Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,358,920 G→T R147L (CGC→CTC)  fxsA → suppressor of F exclusion of phage T7

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,358,9200GT100.0% 772.9 / NA 230R147L (CGC→CTC) fxsAsuppressor of F exclusion of phage T7
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (90/140);  total (90/140)

Only 100 of 238 total aligned reads displayed.

TACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCGCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCG  >  CP009273/4358773‑4359066
                                                                                                                                                   |                                                                                                                                                  
tACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGctcg                                                                                                                                                  >  2:528850/1‑150 (MQ=255)
tACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGctcg                                                                                                                                                  <  2:3674640/150‑1 (MQ=255)
tACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGctcg                                                                                                                                                  <  2:2786714/150‑1 (MQ=255)
tACTTTTAGTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGctcg                                                                                                                                                  <  2:2786404/150‑1 (MQ=255)
   ttttATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGAcc                                                                                                                                               <  1:3461288/150‑1 (MQ=255)
      tATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGcc                                                                                                                                            <  2:3622935/150‑1 (MQ=255)
       aTTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCt                                                                                                                                           >  1:3267425/1‑150 (MQ=255)
       aTTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCt                                                                                                                                           <  1:3208171/150‑1 (MQ=255)
        ttGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCtt                                                                                                                                          <  1:763966/150‑1 (MQ=255)
         tGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTg                                                                                                                                         <  1:1069174/150‑1 (MQ=255)
              ccgGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCAt                                                                                                                                    <  2:1224147/150‑1 (MQ=255)
              ccgGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCAt                                                                                                                                    >  2:1819149/1‑150 (MQ=255)
              ccgGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCAt                                                                                                                                    <  2:1764592/150‑1 (MQ=255)
              ccgGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCAt                                                                                                                                    <  1:327626/150‑1 (MQ=255)
               cgGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATa                                                                                                                                   <  1:2492268/150‑1 (MQ=255)
                    cAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGAc                                                                                                                              <  1:3108792/150‑1 (MQ=255)
                      aaaGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGa                                                                                                                            >  1:3138292/1‑150 (MQ=255)
                       aaGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGAt                                                                                                                           >  2:2861546/1‑150 (MQ=255)
                        aGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATc                                                                                                                          >  2:1004669/1‑150 (MQ=255)
                        aGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATc                                                                                                                          <  2:600963/150‑1 (MQ=255)
                         gCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCg                                                                                                                         <  2:3764724/150‑1 (MQ=255)
                         gCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCg                                                                                                                         >  2:394692/1‑150 (MQ=255)
                         gCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCg                                                                                                                         <  2:565991/150‑1 (MQ=255)
                         gCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCg                                                                                                                         <  2:2031682/150‑1 (MQ=255)
                          cATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGc                                                                                                                        >  2:2404450/1‑150 (MQ=255)
                           aTCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGcc                                                                                                                       <  1:2181453/150‑1 (MQ=255)
                            tCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCa                                                                                                                      <  1:3339470/150‑1 (MQ=255)
                             cTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAg                                                                                                                     >  2:943903/1‑150 (MQ=255)
                               gACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGa                                                                                                                   >  1:2544727/1‑150 (MQ=255)
                                  aGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTa                                                                                                                >  2:2888176/1‑150 (MQ=255)
                                  aGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTa                                                                                                                <  2:1217046/150‑1 (MQ=255)
                                     gAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATg                                                                                                             >  1:3501002/1‑150 (MQ=255)
                                     gAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATg                                                                                                             <  2:3707756/150‑1 (MQ=255)
                                      aaGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGt                                                                                                            <  1:2710807/150‑1 (MQ=255)
                                               ccGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGACACGcc                                                                                                   >  2:947941/1‑150 (MQ=255)
                                                cGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCg                                                                                                  <  2:1639338/150‑1 (MQ=255)
                                                 gCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCgg                                                                                                 <  2:908489/150‑1 (MQ=255)
                                                    tttGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATAATAAAGACGATCGCCAGGCTTAATGTCGAAACGCCGGAtt                                                                                              >  2:3610153/1‑150 (MQ=255)
                                                         gcTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTAtgtg                                                                                         <  1:3670195/150‑1 (MQ=255)
                                                          cTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTgg                                                                                        <  2:234524/150‑1 (MQ=255)
                                                          cTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTgg                                                                                        <  2:3545811/150‑1 (MQ=255)
                                                            tttCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGtt                                                                                      <  2:2671752/150‑1 (MQ=255)
                                                              tctcGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTAt                                                                                    <  1:1731483/150‑1 (MQ=255)
                                                               ctcGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATg                                                                                   <  2:3442207/150‑1 (MQ=255)
                                                                  gCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCa                                                                                <  1:1647457/150‑1 (MQ=255)
                                                                   cATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCAt                                                                               >  2:2266951/1‑150 (MQ=255)
                                                                    aTGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCAtt                                                                              <  1:3782346/150‑1 (MQ=255)
                                                                    aTGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCAtt                                                                              >  2:1940428/1‑150 (MQ=255)
                                                                      gCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCAtttt                                                                            <  1:719774/150‑1 (MQ=255)
