BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	350,559	G→T	intergenic (+256/‑74)	prpE → / → codB	propionate‑‑CoA ligase/cytosine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	350,559	0	G	T	100.0%	746.5 / NA	223	intergenic (+256/‑74)	prpE/codB	propionate‑‑CoA ligase/cytosine transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (99/124); total (99/124)

Only 100 of 223 total aligned reads displayed.

GCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGT  >  CP009273/350412‑350705

gcgcTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTAt                                                                                                                                                  <  2:411725/150‑1 (MQ=255)

gcgcTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTAt                                                                                                                                                  <  2:1192410/150‑1 (MQ=255)

   cTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGc                                                                                                                                               >  2:702999/1‑150 (MQ=255)

        gTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCttttt                                                                                                                                          <  1:3072244/150‑1 (MQ=255)

        gTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCttttt                                                                                                                                          <  1:2298176/150‑1 (MQ=255)

        gTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCttttt                                                                                                                                          >  1:3351604/1‑150 (MQ=255)

         tCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTa                                                                                                                                         <  2:3478890/150‑1 (MQ=255)

          cGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTAt                                                                                                                                        >  2:156954/1‑150 (MQ=255)

           gCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATc                                                                                                                                       >  2:1409887/1‑150 (MQ=255)

           gCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTAATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACATTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATc                                                                                                                                       >  2:3307462/1‑150 (MQ=255)

            cATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCt                                                                                                                                      >  2:223238/1‑150 (MQ=255)

              tCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTc                                                                                                                                    <  2:1283162/150‑1 (MQ=255)

              tCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTc                                                                                                                                    >  2:1942653/1‑150 (MQ=255)

              tCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTc                                                                                                                                    <  1:2087212/150‑1 (MQ=255)

               ccGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCa                                                                                                                                   <  1:1886937/150‑1 (MQ=255)

                cGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAg                                                                                                                                  >  2:2260828/1‑150 (MQ=255)

                  acacCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGAt                                                                                                                                <  2:3671648/150‑1 (MQ=255)

                  acacCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGAt                                                                                                                                <  1:1073200/150‑1 (MQ=255)

                   cacCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATg                                                                                                                               >  2:3552658/1‑150 (MQ=255)

                            cAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATg                                                                                                                      >  1:1751869/1‑150 (MQ=255)

                            cAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATg                                                                                                                      >  1:1751791/1‑150 (MQ=255)

                               aTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATgcgg                                                                                                                   >  1:2339161/1‑150 (MQ=255)

                                 gCCTGATGTGACGCTGACGCGTATTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATgcggcg                                                                                                                 <  2:2929004/150‑1 (MQ=255)

                                 gCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAAATATTGCTTTTTATCTTCAGATGAATAGAATgcggcg                                                                                                                 <  1:2554705/150‑1 (MQ=255)

                                  ccTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGcggcgg                                                                                                                >  2:735135/1‑150 (MQ=255)

                                    tGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAt                                                                                                              <  2:2023122/150‑1 (MQ=255)

                                           aCGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTggg                                                                                                       <  1:80811/150‑1 (MQ=255)

                                             gCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGt                                                                                                     <  1:1285527/150‑1 (MQ=255)

                                             gCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGt                                                                                                     >  2:2193332/1‑150 (MQ=255)

                                             gCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGt                                                                                                     <  1:2471095/150‑1 (MQ=255)

                                              cTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGtt                                                                                                    <  2:1207201/150‑1 (MQ=255)

                                               tGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGttt                                                                                                   <  1:2657912/150‑1 (MQ=255)

                                               tGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGttt                                                                                                   <  1:236827/150‑1 (MQ=255)

                                               tGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGttt                                                                                                   <  1:2517928/150‑1 (MQ=255)

                                                gACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTc                                                                                                  >  1:3676595/1‑150 (MQ=255)

                                                    cgTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAAc                                                                                              <  2:3042798/150‑1 (MQ=255)

                                                    cgTCTTATCAGGCCTACACACTGTTTTTACACCGATAATTTTTCCCCTTCCTTTTTGCTCTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCaaaa                                                                                              <  1:94849/150‑2 (MQ=255)

                                                     gTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACa                                                                                             >  2:1610324/1‑150 (MQ=255)

                                                     gTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACa                                                                                             <  1:35391/150‑1 (MQ=255)

                                                       cTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGc                                                                                           >  1:650384/1‑150 (MQ=255)

                                                        ttATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCa                                                                                          >  2:467544/1‑150 (MQ=255)

                                                        ttATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAAAAGCa                                                                                          >  2:3005510/1‑150 (MQ=255)

                                                         tATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCaa                                                                                         <  2:2382526/150‑1 (MQ=255)

                                                          aTCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCaaa                                                                                        >  1:1510803/1‑150 (MQ=255)

                                                            cAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCaaaaa                                                                                      >  2:1065789/1‑150 (MQ=255)

                                                             aGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATTGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAg                                                                                     <  1:576608/150‑1 (MQ=255)

                                                             aGGCCTACCCACTGTTTTTACACCGATAATTTGTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAg                                                                                     <  2:1400534/150‑1 (MQ=255)

                                                              ggCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAgg                                                                                    <  2:3171510/150‑1 (MQ=255)

