Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,567,278 G→T I166I (ATC→ATA)  mcrC ← 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,567,2780GT99.6% 772.7 / ‑6.3 232I166I (ATC→ATA) mcrC5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (120/111);  minor base C (1/0);  total (121/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

AGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCTT  >  CP009273/4567131‑4567424
                                                                                                                                                   |                                                                                                                                                  
aGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTAt                                                                                                                                                  >  2:3661492/1‑150 (MQ=255)
aGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTAt                                                                                                                                                  <  2:1129461/150‑1 (MQ=255)
   cGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAAc                                                                                                                                               <  2:725017/150‑1 (MQ=255)
      tAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGaa                                                                                                                                            <  1:129895/150‑1 (MQ=255)
       aaTTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCGTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAAt                                                                                                                                           >  2:2733353/1‑150 (MQ=255)
       aaTTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAAt                                                                                                                                           <  1:3761856/150‑1 (MQ=255)
        aTTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAAtt                                                                                                                                          >  2:589763/1‑150 (MQ=255)
        aTTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAAtt                                                                                                                                          <  1:1581523/150‑1 (MQ=255)
        aTTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAAtt                                                                                                                                          >  1:3268849/1‑150 (MQ=255)
         ttCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAAttt                                                                                                                                         <  2:3762138/150‑1 (MQ=255)
           cACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTtat                                                                                                                                       <  2:3159346/150‑1 (MQ=255)
             cgacgGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTataa                                                                                                                                     >  1:1584739/1‑150 (MQ=255)
              gacgGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTataat                                                                                                                                    >  2:3528282/1‑150 (MQ=255)
              gacgGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTataat                                                                                                                                    >  2:1633151/1‑150 (MQ=255)
              gacgGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTataat                                                                                                                                    >  2:2387659/1‑150 (MQ=255)
               acgGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTataata                                                                                                                                   <  2:1791910/150‑1 (MQ=255)
                cgGCAAAATTAATAAAGAAAATTTTGATAAATTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTAtaataa                                                                                                                                  <  2:1292654/150‑1 (MQ=255)
                   cAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAAcgc                                                                                                                               <  1:2734986/150‑1 (MQ=255)
                   cAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAAcgc                                                                                                                               <  1:1751068/150‑1 (MQ=255)
                     aaaTTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGt                                                                                                                             <  1:1960892/150‑1 (MQ=255)
                     aaaTTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGt                                                                                                                             <  2:2633692/150‑1 (MQ=255)
                         tCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTAttt                                                                                                                         <  1:2201631/150‑1 (MQ=255)
                          cATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTAtttt                                                                                                                        >  2:564517/1‑150 (MQ=255)
                             aaaGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTc                                                                                                                     >  2:3356449/1‑150 (MQ=255)
                             aaaGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTc                                                                                                                     >  2:3077051/1‑150 (MQ=255)
                               aGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCg                                                                                                                   <  1:230904/150‑1 (MQ=255)
                                 aaaCTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGcc                                                                                                                 >  2:3230847/1‑150 (MQ=255)
                                  aaCTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTCATAACGAATTTATAATAACGCGTATTTTTTCCGCCa                                                                                                                >  1:333836/1‑150 (MQ=255)
                                     ttttGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTc                                                                                                             >  2:1468080/1‑150 (MQ=255)
                                         gATAAAGTAATGACATCTCTTTTTCGTTCCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGAt                                                                                                         >  2:359621/1‑150 (MQ=255)
                                          aTAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCAGCCATTCAGATa                                                                                                        >  1:3029794/1‑150 (MQ=255)
                                             aaGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCt                                                                                                     <  1:3699780/150‑1 (MQ=255)
                                             aaGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAAAGCGTATTTTTTCCGCCATTCAGATAGCt                                                                                                     >  2:3581105/1‑150 (MQ=255)
                                              aGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTg                                                                                                    >  2:205285/1‑150 (MQ=255)
                                              aGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTg                                                                                                    <  2:869127/150‑1 (MQ=255)
                                              aGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCATATTTTTTCCGCCATTCAGATAGCTg                                                                                                    >  1:1652029/1‑150 (MQ=255)
                                                tAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGaa                                                                                                  >  2:2676445/1‑150 (MQ=255)
                                                 aaTGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGaaa                                                                                                 >  2:2523414/1‑150 (MQ=255)
                                                     aCATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAAtgct                                                                                             >  1:3569346/1‑150 (MQ=255)
                                                        tctcTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCg                                                                                          >  2:23508/1‑150 (MQ=255)
                                                          tctTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGa                                                                                        <  1:3090623/150‑1 (MQ=255)
                                                              tttCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTa                                                                                    >  1:125887/1‑150 (MQ=255)
                                                               ttCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTaa                                                                                   <  1:3097815/150‑1 (MQ=255)
                                                                  gTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATg                                                                                >  2:2476223/1‑150 (MQ=255)
                                                                  gTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATg                                                                                <  1:1742576/150‑1 (MQ=255)
                                                                    ttctttcAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGaa                                                                              >  1:2108472/1‑150 (MQ=255)
                                                                    ttctttcAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGaa                                                                              <  1:440502/150‑1 (MQ=255)
                                                                      ctttcAAAATCCTAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACTAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAaga                                                                            <  2:174779/150‑1 (MQ=255)
