BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,609,543	G→T	E42* (GAA→TAA)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,609,543	0	G	T	99.7%	955.7 / NA	286	E42* (GAA→TAA)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (164/121); minor base A (1/0); total (165/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

TGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCAC  >  CP009273/4609396‑4609689

tGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTaa                                                                                                                                                  >  1:3022149/1‑150 (MQ=255)

  aaGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGc                                                                                                                                                >  1:2097693/1‑150 (MQ=255)

  aaGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGc                                                                                                                                                >  1:2857055/1‑150 (MQ=255)

  aaGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGc                                                                                                                                                >  1:461468/1‑150 (MQ=255)

  aaGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGc                                                                                                                                                >  1:688755/1‑150 (MQ=255)

  aaGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGc                                                                                                                                                >  2:1554517/1‑150 (MQ=255)

      aCTGAGTACGGAGAACATATGAAACCTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGAt                                                                                                                                            <  2:3413449/150‑1 (MQ=255)

       cTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATa                                                                                                                                           <  1:941183/150‑1 (MQ=255)

       cTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATa                                                                                                                                           <  1:2845847/150‑1 (MQ=255)

        tGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATaa                                                                                                                                          <  2:2732477/150‑1 (MQ=255)

         gAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAAc                                                                                                                                         >  1:2943543/1‑150 (MQ=255)

            tACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGt                                                                                                                                      <  2:2001910/150‑1 (MQ=255)

             aCGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTc                                                                                                                                     <  2:384419/150‑1 (MQ=255)

              cGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCg                                                                                                                                    >  2:780467/1‑150 (MQ=255)

                  gaACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTaaa                                                                                                                                >  1:1185157/1‑150 (MQ=255)

                   aaCATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAg                                                                                                                               <  1:845218/150‑1 (MQ=255)

                   aaCATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAg                                                                                                                               <  1:1385161/150‑1 (MQ=255)

                     cATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGc                                                                                                                             >  1:1081640/1‑150 (MQ=255)

                       tatGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGccc                                                                                                                           >  1:855877/1‑150 (MQ=255)

                       tatGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGccc                                                                                                                           <  2:3595739/150‑1 (MQ=255)

                            aaCGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCa                                                                                                                      >  2:870006/1‑150 (MQ=255)

                                tGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCt                                                                                                                  <  1:325829/150‑1 (MQ=255)

                                tGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCt                                                                                                                  <  2:1081481/150‑1 (MQ=255)

                                 gCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTg                                                                                                                 >  1:2442531/1‑150 (MQ=255)

                                 gCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTg                                                                                                                 >  1:91213/1‑150 (MQ=255)

                                  cATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGc                                                                                                                >  2:218213/1‑150 (MQ=255)

                                    tttATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCa                                                                                                              >  1:2070159/1‑150 (MQ=255)

                                       attatGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAAt                                                                                                           >  1:1953114/1‑150 (MQ=255)

                                       attatGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAAt                                                                                                           >  1:2534943/1‑150 (MQ=255)

                                                 tGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTc                                                                                                 <  2:3406613/150‑1 (MQ=255)

                                                    aCTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTgg                                                                                              >  2:3533638/1‑150 (MQ=255)

                                                     cTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTggg                                                                                             >  1:1540842/1‑150 (MQ=255)

                                                        aTTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCt                                                                                          <  1:3781701/150‑1 (MQ=255)

                                                        aTTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCt                                                                                          >  2:442157/1‑150 (MQ=255)

                                                         ttCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTg                                                                                         <  1:3312899/150‑1 (MQ=255)

                                                          tCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGACCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTgg                                                                                        >  1:1746570/1‑150 (MQ=255)

                                                           cGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGc                                                                                       >  1:431301/1‑150 (MQ=255)

                                                           cGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGc                                                                                       <  2:245754/150‑1 (MQ=255)

                                                             gCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGa                                                                                     >  2:1459599/1‑150 (MQ=255)

                                                               aTCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGaaa                                                                                   >  1:360675/1‑150 (MQ=255)

                                                               aTCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGaaa                                                                                   >  2:2790751/1‑150 (MQ=255)

                                                                tCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAg                                                                                  >  1:3729259/1‑150 (MQ=255)

                                                                tCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAg                                                                                  >  2:3787068/1‑150 (MQ=255)

                                                                     gcTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCacac                                                                             >  1:1461037/1‑150 (MQ=255)

                                                                      cTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACg                                                                            >  2:1872468/1‑150 (MQ=255)

                                                                      cTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACg                                                                            >  1:2306358/1‑150 (MQ=255)

                                                                         cAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAg                                                                         <  1:381286/150‑1 (MQ=255)

                                                                         cAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAg                                                                         >  1:1597455/1‑150 (MQ=255)

                                                                          agaagaTGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAgg                                                                        <  2:1912949/150‑1 (MQ=255)

                                                                              gaTGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCt                                                                    >  1:1428429/1‑150 (MQ=255)

