BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,596,243	T→A	intergenic (‑25/+243)	leuQ ← / ← rsmC	tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,596,243	0	T	A	100.0%	809.8 / NA	241	intergenic (‑25/+243)	leuQ/rsmC	tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (136/105); total (136/105)

Only 100 of 241 total aligned reads displayed.

CCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGC  >  CP009273/4596097‑4596388

ccTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCt                                                                                                                                                >  2:1048552/1‑150 (MQ=255)

  ttCGCCCAGTGCGATCAATATCAACGTGGTTTTTGTTGCGTGGGGGGGGACTTGAACCCCCACGTACGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTAc                                                                                                                                              <  1:2260110/150‑1 (MQ=21)

   tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTAcc                                                                                                                                             >  1:3737818/1‑150 (MQ=255)

     gCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCAc                                                                                                                                           <  2:515487/150‑1 (MQ=255)

         aGTTCGATCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATACCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAg                                                                                                                                       <  1:1243842/150‑1 (MQ=255)

         aGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAg                                                                                                                                       <  1:3141885/150‑1 (MQ=255)

         aGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAg                                                                                                                                       >  1:2447256/1‑150 (MQ=255)

          gTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAgg                                                                                                                                      <  2:2390322/150‑1 (MQ=255)

            gCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGgcg                                                                                                                                    <  2:660370/150‑1 (MQ=255)

               aGCAATATCAACGTGGTGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGACTAGCGACCACGGAGGCGCAt                                                                                                                                 >  2:2149018/1‑150 (MQ=255)

                  aaTATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCt                                                                                                                              >  2:2937865/1‑150 (MQ=255)

                  aaTATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCt                                                                                                                              >  1:507670/1‑150 (MQ=255)

                   atatCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTa                                                                                                                             <  2:3611289/150‑1 (MQ=255)

                     atCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGt                                                                                                                           >  2:1540761/1‑150 (MQ=255)

                      tCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTg                                                                                                                          >  2:3406400/1‑150 (MQ=255)

                         aCGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGtt                                                                                                                       >  2:3116308/1‑150 (MQ=255)

                         aCGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGttt                                                                                                                      >  1:2163038/1‑150 (MQ=255)

                           gTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGtttt                                                                                                                     <  2:3200679/150‑1 (MQ=255)

                             ggTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCa                                                                                                                   <  1:27776/150‑1 (MQ=255)

                                tttgGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCt                                                                                                                <  2:236147/150‑1 (MQ=255)

                                       gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTc                                                                                                         <  2:2678621/150‑1 (MQ=255)

                                        cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCa                                                                                                        <  2:3049240/150‑1 (MQ=255)

                                            gggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTc                                                                                                                               <  1:557957/123‑1 (MQ=255)

                                            gggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTc                                                                                                                               >  2:557957/1‑123 (MQ=255)

                                               ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTa                                                                                                 <  2:433433/150‑1 (MQ=255)

                                                 ggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAAt                                                                                               >  2:2592135/1‑150 (MQ=255)

                                                     ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATc                                                                                           >  1:1401703/1‑150 (MQ=255)

                                                      tGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCg                                                                                          >  2:721891/1‑150 (MQ=255)

                                                      tGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCg                                                                                          <  1:1725572/150‑1 (MQ=255)

                                                        aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTCTTCAGCTTTTCGTCAATAGTTAATTATCGAc                                                                                        >  1:1582640/1‑150 (MQ=255)

                                                         aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACa                                                                                       >  2:2525863/1‑150 (MQ=255)

                                                          cccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACag                                                                                      <  1:3246690/150‑1 (MQ=255)

                                                           ccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACaga                                                                                     >  2:2788274/1‑150 (MQ=255)

                                                            cccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACagag                                                                                    >  2:138342/1‑150 (MQ=255)

                                                               aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGgtg                                                                                 <  1:194482/150‑1 (MQ=255)

                                                                 gTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCTCGGAGGCGAATTCTAGTGTTTTTCAGCTTTTCGTCAATATTTAATTATCGACAGAGGTGTa                                                                               <  2:3676769/150‑1 (MQ=37)

                                                                  tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTaa                                                                              >  1:1002118/1‑150 (MQ=255)

                                                                     gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTg                                                                           >  2:1951820/1‑150 (MQ=255)

                                                                     gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTg                                                                           >  2:200260/1‑150 (MQ=255)

                                                                      tAAGGACACTAACACCTGACGCTAGCGCGTCTACCAAGTCCGCCACCTTCGCATACCATCAATTCTGAAAAAGAATAGCTACCACGGAGGCGCAGTCTAGTGGTTTTCTGCGGTGCGTCCATAGTTCCTTATCGCCAGCGGGGTACTTGc                                                                          >  2:3625640/1‑150 (MQ=21)

                                                                      tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGc                                                                          >  2:1730584/1‑150 (MQ=255)

                                                                       aaGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCt                                                                         >  2:81476/1‑150 (MQ=255)

                                                                       aaGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCt                                                                         <  1:2664450/150‑1 (MQ=255)

                                                                           acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGaa                                                                     <  1:2046343/150‑1 (MQ=255)

                                                                           acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGaa                                                                     <  1:1598273/150‑1 (MQ=255)

                                                                              cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGaaaaa                                                                  <  2:2191850/150‑1 (MQ=255)