                                                                       tttcAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAagag                                                                           <  1:1112118/150‑1 (MQ=255)
                                                                         tcAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTg                                                                         <  2:1451222/150‑1 (MQ=255)
                                                                          cAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGc                                                                        >  1:3783163/1‑150 (MQ=255)
                                                                           aaaaTCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCt                                                                       >  1:3359162/1‑150 (MQ=255)
                                                                              aTCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAAt                                                                    <  1:2916752/150‑1 (MQ=255)
                                                                                 aTAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGcc                                                                 >  2:838953/1‑150 (MQ=255)
                                                                                    gAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCgg                                                              >  1:2386500/1‑150 (MQ=255)
                                                                                    gAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCgg                                                              >  1:480198/1‑150 (MQ=255)
                                                                                     aaaCGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGt                                                             <  2:1253315/150‑1 (MQ=255)
                                                                                       aCGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTaa                                                           <  1:2892589/150‑1 (MQ=255)
                                                                                          gTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAAttt                                                        >  2:1607686/1‑150 (MQ=255)
                                                                                            aGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTc                                                      >  1:3264558/1‑150 (MQ=255)
                                                                                             gtgtCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCt                                                     >  1:554798/1‑150 (MQ=255)
                                                                                             gtgtCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCt                                                     >  1:415334/1‑150 (MQ=255)
                                                                                             gtgtCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCt                                                     <  2:137470/150‑1 (MQ=255)
                                                                                             gtgtCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCt                                                     <  2:3403921/150‑1 (MQ=255)
                                                                                              tgtCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCta                                                    >  2:1932902/1‑150 (MQ=255)
                                                                                                 ccTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATTTCGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATaa                                                 <  1:1468080/150‑1 (MQ=255)
                                                                                                 ccTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATaa                                                 >  1:3034531/1‑150 (MQ=255)
                                                                                                      gTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGa                                            >  1:3644702/1‑150 (MQ=255)
                                                                                                          tGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGa                                        >  2:573674/1‑150 (MQ=255)
                                                                                                          tGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGa                                        >  1:1365175/1‑150 (MQ=255)
                                                                                                          tGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGa                                        <  1:3661492/150‑1 (MQ=255)
                                                                                                           gACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAg                                       <  1:2629110/150‑1 (MQ=255)
                                                                                                           gACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAg                                       <  1:3228983/150‑1 (MQ=255)
                                                                                                              cTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCtt                                    >  1:1669425/1‑150 (MQ=255)
                                                                                                              cTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCtt                                    >  2:444774/1‑150 (MQ=255)
                                                                                                              cTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCtt                                    <  2:2187321/150‑1 (MQ=255)
                                                                                                              cTGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCtt                                    >  1:3562820/1‑150 (MQ=255)
                                                                                                               tGGAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTc                                   >  1:3486052/1‑150 (MQ=255)
                                                                                                                 gAATAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCAt                                 <  2:2547010/150‑1 (MQ=255)
                                                                                                                  aaTAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCAtc                                >  2:1972427/1‑150 (MQ=255)
                                                                                                                  aaTAGAATTATTGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCAtc                                >  1:831046/1‑150 (MQ=255)
                                                                                                                            ttGACGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGtt                      <  2:2395762/150‑1 (MQ=255)
                                                                                                                                cGATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAAt                  <  2:1552871/150‑1 (MQ=255)
                                                                                                                                 gATGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAAtt                 <  1:589763/150‑1 (MQ=255)
                                                                                                                                  aTGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAAttt                <  2:1275537/150‑1 (MQ=255)
                                                                                                                                   tGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTa               <  2:319681/150‑1 (MQ=255)
                                                                                                                                   tGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTa               <  2:291217/150‑1 (MQ=255)
                                                                                                                                   tGAATTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTa               <  2:415334/150‑1 (MQ=255)
                                                                                                                                      aTTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACt            >  2:2149843/1‑150 (MQ=255)
                                                                                                                                      aTTTGCAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACt            >  1:751155/1‑150 (MQ=255)
                                                                                                                                           cAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCa       <  1:2387686/150‑1 (MQ=255)
                                                                                                                                           cAGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCa       <  1:3179988/150‑1 (MQ=255)
                                                                                                                                            aGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCAt      <  2:3137020/150‑1 (MQ=255)
                                                                                                                                            aGACACTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCAt      >  2:2660475/1‑150 (MQ=255)
                                                                                                                                               cacTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCt   <  1:2852824/150‑1 (MQ=255)
                                                                                                                                               cacTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCt   <  1:1534955/150‑1 (MQ=255)
                                                                                                                                               cacTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCt   <  2:1679028/150‑1 (MQ=255)
                                                                                                                                               cacTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCt   >  1:2196570/1‑150 (MQ=255)
                                                                                                                                                acTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCtt  <  2:3630948/150‑1 (MQ=255)
                                                                                                                                                acTTATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCtt  <  1:2482574/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCTT  >  CP009273/4567131‑4567424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