                                                                  tACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGa                                                                                >  1:762939/1‑150 (MQ=255)

                                                                   aCCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGaa                                                                               <  1:2896390/150‑1 (MQ=255)

                                                                    cccACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAAt                                                                              <  2:1751791/150‑1 (MQ=255)

                                                                    cccACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAAt                                                                              <  2:2519558/150‑1 (MQ=255)

                                                                    cccACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAAt                                                                              <  2:1751869/150‑1 (MQ=255)

                                                                      cACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAAttt                                                                            >  1:2495605/1‑150 (MQ=255)

                                                                       aCTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTc                                                                           <  1:347039/150‑1 (MQ=255)

                                                                       aCTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTc                                                                           <  2:2462534/150‑1 (MQ=255)

                                                                       aCTGTTTTTACACCGATAATTTTACCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTc                                                                           <  1:1613606/150‑1 (MQ=255)

                                                                         tGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCgt                                                                         >  1:2780896/1‑150 (MQ=255)

                                                                          gTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCgtg                                                                        <  1:3270438/150‑1 (MQ=255)

                                                                          gTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCgtg                                                                        <  1:2391062/150‑1 (MQ=255)

                                                                           tttttACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCgtgt                                                                       <  1:399937/150‑1 (MQ=255)

                                                                            ttttACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCCCGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTc                                                                      <  2:2874898/150‑1 (MQ=255)

                                                                                acacCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCaa                                                                  >  2:3605484/1‑150 (MQ=255)

                                                                                 cacCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAg                                                                 <  1:5096/150‑1 (MQ=255)

                                                                                 cacCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAg                                                                 >  1:2081536/1‑150 (MQ=255)

                                                                                    cGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGACTTTCGTGTCGCAAGATa                                                              >  2:1833886/1‑150 (MQ=255)

                                                                                        aaTTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATaacaa                                                          <  1:1826935/150‑1 (MQ=255)

                                                                                         aTTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATaacaac                                                         >  2:1133443/1‑150 (MQ=255)

                                                                                           ttttCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACtt                                                       <  1:1579172/150‑1 (MQ=255)

                                                                                              tCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAg                                                    >  2:3765033/1‑150 (MQ=255)

                                                                                               cccccACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGc                                                   <  2:3562877/150‑1 (MQ=255)

                                                                                                   cACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAgg                                               >  1:3384429/1‑150 (MQ=255)

                                                                                                     ccTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAgggg                                             <  1:2857641/150‑1 (MQ=255)

                                                                                                     ccTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAgggg                                             <  1:1742266/150‑1 (MQ=255)

                                                                                                       ttttttCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGcc                                           <  1:3184240/150‑1 (MQ=255)

                                                                                                       tttttGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGcc                                           <  2:781921/150‑1 (MQ=255)

                                                                                                          ttGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGt                                        >  2:3418730/1‑150 (MQ=255)

                                                                                                              aCTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCg                                    >  1:3202649/1‑150 (MQ=255)

                                                                                                              aCTCATTCATATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCg                                    >  1:1302250/1‑150 (MQ=255)

                                                                                                                tcattcatATAAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCa                                  >  2:3715006/1‑150 (MQ=255)

                                                                                                                         taAAAAATATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGcgcg                         <  2:650384/150‑1 (MQ=255)

                                                                                                                          aaaaaaTATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCgg                        <  1:2143019/150‑1 (MQ=255)

                                                                                                                              aaTATATTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAg                    <  2:2860957/150‑1 (MQ=255)

                                                                                                                               atatatTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAACAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAgg                   <  2:1817546/150‑1 (MQ=255)

                                                                                                                               atatatTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAgg                   <  1:426295/150‑1 (MQ=255)

                                                                                                                                 atatTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAgggg                 <  1:3787718/150‑1 (MQ=255)

                                                                                                                                   atTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTa               <  2:3260006/150‑1 (MQ=255)

                                                                                                                                   atTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTa               >  2:2290042/1‑150 (MQ=255)

                                                                                                                                   atTTCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTa               <  2:2780896/150‑1 (MQ=255)

                                                                                                                                     ttCCCCACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTAtt             <  1:156954/150‑1 (MQ=255)

                                                                                                                                      tCCCCACGAAAACTATAGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTAGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGTGGTATTg            >  1:2449711/1‑150 (MQ=255)

                                                                                                                                       ccccACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTgg           <  1:1409887/150‑1 (MQ=255)

                                                                                                                                        cccACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCACAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGc          >  1:3053176/1‑150 (MQ=255)

                                                                                                                                         ccACGAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCa         >  2:2117814/1‑150 (MQ=255)

                                                                                                                                             gAAAACTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGa     >  1:2165666/1‑150 (MQ=255)

                                                                                                                                              aaaaCTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGAc    <  2:476550/150‑1 (MQ=255)

                                                                                                                                              aaaaCTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGAc    >  1:857260/1‑150 (MQ=255)

                                                                                                                                               aaaCTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACg   >  1:504471/1‑150 (MQ=255)

                                                                                                                                               aaaCTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACg   <  1:1465416/150‑1 (MQ=255)

                                                                                                                                                aaCTATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGt  >  2:2169386/1‑150 (MQ=255)

GCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGT  >  CP009273/350412‑350705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`