Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,382,218 C→A noncoding (42/76 nt) glyV → tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,382,2180CA93.8% 557.6 / 20.3 209noncoding (42/76 nt)glyVtRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (7/6);  new base A (85/111);  total (92/117)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

AAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCA  >  CP009273/4382071‑4382364
                                                                                                                                                   |                                                                                                                                                  
aaaaaaaaCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAgg                                                                                                                                                  >  2:715374/1‑150 (MQ=255)
  aaaaatCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAgggg                                                                                                                                                >  2:525198/1‑150 (MQ=255)
  aaaaaaCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGTGGGACTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAgggg                                                                                                                                                >  1:2324312/1‑150 (MQ=255)
  aaaaaaCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAgggg                                                                                                                                                >  2:2425545/1‑150 (MQ=255)
  aaaaaaCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAgggg                                                                                                                                                >  2:173833/1‑150 (MQ=255)
   aaaaaCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGt                                                                                                                                               >  2:871279/1‑150 (MQ=255)
   aaaaaCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGt                                                                                                                                               >  1:879866/1‑150 (MQ=255)
    aaaaCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTc                                                                                                                                              >  1:2760250/1‑150 (MQ=255)
     aaaCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTcg                                                                                                                                             >  2:2855638/1‑150 (MQ=255)
     aaaCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTcg                                                                                                                                             <  2:718337/150‑1 (MQ=255)
       aCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTcgcg                                                                                                                                           >  2:3758200/1‑150 (MQ=255)
       aCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTcgcg                                                                                                                                           <  1:3072524/150‑1 (MQ=255)
       aCTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTcgcg                                                                                                                                           <  1:1583332/150‑1 (MQ=255)
            tttGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTc                                                                                                                                      <  1:3758200/150‑1 (MQ=255)
              tGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                                                                                                                                    >  1:601749/1‑150 (MQ=255)
              tGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGa                                                                                                                                    >  1:1847091/1‑150 (MQ=255)
               ggggggTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAg                                                                                                                                   >  1:708685/1‑150 (MQ=255)
               ggggggTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAg                                                                                                                                   >  1:2000330/1‑150 (MQ=255)
               cgggggTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAg                                                                                                                                   >  2:2451856/2‑150 (MQ=255)
                   ggTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGtctc                                                                                                                               <  2:2893884/150‑1 (MQ=255)
                    gTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCg                                                                                                                              >  1:3490073/1‑150 (MQ=255)
                       gCAGCGGGATAGATTTCTCGTATCTTGCGCCTCCCGTAACGACGCATAAATGCGAAAATTACGACAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGttt                                                                                                                           <  2:2106145/150‑1 (MQ=25)
                        cAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                                                                          <  1:3159523/150‑1 (MQ=255)
                         agagGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTcc                                                                                                                         >  1:524713/1‑150 (MQ=255)
                          gagGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTccc                                                                                                                        <  1:3626645/150‑1 (MQ=255)
                          gagGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTccc                                                                                                                        <  1:1084704/150‑1 (MQ=255)
                           agGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGAAGGGGTCGCGAGTTCGAGTCTCGTTTCCCg                                                                                                                       >  1:1863474/1‑150 (MQ=255)
                              gAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTc                                                                                                                    >  2:756833/1‑150 (MQ=255)
                               aatgATTTGTCGTATATTGCGCCTCCCGTTACGACGCAGAAATGCGAAAATTTCGCAACCTCAATGAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTcc                                                                                                                   <  2:601749/147‑1 (MQ=18)
                               aaaGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTcc                                                                                                                   <  1:1566055/150‑1 (MQ=255)
                                            ataATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAg                                                                                                      <  1:3592607/150‑1 (MQ=255)
                                             taATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGt                                                                                                     >  1:3455218/1‑150 (MQ=255)
