/1‑150 (MQ=255)
   gCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCAtt                                                                                                                                             <  1:3267108/150‑1 (MQ=255)
     cGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTc                                                                                                                                           <  2:1899698/150‑1 (MQ=255)
          cTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCa                                                                                                                                      >  2:242357/1‑150 (MQ=255)
           tGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAg                                                                                                                                     >  2:412081/1‑150 (MQ=255)
             aaGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTa                                                                                                                                   >  1:3323780/1‑150 (MQ=255)
             aaGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTa                                                                                                                                   >  2:1857055/1‑150 (MQ=255)
              aGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTAc                                                                                                                                  <  1:732577/150‑1 (MQ=255)
               gCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCTGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACg                                                                                                                                 <  2:2582483/150‑1 (MQ=255)
               gCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCGGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACg                                                                                                                                 >  2:1984238/1‑150 (MQ=255)
                 cACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCCGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGcc                                                                                                                               >  2:475194/1‑150 (MQ=255)
                  aCGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGccc                                                                                                                              >  2:2243629/1‑150 (MQ=255)
                   cGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCt                                                                                                                             <  1:2308844/150‑1 (MQ=255)
                     aTGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGa                                                                                                                           <  2:2748336/150‑1 (MQ=255)
                        tATGCCGGATCTACGCCGATCGACAGCGCGACCGGAACTTTCGCCGCCAGCGGCGCTGCCTGAGAGTTTACAATCTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGaagt                                                                                                                        <  2:2449724/150‑3 (MQ=255)
                         aTGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAAttt                                                                                                                       <  1:2620823/150‑1 (MQ=255)
                            ccGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAg                                                                                                                    <  1:3721080/150‑1 (MQ=255)
                             cGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGa                                                                                                                   >  1:3241910/1‑150 (MQ=255)
                                aTCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACat                                                                                                                <  1:3061677/150‑1 (MQ=255)
                                 tCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACata                                                                                                               >  2:2193968/1‑150 (MQ=255)
                                  cAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGACTTTCAGACatat                                                                                                              >  2:1020317/1‑150 (MQ=255)
                                   aaCGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATg                                                                                                             >  1:2486075/1‑150 (MQ=255)
                                   aaCGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATg                                                                                                             >  1:447640/1‑150 (MQ=255)
                                           tCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGa                                                                                                     >  2:3264331/1‑150 (MQ=255)
                                            cGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGaa                                                                                                    >  1:1910396/1‑150 (MQ=255)
                                            cGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGaa                                                                                                    <  1:2933968/150‑1 (MQ=255)
                                             gCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAAc                                                                                                   <  1:2652857/150‑1 (MQ=255)
                                              ccAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACa                                                                                                  >  1:1008477/1‑150 (MQ=255)
                                              ccAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACa                                                                                                  <  2:1280508/150‑1 (MQ=255)
                                                   gcgACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGtt                                                                                             <  2:2096161/150‑1 (MQ=255)
                                                      aCCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCt                                                                                          <  1:1075444/150‑1 (MQ=255)
                                                       ccGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTg                                                                                         <  2:3196326/150‑1 (MQ=255)
                                                       ccGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTg                                                                                         <  1:1171825/150‑1 (MQ=255)
                                                         ggAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGcc                                                                                       >  1:433431/1‑150 (MQ=255)
                                                              aaTCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGccatcca                                                                                  >  1:2618991/1‑150 (MQ=255)
                                                                tCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATg                                                                                >  1:3200500/1‑150 (MQ=255)
                                                                 cgccgcCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGc                                                                               >  1:451814/1‑150 (MQ=255)
                                                                       cAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATAcc                                                                         >  1:3103983/1‑150 (MQ=255)
                                                                       cAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATAcc                                                                         >  2:1363994/1‑150 (MQ=255)
                                                                        aGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGAAACTCTATAACCATTTCACCCAGTACGCCCTGACTATCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCg                                                                        >  1:3459560/1‑150 (MQ=255)
                                                                           gccgcAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTac                                                                     <  2:3567914/150‑1 (MQ=255)
                                                                            ccgcAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTaca                                                                    >  2:546758/1‑150 (MQ=255)
                                                                             cgcAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTacac                                                                   >  2:168849/1‑150 (MQ=255)
                                                                             cgcAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTacac                                                                   >  1:2114979/1‑150 (MQ=255)
                                                                             cgcAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTacac                                                                   >  1:3448295/1‑150 (MQ=255)
                                                                                  ccTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACACTGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCa                                                              <  2:3486105/150‑1 (MQ=255)
                                                                                  ccTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCa                                                              >  1:2700998/1‑150 (MQ=255)
                                                                                   cTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCAc                                                             <  1:2178346/150‑1 (MQ=255)
                                                                                   cTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCAc                                                             >  2:2903555/1‑150 (MQ=255)
