BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,533,538	A→T	intergenic (+58/‑7)	fimA → / → fimI	major type 1 subunit fimbrin (pilin)/fimbrial protein involved in type 1 pilus biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,533,538	0	A	T	100.0%	830.7 / NA	247	intergenic (+58/‑7)	fimA/fimI	major type 1 subunit fimbrin (pilin)/fimbrial protein involved in type 1 pilus biosynthesis
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (113/134); total (113/134)

Only 100 of 249 total aligned reads displayed.

TTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAG  >  CP009273/4533392‑4533683

gtCCGGTCCAGGCGCGTTATTGTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAAGGCGGATGCGACCTTCAAGGTTCAGTATCAAGAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAtata                                                                                                                                                <  1:1047002/149‑1 (MQ=255)

 tCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATaa                                                                                                                                               >  1:1630641/1‑150 (MQ=255)

  ccGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAAc                                                                                                                                              <  1:2260770/150‑1 (MQ=255)

   cGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACg                                                                                                                                             <  2:2420337/150‑1 (MQ=255)

     ttCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGAt                                                                                                                                           <  2:2044000/150‑1 (MQ=255)

     ttCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGAt                                                                                                                                           <  2:3551494/150‑1 (MQ=255)

      tCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATg                                                                                                                                          >  2:3591878/1‑150 (MQ=255)

        cAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGaa                                                                                                                                        >  2:3379458/1‑150 (MQ=255)

         aGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGaaa                                                                                                                                       >  2:608862/1‑150 (MQ=255)

         aGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGaaa                                                                                                                                       <  1:1751982/150‑1 (MQ=255)

          ggCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGaaaa                                                                                                                                      <  2:2457367/150‑1 (MQ=255)

           gcgcGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAg                                                                                                                                     <  1:3019355/150‑1 (MQ=255)

            cgcgTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAgg                                                                                                                                    >  1:344650/1‑150 (MQ=255)

             gcgTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGa                                                                                                                                   <  1:2581369/150‑1 (MQ=255)

              cgTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGaa                                                                                                                                  <  2:2742581/150‑1 (MQ=255)

              cgTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGaa                                                                                                                                  <  1:1228336/150‑1 (MQ=255)

                 tATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAagag                                                                                                                               >  2:1657904/1‑150 (MQ=255)

                 tATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAagag                                                                                                                               <  1:1269759/150‑1 (MQ=255)

                  aTTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAgaga                                                                                                                              >  2:1770469/1‑150 (MQ=255)

                    tttGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGatt                                                                                                                            <  1:1192152/150‑1 (MQ=255)

                     ttGCAACCGGGGCCGCAACACCGGGTGCTGCTAATGCGGATGCGAACTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTTCGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGatta                                                                                                                           <  1:1850667/150‑1 (MQ=255)

                      tGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGattat                                                                                                                          <  1:96297/150‑1 (MQ=255)

                      tGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGattat                                                                                                                          <  1:2729335/150‑1 (MQ=255)

                        cAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTAttt                                                                                                                        >  1:749262/1‑150 (MQ=255)

                        cAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTAttt                                                                                                                        <  1:3157895/150‑1 (MQ=255)

                            cGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTAt                                                                                                                    <  2:3458435/150‑1 (MQ=255)

                             ggggCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTAtt                                                                                                                   <  2:1387719/150‑1 (MQ=255)

                             ggggCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTAtt                                                                                                                   <  1:3547531/150‑1 (MQ=255)

                             ggggCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTAtt                                                                                                                   <  2:2484508/150‑1 (MQ=255)

                              gggCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTa                                                                                                                  <  1:3396625/150‑1 (MQ=255)

                                 ccGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGcg                                                                                                               <  1:48670/150‑1 (MQ=255)

                                 ccGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGcg                                                                                                               <  1:1959340/150‑1 (MQ=255)

                                  cGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGcgt                                                                                                              >  2:1136607/1‑150 (MQ=255)

                                  cGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGcgt                                                                                                              >  2:1811726/1‑150 (MQ=255)

                                    cAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGcgtcg                                                                                                            >  2:1040712/1‑150 (MQ=255)

                                    cAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGcgtcg                                                                                                            >  2:2191224/1‑150 (MQ=255)

                                     aaCCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGcgtcgt                                                                                                           >  1:485585/1‑150 (MQ=255)

                                        cccGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTtgc                                                                                                        >  2:2115445/1‑150 (MQ=255)

                                         ccGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTtgct                                                                                                       <  2:3416413/150‑1 (MQ=255)

                                          cGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTtgctg                                                                                                      <  1:3073491/150‑1 (MQ=255)

                                          cGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTtgctg                                                                                                      <  1:1781414/150‑1 (MQ=255)

                                           gggTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTtgctgc                                                                                                     >  1:3349365/1‑150 (MQ=255)

                                            ggTGCTGCTAATGCGGATGCGACCTTCAAGGTTCGGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGcc                                                                                                    >  2:1541433/1‑150 (MQ=255)

                                            ggTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGcc                                                                                                    >  2:3437345/1‑150 (MQ=255)

                                            ggTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGcc                                                                                                    <  2:3571658/150‑1 (MQ=255)

                                            ggTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGcc                                                                                                    >  2:1417679/1‑150 (MQ=255)