                                             taATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGt                                                                                                     <  2:2483051/150‑1 (MQ=255)
                                               aTGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGtgc                                                                                                   <  1:428636/150‑1 (MQ=255)
                                                tGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAGATTTGaaagttcat                                                                                                  >  2:730072/1‑144 (MQ=255)
                                                tGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGtgct                                                                                                  >  2:2580544/1‑150 (MQ=255)
                                                tGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGtgct                                                                                                  <  2:2625626/150‑1 (MQ=255)
                                                tGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGtgct                                                                                                  <  1:1689243/150‑1 (MQ=255)
                                                tGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGtgct                                                                                                  <  1:1739723/150‑1 (MQ=255)
                                                     cTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCaaa                                                                                             >  1:3253221/1‑150 (MQ=255)
                                                      tCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAg                                                                                            <  2:2142926/150‑1 (MQ=255)
                                                           tAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGcaaagcaaag                                                                                       >  2:3535376/1‑150 (MQ=255)
                                                            aaCGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGa                                                                                      >  2:236961/1‑150 (MQ=255)
                                                            aaCGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGa                                                                                      <  2:702890/150‑1 (MQ=255)
                                                               gacgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGacca                                                                                   >  1:1511320/1‑150 (MQ=255)
                                                                acgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGaccac                                                                                  <  2:3064609/150‑1 (MQ=255)
                                                                  gCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCAccc                                                                                <  2:2848514/150‑1 (MQ=255)
                                                                    aGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCaa                                                                              >  1:2380916/1‑150 (MQ=255)
                                                                    aGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCaa                                                                              >  2:3090002/1‑150 (MQ=255)
                                                                    aGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCaa                                                                              >  2:2191830/1‑150 (MQ=255)
                                                                     gAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAg                                                                             <  2:1895077/150‑1 (MQ=255)
                                                                     gAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAg                                                                             <  2:3659791/150‑1 (MQ=255)
                                                                      aaaTGCGAAAATTACGAAAGCAAAATTAAGGAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGc                                                                            <  1:439264/150‑1 (MQ=255)
                                                                       aaTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCg                                                                           >  2:817792/1‑150 (MQ=255)
                                                                        aTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCgg                                                                          <  2:3582271/150‑1 (MQ=255)
                                                                          gCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGa                                                                        <  1:2779968/150‑1 (MQ=255)
                                                                           cGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAATGTGGTTAAATGCCAATACCACCCAAGGGGGaa                                                                       >  2:3205778/1‑150 (MQ=17)
                                                                           cGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGaa                                                                       <  2:3267996/150‑1 (MQ=255)
                                                                            gAAAATTACGAAAGCAAACTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                                                                                                                                    <  2:1171822/88‑1 (MQ=255)
                                                                            gAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                                                                                                                                    >  1:1171822/1‑88 (MQ=255)
                                                                            gAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATTAAAACTAATACATATCACAAACCACCCAATCGGGAAt                                                                      >  1:1409412/1‑150 (MQ=18)
                                                                            gAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAAt                                                                      <  2:1009607/150‑1 (MQ=255)
                                                                            gAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAAt                                                                      <  2:3334631/150‑1 (MQ=255)
                                                                               aaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGc                                                                   >  1:2223409/1‑150 (MQ=255)
                                                                                aTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAATTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCt                                                                  >  2:2824024/1‑150 (MQ=255)
                                                                                    cGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGATGCAAAGCACAGACCACCCAAGCGGGAATAGGTCAGt                                                              >  2:1247889/1‑150 (MQ=255)
                                                                                     gAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATGAAAAGTGCTGCAAAGCACATACCACCCAAGCGGGACAAGCTCAGtt                                                             >  1:281909/1‑150 (MQ=17)