                                                                                      agagTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGAt                                                          >  2:274015/1‑150 (MQ=255)
                                                                                      agagTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGAt                                                          <  1:200303/150‑1 (MQ=255)
                                                                                       gagTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATg                                                         <  1:3406437/150‑1 (MQ=255)
                                                                                          tttACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCg                                                      >  1:484255/1‑150 (MQ=255)
                                                                                             aCAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATc                                                   >  2:3004976/1‑150 (MQ=255)
                                                                                             aCAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATc                                                   >  1:3776952/1‑150 (MQ=255)
                                                                                               aaaCTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCAt                                                 >  1:1109219/1‑150 (MQ=255)
                                                                                                aaCTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATa                                                >  2:1729529/1‑150 (MQ=255)
                                                                                                  cTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATAcc                                              <  2:2619492/150‑1 (MQ=255)
                                                                                                   ttGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACcg                                             <  1:2243629/150‑1 (MQ=255)
                                                                                                     ggAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACcgga                                           >  2:317311/1‑150 (MQ=255)
                                                                                                        aaCCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACcggaacg                                        >  1:3734819/1‑150 (MQ=255)
                                                                                                            agcagcagAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAg                                    <  1:1936069/150‑1 (MQ=255)
                                                                                                            agcagcagAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAg                                    >  2:2470771/1‑150 (MQ=255)
                                                                                                             gcagcagAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGa                                   <  1:1706537/150‑1 (MQ=255)
                                                                                                              cagcagAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGAc                                  >  2:2809879/1‑150 (MQ=255)
                                                                                                               agcagAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACt                                 >  1:1246362/1‑150 (MQ=255)
                                                                                                               agcagAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACt                                 >  2:2881031/1‑150 (MQ=255)
                                                                                                               agcagAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACt                                 >  1:1284979/1‑150 (MQ=255)
                                                                                                                 cagAACATTGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTc                               <  1:2154273/150‑1 (MQ=255)
                                                                                                                 cagAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTc                               <  1:3412885/150‑1 (MQ=255)
                                                                                                                  agAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCg                              >  2:333479/1‑150 (MQ=255)
                                                                                                                   gAACAATGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGt                             <  2:435912/150‑1 (MQ=255)
                                                                                                                       aaTGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAGTTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGttttt                         <  2:110878/150‑1 (MQ=255)
                                                                                                                       aaTGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGttttt                         >  1:3343183/1‑150 (MQ=255)
                                                                                                                         tGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTg                       >  1:3482806/1‑150 (MQ=255)
                                                                                                                         tGGCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTg                       >  2:343359/1‑150 (MQ=255)
                                                                                                                           gCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTgag                     <  1:1101130/150‑1 (MQ=255)
                                                                                                                           gCATAGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTgag                     <  2:130964/150‑1 (MQ=255)
                                                                                                                               aGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAg                 >  2:3286029/1‑150 (MQ=255)
                                                                                                                               aGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAg                 >  1:679299/1‑150 (MQ=255)
                                                                                                                               aGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAg                 >  1:787814/1‑150 (MQ=255)
                                                                                                                               aGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAg                 <  2:114747/150‑1 (MQ=255)
                                                                                                                               aGGCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAg                 >  2:1388069/1‑150 (MQ=255)
                                                                                                                                ggCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGa                >  1:2610881/1‑150 (MQ=255)
                                                                                                                                ggCCCACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGa                <  2:373720/150‑1 (MQ=255)
                                                                                                                                  cccACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGAcg              >  2:2336529/1‑150 (MQ=255)
                                                                                                                                   ccACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGAcgc             <  1:1363994/150‑1 (MQ=255)
                                                                                                                                    cACGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGAcgcg            >  1:3026167/1‑150 (MQ=255)
                                                                                                                                      cGGATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCggg          >  1:2590395/1‑150 (MQ=255)
                                                                                                                                       ggATACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGa         <  1:2323293/150‑1 (MQ=255)
                                                                                                                                         aTACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGAtt       <  2:1910396/150‑1 (MQ=255)
                                                                                                                                         aTACTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGAtt       >  2:448349/1‑150 (MQ=255)
                                                                                                                                           aCTCTTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGATTgg     <  1:3570442/150‑1 (MQ=255)
                                                                                                                                            ctctTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGATTGGt    <  2:1948919/150‑1 (MQ=255)
                                                                                                                                            ctctTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGATTGGt    <  2:250218/150‑1 (MQ=255)
                                                                                                                                             tctTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGATTGGtt   >  1:1415990/1‑150 (MQ=255)
                                                                                                                                              ctTTAACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGATTGGttt  <  2:730191/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CAAGCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCGACCGGAACAATCGCCGCCAGCGCCGCAGCCTGAGAGTTTACAAACTTGGAAACCAGCAGCAGAACAATGGCATAGGCCCACGGATACTCTTTCACCATTTCACCCAGTACGCCCTGAATTTCAGACATATGCGCACCGAACATGGTTTCTGCCATCCATGCGATACCGTACACCGCCACGATGGCGATCATACCGGAACGGAAGACTTCGTTTTTTGAGATAGACGCGGGATTGGTTT  >  CP009273/4337405‑4337696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