Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 400,594 G→T V169V (GTC→GTA)  proC ← pyrroline‑5‑carboxylate reductase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273400,5940GT99.6% 805.8 / NA 245V169V (GTC→GTA) proCpyrroline‑5‑carboxylate reductase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (136/107);  total (137/108)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

CGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGT  >  CP009273/400447‑400739
                                                                                                                                                   |                                                                                                                                                 
cGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTac                                                                                                                                                 >  2:3472190/1‑150 (MQ=255)
  tttCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTacca                                                                                                                                               >  2:2277360/1‑150 (MQ=255)
    tCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTaccacc                                                                                                                                             <  1:3119970/150‑1 (MQ=255)
     ccAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCg                                                                                                                                            >  1:2773542/1‑150 (MQ=255)
       aGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCggg                                                                                                                                          >  1:738610/1‑150 (MQ=255)
        gCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGt                                                                                                                                         <  2:3139349/150‑1 (MQ=255)
        gCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGt                                                                                                                                         >  1:325057/1‑150 (MQ=255)
          aCCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTgg                                                                                                                                       <  2:785008/150‑1 (MQ=255)
          aCCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTgg                                                                                                                                       <  2:160985/150‑1 (MQ=255)
           ccATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGa                                                                                                                                      >  1:1580202/1‑150 (MQ=255)
           ccATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGa                                                                                                                                      >  2:172653/1‑150 (MQ=255)
             aTTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGatc                                                                                                                                    <  2:1777628/150‑1 (MQ=255)
                 ttGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCg                                                                                                                                <  2:796810/150‑1 (MQ=255)
                  tGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCgg                                                                                                                               >  1:204718/1‑150 (MQ=255)
                   gCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGc                                                                                                                              >  2:2528902/1‑150 (MQ=255)
                    cGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCt                                                                                                                             >  1:1449777/1‑150 (MQ=255)
                      gAACCCATTACCGCCTGAGCGTCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAAACGCTCACACCTACCACAGGGTTGATCATCGGCTCa                                                                                                                           <  2:3713092/150‑1 (MQ=255)
                      gAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCa                                                                                                                           >  1:224790/1‑150 (MQ=255)
                      gAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCa                                                                                                                           >  1:1596052/1‑150 (MQ=255)
                       aaCCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAg                                                                                                                          >  1:1639905/1‑150 (MQ=255)
                        aCCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGc                                                                                                                         <  2:545787/150‑1 (MQ=255)
                         cccATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCa                                                                                                                        >  1:3716102/1‑150 (MQ=255)
                           cATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAAt                                                                                                                      >  1:1950607/1‑150 (MQ=255)
                           cATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAAt                                                                                                                      <  2:416627/150‑1 (MQ=255)
                            aTTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAAtt                                                                                                                     <  1:3588620/150‑1 (MQ=255)
                             ttACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTa                                                                                                                    >  1:3157898/1‑150 (MQ=255)
                             ttACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTa                                                                                                                    >  1:585908/1‑150 (MQ=255)
                              tACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTAc                                                                                                                   <  2:502509/150‑1 (MQ=255)
                                ccGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACtt                                                                                                                 <  1:2145365/150‑1 (MQ=255)
                                ccGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACtt                                                                                                                 <  2:1243139/150‑1 (MQ=255)
                                 cGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTc                                                                                                                <  2:1695946/150‑1 (MQ=255)
                                 cGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTc                                                                                                                <  2:1534444/150‑1 (MQ=255)
                                  gCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTcc                                                                                                               >  1:3109364/1‑150 (MQ=255)
                                  gCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTcc                                                                                                               <  1:301097/150‑1 (MQ=255)
                                     tgcgCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCt                                                                                                            >  1:3427388/4‑150 (MQ=255)
                                     tGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCt                                                                                                            >  1:1529961/1‑150 (MQ=255)
                                       aGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTc                                                                                                          <  1:1958513/150‑1 (MQ=255)
                                       aGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTc                                                                                                          <  2:2659233/150‑1 (MQ=255)
                                         cGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCAACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGc                                                                                                        >  1:615242/1‑150 (MQ=255)
                                          ggCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGcc                                                                                                       >  1:2304669/1‑150 (MQ=255)
                                            cAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCaa                                                                                                     >  2:3528973/1‑150 (MQ=255)
                                             aaaTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCaaa                                                                                                    >  2:2676865/1‑150 (MQ=255)
                                             aaaTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCaaa                                                                                                    >  1:247034/1‑150 (MQ=255)
                                             aaaTTTATACGCCTGGGCGCGGGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGTTTCGATAAACATAAATTCGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATAGGCTCAGCAATTACTTCCGCTTCGCCaac                                                                                                    <  1:2981889/150‑2 (MQ=255)
                                               aTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAagc                                                                                                  >  2:3214948/1‑150 (MQ=255)
                                                    taCGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCgg                                                                                             <  2:1309100/150‑1 (MQ=255)
                                                         cTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAat                                                                                        <  2:1670216/150‑1 (MQ=255)
                                                          tGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAata                                                                                       <  1:814733/150‑1 (MQ=255)
