BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,341,023	A→T	Y153N (TAC→AAC)	dcuS ←	sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,341,023	0	A	T	100.0%	669.4 / NA	201	Y153N (TAC→AAC)	dcuS	sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (103/98); total (103/98)

Only 100 of 201 total aligned reads displayed.

CAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTT  >  CP009273/4340877‑4341169

cAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGAt                                                                                                                                                 >  1:140104/1‑150 (MQ=255)

cAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGAt                                                                                                                                                 >  2:3451995/1‑150 (MQ=255)

 aGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATg                                                                                                                                                >  1:1120051/1‑150 (MQ=255)

 aGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATg                                                                                                                                                >  1:886577/1‑150 (MQ=255)

 aGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATg                                                                                                                                                >  1:233908/1‑150 (MQ=255)

  gAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATgg                                                                                                                                               <  1:1797237/150‑1 (MQ=255)

   aaTGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATggg                                                                                                                                              >  2:3751162/1‑150 (MQ=255)

    aTGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATgggg                                                                                                                                             >  2:145627/1‑150 (MQ=255)

     tGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATggggg                                                                                                                                            >  2:1242370/1‑150 (MQ=255)

          gTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAAt                                                                                                                                       <  1:3401329/150‑1 (MQ=255)

            gCCAATCAGTCCGACCAGCATGCCAAATTATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATAc                                                                                                                                     <  2:590815/150‑1 (MQ=255)

            gCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATAc                                                                                                                                     >  1:1228667/1‑150 (MQ=255)

            gCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATAc                                                                                                                                     >  1:1229129/1‑150 (MQ=255)

                 tCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTa                                                                                                                                <  1:1171647/150‑1 (MQ=255)

                   aGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTaaa                                                                                                                              <  2:2349612/150‑1 (MQ=255)

                     tCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGc                                                                                                                            >  2:659014/1‑150 (MQ=255)

                      ccGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGcc                                                                                                                           >  2:3638810/1‑150 (MQ=255)

                        gACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTg                                                                                                                         >  1:769896/1‑150 (MQ=255)

                        gACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTg                                                                                                                         <  1:1435811/150‑1 (MQ=255)

                                tGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGaa                                                                                                                 >  2:2167544/1‑150 (MQ=255)

                                  ccAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGaaaa                                                                                                               >  2:487725/1‑150 (MQ=255)

                                   cAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAAc                                                                                                              <  2:2942639/150‑1 (MQ=255)

                                      ataataCCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACcgc                                                                                                           >  1:3575873/1‑150 (MQ=255)

                                       taataCCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACcgcg                                                                                                          <  1:157070/150‑1 (MQ=255)

                                          taCCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGAtt                                                                                                       >  1:2062896/1‑150 (MQ=255)

                                              gACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATa                                                                                                   >  2:175707/1‑150 (MQ=255)

                                               aCCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAg                                                                                                  >  1:2863684/1‑150 (MQ=255)

                                                ccAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGc                                                                                                 <  2:1375578/150‑1 (MQ=255)

                                                 cAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCg                                                                                                >  2:2027914/1‑150 (MQ=255)

                                                   gATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGAc                                                                                              <  2:606746/150‑1 (MQ=255)

                                                             cAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCg                                                                                    <  2:51534/150‑1 (MQ=255)

                                                             cAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCg                                                                                    <  1:3484360/150‑1 (MQ=255)

                                                                 gACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCAt                                                                                >  2:1803812/1‑150 (MQ=255)

                                                                 gACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCAt                                                                                <  1:673668/150‑1 (MQ=255)

                                                                 gACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCAt                                                                                >  1:2705301/1‑150 (MQ=255)

                                                                    tGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCa                                                                             >  2:376678/1‑150 (MQ=255)

                                                                     gTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAg                                                                            >  1:393102/1‑150 (MQ=255)

                                                                     gTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAg                                                                            <  2:2684269/150‑1 (MQ=255)

                                                                     gTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAg                                                                            <  2:3039107/150‑1 (MQ=255)

                                                                      tCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAgc                                                                           >  1:2355915/1‑150 (MQ=255)

                                                                      tCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAgc                                                                           <  2:1048220/150‑1 (MQ=255)

                                                                         ttGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCt                                                                        <  1:2490049/150‑1 (MQ=255)

                                                                          tGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCtt                                                                       >  2:2009046/1‑150 (MQ=255)

                                                                          tGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCtt                                                                       >  2:3341216/1‑150 (MQ=255)

                                                                             tCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTaa                                                                    <  2:1218988/150‑1 (MQ=255)

                                                                              cTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAg                                                                   <  1:147152/150‑1 (MQ=255)

                                                                              cTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAg                                                                   >  1:832848/1‑150 (MQ=255)

                                                                               tGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAgg                                                                  <  2:2873869/150‑1 (MQ=255)

                                                                                gTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGa                                                                 <  2:2459627/150‑1 (MQ=255)

