Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,600,972 G→T intergenic (+152/‑241) prfC → / → osmY peptide chain release factor RF‑3/periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,600,9720GT100.0% 610.1 / NA 183intergenic (+152/‑241)prfC/osmYpeptide chain release factor RF‑3/periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (94/89);  total (94/89)

Only 100 of 186 total aligned reads displayed.

TGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTGATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCTTTTGTGATGGCGACCA  >  CP009273/4600825‑4601118
                                                                                                                                                   |                                                                                                                                                  
   cTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGAc                                                                                                                                               <  2:1116695/150‑1 (MQ=255)
     ccAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACAt                                                                                                                                             >  1:1634361/1‑150 (MQ=255)
      cAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACAtt                                                                                                                                            <  2:1207536/150‑1 (MQ=255)
       aGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCTCTTATATCCCGAGCTGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACAttt                                                                                                                                           >  1:3650332/1‑150 (MQ=255)
       aGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACAttt                                                                                                                                           <  1:1977752/150‑1 (MQ=255)
       aGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTGATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACACAGTTATGACAttt                                                                                                                                           >  2:2483371/1‑150 (MQ=255)
        gggCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTc                                                                                                                                          >  2:1615583/1‑150 (MQ=255)
          gcgcGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTg                                                                                                                                        <  1:2950567/150‑1 (MQ=255)
             cgGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACg                                                                                                                                     <  1:3255147/150‑1 (MQ=255)
                 aGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGt                                                                                                                                 >  1:2135607/1‑150 (MQ=255)
                 aGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGt                                                                                                                                 >  1:2893522/1‑150 (MQ=255)
                   ccGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTa                                                                                                                               <  2:1031015/150‑1 (MQ=255)
                     gCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACTGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTaaa                                                                                                                             <  2:450589/150‑1 (MQ=255)
                     gCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTaaa                                                                                                                             <  1:881626/150‑1 (MQ=255)
                      cTGCGCCCTGACAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAAt                                                                                                                            <  1:2633482/150‑1 (MQ=255)
                       tGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATa                                                                                                                           <  1:1175513/150‑1 (MQ=255)
                        gcgcCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATAc                                                                                                                          <  2:2653837/150‑1 (MQ=255)
                           cccTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGt                                                                                                                       >  1:3033159/1‑150 (MQ=255)
                           cccTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGt                                                                                                                       >  1:1132333/1‑150 (MQ=255)
                              tGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCa                                                                                                                    <  2:708583/150‑1 (MQ=255)
                                   aTTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCg                                                                                                               >  1:1472203/1‑150 (MQ=255)
                                   aTTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCg                                                                                                               >  1:2282117/1‑150 (MQ=255)
                                   aTTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCg                                                                                                               >  1:2282201/1‑150 (MQ=255)
                                         cttccttATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGata                                                                                                         <  1:1142719/150‑1 (MQ=255)
                                         cttccttATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGata                                                                                                         <  1:2444271/150‑1 (MQ=255)
                                           tccttATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATc                                                                                                       >  1:790755/1‑150 (MQ=255)
                                            ccttATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCa                                                                                                      <  1:3300434/150‑1 (MQ=255)
                                             cttATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAg                                                                                                     >  2:2132355/1‑150 (MQ=255)
                                             cttATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAg                                                                                                     >  1:917394/1‑150 (MQ=255)
                                                  taGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCt                                                                                                <  1:2301825/150‑1 (MQ=255)
                                                    gCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTaa                                                                                              <  2:3330154/150‑1 (MQ=255)
                                                     ccGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTaaa                                                                                             >  2:3485501/1‑150 (MQ=255)
                                                         ttACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCg                                                                                         >  2:264619/1‑150 (MQ=255)
                                                          tACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGt                                                                                        <  1:405729/150‑1 (MQ=255)
                                                           aCGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTc                                                                                       <  1:1500537/150‑1 (MQ=255)
                                                            cGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCg                                                                                      <  2:77402/150‑1 (MQ=255)
                                                              gAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGAt                                                                                    >  1:563033/1‑150 (MQ=255)
                                                              gAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGAt                                                                                    <  1:33627/150‑1 (MQ=255)
                                                              gAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGAt                                                                                    >  1:2218967/1‑150 (MQ=255)
                                                              gAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGAt                                                                                    <  1:33475/150‑1 (MQ=255)
                                                              gAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGAt                                                                                    <  1:3176496/150‑1 (MQ=255)
                                                              gAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGAt                                                                                    <  2:3318820/150‑1 (MQ=255)
                                                              gAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGAt                                                                                    <  2:534203/150‑1 (MQ=255)
                                                                 tGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGt                                                                                 <  2:2109282/150‑1 (MQ=255)
                                                                 tGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGt                                                                                 <  1:2500148/150‑1 (MQ=255)
                                                                   ttCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCa                                                                               >  1:666329/1‑150 (MQ=255)
                                                                     cTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCAtt                                                                             >  1:854248/1‑150 (MQ=255)
                                                                      ttAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTc                                                                            >  1:1462852/1‑150 (MQ=255)
                                                                       tAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCt                                                                           >  2:1985278/1‑150 (MQ=255)
