Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,394,875 G→T A124A (GCG→GCT)  purA → adenylosuccinate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,394,8750GT100.0% 954.2 / NA 285A124A (GCG→GCT) purAadenylosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (137/148);  total (137/148)

Only 100 of 285 total aligned reads displayed.

GTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCGAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCA  >  CP009273/4394728‑4395018
                                                                                                                                                   |                                                                                                                                               
 tctcCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTaaa                                                                                                                                              <  2:827785/150‑1 (MQ=255)
  ctcCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTaagg                                                                                                                                             >  1:2169478/1‑148 (MQ=255)
  ctcCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAg                                                                                                                                             <  2:2769819/150‑1 (MQ=255)
  ctcCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAg                                                                                                                                             >  1:13747/1‑150 (MQ=255)
  ctcCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAg                                                                                                                                             <  2:3208227/150‑1 (MQ=255)
   tcCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGc                                                                                                                                            >  1:1881326/1‑150 (MQ=255)
      ggCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGAt                                                                                                                                         >  2:1007120/1‑150 (MQ=255)
      ggCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCACCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGAt                                                                                                                                         >  2:2480130/1‑150 (MQ=255)
             cTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGcaca                                                                                                                                  <  2:1623789/150‑2 (MQ=255)
                atgaaagagatgaaagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCaccacc                                                                                                                               >  2:1304762/1‑150 (MQ=255)
                atgaaagagatgaaagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCaccacc                                                                                                                               >  1:1542578/1‑150 (MQ=255)
                  gaaagagatgaaagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCgg                                                                                                                             >  2:2016696/1‑150 (MQ=255)
                     agagatgaaagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCg                                                                                                                          <  1:1638147/150‑1 (MQ=255)
                      gagatgaaagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGt                                                                                                                         >  1:469454/1‑150 (MQ=255)
                           gaaagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTAt                                                                                                                    <  2:337481/150‑1 (MQ=255)
                             aagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCg                                                                                                                  <  1:3046258/150‑1 (MQ=255)
                                aaCTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGc                                                                                                               >  1:52587/1‑150 (MQ=255)
                                  cTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCt                                                                                                             >  2:881551/1‑150 (MQ=255)
                                  cTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCt                                                                                                             >  1:2742945/1‑150 (MQ=255)
                                   tGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTCTCGGGCCTg                                                                                                            >  2:2967382/1‑150 (MQ=255)
                                     gAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCt                                                                                                          <  2:173129/150‑1 (MQ=255)
                                      aaGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCtt                                                                                                         >  1:2455298/1‑150 (MQ=255)
                                       aGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTa                                                                                                        >  2:1742573/1‑150 (MQ=255)
                                        gACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTAt                                                                                                       <  1:3433149/150‑1 (MQ=255)
                                         aCCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATg                                                                                                      >  2:1356649/1‑150 (MQ=255)
                                           cGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGaa                                                                                                    >  1:1397636/1‑150 (MQ=255)
                                           cGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGaa                                                                                                    >  1:1161849/1‑150 (MQ=255)
                                            gTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAg                                                                                                   >  2:1463218/1‑150 (MQ=255)
                                                  tCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAg                                                                                             <  1:3604942/150‑1 (MQ=255)
                                                      cGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGc                                                                                         >  1:2949172/1‑150 (MQ=255)
                                                      cGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGc                                                                                         >  2:3646343/1‑150 (MQ=255)
                                                      cGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGc                                                                                         >  2:1152952/1‑150 (MQ=255)
                                                       gTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCa                                                                                        >  1:3235293/1‑150 (MQ=255)
                                                       gTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCa                                                                                        <  2:3277132/150‑1 (MQ=255)
                                                         tCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACg                                                                                      <  2:1979271/150‑1 (MQ=255)
                                                            tGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTcg                                                                                   >  1:2605875/1‑150 (MQ=255)
                                                            tGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTcg                                                                                   <  2:3096411/150‑1 (MQ=255)
                                                            tGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTcg                                                                                   <  1:1214240/150‑1 (MQ=255)
                                                            tGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTcg                                                                                   <  1:3113647/150‑1 (MQ=255)
                                                             gAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACAGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTcgc                                                                                  >  2:837566/1‑150 (MQ=255)
                                                                cGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGt                                                                               >  2:2249586/1‑150 (MQ=255)
                                                                cGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGt                                                                               >  1:22645/1‑150 (MQ=255)
                                                                  tCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCt                                                                             <  2:2661100/150‑1 (MQ=255)
                                                                   ctgctgctgTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTg                                                                            <  2:3350949/150‑1 (MQ=255)
                                                                   ctgctgctgTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTg                                                                            <  1:1027058/150‑1 (MQ=255)
                                                                    tgctgctgTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGc                                                                           <  1:2474979/150‑1 (MQ=255)
                                                                     gctgctgTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCg                                                                          >  2:3673032/1‑150 (MQ=255)
                                                                     gctgctgTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCg                                                                          <  1:97050/150‑1 (MQ=255)
                                                                         ctgTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGtt                                                                      <  1:330914/150‑1 (MQ=255)