                                                                              gaTGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCt                                                                    <  1:2778157/150‑1 (MQ=255)

                                                                                 gCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTAcc                                                                 <  2:3022149/150‑1 (MQ=255)

                                                                                     aaCGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGtt                                                             <  2:2714698/150‑1 (MQ=255)

                                                                                      aCGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGttt                                                            <  1:2929028/150‑1 (MQ=255)

                                                                                      aCGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGttt                                                            <  2:1423699/150‑1 (MQ=255)

                                                                                       cGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTCCCGGTTTc                                                           >  1:3193901/1‑150 (MQ=255)

                                                                                       cGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTc                                                           <  2:3082811/150‑1 (MQ=255)

                                                                                        gCTTTGGTGACGTGGGGGCTGACACCCTGGGTCATATCGATGAAGCTTGTGCAAAAGGCTAAGCTGATTACGGTCGTAAAGGCCGGCTCATTCTGCCAATTCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCa                                                          <  2:1693360/150‑1 (MQ=255)

                                                                                        gCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCa                                                          <  2:1185157/150‑1 (MQ=255)

                                                                                        gCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCa                                                          <  2:37358/150‑1 (MQ=255)

                                                                                         cTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCAt                                                         >  1:1647773/1‑150 (MQ=255)

                                                                                           ttGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTc                                                       <  1:3688679/150‑1 (MQ=255)

                                                                                             ggTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCcg                                                     <  1:1838554/150‑1 (MQ=255)

                                                                                               tGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCcggc                                                   <  2:1239798/150‑1 (MQ=255)

                                                                                                 aCGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCcggcgg                                                 >  2:937934/1‑150 (MQ=255)

                                                                                                   gTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGa                                               >  2:3204949/1‑150 (MQ=255)

                                                                                                     cGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAAt                                             <  1:2054352/150‑1 (MQ=255)

                                                                                                     cGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAAt                                             <  1:2236141/150‑1 (MQ=255)

                                                                                                       gggCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATgg                                           >  2:545263/1‑150 (MQ=255)

                                                                                                         gCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGAc                                         <  1:1986820/150‑1 (MQ=255)

                                                                                                          cTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACg                                        >  2:734481/1‑150 (MQ=255)

                                                                                                            gACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGc                                      >  1:2488481/1‑150 (MQ=255)

                                                                                                              cacCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCaa                                    <  2:2097693/150‑1 (MQ=255)

                                                                                                               acCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAAc                                   <  2:3692902/150‑1 (MQ=255)

                                                                                                               acCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAAc                                   >  1:3377200/1‑150 (MQ=255)

                                                                                                                cccTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACg                                  >  2:701984/1‑150 (MQ=255)

                                                                                                                 ccTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGc                                 >  2:2908284/1‑150 (MQ=255)

                                                                                                                  cTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCt                                <  1:1609988/150‑1 (MQ=255)

                                                                                                                     ggTCATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGaa                             >  1:3518772/1‑150 (MQ=255)

                                                                                                                        cATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGtt                          >  1:62537/1‑150 (MQ=255)

                                                                                                                        cATATCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGtt                          >  2:3345516/1‑150 (MQ=255)

                                                                                                                         atatCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTa                         <  1:2717615/150‑1 (MQ=255)

                                                                                                                         atatCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTa                         <  2:2319586/150‑1 (MQ=255)

                                                                                                                           atCGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATc                       <  2:3234488/150‑1 (MQ=255)

                                                                                                                             cGCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCgg                     >  2:2124298/1‑150 (MQ=255)

                                                                                                                              gCAGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGgc                    <  1:1004112/150‑1 (MQ=255)

                                                                                                                                aGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGgcgc                  >  2:2423838/1‑150 (MQ=255)

                                                                                                                                aGAAGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGATCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGgcgc                  >  1:2948653/1‑150 (MQ=255)

                                                                                                                                   aGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTa               <  1:1700474/150‑1 (MQ=255)

                                                                                                                                   aGCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTa               >  1:3667787/1‑150 (MQ=255)

                                                                                                                                    gCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTAc              <  2:2601918/150‑1 (MQ=255)

                                                                                                                                    gCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTAc              <  1:605130/150‑1 (MQ=255)

                                                                                                                                    gCTTGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTAc              >  2:1414115/1‑150 (MQ=255)

                                                                                                                                      ttGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGc            >  2:1369522/1‑150 (MQ=255)

                                                                                                                                      ttGTGCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGc            >  2:49651/1‑150 (MQ=255)

                                                                                                                                          gCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATgg        <  1:1956907/150‑1 (MQ=255)

                                                                                                                                          gCCAAAGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATgg        >  1:2559592/1‑150 (MQ=255)

                                                                                                                                             aaaGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGgcg     >  2:669923/1‑150 (MQ=255)

                                                                                                                                              aaGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGgcgc    >  2:1799218/1‑150 (MQ=255)

                                                                                                                                               aGGCTAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCa   >  1:1906480/1‑150 (MQ=255)

TGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCAC  >  CP009273/4609396‑4609689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`