BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	490,022	C→A	I54I (ATC→ATA)	recR →	gap repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	490,022	0	C	A	99.6%	915.4 / NA	274	I54I (ATC→ATA)	recR	gap repair protein
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (111/162); total (111/163)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

GCTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATCGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTTCAGG  >  CP009273/489875‑490168

 cTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGc                                                                                                                                                 <  2:260332/150‑1 (MQ=255)

   gTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCa                                                                                                                                               >  1:3524261/1‑150 (MQ=255)

     tAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACt                                                                                                                                             <  2:3413/150‑1 (MQ=255)

     tAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACt                                                                                                                                             <  2:659411/150‑1 (MQ=255)

      aaCACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTg                                                                                                                                            >  2:1665221/1‑150 (MQ=255)

          caGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGcc                                                                                                                                        <  2:3709859/150‑1 (MQ=255)

           aGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCg                                                                                                                                       <  1:2496869/150‑1 (MQ=255)

             cTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGAt                                                                                                                                     <  1:3600897/150‑1 (MQ=255)

              ttATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGAtt                                                                                                                                    <  2:179440/150‑1 (MQ=255)

              ttATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGAtt                                                                                                                                    >  1:278248/1‑150 (MQ=255)

              ttAGGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGAtt                                                                                                                                    <  1:1064165/150‑1 (MQ=255)

                 tGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGcc                                                                                                                                 <  1:2919475/150‑1 (MQ=255)

                    aaGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCa                                                                                                                              >  1:3286940/1‑150 (MQ=255)

                    aaGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCa                                                                                                                              <  2:3465804/150‑1 (MQ=255)

                      gCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACt                                                                                                                            <  1:3566191/150‑1 (MQ=255)

                      gCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACt                                                                                                                            <  1:3414354/150‑1 (MQ=255)

                        aCTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACttt                                                                                                                          <  2:3266855/150‑1 (MQ=255)

                        aCTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACttt                                                                                                                          >  1:75920/1‑150 (MQ=255)

                        aCTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATACGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACttt                                                                                                                          >  2:3227305/1‑150 (MQ=255)

                         cTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTc                                                                                                                         <  1:2082012/150‑1 (MQ=255)

                            cgcTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCAcc                                                                                                                      <  1:333490/150‑1 (MQ=255)

                            cgcTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCAcc                                                                                                                      <  1:1452205/150‑1 (MQ=255)

                             gcTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCg                                                                                                                     >  2:2283787/1‑150 (MQ=255)

                              cTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGa                                                                                                                    <  2:1634812/150‑1 (MQ=255)

                              cTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGa                                                                                                                    >  2:2675651/1‑150 (MQ=255)

                              cTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGa                                                                                                                    >  2:174891/1‑150 (MQ=255)

                              cTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGa                                                                                                                    <  2:2227405/150‑1 (MQ=255)

                               tGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGac                                                                                                                   >  2:2465165/1‑149 (MQ=255)

                               tGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGaa                                                                                                                   >  2:558891/1‑150 (MQ=255)

                               tGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGaa                                                                                                                   >  2:2926452/1‑150 (MQ=255)

                               tGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGaa                                                                                                                   >  2:1024975/1‑150 (MQ=255)

                                gTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAAc                                                                                                                  >  2:2543100/1‑150 (MQ=255)

                                gTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAAc                                                                                                                  <  2:365246/150‑1 (MQ=255)

                                gTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAAc                                                                                                                  <  2:325426/150‑1 (MQ=255)

                                 tCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACa                                                                                                                 <  2:886107/150‑1 (MQ=255)

                                  cTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAg                                                                                                                <  1:1195643/150‑1 (MQ=255)

                                   tGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAgg                                                                                                               <  2:506823/150‑1 (MQ=255)

                                   tGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAgg                                                                                                               <  2:3681785/150‑1 (MQ=255)

                                    gCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGa                                                                                                              <  1:3099911/150‑1 (MQ=255)

                                     ccGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGaa                                                                                                             <  2:2079901/150‑1 (MQ=255)

                                     ccGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGaa                                                                                                             <  1:2311512/150‑1 (MQ=255)

                                      cGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAg                                                                                                            <  1:3298461/150‑1 (MQ=255)

                                       gggCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGt                                                                                                           >  1:3608446/1‑150 (MQ=255)

                                         gCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCt                                                                                                         <  1:848410/150‑1 (MQ=255)

                                         gCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCt                                                                                                         >  1:1904487/1‑150 (MQ=255)

                                          cGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTg                                                                                                        >  2:2373450/1‑150 (MQ=255)

                                          cGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTg                                                                                                        <  2:772304/150‑1 (MQ=255)

                                           gTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGt                                                                                                       >  2:1465383/1‑150 (MQ=255)

                                           gTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGt                                                                                                       >  2:1381382/1‑150 (MQ=255)

                                              ggCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAAc                                                                                                    <  2:701720/150‑1 (MQ=255)

