Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 503,944 G→T E91* (GAA→TAA)  ybaQ → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273503,9440GT95.4% 622.3 / ‑2.6 216E91* (GAA→TAA) ybaQputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (107/99);  minor base A (10/0);  total (117/99)
Fisher's exact test for biased strand distribution p-value = 2.12e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

GTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTG  >  CP009273/503798‑504090
                                                                                                                                                  |                                                                                                                                                  
 tGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAAtt                                                                                                                                                <  2:72392/150‑1 (MQ=255)
 tGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAAtt                                                                                                                                                <  2:2786093/150‑1 (MQ=255)
  gCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTg                                                                                                                                               >  2:2065436/1‑150 (MQ=255)
  gCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTGTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACACCATGCGCACCCAGGGATAATTg                                                                                                                                               >  2:3550567/1‑150 (MQ=255)
   cACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTgg                                                                                                                                              >  2:1957870/1‑150 (MQ=255)
   cACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTgg                                                                                                                                              >  1:2508328/1‑150 (MQ=255)
   cACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTgg                                                                                                                                              <  2:2192083/150‑1 (MQ=255)
      tGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCACCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGAc                                                                                                                                           >  1:1511577/1‑150 (MQ=255)
        aTCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACgg                                                                                                                                         <  2:2723919/150‑1 (MQ=255)
           aaTAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAAGCGGCGGTTGATCTTTGGGAAATTGAAAACAACAAGCGCACCCAGGAATAATTGGGACGGAtt                                                                                                                                      >  1:1133349/1‑150 (MQ=255)
            aTAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTg                                                                                                                                     >  2:3241425/1‑150 (MQ=255)
            aTAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTg                                                                                                                                     <  1:3457908/150‑1 (MQ=255)
            aTAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTg                                                                                                                                     >  1:594953/1‑150 (MQ=255)
            aTAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCACCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACAAGCGCACCCAGGAATAATTGGGACGGATTg                                                                                                                                     >  1:3045208/1‑150 (MQ=255)
                cAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTACAAGCGGCGGTTGATAATTGGGAAATTGAAAAAAACATGCGCACCCAGGAATAATTGGGAAGGATTTAAAc                                                                                                                                 >  1:1020660/1‑150 (MQ=255)
                 aaTCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACa                                                                                                                                <  2:3209685/150‑1 (MQ=255)
                   tcttAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGt                                                                                                                              <  2:2014861/147‑1 (MQ=255)
                      tAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGc                                                                                                                           >  2:1722476/1‑150 (MQ=255)
                      tAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATAATTTGCTAAAACACCAAGAAGCAGTTAATCATTATGAAATTTAAAACAACAAGCGCAACCCAGAATAATTTGTACGAAATTAAACAGTGGc                                                                                                                           >  1:3587644/1‑150 (MQ=255)
                       aaaCTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACAACCAAGCGGCGGTTGATATTTTGGAAATTTAAAACAACAAGCGCACACAGGAATAATTTGGACGGATTGAAACAATAGCt                                                                                                                          >  1:2632323/1‑150 (MQ=255)
                       aaaCTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCt                                                                                                                          <  1:3191035/150‑1 (MQ=255)
                           tCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAat                                                                                                                      >  1:1204747/1‑150 (MQ=255)
                                actGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCACCAGGCGGCGGTTGATATTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAATTGCTGAATATTTg                                                                                                                 >  1:3635899/1‑150 (MQ=255)
                                  tGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGc                                                                                                               <  1:1610813/150‑1 (MQ=255)
                                  tGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGc                                                                                                               <  2:1897143/150‑1 (MQ=255)
                                     gaTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAAACAACATGAGGCGGTTGTTCTTTTGGAAGTTGAAAACAAAATGCGCACCCAGGAAAAAATGGGAAGGATTGAAACCGTGGCTGAATATTTGGCACg                                                                                                            >  1:866362/1‑150 (MQ=255)
                                          cATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTg                                                                                                       <  2:282463/150‑1 (MQ=255)
                                          cATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAAACTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTg                                                                                                       >  2:3346114/1‑150 (MQ=255)
                                           aTTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCATTTGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTTGCACGCCGTGa                                                                                                      >  1:3105882/1‑150 (MQ=255)
                                             ttCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAag                                                                                                    >  2:3616442/1‑150 (MQ=255)