                                                                              tGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGa                                                                 >  1:2791274/1‑150 (MQ=255)
                                                                                aaGCAGGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGAcc                                                               <  1:2500208/150‑1 (MQ=255)
                                                                                 aGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCt                                                              >  2:6066/1‑150 (MQ=255)
                                                                                  gCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCtt                                                             <  1:1742573/150‑1 (MQ=255)
                                                                                   cATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCttt                                                            >  2:3010285/1‑150 (MQ=255)
                                                                                   cATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCttt                                                            >  1:2046528/1‑150 (MQ=255)
                                                                                    aTGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCtttt                                                           <  1:179627/150‑1 (MQ=255)
                                                                                     tGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTc                                                          <  1:1860166/150‑1 (MQ=255)
                                                                                     tGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTc                                                          <  1:3753624/150‑1 (MQ=255)
                                                                                         cGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACa                                                      <  2:3443731/150‑1 (MQ=255)
                                                                                           cTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACaaa                                                    <  1:484694/150‑1 (MQ=255)
                                                                                            tGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAg                                                   <  2:1042545/150‑1 (MQ=255)
                                                                                            tGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAg                                                   <  2:2140652/150‑1 (MQ=255)
                                                                                             gATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGa                                                  >  2:2503776/1‑150 (MQ=255)
                                                                                             gATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGa                                                  >  2:490306/1‑150 (MQ=255)
                                                                                              aTCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGaa                                                 <  2:2123853/150‑1 (MQ=255)
                                                                                              aTCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGaa                                                 >  2:1291647/1‑150 (MQ=255)
                                                                                                 cTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAAcc                                              >  1:348948/1‑150 (MQ=255)
                                                                                                  ttGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCt                                             >  2:2201011/1‑150 (MQ=255)
                                                                                                   tGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCtt                                            >  2:1819533/1‑150 (MQ=255)
                                                                                                    gATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTc                                           <  2:2742945/150‑1 (MQ=255)
                                                                                                     aTTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCg                                          >  1:976952/1‑150 (MQ=255)
                                                                                                     aTTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCg                                          >  2:1638297/1‑150 (MQ=255)
                                                                                                       tATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCt                                        <  2:1397636/150‑1 (MQ=255)
                                                                                                        aTCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTg                                       <  1:1086266/150‑1 (MQ=255)
                                                                                                        aTCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTg                                       >  2:1786192/1‑150 (MQ=255)
                                                                                                        aTCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTg                                       <  2:2722055/150‑1 (MQ=255)
                                                                                                               tGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACt                                <  2:565620/150‑1 (MQ=255)
                                                                                                                 cgcTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGa                              >  1:69181/1‑150 (MQ=255)
                                                                                                                   cTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGaaa                            <  1:1325937/150‑1 (MQ=255)
                                                                                                                     ggATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAaaga                          <  2:3637362/150‑1 (MQ=255)
                                                                                                                      gATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAaagaa                         <  1:1510581/150‑1 (MQ=255)
                                                                                                                         aaCGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTg                      <  2:61179/150‑1 (MQ=255)
                                                                                                                            gcgcgTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATg                   <  1:3021216/150‑1 (MQ=255)
                                                                                                                            gcgcgTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATg                   <  2:2320814/150‑1 (MQ=255)
                                                                                                                               cgTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGaa                <  2:771551/150‑1 (MQ=255)
                                                                                                                                gTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAat               >  2:1922968/1‑150 (MQ=255)
                                                                                                                                  gagaAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAatat             >  1:3414344/1‑150 (MQ=255)
                                                                                                                                    gaAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATca           >  2:1086359/1‑150 (MQ=255)
                                                                                                                                    gaAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGAGAAAGTTGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATca           >  2:2104524/1‑150 (MQ=255)
                                                                                                                                    gaAAGCGCGTGGCGCTAAAGCGATCGGCACCACAGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATca           <  1:837566/150‑1 (MQ=255)
                                                                                                                                      aaGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATcaca         <  1:611271/150‑1 (MQ=255)
                                                                                                                                      aaGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATcaca         >  1:1271098/1‑150 (MQ=255)
                                                                                                                                       aGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACaa        >  2:1582075/1‑150 (MQ=255)
                                                                                                                                        gcgcGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATTGAATATCACAAc       <  2:2522689/150‑1 (MQ=255)
                                                                                                                                        gcgcGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAAc       <  2:2949172/150‑1 (MQ=255)
                                                                                                                                          gcgTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACtt     >  1:2420297/1‑150 (MQ=255)
                                                                                                                                           cgTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTc    >  1:628053/1‑150 (MQ=255)
                                                                                                                                           cgTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTc    <  2:1569632/150‑1 (MQ=255)
                                                                                                                                            gTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTcc   <  1:950019/150‑1 (MQ=255)
                                                                                                                                             tGGCGATAAAGCGATCGGCACCAACGGTCGTGGTATCGGGCCTTCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCa  <  2:2454602/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCGAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCA  >  CP009273/4394728‑4395018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