Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 337,720 G→T E295D (GAG→GAT)  yahJ → putative metallo‑dependent hydrolase domain deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273337,7200GT99.5% 681.9 / ‑5.1 207E295D (GAG→GAT) yahJputative metallo‑dependent hydrolase domain deaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (112/94);  minor base C (0/1);  total (112/95)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 208 total aligned reads displayed.

CCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCA  >  CP009273/337573‑337866
                                                                                                                                                   |                                                                                                                                                  
cccGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATaa                                                                                                                                                  <  2:2283695/150‑1 (MQ=255)
  cGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATaaaa                                                                                                                                                <  2:966987/150‑1 (MQ=255)
  cGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATaaaa                                                                                                                                                >  2:1610941/1‑150 (MQ=255)
  cGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATaaaa                                                                                                                                                <  1:3583187/150‑1 (MQ=255)
      cAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGcc                                                                                                                                            >  1:11538/1‑150 (MQ=255)
      cAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGcc                                                                                                                                            >  2:2567312/1‑150 (MQ=255)
        gtgtTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCcac                                                                                                                                          >  2:3060769/1‑150 (MQ=255)
         tgtTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCcaca                                                                                                                                         <  2:3620375/150‑1 (MQ=255)
            tGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCt                                                                                                                                      >  1:1038090/1‑150 (MQ=255)
            tGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCt                                                                                                                                      <  1:635973/150‑1 (MQ=255)
                 gcgcGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAgg                                                                                                                                 <  2:1555996/150‑1 (MQ=255)
                  cgcgATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAggg                                                                                                                                >  2:1772983/1‑150 (MQ=255)
                  cgcgATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAggg                                                                                                                                <  1:750876/150‑1 (MQ=255)
                  cgcgATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAggg                                                                                                                                >  1:1861633/1‑150 (MQ=255)
                   gcgATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGc                                                                                                                               <  1:2106970/150‑1 (MQ=255)
                      aTGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAg                                                                                                                            <  2:1970641/150‑1 (MQ=255)
                      aTGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAg                                                                                                                            <  1:1632971/150‑1 (MQ=255)
                       tGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGc                                                                                                                           >  1:734854/1‑150 (MQ=255)
                         gAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTg                                                                                                                         >  2:640897/1‑150 (MQ=255)
                          aaaaaTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGa                                                                                                                        >  1:2311097/1‑150 (MQ=255)
                          aaaaaTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGa                                                                                                                        <  1:682522/150‑1 (MQ=255)
                          aaaaaTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGa                                                                                                                        >  1:517420/1‑150 (MQ=255)
                          aaaaaTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGa                                                                                                                        >  2:3561544/1‑150 (MQ=255)
                            aaaTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGAcc                                                                                                                      >  1:3331848/1‑150 (MQ=255)
                              aTCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCAt                                                                                                                    >  2:2751483/1‑150 (MQ=255)
                                 ccTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAg                                                                                                                 <  1:3025603/150‑1 (MQ=255)
                                    cGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCa                                                                                                              <  2:825414/150‑1 (MQ=255)
                                    cGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCa                                                                                                              <  2:826798/150‑1 (MQ=255)
                                     gACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCAc                                                                                                             >  2:1935676/1‑150 (MQ=255)
                                        acCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGcc                                                                                                          >  2:1591242/1‑150 (MQ=255)
                                         ccATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCt                                                                                                         >  1:2448405/1‑150 (MQ=255)
                                          cATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCtt                                                                                                        >  2:3760293/1‑150 (MQ=255)
                                          cATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCtt                                                                                                        >  2:3126912/1‑150 (MQ=255)
                                           aTGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCttt                                                                                                       <  1:250882/150‑1 (MQ=255)
                                            tGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTg                                                                                                      <  2:316511/150‑1 (MQ=255)
                                             gTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTgc                                                                                                     >  1:822494/1‑150 (MQ=255)
                                             gTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTgc                                                                                                     >  1:142178/1‑150 (MQ=255)
                                              ttCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTgcg                                                                                                    <  2:277879/150‑1 (MQ=255)
                                               tCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACCGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTgcgc                                                                                                   <  2:2625020/150‑1 (MQ=255)
                                                 cAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTg                                                                                                 <  2:1474456/150‑1 (MQ=255)
                                                          cTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTc                                                                                        <  1:167289/150‑1 (MQ=255)
                                                          cTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTc                                                                                        <  1:399725/150‑1 (MQ=255)
                                                            ggACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCaa                                                                                      >  2:3254833/1‑150 (MQ=255)
                                                            ggACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCaa                                                                                      >  2:1217660/1‑150 (MQ=255)
                                                             gACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAAc                                                                                     >  2:691903/1‑150 (MQ=255)
                                                               cTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGa                                                                                   <  1:1434640/150‑1 (MQ=255)
                                                                   gACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCac                                                                               >  1:2923908/1‑149 (MQ=255)
                                                                   gACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCaa                                                                               >  1:80380/1‑150 (MQ=255)
                                                                            gTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGAt                                                                      >  2:147821/1‑150 (MQ=255)