                                                gTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCACCAGGCGGCGGTTGTTCTTTTTGAATTTGAAAAAAACCAGCGCACCCAAGAAAAATTGGGACGGATTGAAACAATTGCTGAATATTTTGCACGCCCTGAagata                                                                                                 >  1:2614488/1‑148 (MQ=255)
                                                  cTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTTATCTTTGGGAAATTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGt                                                                                               >  1:1702988/1‑150 (MQ=255)
                                                  cTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGt                                                                                               <  2:1377743/150‑1 (MQ=255)
                                                       gAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCaaa                                                                                          >  2:1813904/1‑150 (MQ=255)
                                                         aaGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCaaaaa                                                                                        <  2:2911992/150‑1 (MQ=255)
                                                          aGTTTTTGATACCACAGTCGATTTTTGGGTAAACCTCCAGGCGGCGGTTGATATTTGGGAAGTTGAAAACAACATGCGCACCCACGAATAATTGGGACGGCTTGAAACAGAGGCGGAATATTTGGCACGCCGTGAAGAGCGTGCaaaaaa                                                                                       >  2:983100/1‑150 (MQ=255)
                                                            tttttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAgg                                                                                     <  1:2951743/150‑1 (MQ=255)
                                                             ttttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGt                                                                                    <  2:800849/150‑1 (MQ=255)
                                                              tttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTc                                                                                   <  1:2533340/150‑1 (MQ=255)
                                                                tGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTTAAAACAACATGCGCACCCAGGAAAAATTGGGACGGATTGAAACAGTGGCTGAATATTTTGCACACCATGAAGAGTGTGCAAAAAATGTcgc                                                                                 >  1:2793092/1‑150 (MQ=255)
                                                                 gATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGATcgcg                                                                                <  2:952818/150‑1 (MQ=255)
                                                                 gATACCACAGTCGATTTTTGGCTAAACCACCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTcgcg                                                                                >  2:671994/1‑150 (MQ=255)
                                                                   tACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTa                                                                              >  2:1562529/1‑150 (MQ=255)
                                                                   tACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTa                                                                              <  2:2840651/150‑1 (MQ=255)
                                                                    aCCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGGaa                                                                             >  2:3661110/1‑150 (MQ=255)
                                                                         aGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCa                                                                        >  1:3160584/1‑150 (MQ=255)
                                                                          gTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaa                                                                       <  1:3579709/150‑1 (MQ=255)
                                                                          gTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaa                                                                       >  1:184768/1‑150 (MQ=255)
                                                                           tCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaaa                                                                      <  1:2755229/150‑1 (MQ=255)
                                                                            cGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaaaa                                                                     >  2:3216505/1‑150 (MQ=255)
                                                                             gATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAAc                                                                    >  1:2215376/1‑150 (MQ=255)
                                                                             gATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAAc                                                                    <  1:126525/150‑1 (MQ=255)
                                                                                  ttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGc                                                               >  2:2425821/1‑150 (MQ=255)
                                                                                   tGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCTTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCt                                                              >  1:2706661/1‑150 (MQ=255)
                                                                                   tGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCt                                                              >  1:2270138/1‑150 (MQ=255)
                                                                                     gCTAAACCTCCAGGCGGCTGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTc                                                            <  2:2067672/150‑1 (MQ=255)
                                                                                     gCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTc                                                            <  2:1133349/150‑1 (MQ=255)
                                                                                          aCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCAtctc                                                       <  2:2639972/150‑1 (MQ=255)
                                                                                          aCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCAtctc                                                       <  2:330392/150‑1 (MQ=255)
                                                                                            cTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGt                                                     >  2:1031555/1‑150 (MQ=255)
                                                                                             tCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAATCAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTa                                                    >  2:1023641/1‑150 (MQ=255)
                                                                                                aGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGc                                                 >  2:2569452/1‑150 (MQ=255)
                                                                                                aGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGc                                                 >  1:3098190/1‑150 (MQ=255)
                                                                                                aGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGc                                                 >  2:1344610/1‑150 (MQ=255)