                                              tgctgcTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCaa                                                                                                  >  1:445062/1‑150 (MQ=255)

                                              tgctgcTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCaa                                                                                                  >  1:444910/1‑150 (MQ=255)

                                               gctgctAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAAt                                                                                                 >  1:232838/1‑150 (MQ=255)

                                                 tgctAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGt                                                                                               >  1:1115577/1‑150 (MQ=255)

                                                       tGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGctc                                                                                         <  1:3694857/150‑1 (MQ=255)

                                                       tGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGctc                                                                                         <  1:2067726/150‑1 (MQ=255)

                                                        gCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGctct                                                                                        <  1:1411120/150‑1 (MQ=255)

                                                          ggATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTgg                                                                                      >  2:3055625/1‑150 (MQ=255)

                                                          ggATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTgg                                                                                      >  2:2869468/1‑150 (MQ=255)

                                                            aTGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGcc                                                                                    <  1:269484/150‑1 (MQ=255)

                                                             tGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCg                                                                                   >  1:1801682/1‑150 (MQ=255)

                                                                   cTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaaata                                                                             >  1:757840/1‑150 (MQ=255)

                                                                      cAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaaataaat                                                                          >  2:2703107/1‑150 (MQ=255)

                                                                        aGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATgg                                                                        <  2:1722684/150‑1 (MQ=255)

                                                                        aGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATgg                                                                        >  1:2978503/1‑150 (MQ=255)

                                                                        aGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATgg                                                                        <  2:2831175/150‑1 (MQ=255)

                                                                        aGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATgg                                                                        <  1:3602401/150‑1 (MQ=255)

                                                                           ttCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGAGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAAt                                                                     <  1:2339045/150‑1 (MQ=255)

                                                                              aGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATAcc                                                                  >  1:1579663/1‑150 (MQ=255)

                                                                               gTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCa                                                                 >  2:485247/1‑150 (MQ=255)

                                                                               gTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCa                                                                 >  2:2197443/1‑150 (MQ=255)

                                                                                tATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCAc                                                                <  2:3745994/150‑1 (MQ=255)

                                                                                   cAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGtt                                                             >  1:1000167/1‑150 (MQ=255)

                                                                                       aaCCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGccc                                                         >  2:3776344/1‑150 (MQ=255)

                                                                                       aaCCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGccc                                                         <  1:319218/150‑1 (MQ=255)

                                                                                       aaCCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGccc                                                         <  1:1507511/150‑1 (MQ=255)

                                                                                       aaCCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGccc                                                         >  2:1882903/1‑150 (MQ=255)

                                                                                        aCCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCcg                                                        <  1:1637870/150‑1 (MQ=255)

                                                                                             cccAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGa                                                   >  1:1218193/1‑150 (MQ=255)

                                                                                              ccAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGaa                                                  >  1:568235/1‑150 (MQ=255)

                                                                                              ccAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGaa                                                  <  1:2191462/150‑1 (MQ=255)

                                                                                                  gTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAatat                                              <  2:577737/150‑1 (MQ=255)

                                                                                                  gTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAatat                                              <  2:3349365/150‑1 (MQ=255)

                                                                                                   ttCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATg                                             >  1:2830056/1‑150 (MQ=255)

                                                                                                    tCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGc                                            <  2:3199463/150‑1 (MQ=255)

                                                                                                    tCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGc                                            <  1:3636257/150‑1 (MQ=255)

                                                                                                    tCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGc                                            <  2:1469244/150‑1 (MQ=255)

                                                                                                    tCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGc                                            <  1:3070727/150‑1 (MQ=255)

                                                                                                        ggACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAAtt                                        <  2:2594183/150‑1 (MQ=255)

                                                                                                           cGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCa                                     >  2:3038340/1‑150 (MQ=255)

                                                                                                              cATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAggg                                  >  2:717833/1‑150 (MQ=255)

                                                                                                              cATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAggg                                  <  1:3591878/150‑1 (MQ=255)

                                                                                                                  aCGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTc                              >  1:2999555/1‑150 (MQ=255)

                                                                                                                   cGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCa                             <  2:568235/150‑1 (MQ=255)

                                                                                                                        aGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCattatt                        >  2:89350/1‑150 (MQ=255)

                                                                                                                         gggATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTg                       <  2:3396875/150‑1 (MQ=255)

                                                                                                                         gggATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTg                       <  1:590079/150‑1 (MQ=255)

                                                                                                                          ggATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGc                      <  2:1705568/150‑1 (MQ=255)

                                                                                                                          ggATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGc                      <  2:1630641/150‑1 (MQ=255)

                                                                                                                              gCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGaa                  <  1:797565/150‑1 (MQ=255)

                                                                                                                                 cACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACt               <  2:752378/150‑1 (MQ=255)

                                                                                                                                          gCGATAAATATAACGATTAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGTCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCTGCGGAAATATGAAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGAtt      <  1:2616270/150‑1 (MQ=255)

                                                                                                                                           cGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTg     >  1:1460720/1‑150 (MQ=255)

                                                                                                                                             aTAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGaa   <  2:485720/150‑1 (MQ=255)

TTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAG  >  CP009273/4533392‑4533683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`