BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,378,778	G→T	S137* (TCG→TAG)	mscM ←	mechanosensitive channel protein, miniconductance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,378,778	0	G	T	100.0%	831.5 / NA	249	S137* (TCG→TAG)	mscM	mechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (114/135); total (114/135)

Only 100 of 249 total aligned reads displayed.

AGCGCCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTCGTCACGCATGTT  >  CP009273/4378631‑4378923

aGCGCCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTaa                                                                                                                                                 <  1:3668422/150‑1 (MQ=255)

   gcCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCTGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCg                                                                                                                                              <  2:3132894/150‑1 (MQ=255)

   gcCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCg                                                                                                                                              <  1:2946884/150‑1 (MQ=255)

   gcCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCg                                                                                                                                              <  1:2187858/150‑1 (MQ=255)

         aGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAAtc                                                                                                                                        >  1:3575775/1‑150 (MQ=255)

         aGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAAtc                                                                                                                                        >  1:135363/1‑150 (MQ=255)

            cGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTcg                                                                                                                                     <  1:3102814/150‑1 (MQ=255)

            cGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTcg                                                                                                                                     >  1:2380115/1‑150 (MQ=255)

             gTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTcgc                                                                                                                                    <  2:106505/150‑1 (MQ=255)

             gTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTcgc                                                                                                                                    <  2:1805607/150‑1 (MQ=255)

                 agagTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGgc                                                                                                                                >  2:3254425/1‑150 (MQ=255)

                  gagTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGgcg                                                                                                                               <  1:3077523/150‑1 (MQ=255)

                      cAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCt                                                                                                                           <  2:1083685/150‑1 (MQ=255)

                         aCTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCt                                                                                                                        >  1:1416767/1‑150 (MQ=255)

                             cAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGctc                                                                                                                    >  1:1043933/1‑150 (MQ=255)

                             cAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGctc                                                                                                                    >  1:1980828/1‑150 (MQ=255)

                              aaCGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGctct                                                                                                                   <  2:81186/150‑1 (MQ=255)

                              aaCGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGctct                                                                                                                   >  2:453348/1‑150 (MQ=255)

                              aaCGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGAGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGctct                                                                                                                   <  1:2464035/150‑1 (MQ=255)

                               aCGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCtt                                                                                                                  <  2:2875705/150‑1 (MQ=255)

                               aCGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCtt                                                                                                                  <  2:3472382/150‑1 (MQ=255)

                                cgcgAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTg                                                                                                                 <  1:1158971/150‑1 (MQ=255)

                                cgcgAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTg                                                                                                                 <  1:1478969/150‑1 (MQ=255)

                                  cgAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCt                                                                                                               >  1:400428/1‑150 (MQ=255)

                                  cgAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCt                                                                                                               <  1:889329/150‑1 (MQ=255)

                                     aaTTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTggg                                                                                                            >  1:514902/1‑150 (MQ=255)

                                      aTTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGc                                                                                                           <  2:1397610/150‑1 (MQ=255)

                                        tttGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCt                                                                                                         <  2:3785291/150‑1 (MQ=255)

                                         ttGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTg                                                                                                        <  2:2742606/150‑1 (MQ=255)

                                         ttGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTg                                                                                                        <  2:24400/150‑1 (MQ=255)

                                          tgtgCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGa                                                                                                       >  1:2594994/1‑150 (MQ=255)

                                          tgtgCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGa                                                                                                       <  1:3177292/150‑1 (MQ=255)

                                          tgtgCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGa                                                                                                       <  2:2378848/150‑1 (MQ=255)

                                           gtgCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGAc                                                                                                      <  2:1707012/150‑1 (MQ=255)

                                           gtgCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGAc                                                                                                      <  1:1155521/150‑1 (MQ=255)

                                            tgCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACg                                                                                                     <  2:844440/150‑1 (MQ=255)

                                              ccTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGc                                                                                                   <  2:942958/150‑1 (MQ=255)

                                                tGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCtt                                                                                                 >  2:3553571/1‑150 (MQ=255)

                                                tGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCtt                                                                                                 <  1:1296319/150‑1 (MQ=255)

                                                 gATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCttt                                                                                                >  2:1894403/1‑150 (MQ=255)

                                                    tGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTAt                                                                                             <  1:574316/150‑1 (MQ=255)

                                                    tGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTAt                                                                                             >  2:3675277/1‑150 (MQ=255)

                                                       gCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCa                                                                                          >  1:3542726/1‑150 (MQ=255)

                                                         ggAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGc                                                                                        >  1:2849101/1‑150 (MQ=255)

                                                           tgTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCaa                                                                                      <  1:986679/149‑1 (MQ=255)

                                                           aGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCaa                                                                                      <  2:2658484/150‑1 (MQ=255)

                                                            gTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAAc                                                                                     <  2:63927/150‑1 (MQ=255)

                                                             tATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACt                                                                                    <  2:3197918/150‑1 (MQ=255)

                                                              aTTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTg                                                                                   >  1:2459316/1‑150 (MQ=255)

