Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,405,730 G→T intergenic (+13/+104) aidB → / ← yjfN DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase/DUF1471 family periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,405,7300GT100.0% 840.8 / NA 252intergenic (+13/+104)aidB/yjfNDNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase/DUF1471 family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (127/125);  total (127/125)

Only 100 of 252 total aligned reads displayed.

TGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGC  >  CP009273/4405584‑4405876
                                                                                                                                                  |                                                                                                                                                  
  ttGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCg                                                                                                                                               <  1:2831154/150‑1 (MQ=255)
  ttGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCg                                                                                                                                               >  1:1812586/1‑150 (MQ=255)
     aaaTATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACg                                                                                                                                            <  1:2814810/150‑1 (MQ=255)
       atatGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCt                                                                                                                                          <  2:3776754/150‑1 (MQ=255)
        tatGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCTCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTg                                                                                                                                         <  1:3500634/150‑1 (MQ=255)
         atGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTgg                                                                                                                                        <  2:436215/150‑1 (MQ=255)
           gcgtctcCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGTATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGtt                                                                                                                                      <  2:1904552/147‑1 (MQ=255)
           gCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGtt                                                                                                                                      <  1:358408/150‑1 (MQ=255)
           gCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGtt                                                                                                                                      >  1:1994618/1‑150 (MQ=255)
            cTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGttt                                                                                                                                     <  2:720642/150‑1 (MQ=255)
            cTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGttt                                                                                                                                     <  1:1708861/150‑1 (MQ=255)
             ttCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTc                                                                                                                                    <  1:1325036/150‑1 (MQ=255)
                tcCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGAt                                                                                                                                 <  2:2997245/150‑1 (MQ=255)
                 ccGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGAtt                                                                                                                                >  1:3670685/1‑150 (MQ=255)
                 ccGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGAtt                                                                                                                                <  2:1973066/150‑1 (MQ=255)
                 ccGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGAtt                                                                                                                                <  2:2320801/150‑1 (MQ=255)
                  cGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTa                                                                                                                               >  2:3325240/1‑150 (MQ=255)
                  cGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTa                                                                                                                               >  2:3347521/1‑150 (MQ=255)
                     cAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTaaat                                                                                                                            <  1:245475/150‑3 (MQ=255)
                     cAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACt                                                                                                                            >  2:2782099/1‑150 (MQ=255)
                        tGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTaaa                                                                                                                         >  2:1489375/1‑150 (MQ=255)
                        tGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTaaa                                                                                                                         >  1:554972/1‑150 (MQ=255)
                         ggCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAAt                                                                                                                        >  2:2062426/1‑150 (MQ=255)
                         ggCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAAt                                                                                                                        <  2:2566374/150‑1 (MQ=255)
                         ggCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAAt                                                                                                                        >  2:1213019/1‑150 (MQ=255)
                            gcAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGaa                                                                                                                     >  1:1411173/1‑150 (MQ=255)
                             cAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGaaa                                                                                                                    >  2:238910/1‑150 (MQ=255)
                              aGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAat                                                                                                                   >  2:3386140/1‑150 (MQ=255)
                              aGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAat                                                                                                                   >  2:1875674/1‑150 (MQ=255)
                                gCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAatat                                                                                                                 <  2:911570/150‑1 (MQ=255)
                                 cGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATAtg                                                                                                                <  1:3062949/150‑1 (MQ=255)
                                   tggtgTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATAtgtg                                                                                                              >  1:3792896/1‑150 (MQ=255)
                                    ggtgTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGa                                                                                                             <  2:1208161/150‑1 (MQ=255)
                                       gtCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGaaaa                                                                                                          >  1:971433/1‑150 (MQ=255)
                                        tCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAAt                                                                                                         >  1:2481756/1‑150 (MQ=255)
                                         cAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAAtt                                                                                                        <  2:1782626/150‑1 (MQ=255)
                                         cAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAAtt                                                                                                        >  1:702663/1‑150 (MQ=255)
                                          aGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTg                                                                                                       >  1:297226/1‑150 (MQ=255)
                                          aGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGAACCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTg                                                                                                       >  2:3421124/1‑150 (MQ=255)
                                               aTGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTACGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGc                                                                                                  >  2:2744728/1‑150 (MQ=255)
                                               aTGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGc                                                                                                  <  2:1036552/150‑1 (MQ=255)
                                                 gTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCg                                                                                                <  2:326770/150‑1 (MQ=255)
                                                   tAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGa                                                                                              >  1:3081082/1‑150 (MQ=255)
                                                   tAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGa                                                                                              <  1:3635593/150‑1 (MQ=255)
                                                    aGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGAc                                                                                             <  2:2349884/150‑1 (MQ=255)
                                                    aGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGAc                                                                                             <  2:515569/150‑1 (MQ=255)
                                                     gATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACa                                                                                            <  2:3288895/150‑1 (MQ=255)
                                                     gATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACa                                                                                            <  1:1565803/150‑1 (MQ=255)
                                                     gATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACa                                                                                            <  2:1240622/150‑1 (MQ=255)
