Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,536,446 A→T K512* (AAG→TAG)  fimD → fimbrial usher outer membrane porin protein; FimCD chaperone‑usher

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,536,4460AT100.0% 666.5 / NA 200K512* (AAG→TAG) fimDfimbrial usher outer membrane porin protein; FimCD chaperone‑usher
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (98/102);  total (98/102)

Only 100 of 200 total aligned reads displayed.

TATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAG  >  CP009273/4536299‑4536592
                                                                                                                                                   |                                                                                                                                                  
tataACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAg                                                                                                                                                  <  1:3695699/150‑1 (MQ=255)
tataACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAg                                                                                                                                                  <  1:1338818/150‑1 (MQ=255)
  taACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGcc                                                                                                                                                >  2:1855255/1‑150 (MQ=255)
     cAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGaa                                                                                                                                             >  2:1166656/1‑150 (MQ=255)
     cAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGaa                                                                                                                                             >  2:523847/1‑150 (MQ=255)
     cAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGaa                                                                                                                                             <  1:1923183/150‑1 (MQ=255)
      aaaTCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGaaa                                                                                                                                            <  2:3277485/150‑1 (MQ=255)
      aaaTCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGaaa                                                                                                                                            <  1:1467987/150‑1 (MQ=255)
        aTCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAAtt                                                                                                                                          <  2:3550016/150‑1 (MQ=255)
         tCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTc                                                                                                                                         >  2:3464801/1‑150 (MQ=255)
           gCTCAATGAATCAGTCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCAc                                                                                                                                       <  1:2459869/150‑1 (MQ=255)
             taaaTGAATCAGGCACGAATATTCAGTTAGTGGGGTACCGTTATTCGATCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCg                                                                                                                                     <  1:281577/148‑1 (MQ=255)
              cAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGa                                                                                                                                    >  1:2130585/1‑150 (MQ=255)
               aaTGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGAc                                                                                                                                   <  2:793906/150‑1 (MQ=255)
                  gAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTAt                                                                                                                                >  1:3399287/1‑150 (MQ=255)
                  gAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTAt                                                                                                                                >  2:555895/1‑150 (MQ=255)
                  gAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTAt                                                                                                                                >  2:692001/1‑150 (MQ=255)
                   aaTCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTAtt                                                                                                                               >  2:93220/1‑150 (MQ=255)
                    aTCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTa                                                                                                                              <  2:2667683/150‑1 (MQ=255)
                       aGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTCTTACaa                                                                                                                           >  2:1819793/1‑150 (MQ=255)
                         gCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAAcc                                                                                                                         <  1:2023453/150‑1 (MQ=255)
                         gCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAAcc                                                                                                                         <  1:1602299/150‑1 (MQ=255)
                            cGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCg                                                                                                                      >  1:279314/1‑150 (MQ=255)
                            cGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCg                                                                                                                      >  2:2415076/1‑150 (MQ=255)
                             gAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGc                                                                                                                     >  1:933089/1‑150 (MQ=255)
                              aaTATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCt                                                                                                                    >  1:1706352/1‑150 (MQ=255)
                               atatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCtt                                                                                                                   <  2:326536/150‑1 (MQ=255)
                                 atTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTtat                                                                                                                 >  1:1829652/1‑150 (MQ=255)
                                  ttCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTtata                                                                                                                <  2:2074330/150‑1 (MQ=255)
                                  ttCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTtata                                                                                                                <  2:1526195/150‑1 (MQ=255)
                                   tCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATaa                                                                                                               >  2:3688846/1‑150 (MQ=255)
                                       ttAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaaa                                                                                                           <  2:467276/150‑1 (MQ=255)
                                        tAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAc                                                                                                          <  1:378815/150‑1 (MQ=255)
                                         aGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcg                                                                                                         <  1:2326610/150‑1 (MQ=255)
                                         aGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcg                                                                                                         <  2:116470/150‑1 (MQ=255)
                                             ggTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCggg                                                                                                     >  2:3131439/1‑150 (MQ=255)
                                                 aCCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAAt                                                                                                 <  2:2217714/150‑1 (MQ=255)
                                                  ccGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAAtt                                                                                                <  2:3033021/150‑1 (MQ=255)
                                                    gTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCCCGCTTATAACAAACGCGGGAAATTAc                                                                                              <  2:87558/150‑1 (MQ=255)
                                                       aTTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAAc                                                                                           >  1:1165873/1‑150 (MQ=255)
                                                        ttCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACt                                                                                          <  2:728518/150‑1 (MQ=255)
                                                          cGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCa                                                                                        <  1:2632006/150‑1 (MQ=255)
                                                             ccAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATTGCTACAACATCGAAACACATGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAcca                                                                                     >  1:2862746/1‑149 (MQ=255)
                                                               aGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGtt                                                                                   >  2:3657008/1‑150 (MQ=255)
                                                                  ggATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACt                                                                                <  2:942200/150‑1 (MQ=255)
                                                                  ggATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACt                                                                                >  1:3739948/1‑150 (MQ=255)
                                                                  ggATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACt                                                                                >  1:2800062/1‑150 (MQ=255)
                                                                    atatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCa                                                                              >  2:1715101/1‑150 (MQ=255)
                                                                       ttttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAGCTCACCGTTACTCAGCa                                                                           >  1:2727461/1‑150 (MQ=255)