                                                                                      aaaGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTg                                                            <  2:1567887/150‑1 (MQ=255)
                                                                                       aaGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTgg                                                           >  2:2499078/1‑150 (MQ=255)
                                                                                          cAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTag                                                        >  1:2189522/1‑150 (MQ=255)
                                                                                           aaaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATGAAAAGTAATACAAAGCACAAACCACCCAAGCGAGAATATCTCAAATGGtcta                                                       >  1:3735847/1‑147 (MQ=17)
                                                                                            aaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGtt                                                                                                                            <  2:1196187/80‑1 (MQ=255)
                                                                                            aaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGtt                                                                                                                            >  1:1196187/1‑80 (MQ=255)
                                                                                            aaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATGAAAAGTGCTGCAAACCACATACCACCCAAGCGGGAAAAGCTAAGTTGGTatag                                                      >  1:1196360/1‑150 (MQ=17)
                                                                                                tAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGATGCAAAGCACAGACCACCCAAGCTGGAATAGCTCAGTAGGTAGAGCACg                                                  >  1:2581047/1‑150 (MQ=255)
                                                                                                  agtagtACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGtt                                                                                                                            >  2:522266/1‑74 (MQ=255)
                                                                                                  agtagtACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGAc                                                <  2:2760250/150‑1 (MQ=255)
                                                                                                    tagtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCt                                              <  2:393930/150‑1 (MQ=255)
                                                                                                     agtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCtt                                             <  1:2688152/150‑1 (MQ=255)
                                                                                                      gtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCCCCCAAGCGGAAAAAGAAAAGTTCGTAGAGCCCGACATTg                                            >  2:1530362/1‑150 (MQ=14)
                                                                                                        aCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGnc                                          <  1:817792/150‑1 (MQ=255)
                                                                                                               aaTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGATGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg                                   >  2:1085079/1‑150 (MQ=255)
                                                                                                                aTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                  <  1:2855638/150‑1 (MQ=255)
                                                                                                                 tAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCggg                                 >  1:2052935/1‑150 (MQ=255)
                                                                                                                      cAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgc                            <  2:2324312/150‑1 (MQ=255)
                                                                                                                      cAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgc                            <  2:1295166/150‑1 (MQ=255)
                                                                                                                        gTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGa                          <  2:343981/150‑1 (MQ=255)
                                                                                                                         ttGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAg                         <  1:163331/150‑1 (MQ=255)
                                                                                                                                agCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGt                  <  2:1847469/150‑1 (MQ=255)
                                                                                                                                  cACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct                <  2:1511320/150‑1 (MQ=255)
                                                                                                                                   ccgcccTTGCGAAGGTAGGGGTCGCGAGTTCGTCTCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTGCAACGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtctc               <  2:2385492/146‑1 (MQ=11)
                                                                                                                                    cGACCTGGCCCAGGTAGGGGTCGCTTGTTCGAGTCACGTTTCCCTCTCCCAAATGTGAAAAGTGCTTCACCTCACAGACCTCCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCg              <  2:879219/150‑1 (MQ=11)
                                                                                                                                      aCCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGtt            <  1:1901134/150‑1 (MQ=255)
                                                                                                                                      aCCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGtt            <  2:1441091/150‑1 (MQ=255)
                                                                                                                                       ccTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGttt           <  2:1642697/150‑1 (MQ=255)
                                                                                                                                        cTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc          <  1:1904333/150‑1 (MQ=255)
                                                                                                                                           gCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAAGTGATACAAATCACAAACCACCCAAGCGGGAATAGCTCAGTTAGTAGAGCACAACCAACCCAAGAACGGGATCGCGAATACGAGTCACGTTTCCCg       >  1:1467705/1‑150 (MQ=14)
                                                                                                                                           gCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGAAGAGCACGACCTTGCCAAGGTCGGGTTCGCGAGTTCGAGTCTCGTTTCCCg       >  1:3679800/1‑150 (MQ=255)
                                                                                                                                               aGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTcc   >  1:3353427/1‑150 (MQ=255)
                                                                                                                                                ggTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCa  <  1:1529482/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCA  >  CP009273/4382071‑4382364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