BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,352,529	C→A	intergenic (‑86/+21)	pheU ← / ← yjdC	tRNA‑Phe/putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,352,529	0	C	A	100.0%	719.6 / NA	216	intergenic (‑86/+21)	pheU/yjdC	tRNA‑Phe/putative transcriptional regulator
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (108/108); total (108/108)

Only 100 of 216 total aligned reads displayed.

GGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTAAACAGCCTTCCAGCAC  >  CP009273/4352382‑4352675

ggAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGc                                                                                                                                                  >  1:2113279/1‑150 (MQ=255)

 gAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGct                                                                                                                                                 <  1:2709571/150‑1 (MQ=255)

 gAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGct                                                                                                                                                 <  1:400100/150‑1 (MQ=255)

 gAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGct                                                                                                                                                 <  2:3572167/150‑1 (MQ=255)

   aTCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGcttc                                                                                                                                               >  1:3454624/1‑150 (MQ=255)

         ccAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCt                                                                                                                                         <  2:2257907/150‑1 (MQ=255)

         ccAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCt                                                                                                                                         <  2:3680787/150‑1 (MQ=255)

          cAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCtg                                                                                                                                        <  1:573421/150‑1 (MQ=255)

           aaGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCtgt                                                                                                                                       <  1:1697422/150‑1 (MQ=255)

            aGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTGAGATTGCGGCAAATAGCTTCTTTTCtgtg                                                                                                                                      >  2:2023497/1‑150 (MQ=255)

            aGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCtgtg                                                                                                                                      >  1:807462/1‑150 (MQ=255)

            aGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCtgtg                                                                                                                                      >  2:2716340/1‑150 (MQ=255)

            aGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCtgtg                                                                                                                                      >  2:1984140/1‑150 (MQ=255)

             ggACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTgtgt                                                                                                                                     <  1:3315334/150‑1 (MQ=255)

              gACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGtt                                                                                                                                    <  2:3801890/150‑1 (MQ=255)

              gACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGtt                                                                                                                                    <  1:954628/150‑1 (MQ=255)

               acacGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGttt                                                                                                                                   <  1:2411152/150‑1 (MQ=255)

               acacGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGttt                                                                                                                                   <  1:2412268/150‑1 (MQ=255)

                 acGGGGATTTTCACTCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCt                                                                                                                                 <  2:3729930/150‑1 (MQ=255)

                 acGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCt                                                                                                                                 <  2:1835248/150‑1 (MQ=255)

                   ggggATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGt                                                                                                                               <  2:3436567/150‑1 (MQ=255)

                         tttCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTc                                                                                                                         <  2:490909/150‑1 (MQ=255)

                            cAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAAt                                                                                                                      <  2:1326785/150‑1 (MQ=255)

                            cAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAAt                                                                                                                      >  2:2246511/1‑150 (MQ=255)

                             aaTCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATg                                                                                                                     >  2:3547385/1‑150 (MQ=255)

                                ccccTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGcac                                                                                                                  >  1:2526873/1‑150 (MQ=255)

                                     gCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCAccc                                                                                                             <  1:2547972/150‑1 (MQ=255)

                                      ctctACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCt                                                                                                            <  2:496763/150‑1 (MQ=255)

                                       tctACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTg                                                                                                           <  1:2665109/150‑1 (MQ=255)

                                         tACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTggc                                                                                                         >  2:1954734/1‑150 (MQ=255)

                                             gACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCa                                                                                                     >  2:1137233/1‑150 (MQ=255)

                                              aCTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAg                                                                                                    <  2:434395/150‑1 (MQ=255)

                                                tGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCg                                                                                                  >  1:1376001/1‑150 (MQ=255)

                                                 gAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCgg                                                                                                 >  1:2694370/1‑150 (MQ=255)

                                                     tATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGa                                                                                             <  1:3545167/150‑1 (MQ=255)

                                                      aTCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGaa                                                                                            <  2:2894126/150‑1 (MQ=255)

                                                      aTCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGaa                                                                                            >  2:2624041/1‑150 (MQ=255)

                                                       tCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAg                                                                                           <  2:1260561/150‑1 (MQ=255)

                                                           ggCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCa                                                                                       >  2:171185/1‑150 (MQ=255)

                                                              aaCGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGa                                                                                    >  2:3111734/1‑150 (MQ=255)

                                                              aaCGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGa                                                                                    <  2:1586630/150‑1 (MQ=255)

                                                              aaCGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGa                                                                                    >  1:2771145/1‑150 (MQ=255)

                                                                 ggggCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGTGAAGCGCAGGatat                                                                                 <  1:1842558/150‑1 (MQ=255)

                                                                 ggggCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGatat                                                                                 >  2:223663/1‑150 (MQ=255)

                                                                 ggggCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGatat                                                                                 >  1:3404766/1‑150 (MQ=255)

                                                                      gcATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTcc                                                                            >  1:1888600/1‑150 (MQ=255)

                                                                      gcATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTcc                                                                            <  2:2877544/150‑1 (MQ=255)

                                                                       cATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCg                                                                           <  1:1603597/150‑1 (MQ=255)

                                                                             aCCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGcc                                                                     >  1:1682196/1‑150 (MQ=255)

