Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,350,223 G→A A510V (GCT→GTT)  cadC ← cadBA operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,350,2230GA99.6% 790.7 / NA 237A510V (GCT→GTT) cadCcadBA operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (125/111);  minor base C (1/0);  total (126/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

GATTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATT  >  CP009273/4350076‑4350369
                                                                                                                                                   |                                                                                                                                                  
gATTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGACCATACAAAAACTCAACAACAAATATTTCCGAGCCTAAATCAACCCGGAGTTACTTATTCTGAAACa                                                                                                                                                  >  1:3313667/1‑150 (MQ=255)
gATTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACa                                                                                                                                                  >  1:168892/1‑150 (MQ=255)
   tGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGa                                                                                                                                               <  1:2720320/150‑1 (MQ=255)
     cGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGaaa                                                                                                                                             >  1:1406486/1‑150 (MQ=255)
     cGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGaaa                                                                                                                                             >  2:1081757/1‑150 (MQ=255)
       tGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAtt                                                                                                                                           <  1:1425820/150‑1 (MQ=255)
        gACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAttt                                                                                                                                          >  2:3553093/1‑150 (MQ=255)
        gACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAttt                                                                                                                                          >  2:3645333/1‑150 (MQ=255)
        gACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAttt                                                                                                                                          >  2:3645594/1‑150 (MQ=255)
          cATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGt                                                                                                                                        <  2:1135053/150‑1 (MQ=255)
           aTTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTc                                                                                                                                       <  1:1417327/150‑1 (MQ=255)
              ctctGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCgag                                                                                                                                    >  1:2839498/1‑150 (MQ=255)
               tctGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCgaga                                                                                                                                   <  1:2490190/150‑1 (MQ=255)
                 tGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATa                                                                                                                                 <  2:2077065/150‑1 (MQ=255)
                  ggTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATaa                                                                                                                                <  2:3393918/150‑1 (MQ=255)
                   gTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAg                                                                                                                               >  1:3636818/1‑150 (MQ=255)
                       aaTTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATCTGTCGAGATAAGGTac                                                                                                                           >  2:2800632/1‑150 (MQ=255)
                        aTTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTaca                                                                                                                          >  1:2332614/1‑150 (MQ=255)
                          ttATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTacaac                                                                                                                        >  2:2843111/1‑150 (MQ=255)
                          ttATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTacaac                                                                                                                        >  1:1181629/1‑150 (MQ=255)
                            aTGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACAt                                                                                                                      <  1:3469160/150‑1 (MQ=255)
                                     aaaTTATGCGTCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAg                                                                                                             <  2:3620459/150‑1 (MQ=255)
                                     aaaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAg                                                                                                             >  1:589824/1‑150 (MQ=255)
                                     aaaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAg                                                                                                             <  2:1053773/150‑1 (MQ=255)
                                        ttATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAg                                                                                                          >  2:1027969/1‑150 (MQ=255)
                                         tATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGt                                                                                                         <  2:2714937/150‑1 (MQ=255)
                                         tATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGt                                                                                                         >  2:1792211/1‑150 (MQ=255)
                                         tATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTCATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGt                                                                                                         <  1:1241425/150‑1 (MQ=255)
                                          aTGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTc                                                                                                        <  1:3763821/150‑1 (MQ=255)
                                            gCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTg                                                                                                      >  2:1972580/1‑150 (MQ=255)
                                             cGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTgg                                                                                                     <  2:373747/150‑1 (MQ=255)
                                              ggCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGa                                                                                                    <  1:3119212/150‑1 (MQ=255)
                                                cAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAat                                                                                                  <  1:255753/150‑1 (MQ=255)
                                                cAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAat                                                                                                  >  2:682653/1‑150 (MQ=255)
                                                cAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAat                                                                                                  >  1:1030561/1‑150 (MQ=255)
                                                cAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAat                                                                                                  <  2:1952251/150‑1 (MQ=255)
                                                 aaataaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAata                                                                                                 >  1:3594781/1‑150 (MQ=255)
                                                  aataaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAatat                                                                                                >  1:3738095/1‑150 (MQ=255)
                                                  aataaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAatat                                                                                                >  1:2177569/1‑150 (MQ=255)
                                                   ataaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAAtata                                                                                               >  2:3767799/1‑150 (MQ=255)
                                                   ataaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAAtata                                                                                               >  2:1553575/1‑150 (MQ=255)
                                                     aaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATAcc                                                                                             >  2:933895/1‑150 (MQ=255)
                                                     aaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATAcc                                                                                             >  1:2279639/1‑150 (MQ=255)
                                                       atTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACGAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAt                                                                                           >  2:701558/1‑150 (MQ=255)
                                                       atTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAt                                                                                           >  1:2802340/1‑150 (MQ=255)
                                                        ttGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAtt                                                                                          <  2:1259594/150‑1 (MQ=255)
                                                         tGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAttt                                                                                         >  2:1169969/1‑150 (MQ=255)
                                                          gCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAtttt                                                                                        <  1:362963/150‑1 (MQ=255)
                                                           ccGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTc                                                                                       <  1:2255722/150‑1 (MQ=255)
