Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 524,496 T→G pseudogene (401/491 nt) ybbD → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273524,4960TG99.5% 806.2 / NA 206pseudogene (401/491 nt)ybbDpseudogene
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (103/102);  total (103/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

TTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAA  >  CP009273/524349‑524641
                                                                                                                                                   |                                                                                                                                                 
  cccTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTaa                                                                                                                                               >  1:564410/1‑150 (MQ=255)
   ccTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTaaa                                                                                                                                              <  1:2093582/150‑1 (MQ=255)
    cTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAAc                                                                                                                                             >  1:3599303/1‑150 (MQ=255)
    cTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAAc                                                                                                                                             <  1:2543375/150‑1 (MQ=255)
    cTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAAc                                                                                                                                             <  2:3570588/150‑1 (MQ=255)
       aCAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCtt                                                                                                                                          <  2:670874/150‑1 (MQ=255)
       aCAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCtt                                                                                                                                          <  1:396899/150‑1 (MQ=255)
             gatgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCtttttttt                                                                                                                                    <  1:2836474/150‑1 (MQ=255)
              atgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTttg                                                                                                                                   <  2:2646889/150‑1 (MQ=255)
              atgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTttg                                                                                                                                   >  1:2221715/1‑150 (MQ=255)
                gatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTttgtt                                                                                                                                 <  2:3778479/150‑1 (MQ=255)
                  tgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATACAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTgg                                                                                                                               <  1:2528654/150‑1 (MQ=255)
                    ggggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAATTGTGGTGTAAATATGGTAATATACCAGTGCAAGGGGATGGGGTAAACCTTTTTTTTTttgttg                                                                                                                             >  1:456377/1‑150 (MQ=255)
                      ggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGaa                                                                                                                           <  2:2919082/150‑1 (MQ=255)
                      ggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGaa                                                                                                                           <  1:1848302/150‑1 (MQ=255)
                         aGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTACGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAAtt                                                                                                                        >  1:3704172/1‑150 (MQ=255)
                         aGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAAtt                                                                                                                        >  1:1983007/1‑150 (MQ=255)
                           gTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTaa                                                                                                                      >  2:1921872/1‑150 (MQ=255)
                             ttAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATg                                                                                                                    <  2:914343/150‑1 (MQ=255)
                               aaGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGtt                                                                                                                  >  2:1261732/1‑150 (MQ=255)
                                 gAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTAc                                                                                                                >  1:2780725/1‑150 (MQ=255)
                                   aaaCATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGc                                                                                                              >  2:335599/1‑150 (MQ=255)
                                   aaaCATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGc                                                                                                              >  2:1469553/1‑150 (MQ=255)
                                     aCATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCat                                                                                                            >  1:2697840/1‑150 (MQ=255)
                                       aTTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCatta                                                                                                          <  1:106210/150‑1 (MQ=255)
                                       aTTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCatta                                                                                                          >  1:1432168/1‑150 (MQ=255)
                                       aTTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCatta                                                                                                          >  1:3095928/1‑150 (MQ=255)
                                       aTTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGAGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCatta                                                                                                          >  1:1194155/1‑150 (MQ=255)
                                         tGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCattatt                                                                                                        >  1:1761202/1‑150 (MQ=255)
                                              gTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTtata                                                                                                   >  1:409588/1‑150 (MQ=255)
                                              gTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTtata                                                                                                   <  2:2466226/150‑1 (MQ=255)
                                               tGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATaa                                                                                                  <  2:1535327/150‑1 (MQ=255)
                                                  gggCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACaa                                                                                               >  2:457997/1‑150 (MQ=255)
                                                      cAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAatat                                                                                           >  2:3316851/1‑150 (MQ=255)
                                                      cAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAatat                                                                                           <  2:1555549/150‑1 (MQ=255)
                                                         ggAACAAGTGGCGAAAATCGTATCAAAGAATGATACAGATACAAAAAAAGTGTTGTGGAAATATAATAAGATACCAGAGCAAGTGGATGGGGTAAAACATTTTTAAGATGGTGAAATTAATTATACTCATAATTTTATAACAAATATTga                                                                                        >  1:2289744/1‑149 (MQ=255)
                                                          gAACATGTGGCGAAAATCGTATCAAAGAATGAACCAGATAAAAAAAAAGTGTGTTGTAAATATGGAAATATACCAGGGCACGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGa                                                                                       <  2:3105707/150‑1 (MQ=255)
                                                          gAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGa                                                                                       <  2:202279/150‑1 (MQ=255)
                                                             cAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCt                                                                                    >  1:2939957/1‑150 (MQ=255)
                                                               aGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTgg                                                                                  <  2:86238/150‑1 (MQ=255)
                                                               aGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTgg                                                                                  <  2:1389080/150‑1 (MQ=255)
                                                               aGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTgg                                                                                  >  2:1131932/1‑150 (MQ=255)
                                                                gTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGa                                                                                 <  2:2831845/150‑1 (MQ=255)
                                                                      aaaaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCt                                                                           <  2:2221148/150‑1 (MQ=255)
                                                                       aaaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTg                                                                          <  2:3628780/150‑1 (MQ=255)
                                                                        aaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGa                                                                         >  1:3358305/1‑150 (MQ=255)
                                                                         aTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGAt                                                                        <  1:1461655/150‑1 (MQ=255)
                                                                          tCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATg                                                                       <  2:378816/150‑1 (MQ=255)