                                                                                gTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGa                                                                 <  2:324272/150‑1 (MQ=255)

                                                                                 ttGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGAt                                                                >  1:2799735/1‑150 (MQ=255)

                                                                                 ttGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGAt                                                                <  2:2473766/150‑1 (MQ=255)

                                                                                  tGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATg                                                               <  2:2062876/150‑1 (MQ=255)

                                                                                    ggTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGtc                                                             >  1:741567/1‑150 (MQ=255)

                                                                                       cacaCGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGtcatc                                                          <  2:443089/150‑1 (MQ=255)

                                                                                         cacGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCAc                                                        >  1:642466/1‑150 (MQ=255)

                                                                                              cTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTa                                                   >  2:2815059/1‑150 (MQ=255)

                                                                                              cTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTa                                                   <  1:2676883/150‑1 (MQ=255)

                                                                                               ttAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTaa                                                  >  2:3379372/1‑150 (MQ=255)

                                                                                                tAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTaaa                                                 >  2:2138488/1‑150 (MQ=255)

                                                                                                   cTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATgg                                              <  1:3452735/150‑1 (MQ=255)

                                                                                                   cTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATgg                                              <  2:2605444/150‑1 (MQ=255)

                                                                                                   cTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATgg                                              <  2:2075541/150‑1 (MQ=255)

                                                                                                        ggCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGTTTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGAc                                         <  2:2742966/150‑1 (MQ=255)

                                                                                                         gCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGAcc                                        <  1:1878602/150‑1 (MQ=255)

                                                                                                          ccGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCa                                       <  2:1291249/150‑1 (MQ=255)

                                                                                                           cGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCaa                                      <  1:2027914/150‑1 (MQ=255)

                                                                                                            gATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAGAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAAt                                     <  2:1424617/150‑1 (MQ=255)

                                                                                                            gATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAAt                                     <  2:848287/150‑1 (MQ=255)

                                                                                                             aTCGCCACCACGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATa                                    >  2:863257/1‑150 (MQ=255)

                                                                                                                 ccaccaCGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCt                                >  2:1444531/1‑150 (MQ=255)

                                                                                                                 ccaccaCGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCt                                <  2:3476494/150‑1 (MQ=255)

                                                                                                                   accacGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTgg                              >  1:1715760/1‑150 (MQ=255)

                                                                                                                   accacGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTgg                              >  2:1269067/1‑150 (MQ=255)

                                                                                                                    ccacGCCAAGTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTggg                             <  1:2787372/150‑1 (MQ=255)

                                                                                                                     cacGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGc                            >  1:3016812/1‑150 (MQ=255)

                                                                                                                     cacGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGc                            >  1:3015598/1‑150 (MQ=255)

                                                                                                                     cacGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGc                            >  2:3607585/1‑150 (MQ=255)

                                                                                                                       cGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCtt                          <  2:3247403/150‑1 (MQ=255)

                                                                                                                       cGCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCtt                          <  2:769896/150‑1 (MQ=255)

                                                                                                                        gCCAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTc                         >  2:183851/1‑150 (MQ=255)

                                                                                                                         ccAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCa                        >  1:1937994/1‑150 (MQ=255)

                                                                                                                          cAATTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAg                       >  2:1537854/1‑150 (MQ=255)

                                                                                                                           aaTTTGTTTATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAgg                      >  2:3439194/1‑150 (MQ=255)

                                                                                                                                 tttATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGa                >  2:2237579/1‑150 (MQ=255)

                                                                                                                                  ttATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAg               <  1:1602608/150‑1 (MQ=255)

                                                                                                                                   tATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGt              <  1:487725/150‑1 (MQ=255)

                                                                                                                                   tATGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGt              <  2:309975/150‑1 (MQ=255)

                                                                                                                                    aTGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTa             >  1:2580815/1‑150 (MQ=255)

                                                                                                                                     tGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAg            <  1:2612743/150‑1 (MQ=255)

                                                                                                                                     tGATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAg            <  1:175707/150‑1 (MQ=255)

                                                                                                                                      gATTTTCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGc           <  2:676038/150‑1 (MQ=255)

                                                                                                                                          ttCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAg       <  2:1882242/150‑1 (MQ=255)

                                                                                                                                           tCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGa      >  2:1640674/1‑150 (MQ=255)

                                                                                                                                           tCATCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGa      <  1:3530153/150‑1 (MQ=255)

                                                                                                                                             aTCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACt    >  2:2927720/1‑150 (MQ=255)

                                                                                                                                             aTCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACt    <  1:2446546/150‑1 (MQ=255)

                                                                                                                                             aTCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACt    >  2:1144918/1‑150 (MQ=255)

                                                                                                                                             aTCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACt    <  2:2327821/150‑1 (MQ=255)

                                                                                                                                              tCGTTGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACtt   <  1:343699/150‑1 (MQ=255)

CAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATACGCGTAAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTT  >  CP009273/4340877‑4341169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`