                                                                               gATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGaa                                                                   <  2:3641562/150‑1 (MQ=255)
                                                                                  atTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTg                                                                <  2:2473635/150‑1 (MQ=255)
                                                                                   ttCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTgg                                                               <  1:257600/150‑1 (MQ=255)
                                                                                   ttCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTgg                                                               >  1:2099530/1‑150 (MQ=255)
                                                                                    taacatGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTATATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGc                                                              >  1:1973859/6‑150 (MQ=255)
                                                                                       ccTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGaa                                                           <  2:639160/150‑1 (MQ=255)
                                                                                        cTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAAc                                                          <  2:814655/150‑1 (MQ=255)
                                                                                         tGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAAcc                                                         <  2:1272279/150‑1 (MQ=255)
                                                                                           cACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCgg                                                       >  2:1155614/1‑150 (MQ=255)
                                                                                           cACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCgg                                                       <  1:2905176/150‑1 (MQ=255)
                                                                                             cGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAt                                                     <  1:2722523/150‑1 (MQ=255)
                                                                                             cGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAt                                                     <  2:296149/150‑1 (MQ=255)
                                                                                             cGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAt                                                     >  2:2990814/1‑150 (MQ=255)
                                                                                              gAAACCACTCCGGCAGGCGTGGCAGCCCTCAATGATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCACCAGGTAGATGACCTGGCGAACCGGagg                                                    >  2:717921/1‑148 (MQ=255)
                                                                                                  ccACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGAtggtgg                                                >  1:3717754/1‑150 (MQ=255)
                                                                                                   cACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATggtggt                                               >  1:1100460/1‑150 (MQ=255)
                                                                                                    aCTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGgtggtg                                              <  2:564926/150‑1 (MQ=255)
                                                                                                     cTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGc                                             <  2:2311097/150‑1 (MQ=255)
                                                                                                      tCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGca                                            >  2:2520594/1‑150 (MQ=255)
                                                                                                       ccGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGcaa                                           <  1:1772983/150‑1 (MQ=255)
                                                                                                         ggCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGcaaca                                         >  1:2213387/1‑150 (MQ=255)
                                                                                                         ggCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGcaaca                                         <  2:1231020/150‑1 (MQ=255)
                                                                                                               cGTGGCAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTc                                   >  2:968139/1‑150 (MQ=255)
                                                                                                                    cAGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCg                              >  2:1359343/1‑150 (MQ=255)
                                                                                                                     aGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGc                             >  2:2168824/1‑150 (MQ=255)
                                                                                                                     aGCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGc                             >  1:2528359/1‑150 (MQ=255)
                                                                                                                      gCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGcc                            >  2:2135751/1‑150 (MQ=255)
                                                                                                                      gCCATCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGcc                            >  1:3470665/1‑150 (MQ=255)
                                                                                                                         aTCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCg                         >  2:1829221/1‑150 (MQ=255)
                                                                                                                         aTCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCg                         <  2:618039/150‑1 (MQ=255)
                                                                                                                         aTCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCg                         >  1:3138266/1‑150 (MQ=255)
                                                                                                                          tCAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGa                        <  1:3622854/150‑1 (MQ=255)
                                                                                                                           cAATTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGAc                       >  1:1167518/1‑150 (MQ=255)
                                                                                                                            aaTTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACg                      <  1:474972/150‑1 (MQ=255)
                                                                                                                            aaTTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACg                      <  2:142178/150‑1 (MQ=255)
                                                                                                                            aaTTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACg                      >  1:3392434/1‑150 (MQ=255)
                                                                                                                             aTTATATGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACgg                     >  2:575799/1‑150 (MQ=255)
                                                                                                                                atatGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGc                  <  1:2089642/150‑1 (MQ=255)
                                                                                                                                 tatGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGcc                 >  1:2788926/1‑150 (MQ=255)
                                                                                                                                   tGGTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGa               >  2:3459837/1‑150 (MQ=255)
                                                                                                                                    ggTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGAt              >  2:1111949/1‑150 (MQ=255)
                                                                                                                                     gTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGAtt             >  2:3331705/1‑150 (MQ=255)
                                                                                                                                     gTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGAtt             >  1:2454339/1‑150 (MQ=255)
                                                                                                                                     gTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGAtt             >  2:1583020/1‑150 (MQ=255)
                                                                                                                                     gTTGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGAtt             >  1:3414653/1‑150 (MQ=255)
                                                                                                                                      ttGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTg            >  1:2396655/1‑150 (MQ=255)
                                                                                                                                       tGAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTgg           >  2:3374781/1‑150 (MQ=255)
                                                                                                                                        gAAACGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACAATCAGTCACGTCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGATCTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGc          <  1:3199281/150‑1 (MQ=255)
                                                                                                                                         aaaCGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCa         >  1:1980295/1‑150 (MQ=255)
                                                                                                                                         aaaCGGTAGATAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCa         >  1:904040/1‑150 (MQ=255)
                                                                                                                                                agatAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCa  >  1:1745425/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCAATTATATGGTTGAAACGGTAGAGAAAACGCCACAGCTGAAGGGCAAGCTGACCATCAGTCACGCCTTTGCGCTGGCAACGCTCAACGAGCAACAGGTAGATGAACTGGCGAACCGGATGGTGGTGCAACAAATTTCTATCGCCTCGACGGTGCCGATTGGCACGCTGCA  >  CP009273/337573‑337866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