                                                                            gTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATATTATAACAAATATTGGAGCTGGATTGCCTGATGCt                                                                     >  1:1269923/1‑150 (MQ=255)
                                                                               tCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTtgt                                                                  <  1:2878668/150‑1 (MQ=255)
                                                                                cAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTtgtg                                                                 <  2:83327/150‑1 (MQ=255)
                                                                                  aaGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCa                                                               >  2:1711417/1‑150 (MQ=255)
                                                                                  aaGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCa                                                               >  2:3489578/1‑150 (MQ=255)
                                                                                      aTGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGt                                                           <  2:1332889/150‑1 (MQ=255)
                                                                                           ccAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTg                                                      <  1:215239/150‑1 (MQ=255)
                                                                                             aGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCt                                                    <  2:101218/150‑1 (MQ=255)
                                                                                                tACAAATAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGa                                                 <  2:1775291/150‑1 (MQ=255)
                                                                                                  cAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAAt                                               >  1:2467537/1‑150 (MQ=255)
                                                                                                   aaaaaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAAtt                                              >  1:318739/1‑150 (MQ=255)
                                                                                                    aaaaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTa                                             <  2:1126897/150‑1 (MQ=255)
                                                                                                     aaaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTaa                                            >  2:3275735/1‑150 (MQ=255)
                                                                                                      aaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAag                                           <  2:2183288/150‑1 (MQ=255)
                                                                                                       aaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAaga                                          >  2:1808071/1‑150 (MQ=255)
                                                                                                       aaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAaga                                          >  1:2976999/1‑150 (MQ=255)
                                                                                                        aaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAagag                                         >  2:3417235/1‑150 (MQ=255)
                                                                                                         attgtgGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGt                                        >  1:1156278/3‑150 (MQ=255)
                                                                                                         aGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGt                                        <  1:109315/150‑1 (MQ=255)
                                                                                                         aGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGt                                        >  2:75883/1‑150 (MQ=255)
                                                                                                           tgtgGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCt                                      >  2:3666155/1‑150 (MQ=255)
                                                                                                            gtggtgTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTa                                     <  1:919842/150‑1 (MQ=255)
                                                                                                               gtgtAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATcc                                  <  2:2723712/150‑1 (MQ=255)
                                                                                                               gtgtAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATcc                                  <  2:429984/150‑1 (MQ=255)
                                                                                                               gtgtAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATcc                                  >  1:1713803/1‑150 (MQ=255)
                                                                                                                  tAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCat                               <  1:659576/150‑1 (MQ=255)
                                                                                                                   aaaTATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCata                              >  2:2513481/1‑150 (MQ=255)
                                                                                                                    aaTATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCatat                             <  2:398694/150‑1 (MQ=255)
                                                                                                                     atatGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATc                            <  2:3599303/150‑1 (MQ=255)
                                                                                                                        tGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGaa                         <  1:1881565/150‑1 (MQ=255)
                                                                                                                          gTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgt                       >  1:3628669/1‑150 (MQ=255)
                                                                                                                          gTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgt                       <  1:3241534/150‑1 (MQ=255)
                                                                                                                           tAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgtc                      >  1:1840189/1‑150 (MQ=255)
                                                                                                                            aaGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgtcg                     >  1:3626942/1‑150 (MQ=255)
                                                                                                                            aaGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgtcg                     <  2:1811310/150‑1 (MQ=255)
                                                                                                                             ataTACCAGGGCATTGTTATGGTTTAAACCTTTTTTTTGTTGGTTAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgtcgt                    <  2:3698961/148‑1 (MQ=255)
                                                                                                                              gATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgtcgtc                   <  1:1188842/150‑1 (MQ=255)
                                                                                                                              gATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgtcgtc                   >  2:1508827/1‑150 (MQ=255)
                                                                                                                               aTACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCa                  >  2:746080/1‑150 (MQ=255)
                                                                                                                                tACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCaa                 <  2:59371/150‑1 (MQ=255)
                                                                                                                                   cAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACtt              <  1:457997/150‑1 (MQ=255)
                                                                                                                                    aGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTc             >  2:2005796/1‑150 (MQ=255)
                                                                                                                                    aGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTc             <  1:3077129/150‑1 (MQ=255)
                                                                                                                                     gggCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCg            <  2:175790/150‑1 (MQ=255)
                                                                                                                                     gggCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCg            <  1:1545732/150‑1 (MQ=255)
                                                                                                                                      ggCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGt           <  1:1131932/150‑1 (MQ=255)
                                                                                                                                        cAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAg         >  2:2047785/1‑150 (MQ=255)
                                                                                                                                         aaGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGt        <  2:2587945/150‑1 (MQ=255)
                                                                                                                                           ggggATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGa      >  2:3158744/1‑150 (MQ=255)
                                                                                                                                           ggggATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGa      <  1:3673251/150‑1 (MQ=255)
                                                                                                                                            gggATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCCTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAg     >  2:2938920/1‑150 (MQ=255)
                                                                                                                                              gATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGa   <  2:456377/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAA  >  CP009273/524349‑524641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