BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,494,721	G→A	A283T (GCC→ACC)	yjhB →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,494,721	0	G	A	99.6%	741.1 / NA	228	A283T (GCC→ACC)	yjhB	putative transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (104/122); minor base T (1/0); total (106/122)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

CCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTA  >  CP009273/4494574‑4494865

 cGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCt                                                                                                                                                <  2:1957280/150‑1 (MQ=255)

   aTAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCttt                                                                                                                                              <  1:864487/150‑1 (MQ=255)

    tAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTg                                                                                                                                             <  2:1932239/150‑1 (MQ=255)

     aaaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGt                                                                                                                                            >  2:830763/1‑150 (MQ=255)

     aaaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGt                                                                                                                                            <  1:60162/150‑1 (MQ=255)

        cGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGt                                                                                                                                         <  2:2997253/150‑1 (MQ=255)

         ggACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTc                                                                                                                                        <  1:2368152/150‑1 (MQ=255)

          gACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCg                                                                                                                                       >  1:1814769/1‑150 (MQ=255)

             tactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTc                                                                                                                                    >  1:2145451/1‑150 (MQ=255)

              actTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCt                                                                                                                                   <  2:1723126/150‑1 (MQ=255)

                    ttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAAc                                                                                                                             >  2:2291153/1‑150 (MQ=255)

                     tcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACt                                                                                                                            <  1:1223673/150‑1 (MQ=255)

                     tcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACt                                                                                                                            <  1:3596390/150‑1 (MQ=255)

                      cctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACtt                                                                                                                           >  2:3440181/1‑150 (MQ=255)

                       ctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTc                                                                                                                          <  2:892661/150‑1 (MQ=255)

                          ccTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCatt                                                                                                                       <  1:3165497/150‑1 (MQ=255)

                           cTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCattt                                                                                                                      <  1:1447337/150‑1 (MQ=255)

                           cTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCattt                                                                                                                      <  2:3733427/150‑1 (MQ=255)

                             ggCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCatttat                                                                                                                    <  1:1749079/150‑1 (MQ=255)

                                aGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTAtttt                                                                                                                 >  2:441819/1‑150 (MQ=255)

                                  aTAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGTTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCTGTCTAATAAATTAATTTAttttac                                                                                                               >  1:2006110/1‑148 (MQ=255)

                                   tAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCt                                                                                                              >  2:941647/1‑150 (MQ=255)

                                    aaTGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCtg                                                                                                             >  1:3432436/1‑149 (MQ=255)

                                     aTGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCttt                                                                                                            <  2:1586405/150‑1 (MQ=255)

                                      tGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTg                                                                                                           >  1:757394/1‑150 (MQ=255)

                                         aGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTcc                                                                                                        >  2:3504651/1‑150 (MQ=255)

                                         aGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTcc                                                                                                        >  2:1056069/1‑150 (MQ=255)

                                            tAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATA‑TTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑tt                                                                                                   >  2:2950450/1‑150 (MQ=255)

                                             aaTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑tt                                                                                                   >  2:2377396/1‑150 (MQ=255)

                                               tACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑tttt                                                                                                 <  2:3629067/150‑1 (MQ=255)

                                                aCAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑ttttt                                                                                                <  2:3380198/150‑1 (MQ=255)

                                                aCAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑ttttt                                                                                                <  2:3779286/150‑1 (MQ=255)

                                                aCAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑ttttt                                                                                                <  2:2809717/150‑1 (MQ=255)

                                                aCAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑ttttt                                                                                                >  1:457481/1‑150 (MQ=255)

                                                  aGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑ttttttt                                                                                              <  2:3066981/150‑1 (MQ=255)

                                                    tGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTa                                                                                            <  2:3572338/150‑1 (MQ=255)

                                                      gTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTAtt                                                                                          <  2:3508556/150‑1 (MQ=255)

                                                        cATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTc                                                                                        <  1:2443038/150‑1 (MQ=255)

                                                         aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCt                                                                                       >  1:261564/1‑150 (MQ=255)

                                                         aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCt                                                                                       <  1:2138199/150‑1 (MQ=255)

                                                         aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCt                                                                                       <  2:2725269/150‑1 (MQ=255)

                                                            tCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCtgtg                                                                                    >  1:79818/1‑150 (MQ=255)

                                                                  ctGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAAc                                                                              >  1:1649838/1‑150 (MQ=255)

                                                                    gatgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAAct                                                                             >  2:2493981/1‑150 (MQ=255)

                                                                    gatgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTTATTTCTGTGAAAAAct                                                                             <  1:535998/150‑1 (MQ=255)

                                                                     atgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAActct                                                                           >  1:2563481/1‑150 (MQ=255)

                                                                      tgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAActctt                                                                          >  2:98979/1‑150 (MQ=255)

                                                                       gaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAActcttc                                                                         >  1:1968594/1‑150 (MQ=255)

                                                                        aCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAActcttct                                                                        <  2:793118/150‑1 (MQ=255)

                                                                          aaTAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAActcttctct                                                                      <  1:3438617/150‑1 (MQ=255)

                                                                             aGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTtat                                                                   >  2:556663/1‑150 (MQ=255)

