Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 459,325 T→A intergenic (+83/‑68) ppiD → / → ybaV periplasmic folding chaperone, has an inactive PPIase domain/putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273459,3250TA100.0% 709.3 / NA 215intergenic (+83/‑68)ppiD/ybaVperiplasmic folding chaperone, has an inactive PPIase domain/putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (101/114);  total (101/114)

Only 100 of 215 total aligned reads displayed.

TCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAG  >  CP009273/459179‑459471
                                                                                                                                                  |                                                                                                                                                  
tctGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGcc                                                                                                                                                 <  1:2671211/150‑1 (MQ=255)
tctGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGcc                                                                                                                                                 <  1:737982/150‑1 (MQ=255)
  tgatgaGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCAt                                                                                                                                               >  1:636524/1‑150 (MQ=255)
    atgaGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTTGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCAttt                                                                                                                                             <  1:813353/150‑1 (MQ=255)
      gaGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGt                                                                                                                                           <  1:958945/150‑1 (MQ=255)
         tAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTa                                                                                                                                        <  1:3524920/150‑1 (MQ=255)
           aCCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACt                                                                                                                                      >  2:2949180/1‑150 (MQ=255)
            ccTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTg                                                                                                                                     <  2:2029310/150‑1 (MQ=255)
              tGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGtt                                                                                                                                   >  1:3739345/1‑150 (MQ=255)
              tGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGtt                                                                                                                                   >  2:1246651/1‑150 (MQ=255)
                cGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGtttt                                                                                                                                 <  1:3513979/150‑1 (MQ=255)
                cGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGtttt                                                                                                                                 >  1:978807/1‑150 (MQ=255)
                cGTAAAGAGGCGAAAATCAAAATTGGCGACGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGtttt                                                                                                                                 <  2:1146791/150‑1 (MQ=255)
                 gTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTc                                                                                                                                >  2:3740013/1‑150 (MQ=255)
                   aaaGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCAc                                                                                                                              <  2:1505118/150‑1 (MQ=255)
                   aaaGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCAc                                                                                                                              >  2:2702593/1‑150 (MQ=255)
                    aaGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACt                                                                                                                             <  2:1497119/150‑1 (MQ=255)
                    aaGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACt                                                                                                                             <  2:2217377/150‑1 (MQ=255)
                       agGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGcc                                                                                                                          <  1:2398440/150‑1 (MQ=255)
                       agGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGcc                                                                                                                          >  2:321893/1‑150 (MQ=255)
                             aaaTCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAg                                                                                                                    <  1:3580502/150‑1 (MQ=255)
                             aaaTCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTACTGAACATAGCCATATGTTTACTGTTTTCACTGCCGTTAAg                                                                                                                    >  1:126767/1‑150 (MQ=255)
                              aaTCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAgg                                                                                                                   >  1:1831936/1‑150 (MQ=255)
                                  aaaaTTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGAt                                                                                                               >  1:2780843/1‑150 (MQ=255)
                                        ggCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACt                                                                                                         <  1:1137359/150‑1 (MQ=255)
                                           gATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGtt                                                                                                      <  2:876014/150‑1 (MQ=255)
                                           gATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGtt                                                                                                      <  1:3769617/150‑1 (MQ=255)
                                           gATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGtt                                                                                                      >  2:3077070/1‑150 (MQ=255)
                                               cgcTGGAACAGCAATACTCCTGACGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGCACCGAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTaaca                                                                                                  <  1:1019974/150‑1 (MQ=255)
                                                 cTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTaacaaa                                                                                                >  2:2187760/1‑150 (MQ=255)
                                                  tGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTaacaaac                                                                                               <  1:1072519/150‑1 (MQ=255)
                                                  tGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTaacaaac                                                                                               <  1:3268217/150‑1 (MQ=255)
                                                   ggAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTaacaaaca                                                                                              <  2:3402414/150‑1 (MQ=255)
                                                    gAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAacaaacaa                                                                                             >  2:1070356/1‑150 (MQ=255)
                                                     aaCAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAg                                                                                            <  2:62731/150‑1 (MQ=255)
                                                     aaCAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAg                                                                                            <  1:2071378/150‑1 (MQ=255)
                                                       cAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGga                                                                                          <  2:2125896/150‑1 (MQ=255)
                                                          cAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGaa                                                                                       >  2:625498/1‑150 (MQ=255)
                                                            ataatCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGaaaa                                                                                     >  2:3235689/1‑150 (MQ=255)
                                                              aatCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACa                                                                                   <  2:682489/150‑1 (MQ=255)
                                                              aatCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACa                                                                                   <  2:1331342/150‑1 (MQ=255)
                                                              aatCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACa                                                                                   >  1:3796637/1‑150 (MQ=255)
                                                                  cTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTAt                                                                               <  2:102737/150‑1 (MQ=255)
                                                                   tGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTTTAACAAGAAAATGTCTCTTTCGCGGTCTTTTCAATTTCTGAACATAGCCATTTTTTTACTGTTTTCACTGCAGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATg                                                                              <  1:175578/150‑1 (MQ=255)
                                                                   tGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATg                                                                              <  1:1947133/150‑1 (MQ=255)
                                                                      aGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGaaa                                                                           <  1:2221897/150‑1 (MQ=255)
                                                                      aGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGaaa                                                                           <  1:311847/150‑1 (MQ=255)
                                                                       gccgccTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAac                                                                          <  1:1506388/150‑1 (MQ=255)