                                                              cAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAAt                                                                                    <  2:1575511/150‑1 (MQ=255)
                                                                aCATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATcc                                                                                  <  2:3543460/150‑1 (MQ=255)
                                                                    attatACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTa                                                                              >  2:309087/1‑150 (MQ=255)
                                                                         aCCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAggg                                                                         <  2:1840209/150‑1 (MQ=255)
                                                                          ccAACAGGAACATACAAAAACTCAACAACTAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGgt                                                                        <  1:701558/150‑1 (MQ=255)
                                                                          ccAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGgt                                                                        >  1:1879269/1‑150 (MQ=255)
                                                                            aaCAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGgtgt                                                                      >  2:1610074/1‑150 (MQ=255)
                                                                              cAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGttt                                                                    <  2:1406486/150‑1 (MQ=255)
                                                                               aGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTg                                                                   >  2:2537902/1‑150 (MQ=255)
                                                                                 gAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGcc                                                                 <  1:1962022/150‑1 (MQ=255)
                                                                                   aCATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGcccc                                                               >  2:3266500/1‑150 (MQ=255)
                                                                                    cATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCt                                                              <  2:1199266/150‑1 (MQ=255)
                                                                                           aaaCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTa                                                       <  2:1258939/150‑1 (MQ=255)
                                                                                           aaaCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTa                                                       <  1:1913741/150‑1 (MQ=255)
                                                                                            aaCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTaa                                                      <  2:2415306/150‑1 (MQ=255)
                                                                                             aCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTaaa                                                     <  1:2024714/150‑1 (MQ=255)
                                                                                             aCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTaaa                                                     <  2:918097/150‑1 (MQ=255)
                                                                                              cTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAAt                                                    >  2:60063/1‑150 (MQ=255)
                                                                                                caacaacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTa                                                  <  1:3010431/150‑1 (MQ=255)
                                                                                                  acaacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTaaa                                                >  1:3257642/1‑150 (MQ=255)
                                                                                                    aacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAgg                                              <  2:2927526/150‑1 (MQ=255)
                                                                                                     acaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGc                                             <  1:933895/150‑1 (MQ=255)
                                                                                                       aaaTATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCgg                                           >  1:522821/1‑150 (MQ=255)
                                                                                                       aaaTATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCgg                                           >  1:3232248/1‑150 (MQ=255)
                                                                                                         atatTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTg                                         <  2:2822034/150‑1 (MQ=255)
                                                                                                          tatTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTga                                        <  1:2202159/150‑1 (MQ=255)
                                                                                                          tatTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTga                                        <  1:3344886/150‑1 (MQ=255)
                                                                                                            tttCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTgaga                                      <  2:2458866/150‑1 (MQ=255)
                                                                                                            tntCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTgaga                                      >  1:2442177/1‑150 (MQ=255)
                                                                                                               ccGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGatat                                   >  1:60809/1‑150 (MQ=255)
                                                                                                                     aTAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCa                             >  2:1884100/1‑150 (MQ=255)
                                                                                                                     aTAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCa                             >  2:3165467/1‑150 (MQ=255)
                                                                                                                     aTAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCa                             >  2:3198426/1‑150 (MQ=255)
                                                                                                                      tAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAg                            <  1:3595879/150‑1 (MQ=255)
                                                                                                                      tAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAg                            >  1:1110817/1‑150 (MQ=255)
                                                                                                                       aaaTCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAgc                           >  2:504207/1‑150 (MQ=255)
                                                                                                                            aaCCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCtt                      <  1:527904/150‑1 (MQ=255)
                                                                                                                              cccGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTcc                    <  1:3645520/150‑1 (MQ=255)
                                                                                                                              cccGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTcc                    >  1:739100/1‑150 (MQ=255)
                                                                                                                               ccGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGAAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTccc                   >  2:3340656/1‑150 (MQ=255)
                                                                                                                                    gTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTc              >  2:3691315/1‑150 (MQ=255)
                                                                                                                                     ttACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCa             >  1:1219707/1‑150 (MQ=255)
                                                                                                                                      tACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCAt            >  2:2153350/1‑150 (MQ=255)
                                                                                                                                        cTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTGCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATcc          >  2:810944/1‑150 (MQ=255)
                                                                                                                                         ttATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATccc         <  1:3767799/150‑1 (MQ=255)
                                                                                                                                         ttATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATccc         <  1:292081/150‑1 (MQ=255)
                                                                                                                                         ttATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATccc         >  1:1723014/1‑150 (MQ=255)
                                                                                                                                            ttCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCtt      >  1:2534424/1‑150 (MQ=255)
                                                                                                                                            ttCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTAAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCtt      <  1:3529762/150‑1 (MQ=255)
                                                                                                                                              cTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCttca    >  1:1048468/1‑150 (MQ=255)
                                                                                                                                                gAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGACCAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCttcatt  <  2:2279639/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATT  >  CP009273/4350076‑4350369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