                                                                       ccTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTc                                                                           >  2:3200468/1‑150 (MQ=255)
                                                                        cTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTcc                                                                          <  2:2694224/150‑1 (MQ=255)
                                                                           gcggcgGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGc                                                                       <  2:295748/150‑1 (MQ=255)
                                                                           gcggcgGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGc                                                                       <  1:281003/150‑1 (MQ=255)
                                                                           gcggcgGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGc                                                                       <  1:3394803/150‑1 (MQ=255)
                                                                                ggTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGtttt                                                                  <  2:1258050/150‑1 (MQ=255)
                                                                                ggTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGtttt                                                                  <  2:3156640/150‑1 (MQ=255)
                                                                                ggTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGtttt                                                                  <  2:3520475/150‑1 (MQ=255)
                                                                                ggTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGtttt                                                                  >  1:579958/1‑150 (MQ=255)
                                                                                  ttttAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTc                                                                <  2:3454898/150‑1 (MQ=255)
                                                                                   tttAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCg                                                               <  2:656719/150‑1 (MQ=255)
                                                                                   tttAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCg                                                               >  2:2740010/1‑150 (MQ=255)
                                                                                     tAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGtt                                                             >  2:1421234/1‑150 (MQ=255)
                                                                                      aGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGttt                                                            <  1:3438851/150‑1 (MQ=255)
                                                                                       gcgcCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGtttt                                                           <  2:3671540/150‑1 (MQ=255)
                                                                                        cgcCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTg                                                          <  1:1469538/150‑1 (MQ=255)
                                                                                        cgcCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTg                                                          >  2:2335103/1‑150 (MQ=255)
                                                                                            gggACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGgcag                                                      >  1:216047/1‑150 (MQ=255)
                                                                                               aCCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCaa                                                   <  1:2267298/150‑1 (MQ=255)
                                                                                                   gTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGc                                               <  1:2335109/150‑1 (MQ=255)
                                                                                                   gTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGc                                               <  1:2732946/150‑1 (MQ=255)
                                                                                                   gTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGc                                               <  2:865162/150‑1 (MQ=255)
                                                                                                   gTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGc                                               >  1:678676/1‑150 (MQ=255)
                                                                                                     ggCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCa                                             <  2:2496427/150‑1 (MQ=255)
                                                                                                     ggCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCa                                             <  2:3458806/150‑1 (MQ=255)
                                                                                                     ggCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCa                                             >  1:1237335/1‑150 (MQ=255)
                                                                                                      gCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCAt                                            <  1:1006975/150‑1 (MQ=255)
                                                                                                      gCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCAt                                            <  2:2990599/150‑1 (MQ=255)
                                                                                                      gCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCAt                                            <  1:2829070/150‑1 (MQ=255)
                                                                                                        ggTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATaa                                          >  2:1105924/1‑150 (MQ=255)
                                                                                                        ggTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATaa                                          >  2:281026/1‑150 (MQ=255)
                                                                                                            aTACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAAcccc                                      <  1:1337431/150‑1 (MQ=255)
                                                                                                             tACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCg                                     >  1:1962520/1‑150 (MQ=255)
                                                                                                               cTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGcc                                   >  1:2776576/1‑150 (MQ=255)
                                                                                                                    gATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCAt                              >  1:2469405/1‑150 (MQ=255)
                                                                                                                       ggTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGCTCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGAt                           <  2:3363763/150‑1 (MQ=255)
                                                                                                                         tGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCa                         <  2:1691897/150‑1 (MQ=255)
                                                                                                                         tGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCa                         >  1:2030656/1‑150 (MQ=255)
                                                                                                                            gTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAgcg                      <  2:1328015/150‑1 (MQ=255)
                                                                                                                               ccAGCGATTGTATGATGAGCTCGACAGCCTTTATCATATAGTCGATCGAGAGGATTATTGTCGTTACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGTCAGCAACAGCCATAACCCCGCAAGCATGATCAGCGCAt                   <  1:3610153/150‑1 (MQ=255)
                                                                                                                                 aGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATag                 >  1:142311/1‑150 (MQ=255)
                                                                                                                                   cGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATagag               >  2:1177606/1‑150 (MQ=255)
                                                                                                                                    gAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAgaga              >  1:529058/1‑150 (MQ=255)
                                                                                                                                     aaaGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGAc             <  1:1217755/150‑1 (MQ=255)
                                                                                                                                        ggATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACttt          <  1:943903/150‑1 (MQ=255)
                                                                                                                                         gatgatGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACtttt         <  2:2067675/150‑1 (MQ=255)
                                                                                                                                          atgatgAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTc        >  1:619232/1‑150 (MQ=255)
                                                                                                                                           tgatgaGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTcc       >  1:474342/1‑150 (MQ=255)
                                                                                                                                            gatgaGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCa      <  1:1148048/150‑1 (MQ=255)
                                                                                                                                             atgaGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAg     >  2:489433/1‑150 (MQ=255)
                                                                                                                                               gaGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGcc   >  1:457118/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCGCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCG  >  CP009273/4358773‑4359066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