                                                                        tttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCaa                                                                          <  1:3620295/150‑1 (MQ=255)
                                                                        tttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCaa                                                                          <  2:3554548/150‑1 (MQ=255)
                                                                         ttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAAc                                                                         >  2:1732252/1‑150 (MQ=255)
                                                                          tAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACt                                                                        <  2:1430162/150‑1 (MQ=255)
                                                                            aTTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCg                                                                      >  1:3549266/1‑150 (MQ=255)
                                                                             tttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCgg                                                                     <  1:1308248/150‑1 (MQ=255)
                                                                              ttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCggg                                                                    >  2:3695308/1‑150 (MQ=255)
                                                                               tCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGgc                                                                   <  2:1200668/150‑1 (MQ=255)
                                                                                   tGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGcac                                                               >  2:161510/1‑150 (MQ=255)
                                                                                     aTACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACAt                                                             >  1:642901/1‑150 (MQ=255)
                                                                                     aTACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACAt                                                             <  2:3029380/150‑1 (MQ=255)
                                                                                     aTACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACAt                                                             >  1:2548632/1‑150 (MQ=255)
                                                                                         aacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAac                                                         <  1:797131/150‑1 (MQ=255)
                                                                                             tACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTg                                                     >  2:3316091/1‑150 (MQ=255)
                                                                                             tACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTg                                                     >  2:2916779/1‑150 (MQ=255)
                                                                                              aCAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGt                                                    >  1:485100/1‑150 (MQ=255)
                                                                                                 gTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTAtt                                                 <  1:124887/150‑1 (MQ=255)
                                                                                                  tCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTAttt                                                <  2:2944384/150‑1 (MQ=255)
                                                                                                   cGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTg                                               <  1:3012144/150‑1 (MQ=255)
                                                                                                    gaatgaatGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGa                                              >  1:3168001/1‑150 (MQ=255)
                                                                                                       tgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTg                                           >  1:1589455/1‑150 (MQ=255)
                                                                                                         aatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGt                                         >  2:1184432/1‑150 (MQ=255)
                                                                                                           tgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAg                                       >  2:3631297/1‑150 (MQ=255)
                                                                                                           tgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAg                                       >  2:1243124/1‑150 (MQ=255)
                                                                                                            ggCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGc                                      >  1:462377/1‑150 (MQ=255)
                                                                                                             gCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGcc                                     <  2:1002411/150‑1 (MQ=255)
                                                                                                             gCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGcc                                     <  1:918064/150‑1 (MQ=255)
                                                                                                                acaacaTCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATc                                  >  1:400462/1‑150 (MQ=255)
                                                                                                                     aTCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCCTCAAACt                             >  2:1979970/1‑150 (MQ=255)
                                                                                                                     aTCGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACt                             <  1:1855255/150‑1 (MQ=255)
                                                                                                                       cGAAACACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTa                           >  2:3142606/1‑150 (MQ=255)
                                                                                                                         aaaCACAGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTAtt                         <  1:161510/150‑1 (MQ=255)
                                                                                                                            cacaGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTggg                      >  1:851476/1‑150 (MQ=255)
                                                                                                                             acaGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTgggg                     <  1:648950/150‑1 (MQ=255)
                                                                                                                             acaGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTgggg                     <  1:1051140/150‑1 (MQ=255)
                                                                                                                              caGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGa                    <  2:1604429/150‑1 (MQ=255)
                                                                                                                               aGGACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGaa                   >  1:3664586/1‑150 (MQ=255)
                                                                                                                                ggACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGACATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAAc                  >  1:345217/1‑150 (MQ=255)
                                                                                                                                 gACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACg                 >  2:2449220/1‑150 (MQ=255)
                                                                                                                                 gACGGAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACg                 <  1:2415076/150‑1 (MQ=255)
                                                                                                                                    ggAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAGCTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGt              <  2:2727461/150‑1 (MQ=255)
                                                                                                                                    ggAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTAGAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGt              <  2:2130585/150‑1 (MQ=255)
                                                                                                                                     gAGTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTa             >  2:3660666/1‑150 (MQ=255)
                                                                                                                                       gTTATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAAt           <  2:3530278/150‑1 (MQ=255)
                                                                                                                                        ttATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATg          <  2:2619135/150‑1 (MQ=255)
                                                                                                                                         tATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGt         >  2:1493799/1‑150 (MQ=255)
                                                                                                                                         tATTCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGt         <  1:3142130/150‑1 (MQ=255)
                                                                                                                                           ttCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCg       <  1:536063/150‑1 (MQ=255)
                                                                                                                                            tCAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGa      <  2:408391/150‑1 (MQ=255)
                                                                                                                                             cAGGTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGAt     >  2:3001652/1‑150 (MQ=255)
                                                                                                                                               ggTTTAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGa   >  2:1347344/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAG  >  CP009273/4536299‑4536592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