                                                      aTACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACaa                                                                                           >  2:1149406/1‑150 (MQ=255)
                                                        aCGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAgg                                                                                         <  2:3483520/150‑1 (MQ=255)
                                                              ggcggcgTACTTTTGTCTGAGAAGTTCCAGAATGATTTTTTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTTTACGACTTATGCGACGCTGGTTTCGATTAAATAAATGAAATATGTTTATATTGTATGCCGTACAATGCGCTcg                                                                                   <  2:3657455/150‑1 (MQ=255)
                                                                 ggcgTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCgcgc                                                                                >  1:259683/1‑150 (MQ=255)
                                                                 ggcgTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCgcgc                                                                                >  1:2231089/1‑150 (MQ=255)
                                                                 ggcgTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCgcgc                                                                                >  1:2169874/1‑150 (MQ=255)
                                                                   cgTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGcgccg                                                                              >  2:3058047/1‑150 (MQ=255)
                                                                     tACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCa                                                                            <  1:1296201/150‑1 (MQ=255)
                                                                     tACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCa                                                                            <  2:2901729/150‑1 (MQ=255)
                                                                        ggTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATcc                                                                         <  2:1496217/150‑1 (MQ=255)
                                                                        ggTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATcc                                                                         >  2:2179918/1‑150 (MQ=255)
                                                                          ttGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCgg                                                                       <  1:3026290/150‑1 (MQ=255)
                                                                            gTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCa                                                                     >  1:3213024/1‑150 (MQ=255)
                                                                            gTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCa                                                                     >  1:3213199/1‑150 (MQ=255)
                                                                             tCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTTGTTTCGATTAACTAAATGCAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCAt                                                                    <  2:68271/150‑1 (MQ=255)
                                                                              cAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCAtt                                                                   >  1:3654592/1‑150 (MQ=255)
                                                                               agagCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTg                                                                  <  2:2454266/150‑1 (MQ=255)
                                                                                 agCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGtt                                                                <  2:3671221/150‑1 (MQ=255)
                                                                                   cAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCa                                                              >  2:2332978/1‑150 (MQ=255)
                                                                                    aGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCAt                                                             <  2:2649173/150‑1 (MQ=255)
                                                                                      aTCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCAttt                                                           <  2:1543974/150‑1 (MQ=255)
                                                                                         cAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCt                                                        <  2:777673/150‑1 (MQ=255)
                                                                                          aGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTg                                                       >  1:1377888/1‑150 (MQ=255)
                                                                                           gAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGt                                                      >  1:3342666/1‑150 (MQ=255)
                                                                                              tGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGa                                                   <  2:2717144/150‑1 (MQ=255)
                                                                                                aTTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATg                                                 <  2:1322467/150‑1 (MQ=255)
                                                                                                 tttATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGa                                                >  1:2873350/1‑150 (MQ=255)
                                                                                                  ttATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTAGATTAACTAAATGAAATATGTGAAAATTGTAGGCAGGACAAGGCGATCGCGCCGCATCCGGCATTGTTCTTTTGCTGTAGATGac                                               <  1:1904019/150‑1 (MQ=255)
                                                                                                     ttGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACt                                            <  1:2765270/150‑1 (MQ=255)
                                                                                                         tgcGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATg                                        <  1:12635/150‑1 (MQ=255)
                                                                                                         tgcGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATg                                        >  2:248253/1‑150 (MQ=255)
                                                                                                          gcGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGc                                       <  1:3393520/150‑1 (MQ=255)
                                                                                                          gcGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGc                                       <  1:1495755/150‑1 (MQ=255)
                                                                                                           cGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCa                                      >  1:3670235/1‑150 (MQ=255)
                                                                                                           cGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGAGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCa                                      >  2:3211714/1‑150 (MQ=255)
                                                                                                              gCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATAc                                   <  1:592476/150‑1 (MQ=255)
                                                                                                               cGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACa                                  <  2:3525438/150‑1 (MQ=255)
                                                                                                                gACGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAg                                 >  1:859242/1‑150 (MQ=255)
                                                                                                                 aCGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGt                                >  2:1893450/1‑150 (MQ=255)
                                                                                                                  cGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTa                               <  1:2701531/150‑1 (MQ=255)
                                                                                                                  cGGGGGGAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTa                               >  1:376235/1‑150 (MQ=255)
                                                                                                                   ggggggAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTAt                              >  1:2202164/1‑150 (MQ=255)
                                                                                                                    gggggAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATc                             >  2:1653278/1‑150 (MQ=255)
                                                                                                                      gggAGTGTGTGTGTAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATCGc                           >  2:3314519/1‑150 (MQ=255)
                                                                                                                          gtgtgtgtgtAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATCGCCTGt                       <  1:799458/150‑1 (MQ=255)
                                                                                                                             tgtgtgtAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATCGCCTGTAcg                    <  2:87683/150‑1 (MQ=255)
                                                                                                                                  gtAAGCGTATACGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCa               >  2:1597715/1‑150 (MQ=255)
                                                                                                                                          ataCGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACACATGCATACAGTATCGCCTGTACGCGCCAGTTATCAg       >  2:1428673/1‑150 (MQ=255)
                                                                                                                                           taCGACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAgg      >  1:946431/1‑150 (MQ=255)
                                                                                                                                              gACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGgctg   >  1:676185/1‑150 (MQ=255)
                                                                                                                                              gACTTATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGgctg   >  1:2581393/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGC  >  CP009273/4405584‑4405876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