                                              ggCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAAc                                                                                                    >  1:243815/1‑150 (MQ=255)

                                               gCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACa                                                                                                   <  1:2574288/150‑1 (MQ=255)

                                                   gAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTg                                                                                               <  1:2265568/150‑1 (MQ=255)

                                                      gTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTc                                                                                            <  2:2724228/150‑1 (MQ=255)

                                                      gTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTc                                                                                            <  1:1302415/150‑1 (MQ=255)

                                                      gTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTc                                                                                            <  1:3201812/150‑1 (MQ=255)

                                                         ggCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGaa                                                                                         >  2:2420113/1‑150 (MQ=255)

                                                          gcgcAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAAt                                                                                        <  2:3325409/150‑1 (MQ=255)

                                                                      gCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAg                                                                            <  1:1762716/150‑1 (MQ=255)

                                                                         ttCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGaa                                                                         <  2:249079/150‑1 (MQ=255)

                                                                            aCGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAAc                                                                      <  1:1676877/150‑1 (MQ=255)

                                                                              gctgctTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACgg                                                                    <  1:2666315/150‑1 (MQ=255)

                                                                                 gctTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCa                                                                 <  1:2862480/150‑1 (MQ=255)

                                                                                 gctTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCa                                                                 <  1:959863/150‑1 (MQ=255)

                                                                                      aGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCt                                                            <  1:1665221/150‑1 (MQ=255)

                                                                                      aGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCt                                                            <  2:2963468/150‑1 (MQ=255)

                                                                                        cgcgATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGc                                                          <  2:2271744/150‑1 (MQ=255)

                                                                                         gcgATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCg                                                         <  2:2398983/150‑1 (MQ=255)

                                                                                           gATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCgtg                                                       >  2:638516/1‑150 (MQ=255)

                                                                                               gTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGtggtgg                                                   >  2:95901/1‑150 (MQ=255)

                                                                                               gTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGtggtgg                                                   <  2:1227664/150‑1 (MQ=255)

                                                                                                tAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGga                                                  >  2:1074143/1‑150 (MQ=255)

                                                                                                 aGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGgag                                                 <  1:1622100/150‑1 (MQ=255)

                                                                                                   cggcggGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGagag                                               >  2:3003277/1‑150 (MQ=255)

                                                                                                    ggcggGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGt                                              >  1:2116119/1‑150 (MQ=255)

                                                                                                     gcggGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTc                                             >  2:1249043/1‑150 (MQ=255)

                                                                                                       gggATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCcg                                           <  1:1935949/150‑1 (MQ=255)

                                                                                                       gggATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCcg                                           >  2:588993/1‑150 (MQ=255)

                                                                                                           tGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCcggcgg                                       <  1:2614689/150‑1 (MQ=255)

                                                                                                               tCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACAt                                   >  1:2221778/1‑150 (MQ=255)

                                                                                                                cTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATc                                  <  1:1294859/150‑1 (MQ=255)

                                                                                                                            cTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAg                      <  2:3294437/150‑1 (MQ=255)

                                                                                                                            cTCACCCGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAg                      <  2:3548522/150‑1 (MQ=255)

                                                                                                                                cccGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGa                  <  2:1967152/150‑1 (MQ=255)

                                                                                                                                 ccGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGAc                 >  1:3229274/1‑150 (MQ=255)

                                                                                                                                 ccGGGCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGAc                 <  1:103452/150‑1 (MQ=255)

                                                                                                                                    ggCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACggg              <  2:279754/150‑1 (MQ=255)

                                                                                                                                     gCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACgggg             <  2:240327/150‑1 (MQ=255)

                                                                                                                                     gCGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACgggg             <  1:2285518/150‑1 (MQ=255)

                                                                                                                                      cGATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGc            <  1:19889/150‑1 (MQ=255)

                                                                                                                                       gATGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCa           >  1:477007/1‑150 (MQ=255)

                                                                                                                                        aTGTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGTGGCAg          >  1:1361672/1‑150 (MQ=255)

                                                                                                                                          gTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGtt        <  2:1648949/150‑1 (MQ=255)

                                                                                                                                          gTCGGAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGtt        <  1:1820554/150‑1 (MQ=255)

                                                                                                                                             ggAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGGGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTTc     >  2:1306312/1‑150 (MQ=255)

                                                                                                                                             ggAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTTc     >  1:2525611/1‑150 (MQ=255)

                                                                                                                                             ggAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTTc     >  1:618279/1‑150 (MQ=255)

                                                                                                                                             ggAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTTc     <  2:2656546/150‑1 (MQ=255)

                                                                                                                                              gAAATAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTTCa    <  2:2292696/150‑1 (MQ=255)

                                                                                                                                                aaTAGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTAGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTTCAgg  >  2:1711708/1‑150 (MQ=255)

GCTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATCGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTTCAGG  >  CP009273/489875‑490168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`