                                                                               tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAAt                                                                 >  1:1126360/1‑150 (MQ=255)

                                                                               tAACACCTGAAGCTAGCGCGTCTACCAAGTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAAt                                                                 <  2:243530/150‑1 (MQ=255)

                                                                                aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATg                                                                <  2:1462848/150‑1 (MQ=255)

                                                                                 acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGt                                                               <  2:2587430/150‑1 (MQ=255)

                                                                                  cacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTc                                                              <  1:760588/150‑1 (MQ=255)

                                                                                   acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTcc                                                             <  2:3263167/150‑1 (MQ=255)

                                                                                   acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTcc                                                             <  2:1365612/150‑1 (MQ=255)

                                                                                    ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCa                                                            <  1:604532/150‑1 (MQ=255)

                                                                                     cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCAt                                                           <  2:2497330/150‑1 (MQ=255)

                                                                                     cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCAt                                                           <  2:2070125/150‑1 (MQ=255)

                                                                                     cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCAt                                                           <  1:2159492/150‑1 (MQ=255)

                                                                                       gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGGGGTGTAATTGCTGGAAAAATGTCCATCa                                                         >  1:1874631/1‑150 (MQ=255)

                                                                                         aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAgg                                                       >  2:1100215/1‑150 (MQ=255)

                                                                                           cTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGaa                                                     >  1:3429234/1‑150 (MQ=255)

                                                                                            tAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGaaa                                                    >  1:37942/1‑150 (MQ=255)

                                                                                            tAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGaaa                                                    >  1:1548071/1‑150 (MQ=255)

                                                                                             aGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAAc                                                   <  1:2626323/150‑1 (MQ=255)

                                                                                             aGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAAc                                                   <  1:2834845/150‑1 (MQ=255)

                                                                                                gcgTCTACCAAGTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAg                                                <  2:2847335/150‑1 (MQ=255)

                                                                                                 cgTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGc                                               <  1:3088920/150‑1 (MQ=255)

                                                                                                 cgTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGc                                               <  1:3272652/150‑1 (MQ=255)

                                                                                                  gTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAACCTAGCg                                              >  2:3520175/1‑150 (MQ=255)

                                                                                                    cTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTg                                            <  1:140786/150‑1 (MQ=255)

                                                                                                    cTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTg                                            >  1:2001775/1‑150 (MQ=255)

                                                                                                         aaTTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGt                                       >  2:1635947/1‑150 (MQ=255)

                                                                                                          aTTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGtt                                      >  2:2304569/1‑150 (MQ=255)

                                                                                                          aTTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGtt                                      >  1:165606/1‑150 (MQ=255)

                                                                                                          aTTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGtt                                      <  1:1738008/150‑1 (MQ=255)

                                                                                                           ttCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGttt                                     <  1:2404396/150‑1 (MQ=255)

                                                                                                           ttCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGttt                                     <  1:2369775/150‑1 (MQ=255)

                                                                                                           ttCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGttt                                     <  1:2315230/150‑1 (MQ=255)

                                                                                                              cGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGt                                  <  2:510717/150‑1 (MQ=255)

                                                                                                              cGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGt                                  >  2:1658871/1‑150 (MQ=255)

                                                                                                                 cACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTatg                               >  2:536609/1‑150 (MQ=255)

                                                                                                                  aCCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTatgc                              >  2:1782786/1‑150 (MQ=255)

                                                                                                                    cTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTatgcat                            <  1:584022/150‑1 (MQ=255)

                                                                                                                          aTACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCgggg                      >  2:597532/1‑150 (MQ=255)

                                                                                                                           tACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGttt                                     >  1:304224/1‑134 (MQ=255)

                                                                                                                           tACCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCggggg                     >  2:3426199/1‑150 (MQ=255)

                                                                                                                            aCCATCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGc                    >  1:3520253/1‑150 (MQ=255)

                                                                                                                                tCAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagat                >  1:664217/1‑150 (MQ=255)

                                                                                                                                 cAATTCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatg               <  1:1048552/150‑1 (MQ=255)

                                                                                                                                    ttCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatgcca            <  2:2087255/150‑1 (MQ=255)

                                                                                                                                    ttCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatgcca            >  1:1196659/1‑150 (MQ=255)

                                                                                                                                     tCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatgccag           <  1:2687785/150‑1 (MQ=255)

                                                                                                                                     tCTTAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatgccag           <  1:783209/150‑1 (MQ=255)

                                                                                                                                       ttAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatgccagat         <  2:1852150/150‑1 (MQ=255)

                                                                                                                                        tAAAAAGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGG‑AAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatgccagatgc       <  1:1827224/150‑1 (MQ=255)

                                                                                                                                         aaaaaGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGcagatgccagatgc       <  1:3683584/150‑1 (MQ=255)

                                                                                                                                          aaaaGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCg      >  2:3019635/1‑150 (MQ=255)

                                                                                                                                          aaaaGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCg      <  1:3186728/150‑1 (MQ=255)

                                                                                                                                          aaaaGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCg      >  1:3500532/1‑150 (MQ=255)

                                                                                                                                            aaGAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGAc    >  2:2386125/1‑150 (MQ=255)

                                                                                                                                              gAATAGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGc  >  1:956161/1‑150 (MQ=255)

CCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAATAGTTAATTATCGACAGAGGTGTAATTGCTGGAAAAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGC  >  CP009273/4596097‑4596388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`