                                                                       gccgccTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAac                                                                          <  1:3415541/150‑1 (MQ=255)
                                                                        ccgccTCGCATAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAaca                                                                         <  2:2258371/150‑1 (MQ=255)
                                                                        ccgccTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAaca                                                                         >  2:2226990/1‑150 (MQ=255)
                                                                        ccgccTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAaca                                                                         >  2:3224349/1‑150 (MQ=255)
                                                                         cgccTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAacac                                                                        >  2:1627753/1‑150 (MQ=255)
                                                                           ccTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACgg                                                                      >  1:136490/1‑150 (MQ=255)
                                                                                cAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTa                                                                 <  2:3777387/150‑1 (MQ=255)
                                                                                cAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTa                                                                 <  1:2575394/150‑1 (MQ=255)
                                                                                 aaaaaaaTGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTaa                                                                <  2:636565/150‑1 (MQ=255)
                                                                                  aaaaaaTGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTaaa                                                               <  1:3254801/150‑1 (MQ=255)
                                                                                    aaaaTGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGc                                                             <  2:315235/150‑1 (MQ=255)
                                                                                      aaTGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCAc                                                           >  2:3658985/1‑150 (MQ=255)
                                                                                      aaTGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCAc                                                           >  2:3659092/1‑150 (MQ=255)
                                                                                        tGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTg                                                         <  1:1681775/150‑1 (MQ=255)
                                                                                          cGTTGCAGCTGTAACAAGAATAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACCTAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCt                                                       <  2:2377201/150‑1 (MQ=255)
                                                                                           gTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTc                                                      <  1:2935649/150‑1 (MQ=255)
                                                                                            ttGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCa                                                     >  1:2677090/1‑150 (MQ=255)
                                                                                            ttGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTCCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCa                                                     >  1:420402/1‑150 (MQ=255)
                                                                                             tGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCAt                                                    <  1:2070662/150‑1 (MQ=255)
                                                                                               cAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTa                                                  >  2:1804072/1‑150 (MQ=255)
                                                                                                  cTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTAccc                                               <  1:34321/150‑1 (MQ=255)
                                                                                                    gTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTg                                             >  2:1743988/1‑150 (MQ=255)
                                                                                                    gTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTg                                             <  1:2356633/150‑1 (MQ=255)
                                                                                                     tAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGt                                            <  1:220438/150‑1 (MQ=255)
                                                                                                       aCAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTcc                                          >  2:2248246/1‑150 (MQ=255)
                                                                                                        cAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTccc                                         <  2:2742061/150‑1 (MQ=255)
                                                                                                         aaGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCt                                        >  2:750665/1‑150 (MQ=255)
                                                                                                         aaGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCt                                        >  2:2820757/1‑150 (MQ=255)
                                                                                                         aaGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCt                                        <  1:1286463/150‑1 (MQ=255)
                                                                                                          aGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTg                                       <  2:2033418/150‑1 (MQ=255)
                                                                                                           gAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTgg                                      <  1:3172653/150‑1 (MQ=255)
                                                                                                           gAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTgg                                      >  1:1801791/1‑150 (MQ=255)
                                                                                                            aaaaGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGc                                     <  1:388429/150‑1 (MQ=255)
                                                                                                              aaGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCt                                   >  1:3621431/1‑150 (MQ=255)
                                                                                                              aaGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCt                                   <  2:2859917/150‑1 (MQ=255)
                                                                                                              aaGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAATATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCt                                   >  1:2747057/1‑150 (MQ=255)
                                                                                                                   cGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGcc                              >  2:3435315/1‑150 (MQ=255)
                                                                                                                   cGCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGcc                              <  1:1919601/150‑1 (MQ=255)
                                                                                                                    gCTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCg                             >  1:3170747/1‑150 (MQ=255)
                                                                                                                     cTTTCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCgg                            >  1:1879628/1‑150 (MQ=255)
                                                                                                                      tttCGCGGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGa                           >  1:1218649/1‑150 (MQ=255)
                                                                                                                         cgcgGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATg                        >  1:487170/1‑150 (MQ=255)
                                                                                                                          gcgGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTCTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGt                       <  2:1275126/150‑1 (MQ=255)
                                                                                                                           cgGCCTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGtc                      >  2:2404043/1‑150 (MQ=255)
                                                                                                                               cTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCAt                  <  2:3708613/150‑1 (MQ=255)
                                                                                                                               cTTTTCCATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCAt                  >  2:461898/1‑150 (MQ=255)
                                                                                                                                    ccATTTCTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAgcgc             >  2:2432954/1‑150 (MQ=255)
                                                                                                                                          cTGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTggc       <  2:3738662/150‑1 (MQ=255)
                                                                                                                                           tGAACATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTggcg      <  1:321893/150‑1 (MQ=255)
                                                                                                                                             aaCATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGAGCCGGAATGTCTCATAGCGCGCTggcggc    >  2:1982516/1‑150 (MQ=255)
                                                                                                                                              aCATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCa   <  1:1246651/150‑1 (MQ=255)
                                                                                                                                              aCATAGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCa   >  2:2954405/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAACAAGAAAAGGTCGCTTTCGCGGCCTTTTCCATTTCTGAACATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAG  >  CP009273/459179‑459471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