                                                               ttGCCGGTAAGCGTTCCCAGGCGGCACTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTgg                                                                                  >  1:2217627/1‑150 (MQ=255)

                                                                tGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGgc                                                                                 >  1:3663784/1‑150 (MQ=255)

                                                                tGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGgc                                                                                 <  1:1645669/150‑1 (MQ=255)

                                                                  ccGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGgctg                                                                               <  1:2161682/150‑1 (MQ=255)

                                                                  ccGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGgctg                                                                               <  2:2075151/150‑1 (MQ=255)

                                                                  ccGGTAAGCGTTCCCAGGCGGCACTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGgctg                                                                               <  2:2217627/150‑1 (MQ=255)

                                                                     gTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGctgctg                                                                            >  2:1298326/1‑150 (MQ=255)

                                                                       aaGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGAc                                                                          >  1:1835121/1‑150 (MQ=255)

                                                                       aaGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGAc                                                                          <  2:2327708/150‑1 (MQ=255)

                                                                        aGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGAcc                                                                         >  2:3033023/1‑150 (MQ=255)

                                                                           gTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTgg                                                                      >  1:1989641/1‑150 (MQ=255)

                                                                           gTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTgg                                                                      >  1:3323779/1‑150 (MQ=255)

                                                                           gTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTgg                                                                      >  2:3586126/1‑150 (MQ=255)

                                                                           gTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTgg                                                                      >  1:778485/1‑150 (MQ=255)

                                                                           gTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTgg                                                                      <  1:647620/150‑1 (MQ=255)

                                                                            ttCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGga                                                                     >  1:2604642/1‑150 (MQ=255)

                                                                            ttCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGga                                                                     >  2:2174582/1‑150 (MQ=255)

                                                                                 aGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAAtt                                                                >  2:1792470/1‑150 (MQ=255)

                                                                                 aGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTCTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAAtt                                                                <  2:1314855/150‑1 (MQ=255)

                                                                                  ggcggcGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAAttt                                                               <  2:1436050/150‑1 (MQ=255)

                                                                                  ggcggcGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAAttt                                                               <  2:1436086/150‑1 (MQ=255)

                                                                                      gcgcTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTg                                                           >  2:2230853/1‑150 (MQ=255)

                                                                                         cTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGAtt                                                        <  2:2543868/150‑1 (MQ=255)

                                                                                           cGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCa                                                      <  2:446771/150‑1 (MQ=255)

                                                                                           cGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCa                                                      <  2:1126947/150‑1 (MQ=255)

                                                                                             aTCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAgc                                                    >  2:3155743/1‑150 (MQ=255)

                                                                                              tctcATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAgcg                                                   >  1:698035/1‑150 (MQ=255)

                                                                                                  aTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTc                                               <  1:2788918/150‑1 (MQ=255)

                                                                                                     tAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGACTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGt                                            <  2:3413457/150‑1 (MQ=255)

                                                                                                       aCTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAg                                          >  1:1572272/1‑150 (MQ=255)

                                                                                                        cTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGa                                         <  1:3775310/150‑1 (MQ=255)

                                                                                                        cTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGa                                         >  1:849270/1‑150 (MQ=255)

                                                                                                        cTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGa                                         >  2:1960512/1‑150 (MQ=255)

                                                                                                          gACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACa                                       >  1:2293735/1‑150 (MQ=255)

                                                                                                           aCGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACAt                                      <  1:1717638/150‑1 (MQ=255)

                                                                                                           aCGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACAt                                      <  2:168313/150‑1 (MQ=255)

                                                                                                            cggcggGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACAtt                                     >  1:24037/1‑150 (MQ=255)

                                                                                                                 ggcgTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCgg                                >  2:212539/1‑150 (MQ=255)

                                                                                                                   cgTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCg                              <  1:1329140/150‑1 (MQ=255)

                                                                                                                         tttGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGc                        >  2:362448/1‑150 (MQ=255)

                                                                                                                         tttGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGc                        >  2:362888/1‑150 (MQ=255)

                                                                                                                               gTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGcgcg                  >  1:1800619/1‑150 (MQ=255)

                                                                                                                                  gCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCt               <  2:3542726/150‑1 (MQ=255)

                                                                                                                                   cGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTc              <  2:1178482/150‑1 (MQ=255)

                                                                                                                                      cAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTCGTc           >  2:1380838/1‑150 (MQ=255)

                                                                                                                                      cAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTCGTc           <  1:3505857/150‑1 (MQ=255)

                                                                                                                                        gTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTCGTCAc         >  1:3427175/1‑150 (MQ=255)

                                                                                                                                         ttGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTCGTCACg        >  2:3767365/1‑150 (MQ=255)

                                                                                                                                           gATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTCGTCACGCa      >  2:3230640/1‑150 (MQ=255)

                                                                                                                                            aTTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTCGTCACGCAt     <  1:2997157/150‑1 (MQ=255)

                                                                                                                                               cAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTCGTCACGCAtgtt  <  2:1210949/150‑1 (MQ=255)

AGCGCCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTCGTCACGCATGTT  >  CP009273/4378631‑4378923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`