                                                                       tAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCt                                                                           <  2:2068435/150‑1 (MQ=255)
                                                                        aaaaCATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTTTCATTCTa                                                                          >  2:47999/1‑150 (MQ=255)
                                                                          aaCATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTAcc                                                                        <  2:3033159/150‑1 (MQ=255)
                                                                            cATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGa                                                                      <  1:2161027/150‑1 (MQ=255)
                                                                             aTTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGat                                                                     <  2:3699901/150‑1 (MQ=255)
                                                                              ttCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGata                                                                    >  2:2352930/1‑150 (MQ=255)
                                                                                 aCTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATta                                                                 >  2:1140078/1‑150 (MQ=255)
                                                                                  cTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATtaa                                                                <  2:2804102/150‑1 (MQ=255)
                                                                                       gCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACt                                                           >  1:3409408/1‑150 (MQ=255)
                                                                                        cTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTg                                                          >  2:939720/1‑150 (MQ=255)
                                                                                        cTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTg                                                          <  2:2506966/150‑1 (MQ=255)
                                                                                         ttATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGa                                                         <  1:1592329/150‑1 (MQ=255)
                                                                                          tATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCACTCTACCGATATTAATAACTGAt                                                        >  2:2703951/1‑150 (MQ=255)
                                                                                             gTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCa                                                     <  1:1709722/150‑1 (MQ=255)
                                                                                               tttCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCaga                                                   >  1:1934786/1‑150 (MQ=255)
                                                                                                  cGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGc                                                <  2:1882414/150‑1 (MQ=255)
                                                                                                  cGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGc                                                <  2:1882440/150‑1 (MQ=255)
                                                                                                  cGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGc                                                >  1:3157860/1‑150 (MQ=255)
                                                                                                   gCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCt                                               <  2:1634361/150‑1 (MQ=255)
                                                                                                         atCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATg                                         <  1:3782106/150‑1 (MQ=255)
                                                                                                         atCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATg                                         >  1:987346/1‑150 (MQ=255)
                                                                                                          tCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATgg                                        <  1:2876823/150‑1 (MQ=255)
                                                                                                            ccGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTc                                      <  1:1308852/150‑1 (MQ=255)
                                                                                                            ccGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTc                                      <  2:1953091/150‑1 (MQ=255)
                                                                                                                 cGGTTTCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTAt                                 <  2:3401907/150‑1 (MQ=255)
                                                                                                                    tttCAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATtca                              >  1:1251322/1‑150 (MQ=255)
                                                                                                                       cAAAATTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATtcatca                           <  2:3650332/150‑1 (MQ=255)
                                                                                                                        aaaaTTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCAc                          >  2:2783155/1‑150 (MQ=255)
                                                                                                                         aaaTTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACt                         <  2:2289678/150‑1 (MQ=255)
                                                                                                                         aaaTTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACt                         >  1:2839938/1‑150 (MQ=255)
                                                                                                                         aaaTTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACt                         <  1:1862295/150‑1 (MQ=255)
                                                                                                                          aaTTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTc                        <  2:3245214/150‑1 (MQ=255)
                                                                                                                           aTTGTGATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCa                       >  2:1552832/1‑150 (MQ=255)
                                                                                                                             tgtgATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATc                     >  2:19978/1‑150 (MQ=255)
                                                                                                                              gtgATCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCg                    <  1:1568795/150‑1 (MQ=255)
                                                                                                                                 aTCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCtt                 >  2:113432/1‑150 (MQ=255)
                                                                                                                                 aTCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCtt                 >  2:3645490/1‑150 (MQ=255)
                                                                                                                                 aTCTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCtt                 >  2:1751401/1‑150 (MQ=255)
                                                                                                                                   cTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCtttt               <  2:2893522/150‑1 (MQ=255)
                                                                                                                                   cTATATTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCtttt               >  1:3690667/1‑150 (MQ=255)
                                                                                                                                    tataTTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATTAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTTATGGTCGTTATTCATCACTCATCGCTTTtg              <  1:2180905/150‑1 (MQ=255)
                                                                                                                                    tataTTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCTTTtg              >  2:158956/1‑150 (MQ=255)
                                                                                                                                    tataTTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCTTTtg              <  2:1467698/150‑1 (MQ=255)
                                                                                                                                     atatTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCTTTtgt             >  1:1873612/1‑150 (MQ=255)
                                                                                                                                     atatTTAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCTTTtgt             >  1:2761349/1‑150 (MQ=255)
                                                                                                                                         ttAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCTTTTGTGATg         <  2:1963320/150‑1 (MQ=255)
                                                                                                                                          tAACAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCTTTTGTGATgg        >  2:363230/1‑150 (MQ=255)
                                                                                                                                             cAAAGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCTTTTGTGATGGCGa     >  1:2748345/1‑150 (MQ=255)
                                                                                                                                              aaaGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCTTTTGTGATGGCGAc    >  2:2329756/1‑150 (MQ=255)
                                                                                                                                               aaGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCTTTTGTGATGGCGAcc   <  1:104503/150‑1 (MQ=255)
                                                                                                                                                aGTTATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCTTTTGTGATGGCGACCa  >  1:2774139/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAACATTCACTTTTGCTTATGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTGATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAGGCTGTAATGGTCGTTATTCATCACTCATCGCTTTTGTGATGGCGACCA  >  CP009273/4600825‑4601118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