                                                           gggCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAatat                                                                                      <  1:172653/150‑1 (MQ=255)
                                                           gggCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAatat                                                                                      <  2:325055/150‑1 (MQ=255)
                                                            ggCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATAtt                                                                                     >  1:2903896/1‑150 (MQ=255)
                                                             gcgcGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTc                                                                                    <  1:475790/150‑1 (MQ=255)
                                                              cgcgTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCa                                                                                   <  2:1882309/150‑1 (MQ=255)
                                                               gcgTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAg                                                                                  <  2:1992635/150‑1 (MQ=255)
                                                                      aTCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCa                                                                           >  2:110378/1‑150 (MQ=255)
                                                                      aTCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCa                                                                           <  1:968605/150‑1 (MQ=255)
                                                                      aTCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCa                                                                           >  2:2611474/1‑150 (MQ=255)
                                                                      aTCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCa                                                                           >  2:2586526/1‑150 (MQ=255)
                                                                       tCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGACAATATTCAGCACATCAg                                                                          >  1:719861/1‑150 (MQ=255)
                                                                       tCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAg                                                                          >  1:2556986/1‑150 (MQ=255)
                                                                            cccAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTa                                                                     <  1:418463/150‑1 (MQ=255)
                                                                            cccAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTa                                                                     <  1:2324664/150‑1 (MQ=255)
                                                                             ccAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAt                                                                    >  1:3750887/1‑150 (MQ=255)
                                                                              cAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAtc                                                                   <  1:2469690/150‑1 (MQ=255)
                                                                               aGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAtct                                                                  <  1:696185/150‑1 (MQ=255)
                                                                                            cGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCa                                                     >  1:784686/1‑150 (MQ=255)
                                                                                             ggCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAg                                                    <  1:2528902/150‑1 (MQ=255)
                                                                                                cATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAgcgc                                                 >  2:2845483/1‑150 (MQ=255)
                                                                                                  tCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGt                                               >  2:226705/1‑150 (MQ=255)
                                                                                                  tCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGt                                               <  1:3518795/150‑1 (MQ=255)
                                                                                                   cGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGtt                                              <  2:1064019/150‑1 (MQ=255)
                                                                                                    gCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGttt                                             >  1:2228206/1‑150 (MQ=255)
                                                                                                      ttCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTgg                                           >  2:1338135/1‑150 (MQ=255)
                                                                                                        cGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCg                                         >  2:2600755/1‑150 (MQ=255)
                                                                                                        cGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCg                                         <  1:1377545/150‑1 (MQ=255)
                                                                                                          aTAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGtt                                       >  1:1422481/1‑150 (MQ=255)
                                                                                                          aTAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCTATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGtt                                       <  2:3477451/150‑1 (MQ=255)
                                                                                                            aaaCATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTAc                                     >  2:3068554/1‑150 (MQ=255)
                                                                                                              aCATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACgg                                   >  1:3068624/1‑150 (MQ=255)
                                                                                                                aTAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAg                                 <  2:855048/150‑1 (MQ=255)
                                                                                                                   aaTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTc                              <  2:2349412/150‑1 (MQ=255)
                                                                                                                   aaTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTc                              >  1:1793423/1‑150 (MQ=255)
                                                                                                                    aTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCa                             >  1:1044845/1‑150 (MQ=255)
                                                                                                                      aCGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATc                           <  1:860398/150‑1 (MQ=255)
                                                                                                                      aCGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATc                           <  2:1803311/150‑1 (MQ=255)
                                                                                                                       cGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATcc                          <  2:3593669/150‑1 (MQ=255)
                                                                                                                        gTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATccc                         >  1:2617040/1‑150 (MQ=255)
                                                                                                                         tAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCg                        <  2:1147902/150‑1 (MQ=255)
                                                                                                                         tAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCg                        >  2:831976/1‑150 (MQ=255)
                                                                                                                           ggCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGc                      >  2:1599576/1‑150 (MQ=255)
                                                                                                                             cTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCAt                    >  2:3683244/1‑150 (MQ=255)
                                                                                                                             cTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCAt                    >  1:1171864/1‑150 (MQ=255)
                                                                                                                                 cGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAc                <  1:3205713/150‑1 (MQ=255)
                                                                                                                                  gaagaaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAcc               >  2:1516569/1‑150 (MQ=255)
                                                                                                                                  gaagaaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAcc               <  2:1009307/150‑1 (MQ=255)
                                                                                                                                  gaagaaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAcc               >  1:1157224/1‑150 (MQ=255)
                                                                                                                                      aaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTg           >  2:2965385/1‑150 (MQ=255)
                                                                                                                                      aaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTg           >  2:585183/1‑150 (MQ=255)
                                                                                                                                          gCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCggg       <  2:1580202/150‑1 (MQ=255)
                                                                                                                                           cTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGa      >  2:3737315/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGT  >  CP009273/400447‑400739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