                                                                                                  gcggcgGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCg                                               <  1:2082513/150‑1 (MQ=255)
                                                                                                   cggcggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCgg                                              >  1:465418/1‑150 (MQ=255)
                                                                                                    ggcggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGa                                             >  2:529671/1‑150 (MQ=255)
                                                                                                    ggcggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGa                                             >  2:1193910/1‑150 (MQ=255)
                                                                                                       ggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATaa                                          >  1:2103500/1‑150 (MQ=255)
                                                                                                       ggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATaa                                          <  2:2603943/150‑1 (MQ=255)
                                                                                                          tGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGc                                       >  1:1752851/1‑150 (MQ=255)
                                                                                                                ttGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAc                                 <  1:2006060/150‑1 (MQ=255)
                                                                                                                ttGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAc                                 <  2:2618529/150‑1 (MQ=255)
                                                                                                                ttGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAc                                 <  1:2796328/150‑1 (MQ=255)
                                                                                                                ttGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAc                                 >  2:86763/1‑150 (MQ=255)
                                                                                                                 tGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcg                                >  1:1434432/1‑150 (MQ=255)
                                                                                                                 tGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcg                                <  1:3744346/150‑1 (MQ=255)
                                                                                                                   ggAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGTAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcgcc                              <  2:3045208/150‑1 (MQ=255)
                                                                                                                         tGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatcc                        <  1:427159/150‑1 (MQ=255)
                                                                                                                         tGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatcc                        >  1:2345772/1‑150 (MQ=255)
                                                                                                                          gAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccg                       <  2:3587582/150‑1 (MQ=255)
                                                                                                                             aacaacCTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggca                    <  2:3635899/150‑1 (MQ=255)
                                                                                                                              acaacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcat                   >  1:390967/1‑150 (MQ=255)
                                                                                                                              acaacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcat                   >  1:391115/1‑150 (MQ=255)
                                                                                                                               caacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatc                  >  2:1239707/1‑150 (MQ=255)
                                                                                                                               caacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatc                  >  2:1878214/1‑150 (MQ=255)
                                                                                                                                aacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatcc                 <  1:3303965/150‑1 (MQ=255)
                                                                                                                                aacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatcc                 <  2:1749174/150‑1 (MQ=255)
                                                                                                                                aacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatcc                 <  2:208403/150‑1 (MQ=255)
                                                                                                                                aacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatcc                 <  2:547397/150‑1 (MQ=255)
                                                                                                                                    tGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGc             >  1:903380/1‑150 (MQ=255)
                                                                                                                                      cgcACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCAc           >  1:1320118/1‑150 (MQ=255)
                                                                                                                                      cgcACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCAc           >  1:1318815/1‑150 (MQ=255)
                                                                                                                                        cACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCa         >  2:3253967/1‑150 (MQ=255)
                                                                                                                                          cccAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCAtt       <  2:3731010/150‑1 (MQ=255)
                                                                                                                                          cccAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCAtt       >  1:2933792/1‑150 (MQ=255)
                                                                                                                                              ggAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCt   >  1:799049/1‑150 (MQ=255)
                                                                                                                                              ggAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCt   >  1:2323362/1‑150 (MQ=255)
                                                                                                                                              ggAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCt   >  2:3011598/1‑150 (MQ=255)
                                                                                                                                               gaataaTTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTg  >  2:433713/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTG  >  CP009273/503798‑504090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