                                                                             aGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTtat                                                                   >  1:141190/1‑150 (MQ=255)

                                                                             aGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTtat                                                                   <  2:2051551/150‑1 (MQ=255)

                                                                             aGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTtat                                                                   <  2:2042535/150‑1 (MQ=255)

                                                                              gCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTtata                                                                  <  1:1559373/150‑1 (MQ=255)

                                                                              gCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTtata                                                                  <  2:322466/150‑1 (MQ=255)

                                                                               cAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAg                                                                 <  1:3117469/150‑1 (MQ=255)

                                                                                    ttAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTa                                                            <  2:197272/150‑1 (MQ=255)

                                                                                     tAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTAt                                                           <  2:2147509/150‑1 (MQ=255)

                                                                                      aGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAGAAACTCTTCTCTTATAGGATTATg                                                          >  1:108375/1‑150 (MQ=255)

                                                                                      aGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATg                                                          >  1:2782761/1‑150 (MQ=255)

                                                                                        gAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtc                                                        <  1:1502027/150‑1 (MQ=255)

                                                                                         aaCACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtct                                                       >  1:3361540/1‑150 (MQ=255)

                                                                                             cTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCttt                                                   <  1:499806/150‑1 (MQ=255)

                                                                                             cTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCttt                                                   <  1:2647736/150‑1 (MQ=255)

                                                                                             cTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCttt                                                   <  1:1890072/150‑1 (MQ=255)

                                                                                              tGACAGGTACAATATTTTTTGGTTTTGATTGTGATAAGATTGGTGT‑AAAAAAAACATTTGTAGTCGGTCTAATAACTTCAAATATTATCCATTGTCCTC‑TTTTATTTATATCTTTGAAAAACTCATCTCTAATAAGATAAAGTCTCTTTg                                                  >  1:2925075/1‑150 (MQ=255)

                                                                                               gACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTgg                                                 >  1:1272903/1‑150 (MQ=255)

                                                                                               gACAGGTACAATATTTTTTGGTTGTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCGTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTgg                                                 <  2:2645438/150‑1 (MQ=255)

                                                                                                 cAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGAt                                               >  1:3750316/1‑150 (MQ=255)

                                                                                                 cAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTATTTGGAt                                               >  2:3043248/1‑150 (MQ=255)

                                                                                                    gTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATATGATTATTTCTCTTTGGATTaa                                            >  1:415427/1‑150 (MQ=255)

                                                                                                     tACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAAt                                           >  2:1523187/1‑150 (MQ=255)

                                                                                                      aCAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATg                                          <  1:1021371/150‑1 (MQ=255)

                                                                                                         atatTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGttt                                       >  2:2860884/1‑150 (MQ=255)

                                                                                                         atatTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGttt                                       >  1:1887336/1‑150 (MQ=255)

                                                                                                         atatTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGttt                                       <  1:2324404/150‑1 (MQ=255)

                                                                                                         atatTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGttt                                       <  2:1814770/150‑1 (MQ=255)

                                                                                                         atatTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGttt                                       <  2:1692956/150‑1 (MQ=255)

                                                                                                             tttttGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACaa                                   >  2:2892115/1‑150 (MQ=255)

                                                                                                             tttttGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACaa                                   >  2:2597870/1‑150 (MQ=255)

                                                                                                             tttttGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACaa                                   <  2:3160788/150‑1 (MQ=255)

                                                                                                                 tGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAAttt                               >  1:2255177/1‑150 (MQ=255)

                                                                                                                  ggTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTa                              <  1:999218/150‑1 (MQ=255)

                                                                                                                    ttttGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAgg                            <  2:150264/150‑1 (MQ=255)

                                                                                                                        gttgGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAtt                        <  2:669873/150‑1 (MQ=255)

                                                                                                                        gttgGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAtt                        <  1:103680/150‑1 (MQ=255)

                                                                                                                             tGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAgg                   >  1:1033596/1‑150 (MQ=255)

                                                                                                                             tGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAgg                   >  1:286360/1‑150 (MQ=255)

                                                                                                                             tGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAgg                   >  1:285911/1‑150 (MQ=255)

                                                                                                                                 aaGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttg               <  2:1968594/150‑1 (MQ=255)

                                                                                                                                 aaGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttg               <  2:49553/150‑1 (MQ=255)

                                                                                                                                 aaGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttg               <  2:1221791/150‑1 (MQ=255)

                                                                                                                                 aaGATAGGTGT‑AAAAAAAACCTATGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTAAAAATTTATGTATTGCattggtg               >  2:1306347/1‑144 (MQ=255)

                                                                                                                                   gATTGGTGTAAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttgg              >  1:1933844/1‑150 (MQ=255)

                                                                                                                                    aTTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggtt            <  2:2573557/150‑1 (MQ=255)

                                                                                                                                    aTTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggtt            <  2:807826/150‑1 (MQ=255)

                                                                                                                                    aTTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggtt            >  1:2987130/1‑150 (MQ=255)

                                                                                                                                          gt‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCaaaa      >  2:14584/1‑150 (MQ=255)

                                                                                                                                           t‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAAt     >  1:661169/1‑150 (MQ=255)

CCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTA  >  CP009273/4494574‑4494865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`