                                                                                gTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGAt                                                                  >  2:3401254/1‑150 (MQ=255)

                                                                                 tAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGAtg                                                                 >  1:1131224/1‑150 (MQ=255)

                                                                                   aTCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGAtgtg                                                               >  1:1068290/1‑150 (MQ=255)

                                                                                   aTCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGAtgtg                                                               <  1:365042/150‑1 (MQ=255)

                                                                                   aTCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGAtgtg                                                               <  2:1858480/150‑1 (MQ=255)

                                                                                     cTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCa                                                             >  2:2801026/1‑150 (MQ=255)

                                                                                     cTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCa                                                             <  1:37545/150‑1 (MQ=255)

                                                                                        cacaTCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAgtg                                                          <  2:3454862/150‑1 (MQ=255)

                                                                                          caTCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTc                                                        <  2:2526873/150‑1 (MQ=255)

                                                                                            tctcGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGa                                                      >  1:3101474/1‑150 (MQ=255)

                                                                                             ctcGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGAc                                                     >  2:530394/1‑150 (MQ=255)

                                                                                             ctcGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGAc                                                     >  1:2249024/1‑150 (MQ=255)

                                                                                                 tCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATcc                                                 <  1:2895994/150‑1 (MQ=255)

                                                                                                  cAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCg                                                >  1:1330849/1‑150 (MQ=255)

                                                                                                    aCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGcc                                              <  1:3407960/150‑1 (MQ=255)

                                                                                                     ccTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCt                                             <  1:1871346/150‑1 (MQ=255)

                                                                                                      cTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTg                                            <  2:87686/150‑1 (MQ=255)

                                                                                                      cTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTg                                            >  1:734292/1‑150 (MQ=255)

                                                                                                        aaTTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGc                                          >  2:3091403/1‑150 (MQ=255)

                                                                                                        aaTTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGc                                          >  2:1193452/1‑150 (MQ=255)

                                                                                                        aaTTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGc                                          >  1:2544555/1‑150 (MQ=255)

                                                                                                         aTTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCt                                         >  2:105252/1‑150 (MQ=255)

                                                                                                         aTTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCt                                         >  2:1725414/1‑150 (MQ=255)

                                                                                                             cAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGa                                     >  1:1784439/1‑150 (MQ=255)

                                                                                                               gTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGAtt                                   <  1:1196297/150‑1 (MQ=255)

                                                                                                                  aaaGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCAc                                >  1:1208418/1‑150 (MQ=255)

                                                                                                                   aaGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCAcc                               >  2:641666/1‑150 (MQ=255)

                                                                                                                   aaGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCAcc                               <  2:1788244/150‑1 (MQ=255)

                                                                                                                    aGCGATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCa                              <  1:510920/150‑1 (MQ=255)

                                                                                                                       gATTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCa                           >  1:3143303/1‑150 (MQ=255)

                                                                                                                        aTTCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCAt                          >  2:1850440/1‑150 (MQ=255)

                                                                                                                         ttCAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCAtt                         <  2:3327364/150‑1 (MQ=255)

                                                                                                                           cAACTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCg                       >  1:2325550/1‑150 (MQ=255)

                                                                                                                            aaCTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCgg                      >  2:912295/1‑150 (MQ=255)

                                                                                                                             aCTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGc                     >  2:3255938/1‑150 (MQ=255)

                                                                                                                              cTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCt                    >  1:2180766/1‑150 (MQ=255)

                                                                                                                              cTGCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCt                    >  1:1720968/1‑150 (MQ=255)

                                                                                                                                gCTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTa                  <  2:807462/150‑1 (MQ=255)

                                                                                                                                 cTTAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTaa                 >  1:2499962/1‑150 (MQ=255)

                                                                                                                                   tAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTAAAc               <  2:303820/150‑1 (MQ=255)

                                                                                                                                   tAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTAAAc               <  1:1170541/150‑1 (MQ=255)

                                                                                                                                   tAAGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTAAAc               >  1:3269065/1‑150 (MQ=255)

                                                                                                                                    aaGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTAAACa              <  1:2527426/150‑1 (MQ=255)

                                                                                                                                     aGATTGCGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTAAACAg             >  2:2946643/1‑150 (MQ=255)

                                                                                                                                           cGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTAAACAGCCTTcc       <  2:1682196/150‑1 (MQ=255)

                                                                                                                                           cGGCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTAAACAGCCTTca       <  1:3111734/150‑2 (MQ=255)

                                                                                                                                            ggCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTAAACAGCCTTCCa      >  1:462419/1‑150 (MQ=255)

                                                                                                                                             gCAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTAAACAGCCTTCCAg     <  1:361913/150‑1 (MQ=255)

                                                                                                                                              cAAATAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTAAACAGCCTTCCAGc    >  1:3071158/1‑150 (MQ=255)

                                                                                                                                               aaaTAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTAAACAGCCTTCCAGCa   >  2:1554537/1‑150 (MQ=255)

                                                                                                                                                aaTAGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTAAACAGCCTTCCAGCAc  >  1:226765/1‑150 (MQ=255)

GGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGACATCCGCCTGGCTACGATTCACCAGCATTCGGCTTAAACAGCCTTCCAGCAC  >  CP009273/4352382‑4352675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`